CD11a/Integrin alpha-LPolyclonal ANTIBODYCatalog Number:15574-1-AP

For Research Use Only

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Basic Information Catalog Number:15574-1-APSize:28 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG7920

GenBank Accession Number:BC008777GeneID (NCBI):3683Full Name:integrin, alpha L (antigen CD11A (p180),lymphocyte function-associated antigen 1;alpha polypeptide)Calculated MW:129 kDaObserved MW:

Recommended Dilutions:IHC 1:50-1:500

Applications Tested Applications:IHC, ELISASpecies Specif icity:humanNote: suggested an gen retrieval with TEbuffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0

Positive Controls:IHC : human tonsillitis tissue;

Background Information

Notable Publications Author Pubmed ID Journal Application

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

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Selected Validation Data

Immunohistochemistry of paraff in-embeddedhuman tonsillitis tissue slide using 15574-1-AP(CD11a/Integrin alpha-L Antibody) at dilution of1:200 (under 10x lens). heat mediated antigenretrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraff in-embeddedhuman tonsillitis tissue slide using 15574-1-AP(CD11a/Integrin alpha-L Antibody) at dilution of1:200 (under 40x lens). heat mediated antigenretrieved with Tris-EDTA buffer(pH9).