IRRI’s Collaborative Rice Research Activities in Kenya
Rosemary Murori, ScientistIRRI - ESA
1. Evaluation and selection of new breeding lines
2. On farm demonstrations
3. Sharing of best performing lines with NARES to facilitate their breeding programs
4. Characterization
5. Seed production training
6. Small scale farm machinery fabrication
7. Rice Knowledge Bank
8. Pathology studies
Results
Recommendations
Activities
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Evaluation and selection of new breeding lines and on Farm Demos
ON -40 GSR Lines -33- RBW-Uganda
AYT- 13 MET 2012 for stability and adaptability of target environment.
-IRRI-KARI Kibos joint demonstration
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3. Sharing of best performing lines with NARES
3. Characterization of Local Germplasm
Neighbour-Joining tree of simple matching dissimilarity coefficient using SSR
Dendrogram using all genotypes included in study
Judy Joachim, Msc
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5. Seed production Training
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6. Small scale farm machinery fabrication
Hydro-Tiller testing at Mwea
KARI (Kenya) Rice knowledge Bank
8. Pathology studies
Characterization of Pyricularia oryzae population in irrigated ecosystem in Kenya
By:Benson Ouma Nyongesa, Joseph Bigirimana, Beatrice A.
Were, Rosemary Murori
NIB and Collaborators
• Rice blast caused by P. oryzae is one of the major constraints to rice production in irrigated ecology in Kenya.
• Understanding the diversity of blast population in target areas
• Is essential for rice breeding programs focused on disease resistance.
• Objectives: Characterize diversity of blast population and identify genes effective against blast population in irrigated ecosystem in Kenya.
Rice blast disease in a farmers’ fields at the coastal region
MATERIALS AND METHODS
• Sites: Mwea, West Kano and TARDA-Garsen.
• 51 lines, (NILs & monogenic lines ) and 2 parents (CO39 & LTH genetic and 4 local varieties (BW 96, Basmati 270, I|TA 310).
• Basmati 370 was used as susceptible check
• Design: Row-Column design with 3 reps.
• Scoring stage: 60 DAT using 0-5 scale rating system.
RESULTSMean squares for leaf blast lesion type across experimental sites in 2013.
Source of Variation DF SS MS Pr>F
Row 7 0.1303 0.0186*** <.0001
Column 6 0.1372 0.0.0229*** <.0001
Rep 2 0.0011 0.0005 0.8625
Site 2 1.5277 0.7638*** <.0001
Variety 55 3.4732 0.0631*** <.0001
Site*Variety 110 2.1892 0.0199*** <.0001
Error 307 1.1431 0.0036
General Mean 0.5289
R-Square 0.8671
CV (%) 11.2831
Root MSE 0.0597
***: Significant at P≤ 0.01
Reaction patterns of 51 differential rice lines to natural blast population at experimental sites in 2013.
Sites R MR S HS
Mwea 2 15 29 5
West Kano 2 8 36 5
TARDA-Garsen 27 5 15 4
Key: R = resistant (scale: 0-2); MR = moderately resistant (scale = 3); S = susceptible (scale = 4); HS = highly susceptible (scale: 5)
Sites % lines/varieties overcome by blast
Mwea 66
West Kano 80
TARDA-Garsen 36
Lines/varieties attacked by blast population at different sites in 2013.
• Significant interaction between sites and lines, indicating that P. oryzae population and pressure differed in all the three sites was observed
• Pathogen diversity:West ano (80%)>Mwea (66%)> TARDA-Garsen (36%)
• Incidence of predominant lesion type: West Kano (90%)> TARDA-Garsen (82%)> Mwea (34%).
Table 4. Differential lines carrying effective genes at different sites in 2013
Sites Resistant genesModerately Resistance genes
Mwea Pish and Pi9Pik-s [IRBLks-S], Piz, Piz-t, Piz5
West Kano Piz-t and Pik Piz-5, Pish, and Pi9
TARDA-Garsen Pik-s from Shin2], Pik, Piz-t, Pik-h, Pik-p, Pi7(t), Pib
Piz5, Pish, Pi1, Pi9, Pik-m, Pi20 and Pita-2
Reaction patterns of differential lines to natural blast population at Mwea in 2013.
Plate 3. Reaction patterns of differential lines to natural blast population at West Kano in 2013.
Reaction patterns of lines to natural blast population at TARDA-Garsen. Line 28 (Pita-2), Line 45 (Pik-p) in 2013.
• High diversity of P.oryzae population was observed across the sites.
• Disease pressure: West Kano> TARDA-Garsen>Mwea.
• Re-evaluation of identified blast R genes in 2014.
• Collection of blast pathogens races particularly from East Africa to be used along side others in the development of rice differential system.
CONCLUSION/RECOMMENDATION
ACKNOWLEDGEMENT
•KARI, •MIAD,•NIB-(Ahero/West Kano & TARDA
Thanks
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