CURRICULUM VITAE (CV)
Personal Details:
Name & Surname Khaled AlTaweel
Academic Degree PhD in Functional Genomics &Genetic Engineering
Place & Date of Birth Damascus, Syria, 1/1/1972Sex MalePresent Position & Address Research Associate in the Department of
Plant Science, Faculty of Agricultural & FoodSciences University of Manitoba, Winnipeg,MB R3T2N2.Tel : (204) 474-8352 (O) : (204) 417-0613 (H)Fax : (204) 474-7528E-mail : [email protected]
Academic and Technical Education:
Year Degree Average% Institution
2010/2011 Research Associate in the field of Functional Genomics -
Manitoba University, Facultyof Agricultural & Food
Sciences, Canada
2009/2010
Post-Doc in the field of Functional Genomics -
Manitoba University, Facultyof Agricultural & Food
Sciences, Canada
2004/2007
Ph. D in the field of Genetic Engendering A+
Osaka Prefecture University,Graduate School of Life &
Environmental Sciences, Japan2001/200
3M. Sc
in the field of Biotechnology 90 Faculty of Agriculture,Damascus University, Syria.
1995/1996
Diploma in the field of Crops 76.16 Faculty of Agriculture,
Damascus University, Syria.1989/199
4B. Sc
in Agricultural Engineering 61.17 Faculty of Agriculture,Damascus University, Syria.
MSc. Thesis Title: In Vitro Microtuberization of Draja and Some Introduced Varieties ofPotato using Plant Tissue Culture Techniques.
PhD. Thesis Title: A Bacterial Transgene for Catalase Protects Translation of D1 Proteinduring Exposure of Salt-Stressed Tobacco Leaves to Strong Light.
Post-Doc Project: - Molecular Identification and Characterization of Wheat Resistance Genes Expressed
under Different Isolates of Fusarium Graminearum.Research Associate Project (continuous):
- Transcriptome Profiling of Wheat Differentially Expressed Genes under DifferentIsolates of Fusarium Graminearum
Techniques Known:
1) Plant tissue culture & Plant breeding (conventional and molecular)2) Molecular characterization of plant germplasm3) DNA markers for crop improvement (RAPD, AFLP, SSR, SRAP,SCAR marker)4) Preparation of transformation constructs (vectors)5) Molecular characterization of transformants6) Plant genetic transformation & GMOs detection and evaluation.7) Plasmid DNA isolation, quantification, and restriction analysis.8) Genomic DNA isolation, quantification, and restriction analysis.9) Primer designing, PCR, RT-PCR, Gel electrophoresis, Geldocumentation, ELISA, Southern hybridization, Northern & Westernblotting.
10) Differential genes expression methods: Differential Display RT-PCR(DDRT-PCR), Suppressive Subtractive Hybridization (SSH).
11) Library construction: cDNA library synthesis, subtracted cDNA library.
Research Experiences:
1) Standardization plant tissue culture protocol2) Regeneration survey (evaluation of different plant genotypes forresponse to tissue culture)3) Bacteria culturing, selection of transformed cells, maintenance ofcultures.4) Performing DNA markers (DNA isolation, restriction enzymes,results scoring, phylodiversity tree)5) Plasmid DNA isolation, and transformation of bacteria forpreparation of transformation construct (vectors) 6) Biolistic (Bombardment) method of plant genetic transformation7) Molecular characterization of putative transgenic plants throughmolecular biology techniques (e.g., PCR, qRT-PCR, Southernhybridization, ELISA.8) Field evaluation of transgenic plants for agronomically importanttraits.9) GMOs detection by PCR-based methods & protein-based methods. 10) Transcriptome profiling and gene expression analysis.11) cDNA library construction. 12) Subtracted cDNA library construction.
Professional Training Courses:
Course Title Sponsors Date PlaceTissue Culture of Potato General Organization for Seeds
Multiplication (GOSM) 2002 Aleppo, Syria
Introductory GeneManipulation for Agriculture
Japan International CooperationAgency (JICA)/Japan
Aug. 14th – Dec. 13th,2002 Osaka, Japan
DNA Molecular MarkerTechniques for Crop
Improvement
The International Center forAgricultural Research in The
Dry Areas (ICARDA(
Sept. 7th – 18th,2003
ICARDA,Aleppo, Syria
DNA Molecular MarkerTechniques for Crop
Improvement
ICARDA, GCSAR, GEF &UNDP Project
Mar. 14th – 21st,2004
GCSARDamascus, Syria
Genetic Transformation andDNA Marker Applications for
Crop Improvement
German Agency for TechnicalCooperation (GTZ)
Mar. 2nd – 13th,2008
ICARDA,Aleppo, Syria
Participation in Congresses, Conferences & Workshops:
Title Conference/Workshop Organizers Date Place
Conference of Biotechnology 41st Science Week Supreme Council ofSciences.
