IntroductionBiochem I
Biol 3252 / Chem 3251
Sept. 7, 2006
Course details• See course webpage for overview:
flash.lakeheadu.ca/~dlaw/3251.html
• Full lecture schedule posted online, will try to keep on track
• Office CB4018, Office hours Thurs. 3-4 PM, or email me to make an appointment ([email protected])
Welcome!Welcome!
Lecture topic summary • More detailed list online
Sept. 7-12 General intro and intro to metabolism
Sept. 14-26 Lipids
Sept. 28 Test 1: Metabolism and lipids
Oct. 3-19 Carbohydrates
Oct. 24 Test 2: Carbohydrates
Oct. 26-Nov. 9 Nucleic acids
Nov. 14 Test 3: Nucleic acids
Nov. 16-28 Proteins
Nov. 30 Makeup lecture and/or review
Course details• Lectures:
– Tues and Thurs– Sept. 7 to Nov. 29, 2006, 1:00 PM - 2:30 PM
UC2011 (a/k/a Upper Lecture Theatre–new location!)
• Labs: – CB2050 and 2051– Sept 11 to Nov 28– Section F1: Monday 2:30P-5:30P– Section F2: Tuesday 2:30P-5:30P
• Read your lakeheadu.ca email regularly to receive class updates
Marking scheme• Three term tests worth 15% each (1 h each, written in
class)– Sept 28– Oct 24– Nov 14
• Final in Dec. worth 35%• Lab worth 20% (4 labs @ 5% each)
– Lab coordinator: Jarrett Sylvestre ([email protected]) – TA: Chris Edmunds ([email protected]) – Lab check-in next week (Sept 11 & 12)– You need a lab coat, safety glasses and a lab manual to
participate in the labs • safety apparauts available in bookstore • lab manual is a PDF on the course website, available early next
week for download)
Textbook• (also required for Bchem II)• “Biochemistry”, 6th ed., Berg et
al. (2006).• Available in bookstore• The textbook has a multimedia
site, where you have access to– interactive exercises – animated 3D tutorials – learning tools – See course website for URL
Amazon.ca: $ 186.79
Note that the 5th edn text was used last year and is still useable
Course overview• In this course, we will talk about the four
major types of macromolecules and their subunits, synthesis and degradation, and functions
• These macromolecules occur in particular places in the cell and fulfill specific functions
• Bchm II next term will integrate these concepts into a holistic discussion of some aspects of their metabolism (enzymes, signal transduction, disease)
What is biochemistry?Life seems to be a paradox!“Living things are composed of lifeless
molecules”— Albert Lehninger, 1982.• Biochemistry allows us to use chemical and
physical rules governing individual molecules to predict behaviour of living organisms
• Living matter uniquely: – is highly organized (macroscopically,
microscopically)– is made of components with distinct functions (from
limbs to fatty acids)– can extract, transform, use energy from environment
(fight entropy!)– can self-replicate
What separates non-living and living?• 18th century
(Enlightenment): vitalism, a/k/a “the philosophick mercury” sought by alchemists
• Now seek testable, repeatable experiments to explain natural phenomena (scientific method)
• Chemical molecules inside and outside living organisms act according to physical laws
• As part of metabolism, biomolecules also act as part of the “molecular logic of the living state”
universe-review.ca
Core biochemical concepts
1. Biochemical unity2. The importance of water for life:
interaction with other molecules3. Acids and bases: how molecules
interact chemically4. Buffers and pH: controlling the
environment in the cell (homeostasis)5. The interdependence of biochemical
pathways
Core concept 1: Biochemical unity• Biomolecules: so different,
so identical• 107 species x 50,000
proteins per species = ~1011 proteins! All are different but– Many are similar
(homologous) in sequence and function between species (e.g., enzymes such as pyruvate kinase)
– They are all made up of the same building blocks: 20 amino acids
• From immense simplicity comes immense diversity!
Multiple sequence alignment of pyruvate kinase proteins from different kingdoms
http://www.bio.davidson.edu/courses/Molbio/MolStudents/spring2000/deeb/macdnasis.html
Biochemical unity suggests a common ancestor• Extends to other macromolecules as well (DNA,
RNA, lipids, CHO) and their monomers: identical or extremely similar between species
• Has phylogenetic implications: what constitutes a species?– “Species” traditionally defined in Linnean systematics
as similar looking (also, can interbreed, etc.)– Though macromolecules of inheritance and function
are homologous between species, between individuals within a species (and even within families) they are very close to identical
All these organisms have DNA, RNA, proteins constructed the same way!
