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Genomics Proteomics Core
Facility
PROTEOcore
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ORGANISATION PROTEOcore
ORGANISATION PROTEOcore
Cell culture
Transgenic animal
model
DNA Sequencing
Post-genomic
Histology, cytology,
morphology
Imaging
Glassware & Lab
maintenance
Microarray
laboratory
ProteomicsLaboratory
Bioinformatics &
Biostatistics
High throughput
analytical chemistry
Translational
research
System
biology
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Research & Co-operationResearch & Co-operation
Alliance of local members of the cluster bycompetition and supply relationships orcommon interests
examples at ILS:
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RESEARCH TOPICS
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Research TopicsResearch Topics
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omics
Transcriptomics
High throughput screening
Moleculebank
Genomics / proteomics
Sclerosis Multiplex, POCD,
Alzheimer
Oral antidiabetics
Safetypharmacology
ChemiotherapyNanotechnology
Proteomic platform
Microbial genomics /proteomics / transcriptomics
Fermentation/Bioprotect
DNS animal breeding
Molecular farming
Safety pharmacology
Microarray platformBiomedical
measurement
Agro-biotechnology
Metabolomics
Enriched egg
Technology Transfer ManagementBiomedical Informatics
Joint technology platforms
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Proteomics GenomicsEven more different
Gene/ RNA
dynamic
Protein
dynamic
Stable molecules
Handling cheap/ easy
Minimal modification
Works in isolation
Fragile molecules
Handling dependent
Labile modification
Protein-interaction
Localization dependent
Handle
Tech
HTP
Sequencing (established) MS related (not yet)
DNA array / genotyping/expression / CGH/
Protein Chip (not yet)
Antibodies array (not yet)
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PROTEOcore Research &Co-operation
PROTEOcore Research &Co-operation
Indication areas at ILS
Diseases of the Central Nervous System;
Inflammatory Diseases; Food Allergens
Cancer; Biomarkers (proteins, peptides)
Proteomics (food proteins and bioactive peptides)
Probiotics, Functional Food.
Indication areas are based on: Regional resources,know how and research co-
operations within the local institutes and companies
Global market expectations
Focus increases the efficiency of Innovation
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J Assouline, Genome-wide measurement of gene expression
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MIAME - cADNmicroarray
MIAME - cADNmicroarray
6STEPS of a Microarray Experiment
Array
Array design
Hybridization protocol
Statistical analysis
Data mining
Image acquisition and
quantification Database building
Filtering
Normalization
HybridizationSample
(S. Sansone)
MIAME Sample QC Sampletreatment
Metadata
mARNs
Extraction protocol
QC Target
preparation
(labeling) - QC
QC
QC
QC
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PROTEOMICS
Sample preparation
1st dimension (1D)
Gel-based Gel free
2nd dimension (2D)
Detection of protein spots Imaging analysis & 2D gel databases
Spot handling: excision, in gel digestion
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MIAPE-GE
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Proteomics
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Need of standards in Proteomics
HUPO-PSI Organization Standard data formats
MIAPEs
PEFF: A Common Sequence DatabaseFormat in Proteomics
PRIDE
Standard data format converters
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2011 dissemination
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Future visionFuture vision
Continue work on important data analysis problems in
bioinformatics and language technology
Applications, including fielded and commercialized ones
Theory and method development
Collaboration across units, disciplines, industry
Future emphasis on
Mining rich public biological databases
Discovery of patterns in complex irregular structures,
discovery of similarities and analogies
Methods forsemantic analysis of large text collections
language and domain-independence, efficiency
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The FUTURE RESEARCH
POLICY Enhance volunteer laboratory work
(undergraduates, graduates master and
PhD students, postdocs)
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USAMV Rector supports
Doru PAMFIL, PhD an accomplished professor of
genetics, he has expertise in the management of manynational and international projects and veteran of large,public research institutions.
Throughout his career he has focused onexpanding undergraduate research opportunitiesand improving the education and professionalexperience of graduate students, with a focus on
underrepresented groups.
Pamfils academic work focuses on Biotechnology(marker assisted selection, detection of GMOs,genetic transformation, micropropagation,microbiology). The GMOs Laboratory CERTOMG -was included in EUROPEANNETWORK OF GMOLABORATORIES (ENGL) in 2009
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"The problem solving, systems thinking, and teamwork aspects of biotechnology can benefit all students,whether or not they ever pursue an scientific career," said Doru PAMFIL, Rector of the University of
Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, head of INSTITUTE OF LIFE SCIENCES(LIS).
"An biotechnology education and science curricula that does not include at least some exposure tobiotechnology is a lost opportunity for students and for the nation."
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Helping Hands for AddressingHelping Hands for AddressingExcellence in Research, EducationExcellence in Research, Education
and Training Needs forand Training Needs for
BiotechnologyBiotechnology
For more information on
future partnerships, contactDr. Ramona SUHAROSCHI
Email: [email protected]
Tel/fax:+40 264 425575
Calea Floresti 64
400509, Cluj-Napoca
For more information on
future partnerships, contactDr. Ramona SUHAROSCHI
Email: [email protected]
Tel/fax:+40 264 425575
Calea Floresti 64
400509, Cluj-Napoca