New value added productsNew value added productsfrom rest raw material.from rest raw material.Protein hydrolysatesProtein hydrolysates
andand LipidsLipids
SINTEF Fisheries and Aquaculture 1
SINTEF Fisheries and AquacultureSINTEF Fisheries and Aquaculture
Aquaculture Technology
Fisheries Technology
Fish Processing Technology
Marine Resource
Technology
International projects and consultancy
The Norwegian Fisheries and Aquaculture Simulation Centre
Aquaculture Fishing Gear Fish Process Marine Juvenile EngineeringValsneset, Bjugn,
Norway
EngineeringFlume tank Hirtshals, Denmark
EngineeringProcess Hotel ,
SeaLab Trondhem Norway
Process Engineering
SeaLab Trondheim Norway
Norwegian Infrastructure
Roadmap
SINTEF, NTNU Others SINTEF SINTEF SINTEF , NTNU
The European Fisheries and Aquaculture Engineering
ESFRI
EATP
SINTEF Fisheries and Aquaculture 2
The European Fisheries and Aquaculture Engineering Laboratories
~43 000 t lipids
~6 500 t w:3 lipids
Rest rawmateial ~ 26 million people per year
290 000 t
58 000 t t i~58 000 t proteins
~ 2,5 million people per year
SINTEF Fisheries and Aquaculture
Marine lipidsMarine lipids
Marine lipids are rich in long chain ω-3 polyunsaturated fatty acids(LC-PUFA), esp. eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)
“essential”, RDI (EPA + DHA) ~ 250 mg/day EPA (20:5, n-3)essential , RDI (EPA DHA) 250 mg/daybeneficial effect on human health
I t f W t E th i l i t k f f tt fi h d fi hIn most of Western-Europe there is a low intake of fatty fish and fishproducts - intake of EPA and DHA is far below nutritionalrecommendations.
A way to increase the populations intake of3 FA i t dd fi h il t diff t f d d t
DHA (22:6, n-3)
w-3 FA is to add fish oil to different food products– ”functional foods”
SINTEF Fisheries and Aquaculture
Omega 3: beneficial health effectsOmega 3: beneficial health effects
Omega 3 fatty acids (EPA and DHA) have positive effectOmega 3 fatty acids (EPA and DHA) have positive effect on human health and developmentBrain and nerves contained high amounts and DHA (40Brain and nerves contained high amounts and DHA (40-60% LCPUFA of total lipids)
Brain, eyesight development, mental condition , y g p ,
Prostaglandins control inflammation processes. EPA influences formation of prostaglandins and relieve illness progress
Arthritis, arteriosclerosis
EPA and DHA can be defined as “essential” nutritional components
SINTEF Fisheries and Aquaculture
Marine oils from fresh raw materials
Fresh Norwegian rest raw materials for high quality omega-3 oil productionp
Rest raw materials from salmon and trout aquacultureRest raw materials from pelagic filleting industryLiver from cod or other white fish species (both wild and farmed)Liver from cod or other white fish species (both wild and farmed)Oil from other marine sources (calanus, krill etc.)
