Introduction1048673microscopy
Introduction1048673microscopy
1048673most cells are 01-1 mm1048673unaided eye sees 1mm
1048673Hooke1048673built microscope (30X)
Purpose of Microscope
1048673magnification1048673 enlarged image by a factor
1048673resolving power1048673 ability to separate two adjacent points in a
specimen1048673contrast
1048673 how well details stand out against thebackground1048673parfocal
1048673remain in focus when switching objectives
Rules and Care of theMicroscope abridged
1048673carry microscope correctly1048673clean lenses with lens paper and slides
with Kimwipes1048673set smallest objective in place (4X) and
bring stage close of objectives1048673use oil with oil immersion lens (100X) only
1048673clean all lenses when finished1048673have instructor check scope before storing
bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X
bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Purpose of Microscope
1048673magnification1048673 enlarged image by a factor
1048673resolving power1048673 ability to separate two adjacent points in a
specimen1048673contrast
1048673 how well details stand out against thebackground1048673parfocal
1048673remain in focus when switching objectives
Rules and Care of theMicroscope abridged
1048673carry microscope correctly1048673clean lenses with lens paper and slides
with Kimwipes1048673set smallest objective in place (4X) and
bring stage close of objectives1048673use oil with oil immersion lens (100X) only
1048673clean all lenses when finished1048673have instructor check scope before storing
bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X
bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Rules and Care of theMicroscope abridged
1048673carry microscope correctly1048673clean lenses with lens paper and slides
with Kimwipes1048673set smallest objective in place (4X) and
bring stage close of objectives1048673use oil with oil immersion lens (100X) only
1048673clean all lenses when finished1048673have instructor check scope before storing
bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X
bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X
bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Other Microscopes
bull 1048673dissectinggreater working distancereflected and transmitted light
bull 1048673light microscope
bull 1048673phase contrast microscope
bull 1048673transmission electron microscope TEM
bull 1048673scanning electron microscope SEM
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Cell Theory
bull Cell Theory
bull 1048673all organisms are made of cells
bull 1048673the cell is the smallest living thing
bull 1048673all cells arise from pre-existing cells
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Eukaryotic Cells
bull Plant Elodea cells w water
bull draw and label 400X total mag
bull Label nucleus plasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Chloroplastvacuole
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Eukaryotic Cells
bull Plant Onion bulb cellsw iodine
bull 1048673draw and label 400X total mag
bull Label nucleus w DNAplasma membrane
bull cytoplasm w cytosolcell wall of cellulose
bull Nucleolusvacuole
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Eukaryotic Cells
bull Animal human cheek cells
bull w methylene blue stain
bull draw and label 400X total mag
bull nucleus w DNA plasma membranecytoplasm w cytosol
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
Prokaryotic Cells
bull 1048673label cellcell wall of peptidoglycan
bull plasma membranecytoplasm w cytosol
bull Ribosomenucleoid w DNA
bull draw three basic shapes
bull determine stain reaction
bull Gram positive = purple
bull Gram negative = red
ModelsStudy both animal and plant cell
modelsbull 1048673
ModelsStudy both animal and plant cell
modelsbull 1048673
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