Alberts et al., Molecular biology of the cell, 4th edn.
Common ancestry reflected in macromolecule sequences
• Evident when constructing phylogenetic trees relating macromolecule (e.g., DNA) sequence to species origins
• Usually for one highly conserved gene (e.g., 18S ribosomal RNA gene)
Fig. 1.3
• Biochemical unity is reduced the older the split between species but core similarities remain predominant
• Tree of life is being reformatted based on biochemical information!
Domain(or Kingdom)
Genus
Related genuses/ families/ phyla
close
distant
Common ancestor
Life = using energy for useful work• Harness energy from metabolism
to do useful work (Gibbs free energy, ΔGo’)
• Common energy currency in all living organisms
• Living organisms not in equilibrium with environment: fight entropy, “selfishly” store energy in anabolism by making useful molecules
Useful energy
Dissipated energy
• Energy is conserved & stored through the formation of chemical bonds: the chemistry of molecules important to life
• Let’s walk through a brief review of chemical bonds of importance in biochemistry
Fig. 1.3, Lehninger, Principles of biochemistry (1982).
Chemical bond typesCovalent
• Formed by sharing outer valence shell electrons to fill shell– One shared e- pair = 1 (single) bond– Two shared pairs = double bond, etc.
• We can talk about bond energy – C-C is 348 kJ/mol
e.g., nucleophilic attack forms new covalent bond between molecules
• Chemical reactions break and form covalent bonds
These can form in biological molecules such as purine bases
– Strong bonds result if e- can form resonance structures, e.g., benzene (C6H6; C=C is 186 kJ/mol); carbonyl (C=O ~730 kJ/mol)
Uncredited structural formulas are from text, 5 th ed.
p. 7 of text
Noncovalent bonds• depend on dipole-dipole interactions• recall that a dipole is an object whose centers of
positive and negative charge do not coincide • this occurs because many molecules possess
electron-rich and –poor regions• these regions can interact and result in
noncovalent bonding between molecules – e.g., HCl: electrically neutral but possesses a dipole
H – Cl
– e.g., H2O
Noncovalent chemical bond typesElectrostatic• Dependent on electrical charges between noncovalently
bound atoms• According to Coulomb’s Law:
E = k q1 q2 / r2
• a/k/a ionic bonds: a chemical bond in which one atom loses an electron to form a positive ion and the other atom gains an electron to form a negative ion
• Example: NaCl composed of two ions: Na+ (e- donor) and Cl- (e- acceptor)
Closer = higher energy
Forms tightly packed lattice
p. 7 of text
http://cwx.prenhall.com/bookbind/pubbooks/hillchem3/medialib/media_portfolio/text_images/CH02/FG02_09.JPG
Noncovalent chemical bond typesHydrogen• Relatively weak (4-12 kJ/mol) but are crucial for three-
dimensional structure of biomolecules (NAs, protein)• Of primary importance in biochemistry: water can H-bond
with itself or can easily interact with other molecules: “universal solvent”
Hydrogen donor
Hydrogen acceptor Water swaps its
H-bonds with those between other molecules
Example: H-bonds between complementary bases in DNA
http://www.biosci.ohiou.edu/introbioslab/Bios170/170_8/at.html
Noncovalent chemical bond typesVan der Waals• A force acting between
nonbonded atoms or molecules • Relatively weak, even smaller than
hydrogen bonds (2-4 kJ/mol)• Based on the fact that charge
distribution around atoms is dynamic (e- cloud constantly in motion)
• Differences in one atom’s charge distribution complementarily perturb neighboring distributions
• These asymmetries attract• Example: attraction between
phenyl groups in neighboring phenylalanine residues in a protein
• Play major role in protein folding
Fig 1.10
attractive from a distance…
but repellent close up
Things to remember about noncovalent bonds
• More than one type of noncovalent interaction can occur at once
• Though individual bond strengths are small, summed over entire biomolecules they are chemically (and biologically!) significant
proteinhttp://www.nature.com/emboj/journal/v19/n4/full/7592187a.html
DNA
Core concept 2: Water is essential for life• Weak, noncovalent interactions seem less important than
covalent bonds but are crucial biochemically• Examples:
– Substrate-enzyme– Hormone-receptor– Protein-protein