SINTEF Fisheries and Aquaculture
Fatty acidFarmed Atlantic
Salmon TunaJack
mackerel HerringAtlantic cod liver
Atlantic menhaden
South American anchovy Capelin Sardine Seal Krill
C14:0 4.2 3.9 7.3 7 3.3 7.3 7.5 7 8 5.0 9.5
C16:0 15.7 17.6 15.7 16 13.4 19 17.5 10 18 11.3 20.8
C 16:1n-7 5.1 5.4 5.1 6 9.6 9.1 9 10 10 14.3 9.9
C18:0 4.2 4.1 3.1 2.7 4.2 41.1 0.9
C18:1n-9 16.5 12.4 9.9 13 23.4 13.2 11.6 14 11 22.310.5
C18:1n-7 3.5 2.4 2.9 4.910.3
C20:1n-9 3.3 1.3 8.3 12 7.8 2 1.6 17 4 7.0 <1
C22:1 n11 2 5 0 5 5 8 20 5 3 0 6 1 2 14 3 2.3 <0.5C22:1 n11 2.5 0.5 5.8 20 5.3 0.6 1.2 14 3
C18:2 n-6 (Ln)* 6.6 1.9 1.7 1.3 1.2
1.1 2.3
C18:3 n-3 (LnL)*(LnL)
C18:4 n-3
C20:5 n-3 (EPA)* 7 1 12 4 10 9 5 11 5 11 17 8 18
6.6 18.2(EPA)* 7.1 12.4 10.9 5 11.5 11 17 8 18
C22:6 n-3 (DHA)* 15.7 27.8 11.5 6 12.6 12.6 8.8 6 9
8.7 9.5
Total n-3 19 7 27 7
SINTEF Fisheries and Aquaculture
LC-PUFA 26.7 41.9 24.4 11 25.7 22 27.4 14 27 19.7 27.7
”Long-chain omega-3 speciality oils”, Harald Breivik, 2007, The Oily Press, Bridgewater, England
Structure of SINTEFs rest raw material l tfplatform
Marine rest raw materials
Ingredients
Lipid platform Hydrolysate platform
Consumables,pharmaceuticalsplatform pharmaceuticals
Aim: To produce oil and protein hydrolysate of premium quality
SINTEF Fisheries and Aquaculture 8
SINTEFs lipid platformSINTEFs lipid platform
PurificationProduction of
ExtractionProduction of
StabilisationPrevent oxidation in
TransformationProduction ofProduction of
highly purified oilProduction of
raw oilPrevent oxidation in
oil and foodProduction of
new lipids
R t E i t fExtraction equipmentin lab and pilot scale.
From 50 ml batcht
Reactors (100L) for:Degumming, bleachingDeodorization (1000L)Short path distillation
Reactors:6 reactors of 250 ml32 reactors of 3 LSeveral reactorsf 8 L t 1500L
Equipment for measuring oxidation
kinetics (OSI, and
ti )to1000 kg/h continuous
(Mobile SeaLab)
Short path distillation(lab and pilot 10l/h)
Infrastructure(0,5 mill €)
from 8 L to 1500L oxygen consumption).Effect of
pro oxidants andanti oxidants
Analytical capabilities:Characterisation: Fatty acid composition, lipid classes, phospholipid classes, positioning of fatty acids
Wet chemical: PV Ansidin value TBARS
SINTEF Fisheries and Aquaculture 9
Wet chemical: PV, Ansidin value, TBARS,Instrumentation: GC, Iatroscan, HPLC-Corna, NMR (Low-high field), GC-MS-MS, LC-TOF-MC
Oil and proteins from rest raw material (1 and 2)
Mincer Heat exchange
Polishing centrifuge
Trikanter
Stick water
Dry material Crude oil 1
Mincer Heat
exchange Hydrolysis
tank
Tricanter Polishing sentrifuge
H d D Oil ftHydrolysate
Dry material
Oil after hydrolysis
SINTEF Fisheries and Aquaculture 13
Oil and proteins from rest raw material (3)
HeatMincer Heat exchanger
Stick
Polishingcentrifuge
Tricanter
Dry Crude oilStick water
ymaterial
Crude oil2
Hydrolysistank
Polishingcentrifuge
Tricanter
Stick water hydrolysate
Dry material
centrifuge
Dry material Oil after stick water
hydrolysis 3
SINTEF Fisheries and Aquaculture 14
y y
Ultra fresh herring oil qualityUltra fresh herring oil quality
F h i l C ti l Oil fFresh raw material Conventionalfish oil
Oil from silage
Prosess1 Prosess 2 Prosess 3
Oil 1 Oil 2 Oil 1’ Oil 3
FFA (%) 0,12 0,30 0,14 0,13 3-5 8-10
PV 1,9 3,3 2,3 2,4
AV 0 8 4 9 0 3 6 5AV 0,8 4,9 0,3 6,5
Totox 4,6 11,5 4,9 11,3 15-25 20-25
SINTEF Fisheries and Aquaculture 15
SINTEFs Hydrolysis platformSINTEFs Hydrolysis platform
Processti i ti
Biochemistryf h d l i
Processd i
Processifi tioptimizationof hydrolysis designverification
Choice of enzyme(s)Characterisation of
raw material and productReactors,
Selection of conditionsfor best profitYield, qualityTemperature,
The best hydrolysisconditions are tested
on site withMobile SeaLab.
