Q UANTIFYING METABOLIC FLUXES IN CANCER AND STEM CELLS An
integrative approach monitoring metabolic reprogramming Christin
Zasada Kempa Lab - Integrative Proteomics & Metabolomics
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Genome-regulatory code 2 Genomics three billion DNA base pairs
20000 - 25000 protein-coding genes Transcriptomics 2 % of the
genome Proteomics ~ 5 000 Metabolomics
Non-stationary metabolic flux analysis 5 Model parameter 168
labeling data point 5 measured rates 14 measured pool sizes in
cooperation with Katharina Nh and Sebastian Niedenfhr
Forschungszentrum Jlich GmBH Niedenfhr S, Nh K, Wiechert W Current
Opinion in Biotechnology 2015, 34:8290 Flux information InputOutput
Pathways coverage Glycolysis TCA-Cycle Pentose-Phosphate Pathway
Glutaminolysis Amino acid synthesis Biosynthesis Time courses for:
Glucose Glutamine metabolic steady & isotopic
non-stationary
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6 GC-MS fragment mapping to molecule level
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Monitoring metabolic reprogramming - experimental setup 7 13
C-Glucose 13 C-Glutamine 13 C-Pyruvate Proteomics hESC-DFhESC
MDA-MB231 DFiPSC HFF1 NFH2 BJ1 in cooperation with R. Bucowiecki
and A. Prigione (MDC, Berlin, Germany)
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Re-arrangement of carbon fate 8
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Routing of 13 C-Pyruvic acid 9 LFQ Int
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Summary 10
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Acknowledgments 11 Alessandro Prigione Raul Bukowiecki MDC
Berlin-Buch, Germany Katharina Nh Sebastian Niedenfhr
Forschungszentrum Jlich Germany thank you for your attention