• In biochemistry, all these rely on the properties of water– Polar: asymmetrically distributed charge: bent molecule!– H-bonds hold water together cohesively– Universal solvent: disrupts and weakens electrostatic interactions,
forms solvent shells around ions, dissolves essential polar molecules so that they can move/diffuse inside cell
• Presents a problem for crucial interactions between polar molecules
• Hydrophobic microenvironments present in the cell• So many molecules to dissolve, so little water: water is
limiting inside the cell
Enzyme
Substrateattracted to
via one or combination of noncovalent interactions
Image: http://www.chemistry.wustl.edu/~edudev/LabTutorials/Carboxypeptidase/carboxypeptidase.html
Core concept 3: acids and bases• Water dissolves compounds• These are often charged in solution
– pH-dependent – Depends on shape & atoms in molecule: many are non-planar– Can become
• protonated = basic (e.g., ammonia: NH3)
• deprotonated = acidic (e.g., acetate: CH3COOH)
• Thus an acid is a proton donor, and a base is a proton acceptor
acid
conjugateacid
conjugatebase
base
Image: http://www.windows.ucar.edu/tour/link=/physical_science/chemistry/ammonia.html
Core concept 3: acids and bases• Water itself dissociates into ions:• We can quantify how readily this dissociation happens by
calculating a dissociation constant (Keq):
• Water is mostly nonionized: Keq at 25°C is 10-14
• We can also calculate Keq for other species too, like acids
• Recall that pH is a measure of the H+ concentration of a solution:
• We can similarly define pK for an acid:
= Conc products
Conc reactantsLarge + : mostly ionsLarge -: mostly undissociated
, so
Core concept 3: acids and bases• pH and pK are related (Henderson-Hasselblach
equation):
• If an acid HA is half dissociated to H+ and A-, then [A-] = [HA]
• Thus, pK is the pH at which half of the acid is dissociated
• This is important when considering the buffering capacity of these molecules in solution, which leads us to…
can be rearranged to give
Then = 1, log = 0, and pH = pK.
Add more OH-, pH does not change as radically
Minimum change in centre of curve where [A-] = [HA]
This is the pK of acetic acid!
Core concept 4: buffers and cellular pH• Acid-base conjugate pairs (e.g., acetic acid and acetate)
in solution resist changes in the solution’s pH: they are buffers
• Consider adding base (OH-) to acetic acid (HA):
• Remember titration curves from analytical chem? – Titrate OH- into a solution of acetic acid to force its dissociation:
SIGMOIDAL RESPONSE!
Initially, small changes in [OH-] produce large changes in pH
1
1
2
2
3
3
Stryer, “Biochemistry”, 3rd edn. (1988)
Core concept 4: buffers and pHWhy are we learning chemistry again
(what does this mean biochemically?)• Weak acids are effective at buffering their
environment at pH values near their pK• pK values indicate at what pH a functional group
is ionized (titrated) a proton from a molecule– It can take a small amount of OH- (at low pH):
e.g., COOH COO- (pH 2.3)– Or a high amount of OH- (at high pH):
e.g., NH3+ NH2 (pH 9.6)
Increasing pH
Stryer, “Biochemistry”, 3rd edn. (1988)
Core concept 4: buffers and pH• Protein amino acid sidechains have different R (functional)
groups, each with their own pK• Proteins are more or less ionizable depending on
– pH of environment– Types of R groups they contain
• This affects – how they interact with other proteins – activity – solubility
• Natural (in vivo) buffers include the bicarbonate ion• Cells must control their interior pH (of their intracellular
space, or c________)• Proteins, enzymes, have optimal pH for their activity• The core concepts we covered are crucial for
understanding how molecules are made and interact (e.g., for the rest of this course!)
• Though the course will present metabolism of the four macromolecule types as largely distinct, all metabolic pathways are linked
– Share products
– Macromolecules combine to change their function, e.g.,
• Proteins decorated with lipids, carbohydrates
• Complexes of nucleic acids and proteins
• This will be addressed in greater detail when we discuss the integration of metabolism
And
finally! Core concept 5: the interdependence of biochemical pathways
Fig 15.2
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