Industrial Strength Sunscreen

Then… 1920’s

• Sunburns were just a part of life.

• Any product available was claiming to make your

skin Brown.

1930’s

• Tanning is now fashionable.

• The first sun protection products are on the

market.

• Sunburns still a problem.

1940’s

• A New Concept “Tanning without burning”!!!

• Sunburns continue to be an issue.

• More products out to soothe a sunburn rather

than prevent.

1950’s

• Getting a better tan quickly is important.

• First self tanning products emerge.

• Sun lamps advertised.

• Sunburns, sunburns, sunburns!!!

Now….

1960’s

• Finally Tanning without burning.

• Still the desire for a “better tan”.

• Sprays start to become popular.

• Sunburns unstoppable.

1970’s

• Tanning still popular but the first links found between the sun

and skin aging.

• SPF makes its day beau.

1980’s

• First water resistant products.

• Tanning still popular but less emphasis.

1990’s

• A lot of mixed information and tanning remains popular.

• Education about skin cancer becomes prevalent.

• Fake tan products are produced.

2000’s

• Greater emphasis on sun protection.

• Importance of UVA protection. Skin Cancer Kills more

people annually than any

other cancer.

• UV radiation is part of the light spectrum that reaches the earth from the sun. It has wavelengths shorter than visible light, making it invisible to the naked eye. These wavelengths are

classified as UVA, UVB, or UVC.

• UVC is absorbed by the ozone layer and does not reach the earth.

• Both UVA and UVB, however, penetrate the atmosphere and play an important role in conditions such as premature skin aging, eye damage (including cataracts),

and skin cancers. UVA & UVB Rays can weaken your immune system making it difficult to fight off Skin Cancers.

Light Spectrum

UVB Rays causes skin reddening and sunburn, tends to damage the skin’s more superficial epidermal top layers, in other words a SUNBURN.

UVB = A SUNBURN It plays a key role in the development

of skin cancer, darkening of the skin, and photo aging (wrinkles & crow feet).

The most significant amount of UVB Sun Rays hits the earth between the hours of 10 AM and 4 PM.

UVB rays can burn and damage your skin both in the summer and winter.

UVB Rays do not significantly penetrate glass, while UVA Rays penetrate vehicle glass windows. Truckers call this a “left handed tan”.

Sun Damaged Skin Cells

UVA rays account for up to 95% of the UVA radiation that reaches the Earth surface will penetrate the epidermal layer of your skin.

UVA Rays are strong enough to bounce off the surface of concrete, water and snow causing skin damage, UVB Rays can not.

UVC = Weak Sun Ray UVB = Moderate Sun Ray UVA = Strongest Sun Ray

• Cells that are damaged by continual UVA

exposure can’t repair themselves fast enough leading to the development of skin cancer.

2 Types of UVA wavelengths – Short & Long

and are accountable for 35% of skin cancers. Short wave UVA is 5 times more

cancerous Long wave UVA has oxygen free

radicals.

85% of UVA rays passes through glass.

• A skin growth that increases in

size and appears pearly,

translucent or multicolored.

• A spot or sore that does not heal

within three weeks.

• A mole, birthmark, beauty mark or

any brown spot that:

• Changes color

• Increases in size or thickness

• Changes texture

• Has an irregular outline

• Is bigger than 6mm or ¼ inch

(Size of a pencil eraser)

Unexpected spots

• Skin cancer can occur even in

spots that are not usually

exposed to sun. Some of those

areas are:

• Scalp

• Soles of feet

• Palms

• Fingernails or toenails

• Back of legs

• Upper and inner thigh area

Basal Cell Carcinoma

• This is the most common form of skin cancer,

affecting over a million Americans every year.

Seldom spreads to other parts of the body but

this form of skin cancer can be extremely

damaging and disfiguring.

What it looks like?

• A reddish patch or irritated skin.

• Shiny bump that is pearly or translucent.

• An elevated pink growth.

• Sometimes even looking like a scar but with

poorly defined borders.

Treatment

• The procedure is called Curettage or

Electrodessication.

• Scraping away most of the affected area and

then removing the remaining bad cells with

a hot cauterizing pen.

• Literally cutting the cells out with the aid of

a local anesthetic.

NON-MELANOMA CANCER

Basal Cell Carcinoma

Do you know someone

who has skin cancer?

Squamous Cell Carcinoma • More than 250,000 new cases diagnosed every

year. SCC may occur on any part of the body but

are most common in areas frequently exposed to

the sun, such as the rim of the ear, lower lip,

face, bald scalp, neck, hands, arms and legs.

What it looks like?

• A scaly red patch with irregular borders.

• Sometimes looking like a wart or an elevated

bumpy part of the skin.

Treatment

• The procedure is called Curettage or

Electrodessication.

• Scraping away most of the affected area and

then removing the remaining bad cells with

a hot cauterizing pen.

• Literally cutting the cells out with the use of

a local anesthetic.

