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SHORT- STAY PROGRAM
Laboratory Of Analytical Environmental Chemistry
Faculty of Mathematics and Natural Sciences
Padang-Indonesia
Determination Cation and Anion of BiomaterialDetermination Cation and Anion of Biomaterial
(Straw of Rice)(Straw of Rice)
Using Ion Exchange ChromatographyUsing Ion Exchange Chromatography
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Waste
water
MSL
method
Introduction of My Research
The Multi-Soil-Layering (MSL) system was
composed of soil mixture layers and high
Water permeable layers which are arranged in
brick pattern
It will ca!se a "ariety of the
disease # S$in diseasesCancer %ilariasisDiare etc
If not processedIf not processedbefore usedbefore used
So needSo need
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Introduction of My Research
eolite! high "#" $ absorption of %&'
Soil! filter absorption habitat of organisms
Metal iron! fixation denitrification (reducer)*iomaterial ! denitrification (& donor)
"harcoal! absorption of organic component
habitat of organisms
Material is contained itMaterial is contained it
MSLMSL
T++LT++L
• Determination Cation and Anion ofDetermination Cation and Anion of
BiomaterialBiomaterial
(Straw of Rice) Using Ion Exchange(Straw of Rice) Using Ion Exchange
ChromatographyChromatography
&L"&L"
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Experimental
Apparatus The pump,injector, column capillery fused silica capillary
!"#$mm I% x !"&' mm (%)and the colomn len*ths were +! cm ,and detector"
Rea*ent and material
Stoc solution of-a$S(#,-aI(#,-a.r(#,-a-($,-a-(#,-aI,-aS/- Anions) and0i/l,-a/l,-1&/l,2/0,M*/l$,/a/l$ cation ) ware prepared in +!mM
/oulomn preparation
& 3used silica capillary and paced TS2*el I/4Anion45W60 and I/4
/ation4Sw
Sample
Straw of rice
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Experimental7 Sample preparation for biomaterials
.iomaterials ' *)
3iltrat
' Soa$ed with A!a regia *Cl#*+,-. -#/0(12ml3 45 ho!rs)
' %iltered with filter paper (46m3 d./1 cm)' %iltered with chromatography (2351 6m)
Analysis of or*aniccompound and anion
in biomaterials by 0/
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, -, .,,
.,,,,
',,,,
/,,,,IOIO33
--
A" %etermination of anions in a sample of straw
BrOBrO
33--
NONO22--
NONO
33--
8Min9
+! mM+! mM
/apillary /olumn:Analytes : Standard solution consists ofS(#
$4,I(#4,.r(#4,-($4,-!#4,I4,S/-4
/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM
3low Rate : #? with wa@elen*th ) B
Results and %iscussion
%etermination of Retention Time of Standard
Solution 3rom C*ure + the retention
time of each standardsolution
I(#4,.r(#4,-($
4,-(#4 )
respecti@ely : &"$D& D"&!!+!"F!& +$"'G&) minutes"
I(# more lie a mobile phase
compared to the other anions .efore Ctri et.al ha@e
studied the anion
3i*ure"+
C o m p a r i s o n / r e f e r
e n c e
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Separation of the standard solutionmixture
, ., ', /, 0,
1, m2bs
/apillary /olumn:Sampel : The Standard solution mixture of S(#
$4 +
,I(#4,.r(#4,-($4,-!#4,I4,S/-4
/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B $'&
+
$
#&
'
F
D
Results and %iscussion
3i*ure $"
The concentrations of the
standard solution mixturewas +! mM
3rom the resultchromato*ram was nownthe capillary columncapable for separatin*
mixtures" The compound anions
was separted respecti@elycontain of S(#
$4 +),I(#4
$),.r(#4#), -($
4&),-(#4
'),I4
F),S/-H
D) The compound number +is not separated perfect, itwould reuire furtherexperiment to Cnd@ariations that are suitable
for separatin* it"
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$ " ' & #
, ., ', /, 0,,
1,,,
-,,,,
/apillary /olumn:Sampel : Straw of rice/olumn : TS2 ;el I/4Anion4 5W60 +!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B$'&
