Isolation and Characterization of E. coli from Day Old Chicks of a Selected Breeder Farm in Bangladesh
There are 3867000 breeders in 227 hatcheries in Bangladesh1
Bacterial diseases caused highest mortality in chicken2
Very few attempts were taken to study the E. coli of day old
chick( DOC)3,4
E. coli of DOC is important both for DOC and the parent stock.
Objectives
Study design: cross-sectional
Duration: April 2008 to May 2009
Study site: laboratory of the Department of Microbiology and Hygiene,
Bangladesh Agricultural University,Mymensingh
Visceral sample collected aseptically from eighteen DOC of different
groups were collected from a Breeder farm of Valuka, Mymensingh
Tested the incubated sample for E.coli through competitive cultural
examination and biochemical tests
Total Viable Count (TVC) was done on nutrient agar following ten-fold
dilution
Lastly antibiotic sensitivity test was conducted to know their drug
sensitivity and resistance pattern.
1. Anonym, 2010. Farm Poultry And Livestock Survey. Bangladesh
Bureau of Statistics, Statistics Division, Ministry of Planning,
Government of the People’s Republic of Bangladesh.
2. Uddin MZ, Samad MA , Kabir SML, 2011. Mortality and disease
status in hy-line and isa-brown strains of layer chickens reared in cage
system in Bangladesh. Bangl J Vet Med, 9, 1 –16.
3. Islam A, Majumder S, Rahman A, Trisha AA, Amin R, 2012.
Retrospective study of commercial poultry diseases. Eurasian J. Vet.
Sci. 28, 2, 116-121.
4. Islam A, Trisha AA, Das M, Amin MR, 2009. Retrospective study of
some poultry diseases at Guibandha district in Bangladesh. Bangl J Vet
Med, 7, 239-247.
M.A. Rashid1, 2, K.A. Chowdhury1, S.M.S. Mahmud1, T. Chowdhury3, Abdulla Al Mamun Bhuyan4, Zannat – Nahar1, S.K.Paul1,3*
Background Results
Conclusions and Recommendations
References
• From the findings of different cultural, staining, biochemical
examinations it may be concluded that the isolated organism was E. coli
The load of E. coli was higher among the lower grade chicks and in gut
The E. coli we isolated during this study showed resistance to certain
antibiotics which tends to recommend the proper use of antibiotics
In future more extensive studies can be conducted to design a
successful control estrategias
We were able to isolate E. coli was from gut, liver and lungs of DOC
(Table 1).
Total viable count (TVC) in gut of “A” grade chicks was 2.3×106, in “B”
grade 2.6×106, in “C” grade 2.9×106, mean of which was 2.6×106
(Table 2).
The TVC of liver in “A” grade chicks was 2.6×105, in “B” grade
2.7×105, in “C” grade 2.8×105, the mean of which was 2.6×105 (Table
2).
in the lungs of “A” grade chicks was 2.2×104, in “B” grade 2.3×104 and
in “C” grade was 2.5×104. The mean of which was 2.3×104 (Table 2).
Antibiotic sensitivity test revealed that E. coli were sensitive to
Ciprofloxacin, Amoxicillin and Enrofloxacin, but resistant to
Erythromycin and Cloxacillin.
Methods
To determine the E.coli in vital organs like liver, lungs and gut of
healthy (A grade), weak (B grade), culled (C grade) chicks obtained
immediately after hatching
To determine the Total Viable Count of bacteria to show the
difference in their density in different organs of the three grade of
chicks
To determine the antibiotic sensitivity pattern of isolates
Acknowledgements I am grateful to Department of Microbiology and Hygiene, Faculty of
Veterinary Science, Bangladesh Agricultural University ,Mymensingh
-2202, Bangladesh. Special thanks to Dr.Najmul haider ,Dr.Ausraful
islam and Dr. Sukanta (RI of icddr,b) for their valuable suggestion to
preparing my poster.
1Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh2Department of Livestock Services,3International Centre for Diarrheal Disease Research (icddr,b), Dhaka, Bangladesh,4Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Bangladesh
A” quality chicks (Grade-A)
B” quality chicks (Grade-B)
C” quality chicks (Grade- c)
Correspondence: [email protected]
Batch No. Grade No of chicks Sample type E.Coli present
Batch 1
A 2 Gut Present
B 2 Gut Present
C 2 GutLiverLung
Present
Batch 2
A 2 Gut Present
B 2 GutLiver
Present
C 2 GutLiverLung
Present
Batch 3
A 2 Gut Present
B 2 GutLiver
Present
C 2 GutLiverLung
Present
Table 1. Bacteria isolated from different organs of 3 batches of DOC
Table 2. Total viable count (TVC) obtained from gut of DOC
Sample type Grade TVC (CFU/ml) Mean ±SD
Gut
A 2.3×106
2.6×106
B 2.6×106
C 2.9×106
Liver
A 2.6×105
2.7×105
B 2.7×105
C 2.8×105
Lung
A 2.2×104
2.3×104
B 2.3×104
C 2.5×104
Test Result
Reaction with Dextrose
Maltose
Lactose
Sucrose
Mannitol
All are +
Indole +
MR +
VP -
Table 3. Biochemical characteristics of E. coli
+ indicates Positive, MR = Methyl Red, - indicates Negative, VP= Voges-Proskauer
E. coli growth on EMB agar media-Metallic shin
E. coli growth on SS agar
E.Coli growth on Blood agar
Antibiotic sensitivity pattern
Total viable count (TVC) of E. coli
CIP
AMOXENRO
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