Definitions
Volume: the space a substance occupies.
Graduated cylinders: cylindrical vessel that can house a higher volume.
Volumetric flasks: glassware used to measure a specific volume.
Burettes: long tubes with a stopper (valve).
Pipettes: apparatus that allows liquid to be drawn.
Pipette-aids: devices used to assist the pipette to draw the liquid.
Micropipetting: devices used to measure and draw smaller volumes.
Meniscus: curvature on the surface of a liquid in confined spaces. Measure at the base of the meniscus.
Pipette Rules
Rules:
1. Never submerge your pipette in liquids.
2. Never go beyond the maximum or minimum volume.
3. Never share pipettes that are designated to your station .
4. Never try to repair your pipette, ask help from a lab manager or PI.
5. Never invert your pipette with liquids in a tip.
Tip Volumes
Single & Multi Channel Pipette TipsP20:2µL-20µLP100:10µL-100µLP200:20µL-200µLP1000:100µL–1000µLP50 Multi: 5µL-50µL P100 Multi: 50µL–100µL P100 Multi: 50µL-300µL
Serological Pipette Tips1mL- 50mLsVolume designated by color as show at the top of the serological tips.
Pipetting Web Links
How to Use a Pipette Pipette Techniques
Pipette User Guide
Measuring Volume With Glassware
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