# " G G +
' "D G G
+ ! " G # &
# # "
# ' !
Results and %iscussion
Aplication of the sample for in@esti*ations
of sample there are 'peas were detected"
It indicates there are
C@e separate
compounds in sample"
the retention time of
each compound
respecti@ely
$,'#"GG+',DGG+!,
G and ##,#G! )
Minutes
Sample were
extracted usin* aua r
Straw of rice
extracted #
a!a regia with a
concentration of /2 m7
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Straw ofrice
/apillary /olumn:Sampel : Standart solution ofS(#
$4,I(#4,.r(#4,-($4,-!#4,I4,S/-4
/olumn : TS2 ;el I/4Anion45W60 +!!mmx!,#$ I"% )Eluent : Sodium /hloride #! mM3low Rate : #? with wa@elen*th ) B $'&
, ., ', /, 0,
-
.
3
'
1
/
4
.1
3 ., m2bs
Mixin*standart
Results and %iscussion
/omparison sample with standardsolution
%rom 1 pea$s are detected of the
sample3 there are - pea$s of the
same retention time with the
standard sol!tion
So from the comparison it3 can
indicated that the compo!nds
contained in the sample straw
are I,-'3Br,-
'3 and +,-' anions8
%or 4 pea$s again not yet $nown
anions3 and need for f!rther
experiment to $now them8
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Standard solution
, ., ', /, 0, -,,
,
,51
-
,1
7
Results and %iscussion
, -, ., 3, ', 1, /,
,
,51
-,
17
." %etermination of cations in a sample of straw
Mixin* standardsolution
/apillary /olumn:Sampel : 0iK,-aK,2 K,M*$K,/a$K
/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM
3low Rate : #? with wa@elen*th ) B
0iK+)
-aK$)
2K#)
M*$K
/a$K
+
$
#
10 mM
%etermination of the retention time of
standard solution
The concentrationsof mix standardsolution was +! mM
3rom the result
chromato*ram wasnown the capillarycolumn capable forseparatin* mixtures"
The retention timesM* and /a cationsare @ery lon*compared with othercations so they arenot used as astandard solution
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NH4+
, ., ', /, 0,
,
,5.
,5'
,5/
,50
-,17
Results and %iscussion
Add +*59
Before add +*59
Separation of the standard solutionmixture
Addin* the ammonium
into the standard
solution mixture can be
nown with the ad@ent
of pea after sodium
cation
/apillary /olumn:Sampel : 0iK,-aK,2 K,M*$K,/a$K
/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B
$+!
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Straw of rice
, , ., 3, ', 1, /,,
,5.
,5'
,5/
,50
,17
Results and %iscussion
A
Extracted #
A8 *Cl / 7
B8 A!aregia /2 m7
B
Application of sample with diLerentextractors
ea!
Aua re*ia as extraction
the straw is better as
compared to hydro*en
chloride
The results showed a
pea in the
chromato*ram of the
straw sample extraction
usin* auare*ia
"#era not
ea!
/apillary /olumn:Sampel : 0iK,-aK,2 K,M*$K,/a$K
/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B$+!
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, ., ', /, 0, -,,,
,5.
,5'
,5/
,50
-,1
7
Results and %iscussion
/omparison sample with standard solution
The result chromato*ram
from comparison standard
solution with sample not
there suitable so that the
cation species contained in
the sample not nown
/apillary /olumn:Sampel : 0iK,-aK,2 K,M*$K,/a$K
/olumn : I/42ation4 SW +!mmx!,#$ I"% )Eluent : /upper Sulfat +! mM3low Rate : #? with wa@elen*th ) B
$+!
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A" /onclusion
+" Straw of rice containin* anion I(#4, .r(#4 and-(# 4
$" Auare*ia is as extraction suitable for ricestraw
." Su**etion+" 3urther experiment is needed to determine
anions and cations in straw of rice$" the next experiment is needed modiCcations
to the mobile phase, extract solution, and theaddition of a standard solution as comparison
/onclusion And Su**etion
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