NON-MELANOMA CANCER

Do you know someone

who has skin cancer?

Melanoma • The most serious form of skin cancer is

Melanoma. Over 9,000 people die every year from

the disease. A cancerous growth that invades the

skin and can potentially grow deep into the skin

if not treated quickly.

• The biggest problem with Melanoma is if it is left

untreated it can metastasize and spread to the

lymph nodes. From there it can transport all

around the body – most commonly effected is

the brain, liver and bones.

What it looks like?

• Usually a brown or black color.

• Can arise from a pre-existing mole so look for

the following:

• Asymmetry of a mole.

• Border irregularity.

• Abnormally large in size, roughly the size of

a pencil eraser is much to large for a regular

mole.

• Spots or moles on your skin that seem to be

changing in size, shape or color.

Treatment

• Melanoma must be surgically

removed. If caught early just

the outermost layer of skin is

removed and produces

almost a 100% cure rate.

• If left untreated the

melanoma can reach deeper

to the blood vessels or

lymphatic channel and

spread throughout the whole

body.

• Avoid Tanning Beds at all costs! – Just 6 uses at a tanning bed increases your chance of getting skin cancer

by 73%.

• Use a Zinc Oxide Sunscreen whenever you Shellac your nails in a salon. • Examine your skin head-to-toe every month. Pay attention to your body, the

earlier an infection is treated the less harm it will cause.

• See your dermatologist or physician every year for a professional skin exam.

• Cover up with UV protective clothing, wear broad-brimmed hats, and UV-blocking sunglasses.

• Use a Zinc Oxide Sunscreen with an SPF of 15 or higher.

• DO YOU KNOW SOMEONE WHO HAS OR HAD SKIN CANCER?

General Facts

• Each year there are more new cases of skin cancer than

the combined incidence of cancers of the breast,

prostate, lung and colon.

• Treatment of nonmelanoma skin cancers increased by

nearly 77 percent between 1992 and 2006.

• Over the past three decades, more people have had skin

cancer than all other cancers combined.

• One in five Americans will develop skin cancer in the

course of a lifetime.

• Between 40 and 50 percent of Americans who live to age

65 will have either BCC or SCC at least once.

• Actinic keratosis is the most common precancer; it

affects more than 58 million Americans.

• Approximately 65 percent of all squamous cell

carcinomas and 36 percent of all basal cell carcinomas

arise in lesions that previously were diagnosed as actinic

keratoses.

• About 90 percent of nonmelanoma skin cancers are

associated with exposure to ultraviolet (UV) radiation

from the sun.

Melanoma and Non-melanoma

• The overall 5-year survival rate for patients whose

melanoma is detected early, before the tumor has

spread to regional lymph nodes or other organs, is

about 98 percent in the US. The survival rate falls to

62 percent when the disease reaches the lymph

nodes, and 15 percent when the disease

metastasizes to distant organs.

• One person dies of melanoma every hour (every 57

minutes).

• Melanoma is the most common form of cancer for

young adults 25-29 years old and the second most

common form of cancer for young people 15-29

years old.

• A person’s risk for melanoma doubles if he or she

has had more than five sunburns.

• One or more blistering sunburns in childhood or

adolescence more than double a person’s chances of

developing melanoma later in life.

Melanoma and Non-melanoma

continued…

• In adults 65 or older, melanoma treatment

costs total about $249 million annually. About

40 percent of the annual cost for melanoma

goes to treating stage IV (advanced) cancers,

though they account for only three percent of

melanomas.

• The estimated cost of treating melanoma in

2010 was $2.36 billion.

• The number of nonmelanoma skin cancers in

the Medicare population went up an average

of 4.2 percent every year between 1992 and

2006.

• In 2004, the total direct cost associated with

the treatment for nonmelanoma skin cancer

was $1.4 billion.

Tanning

• More than 170,000 cases of non-melanoma skin cancer in the

US each year are associated with indoor tanning.

• One indoor UV tanning session increases users’ risk of

developing squamous cell carcinoma by 67 percent and basal

cell carcinoma by 29 percent.

• The risk of basal cell carcinoma is increased by 73 percent if

one tans six times per year.

• Indoor tanners have a 69 percent increased risk of early-onset

basal cell carcinoma.

• Approximately 25 percent of early-onset basal cell carcinomas

could be avoided if individuals have never tanned indoors.

• More than 90 percent of the visible changes commonly

attributed to skin aging are caused by the sun.

• Daily sunscreen use by adults under age 55 can reduce skin

aging.

• People who use sunscreen daily show 24 percent less skin

aging than those who do not use sunscreen daily.

• Contrary to popular belief, 80 percent of a person’s lifetime

sun exposure is not acquired before age 18; only about 23

percent of lifetime exposure occurs by age 18.

• The sun protection factor of a sunscreen is a laboratory measure of the effectiveness of sunscreen — the higher the SPF, the more protection a sunscreen offers against UVB (the ultraviolet radiation that causes Sunburn).

