1
PROBIOTICS IN HEALTH & DISEASE
www.triphasepharma.com
An ISO9001:2008 Certified Company
• What are Probiotics?
• Potential Mechanisms of Action
• Therapeutic Targets of Probiotics
• Strain Specificity
• Quality Control
Private & Confidential 2
Private & Confidential 3
PROBIOTICS
“Live microorganisms which when administered in adequate amounts confer a health benefit on the host”
Probiotic microorganisms can be found in both supplement form and as components of foods and beverages.
Private & Confidential4
TYPES OF PROBIOTIC
• Lactobacillus - Most available are species are,
- L.acidophilus - L.casei - L.reuterii - L.rhamnosus - L.bulgaricus - L.plantarum Bifidobacteria - Most available are species are, - B.infantis - B.longum - B.bifidum - B.lactis• Saccharomyces boulardii – Widely studied probiotic yeast• Saccharomyces cerevisiae- Most used probiotic strain for varied
applications
5
MECHANISM OF ACTION
6
THERAPEUTIC TARGETS OF PROBIOTICS
Gut Function
Acute Diarrhea AAD, Travelers diarrhea C. difficile Inf Lactose IndigestionIBS symptoms Gut pain sensationInflammatory bowel
Oral CareDental caries
ColdsRespiratory Infections
Skin Microbiology, Inflammation
Metabolic SyndromeObesity, Diabetes
AllergyAtopic dermatitisAsthma
Vaginal Infection
S. boulardii Is Effective in Patients with Recurrent C. difficile Disease
CDD=Clostridium difficile-associated diseaseMcFarland L, et al. JAMA. 1994;271:1913-1918.
Initial CDD
CD
D re
curr
ence
, %
S. boulardii(n=31)
Placebo(n=33)
P=NS
Recurrent CDD
Control(n=34)
P=0.04
Patients received standard antibiotics (vancomycin or metronidazole) and S. boulardii 1 g/day or placebo for 4 weeks.
CD
D re
curr
ence
, % S. boulardii
(n=26)
Mixtures included: Lactinex = L. acidophilus and L. bulgaricus; Lactobacillus acidophilus and Bifidobacterium lactis; Lactobacillus acidophilus and Bifidobacterium infantis.
McFarland, Am J Gastroenterol 2006; 101(4): 812-822.
PROBIOTICS FOR PREVENTION OF ANTIBIOTIC ASSOCIATED DIARRHEA
Probiotics are live active cultures
More is not better
Dose (1 billion vs 10 billion vs 450 billion)Number of strains
The effect of probiotics is genus specific
MISCONCEPTIONS ABOUT PROBIOTICS
DOSAGE & STRAINS OF PROBIOTICS
10
• The dose of probiotics is usually expressed as the number of colony forming units (CFUs)
• The required dose of probiotics may vary greatly for different strains and the specific health effect under investigation
• Probiotic effects should be considered dose-specific
• Dose listed on the label must be based on studies that show a health effect in humans
• Different strains of the same species can be different
• Clinical support to substantiate claims must be for each probiotic strain
PROBIOTICS -QUALITY CONTROL
11
• Source (Animal vs Human; Normal vs Diseased)
• Safety (in at risk populations)
• Characterization (strain purity)
• Viability (Cfu Delivered)
• Dose (Dose-response studies)
• Combinations/cocktails (Different effects of different Bacterial,yeast
strains)
AWARENESS OF PROBIOTICS
PROBIOTICS IN THE CURRENT MARKET PLACE
13
ADVANTAGES OF YEAST PROBIOTIC COMPARE TO BACTERIAL PROBIOTICS
14
Probiotic Characteristics Yeast
ProbioticBacterial Probiotic
Unique Advantage
Viable at a lower pH to survive gastric digestion
Yes Limited
Resistance to Antibiotics Yes No
Reaches colon in an active state Yes Limited
SafetyDoes not lead to permanent intestinal colonization
Yes Yes
Appropriate for ages 2 months and up Yes Limited
Toxin neutralization
Neutralization of Enterotoxin Yes No
Temperature stability
Required Refrigeration No Yes
www.triphasepharma.com
Thank You.
Top Related