Nov. 3rd – 5th, 2001 Damascus, Syria
Biosafety in AgriculturalBiotechnology in Syria. Workshop GCSAR / FAO &
ICARDA
Aug.30th – Sep.1st,200303 Damascus, Syria
Environmental Stress Inhibitsthe Repair of PhotodamagedPhotosystem II by Suppressingthe Transcription andTranslation of D1 ProteinEncoded by psbA Gene in
Tomato, (Presentation.(
Annual KansaiSymposium
Osaka PrefectureUniversity
July 22nd,2006 Osaka Prefecture
University, Japan
Translational Machinery ofpsbA Gene is Protected inTransgenic Tobacco PlantsExpressing E. coli Catalase(KatE) in Chloroplasts,
(Presentation.(
Annual Meeting
Japan Society forBioscience,
BiotechnologyAgrochemistry
(JSBBA(
Mar. 24th – 27th,2007
Tokyo Universityof Agriculture,
Japan
A Bacterial Transgene forCatalase EnhancesPhotosynthesis ProteinsTolerance againstEnvironmental Stress,(Presentation).
6 th Scientific
Conference
General Commissionfor Scientific
Agricultural Research(GCSAR(
Aug. 26th – 27th,2008
GCSAR, DoumaDamascus, Syria
Agrobacterium-MediatedTransformation of TomatoExpressing Bacterial Catalasein Chloroplasts,(Presentation).
1st International
Conference of
Agricultural
Science
Aleppo Uni., Syria&
Musel Uni., Iraq
Oct. 13th – 15th,2008
Faculty ofAgriculture,Aleppo Uni.,
Syria
Abiotic Stress Resistance inTobacco is Enhanced byOverexpression of A CatalaseEnzyme, (Presentation).
Annual MeetingCanadian
PhytopathologicalSociety (CPS)
June 22nd – 25th,2009
University ofManitoba,Manitoba,
CanadaA survey to examine a SCARmarker as a tool todifferentiate resistance andsusceptible cultivars and linesof canola against differentLeptosphaeria maculans PG3isolates, (Poster).
Annual Meeting CanadianPhytopathological
Society (CPS)
June 22nd – 25th,2009
Delta HotelWinnipeg, MB,
Canada
6th Canadian Workshopon Fusarium Head Blight
(Attendance)Workshop
Agriculture and Agri-Food Canada
(AAFC)
Nov. 1st – 4th,2009
Marriott Hotel,Ottawa, Ontario
CanadaMolecular Identification andCharacterization of WheatResistance Genes Expressedunder Different Isolates ofFusarium Graminearum,
4th Annual MeetingCanadian-GermanCollaboration onPlant Genomics
Research
Nov. 5th – 6th,2009
Agriculture andAgri-Food
Canada(AAFC/ECORC), Ottawa, Canada
(Presentation). (GABI-Canada)
Construction of subtractivecDNA library andidentification of differentiallyexpressed genes from wheatcv. Sumai-3 under Fusariumgraminearum infection,(Poster).
Joint AnnualAnnual Meetingand Conference
CanadianPhytopathological
Society (CPS)with the
Pacific Division ofthe American
PhytopathologicalSociety
June 20th – 23rd,2010
University ofBritish
Columbia,Vancouver, BC,
Canada
Differentially expressed genesof wheat cultivar Sumai3resistant to the pathogenicfungus Fusariumgraminearum, (Poster).
9th InternationalMycological
CongressInternationalMycologicalAssociation
August 1st – 6th,2010
EdinburghInternationalConferenceCentre, UK
Application of SuppressionSubtractive Hybridization(SSH) to CloningDifferentially ExpressedcDNA in wheat (Sumai3)Under Fusariumgraminearum Infection(Poster)
2010 NationalFusarium HeadBlight Forum
U.S. Wheat andBarley Scab Initiative
(USWBSI)
December 7th-9th , 2010
Hyatt RegencyMilwaukee,Milwaukee,
Wisconsin, USA
Induction of wheat defensegenes in response to Fusariumgraminearum infection,(Presentation).
Manitoba RegionalMeeting
The CanadianPhytopathological
Society (CPS)
December 9th,2010
CRC-Agriculture& Agri-Food
Canada,Winnipeg, MB.
Transcriptome Analysis of awheat cultivar infected bydifferent chemotypes ofFusarium graminearum(Poster)
Joint AnnualMeeting andConference
The AmericanPhytopathological
Society (APS)&
The InternationalAssociation for the
Plant ProtectionSciences (IPPC)
August 6th -10th 2011
Honolulu,Hawaii, USA
Transcriptome Profiling ofDifferentially ExpressedGenes of Wheat after Infectionwith Different F.graminearum Chemotypes(Presentation).
