We investigated the association of LTR element in transcript variant of MOBP gene. LTR33 belonging to the ERV1 family contributes to the change of coding region of MOBP gene by te production of splicing variant. Real-time RT-PCR analysis using different human tissues shows the dominant expression patterns in AD brain, adult whole brain, spinal cord, and testis tissues. Interestingly, comparative analysis of expression pattern using corresponding tissues between human and rhesus monkey shows the different expression patterns. To investigate the evolutionary conservation of LTR33 element of the MOBP gene, various primate DNA samples were used for the PCR amplification and sequencing. Their splicing donor site (GT) are completely conserved and the number of tandem repeated element which could be translated are increased during the evolution of hominoid primates. Taken together, integration event of LTR33 element could affect the coding capacity and expression in different tissues by changing the coding sequences during the primateevolution.
Real-time RT-PCR
PCR
INTRODUCTION
0
0.002
0.004
0.006
0.008
0.01
0.012
0.014
0.016
0.018
alzheimer adultbrain
fetal brain heart lung spinalcord
testis thyroid trachea
MO
BP_L
TR
33
/GA
PD
H
0
0.005
0.01
0.015
0.02
0.025
0.03
parietal lobe medullaoblongata
pons occipital lobe cereberalcortex
corpuscallosum
olfactory thalamus frontal lobe temporal lobe hippocampus
RESULTS & DISCUSSION
MERERIALS & METHODS
REFERENCES
www.primate.or.kr
Dual coding capacity of MOBP gene derived by the Dual coding capacity of MOBP gene derived by the insertion of retroviral LTR33 elementinsertion of retroviral LTR33 element Yu-Na NohYu-Na Noh11, Jae-Won Huh, Jae-Won Huh11, Dae-Soo Kim, Dae-Soo Kim22, Kung Ahn, Kung Ahn11, and Heui-Soo Kim, and Heui-Soo Kim1,21,2
1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 1.Division of Biological Sciences, College of Natural Sciences, Pusan National University 2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University2.PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University
ABSTRACT
[Structure of Retrovirus]
[Retrovirus life cycle]
8%
[Genome component parts][HERV Structure]
*HERV: Human Endogenous Retrovirus
Gene Cloning
BioInformatics
Primate DNA MOBP Gene
Human
Gorilla
Orangutan
Gibbon
Old World monkeys
New World monkeys
Prosimians
Million years
70 60 50 40 30 20 10
LTR33
[Figure 5. Real-time RT-PCR analysis of MOBP genetranscript]
[Figure 6.Integration lineage of LTR33 element during thePrimate evolution.]
AmplifiedPlasmid DNA
1.Yoichi Yamamoto et al. 1994,Cloning and Expression of Myelin-associated Oligodendrocytic Basic Protein, The Journalof Biological Chemistry,Vol.269:31725-317302. HOLZ A. and SCHWAB M. E.,1997,Developmental Expression of the myelin gene MOBP in the rat nervous system, Journal ofNeurocytology,26:467-477
[Figure 4.Comparative analysis of MOBP gene transcripts between Human and RhesusMonkey]
[Figure 1. Four different alternative transcripts of MOBP gene]
[Figure 3. Expression analysis of alternative transcripts in various human tissues including different braintissues.]
[Figure 2. Multiple alignment of MOBPgene.]
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