Scale up data .Dewatering and drying
in cooperation with SINTEF EnergyReactors,
6 of 250 ml, 32 of 3L
Temperature, water content
pH, time…32 reactors of 3L
Mobile SeaLab.1 reactor of 800L.Capasity 500kg/h
SINTEF Energyand
Enginering companies
Analytical capabilities:Characterisation: Protein, lipid, ash and water content, Amino acid composition, free and bound,
Molecular weight distribution, Water holding capasity, Foaming and emulsifying capability, Fat absorption Antioxidative properties
SINTEF Fisheries and Aquaculture 17
Fat absorption, Antioxidative properties.Instrumentation: GC, FPLC, NMR (Low-high field), GC-MS-MS, LC-TOF-MC, electrophoresis
Fish protein hydrolysates (FPH)
Antioxidative properties Tolerate heat without precipitating
Bulk propertiesBioactive properties Nutritional propertiesHigh nutritional valueAntioxidative properties
AntihypertensiveAntithrombicImmunomodulatory activities
Tolerate heat without precipitating Soluble over a wide range of pH Water holding capacity Foaming and emulsifying properties
High nutritional value
Easy digestible proteins/peptides
Amino acid compositionAnticoagulant and antiplateletproperties
Accelerate calcium absorbtionPossess hormone-like peptides
properties Absorb and retain/hold oil Gel forming ability Cryoprotective properties Protect proteins on drying
SINTEF Fisheries and Aquaculture
and growth factorsObesity modulation
Protect proteins on drying
Bitterness of the FPH from herringBitterness of the FPH from herring7,0
6,0
Bitterness
4,0
5,0
es le
vel
e ess
3,0
lativ
e bi
ttern
e
2,0
Re
0,0
1,0
SINTEF Fisheries and Aquaculture 19
Pa+Br Endogenous Flavourzyme Bromelain Protamex Papain Promod Alcalase
Reduction of oxidation (oxygen uptake rate (OUR) -effect of pH
80
90
, %4.55.56 5
effect of pH
6.5 +80
%
4.55 4
Fe3+ HaemoglobinpH
60
70
80
xida
tion 6.5
60
datio
n, %
5.46.5
30
40
50
on o
f ox
40
on o
f oxi
d
liposomes(lipid concentration 6mg/mL)
10
20
30
Inhi
bitio 4.5 -
20in
hibi
tio
0FPH20 FPH50 C CK P Norland
protein 1 25mg/mL
0FPH50 FPH20 C CK P Norland
protein, 4mg/mLprotein, 1.25mg/mLProtein concentration
1.25mg/mL 4 mg/mL
SINTEF Fisheries and Aquaculture
Model food system for evaluation of effect f dd d fi h t iof added fish proteins
C i SS
SINTEF Fisheries and Aquaculture
Concentration test Storage testSensory test
25,0
Primary oxidation
15,0
20,0q/
kg o
il Aroma
Control
FPH 50
Primary oxidation products – Peroxide
value
5,0
10,0
PV m
eq FPH 50
Norland HFC
MariPep
0,00 1 2 3 4 5 6 7
Storage time, months 5,0
S d3,0
3,5
4,0
4,5
es/g
oil
MariPep
FPH 50Secondary oxidation products
TBARS1 0
1,5
2,0
2,5TB
ARS
um
ole FPH 50
Norland HFC
Aroma
Control
0,0
0,5
1,0
0 1 2 3 4 5 6 7
Storage time months
SINTEF Fisheries and Aquaculture
Storage time, months
Concluding remarksConcluding remarksNorway has huge amounts of available herring rest raw
t i l f d ti fmaterial for production of:Ultra fresh marine oils Herring has a high concentration of cetolic acid C22:1 n11Herring has a high concentration of cetolic acid, C22:1, n11. Functional and bioactive peptides/proteins
Ultra fresh marine oils will have higher price in the market
Peptides/proteins from fish rest raw materials can be used for human consumptionp
FPH show antioxidative activity which is beneficial in
SINTEF Fisheries and Aquaculture
FPH show antioxidative activity which is beneficial in industrial product formulations
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