7th CanadianWorkshop
on Fusarium HeadBlight
http://www.cwfhb.org/Program%20-
%20CWFHB%207.pdf
Agriculture and Agri-Food Canada
(AAFC)
Nov. 27th
– 30th, 2011
Delta HotelWinnipeg, MB,
Canada
Are wheat genes expresseddifferentially when infected bydifferent F. graminearum Chemotypes, (Presentation).
Manitoba RegionalMeeting
http://phytopath.ca/newsletter/news_v56-1.pdf
The CanadianPhytopathological
Society (CPS)
December 8th,2011
CRC-Agriculture& Agri-Food
Canada,Winnipeg, MB.
Comparative gene expressionanalysis of susceptible andresistant Wheat infected byFusarium graminearum.(Presentation).
Annual Meeting
The CanadianPhytopathological
Society (CPS)With the International
Plum Pox Virus
June 24th-27th,2012
Marriott Hotel,Niagara Falls,
Ontario, Canada
List of Publications
Khaled Al-Taweel, Al-Maarri Khaleel, Kheeti Mamoun, Abdul-Kader Ahmad (2004)Effects of Some Factors Influencing on in vitro Tuberization of Potato cv. “Draga”.Damascus University Journal for the Agricultural Sciences 20: 265-280
Khaled Al-Taweel, Toshio Iwaki, Yukinori Yabuta, Shigeru Shigeoka, Norio Murata,and Akira Wadano (2007) A Bacterial Transgene for Catalase Protects Translation ofD1 Protein during Exposure of Salt-Stressed Tobacco Leaves to Strong Light. PlantPhysiology 145: 258-265
Toshio Iwaki, Kazunori Shiota, Khaled Al-Taweel, Daisuke Kobayashi, AtsushiKobayashi, Kensaku Suzuki, Toshifumi Yui, Akira Wadano (2008) Inhibition ofRuBisCO Cloned from Thermosynechococcus vulcanus and Expressed inEsherichia coli with Compounds Predicted by Molecular Operation Environment(MOE). J. Bioscinece Bioengineering 105: 26-33
Khaled Al-Taweel, Dilantha Fernando, Anita Brule-Babel (2011) Construction andCharacterization of a cDNA Library from Wheat Infected with Fusariumgraminearum Fg 2. International Journal of Molecular Sciences 12: 613-626
Khaled Al-Taweel, Dilantha Fernando (2011) Differential Gene Expression is aPromising Tool for Understanding Host–Pathogen Interactions. In: Al-Mughrabi K(Ed) Plant science and biotechnology in North America: Focus on Canada II. TheAmericas Journal of Plant Science and Biotechnology 5 (Special Issue 1), 1-10.
Abstracts in Journals
Al-Taweel, K., Chen, X., Parks, P., Ghanbarnia, K., Dusabenyagasani, M., andFernando, W.G.D. (2009) A survey to examine a SCAR marker as a tool todifferentiate resistance and susceptible cultivars and lines of canola against differentLeptosphaeria maculans PG3 isolates. Canadian J Plant Pathology 31: 475 – 504
Al-Taweel, K., Fernando, W.G.D., and Wadano, A. (2009) Abiotic stress resistance intobacco is enhanced over expression of a catalase enzyme. Canadian J PlantPathology 31: 475 – 504
Al-Taweel, K., Guo, X., Fernando, W.G.D., and Brûlé-Babel, A. (2010) Construction ofsubtractive cDNA library and identification of differentially expressed genes fromwheat cv. Sumai-3 under Fusarium graminearum infection. Canadian J PlantPathology. In press
Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2010) Induction of wheatdefense genes in response to Fusarium graminearum infection. Canadian J PlantPathology. In press.
Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Transcriptome Analysisof a wheat cultivar infected by different chemotypes of Fusarium graminearum. TheAmerican Phytopathological Society (APS) & The International Association for thePlant Protection Sciences (IPPC). Honolulu, Hawaii, USA.
Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Transcriptome profilingof differentially expressed genes of wheat after infection with different Fusariumgraminearum chemotypes.7th Canadian Workshop on Fusarium Head Blight.Winnipeg, MB, Canada. . "In press”.
Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Are wheat genesexpressed differentially when infected by different F.graminearum Chemotypes? Canadian J Plant Pathology. 56: 6.
Papers in Symposia proceedings at international/national meetings:
Al-Taweel, K., Brule-Babel, A.L., and Fernando, W.G.D. 2009. Molecular Identificationand Characterization of Wheat Resistance Genes Expressed under Different Isolatesof Fusarium Graminearum , 3rd Annual Canadian-German Collaboration on PlantGenomics Research, (GABI-Canada), Agriculture and Agri-Food Canada(AAFC/ECORC), Ottawa, Canada, Nov. 5th – 6th,