• You simply have to multiply the SPF number X 10 to get the projected time frame before burning. This is only an estimate created by the sunscreen manufacturers.

SPF 15 = 2 ½ Hours (UVB Protection) SPF 30 = 5 Hours (UVB Protection)

• The difference in protection between an

SPF 30 and SPF 50 is 1%.

• The Skin Cancer Foundation maintains that SPFs of 15 or higher are necessary for adequate protection

80.00%

82.00%

84.00%

86.00%

88.00%

90.00%

92.00%

94.00%

96.00%

98.00%

100.00%

5 10 15 20 25 30 35 40 45 50 55 60 65

% o

f P

rote

ctio

n

SPF

SPF vs UVB Protection

SPF 30 SPF 50+

SPF 20

SPF 15

ISO 24442 – International Standards Org. Sunscreen Standard for determining UVA

Protection, tested on human skin.

ISO 24443 – International Standards Org. Sunscreen Standard for determining UVA

Protection, machine test.

ISO 24444 – International Standards Org. Sunscreen Standard for determining SPF (Sun

Protection Factor).

FDA – New Monograph for Sunscreen Standards including – Full Broad Spectrum and

Water Resistance up to 80 minutes.

Canada

FDA & ISO 24444 EU

ISO 24444/3/2

Russia

ISO 24444/3/2

Korea

FDA &

ISO 24444/3

Japan

FDA &

ISO 24444/3

Australia/New Zealand

ISO 24444

India

ISO 24444

USA

FDA

South America

FDA

• Doctor Oz said he recommended using physical sunscreen with active ingredients like zinc oxide.

• Physical sunscreen does not pose a health danger because this type of sunscreen is not absorbed into the skin.

• Chemical sunscreen is the type that penetrates the skin.

In an article written by Arthur W. Perry, MD, FACS he wrote this about sunscreens… “To be effective, chemical sunscreens need to be rubbed into their skin 20 minutes before sun exposure. They do a pretty good job at blocking UV light, but they actually get used up as the sun shines on them. In fact, some sunscreens lose as much as 90% of their effectiveness in just an hour, so they

need to be reapplied often. This is not the case with zinc oxide and titanium

dioxide, the two mineral, or physical, sunscreens. These two work very differently – they sit on the surface of the skin and physically block UV light..”

There are two types of formulations used in manufacturing sunscreens:

Physical & Chemical.

Physical active ingredients create a barrier at the surface of your skin reflecting both the UVB & UVA Rays.

Chemical active ingredients require there be a chemical reaction within your skin in order to provide protection (Wait 20 minutes before going into the sun).

Zinc Oxide as shown in the diagram is the best physical protection for your skin.

Clear Zinc Oxide acts like tiny little mirrors reflecting the UVB & UVA Rays off your skin.

Clear Zinc Oxide is the best protection against UVB & UVA Rays.

Creates a physical barrier between your skin and the UVB & UVA Rays.

Do you know of someone

who has skin cancer?

Most ingredients only protect against UVB rays but it’s the UVA rays you need to protect against as well.

One of the most commonly used ingredient is Avobenzone, which begins to break down after 1 hour of sun exposure.

Some ingredients protect against just UVB rays and not against UVA rays. Others protect against both but not completely.

Only 1 ingredient protects against both UVB and UVA completely, CLEAR ZINC OXIDE.

ZINC OXIDE # 1

• No Sunscreen with

SPF higher than SPF 50.

• No more claiming “Water Proof” or “Sweat Proof”.

• Any sunscreen under

SPF 15 CAN NOT claim skin cancer protection.

• SPF 15 to SPF 50 can claim Skin Cancer prevention.

• There is no such thing as “all day protection”.

• “FDA states that it does not consider Sunscreen Wipes, Towelettes, Powders, Body Washes, and Shampoos currently eligible for review under the OTC monograph process.”

• If used as directed with other sun protection measures (see Directions) decreases the risk of skin cancer and early skin aging cause by the sun.

Spray Sunscreens

• Contains over 70% denatured alcohol a “Poison” used as a propellant. – Causes dizziness, headaches, watering of eyes,

irritation of respiratory tract and convulsions.

• Ingredients such as Avobenzone, Homosalate, Oxybenzone and Octisalate can cause respiratory irritation and difficulty breathing if “inhaled” through spray sunscreens.

KILLS golf course grass. So

what is it doing to your lungs?

Tattoos

UVA Ray damages tattoos as well by damaging the skin cells and causing the color to “fade”.

UVA Ray is the dominant ray and causes cumulative damage over time to the melanin, or pigment of the skin, also effecting skin cells colored by Tattooing.

Using a Zinc Oxide Sunscreen can prevent the UV Rays from destroying the color of a tattoo because it’s a physical block that reflects the UVA Rays before they fade the color of your tattoo.

Sun’s UVA Rays fade the

coloring of Tattoos

• In 1977 Bob Marley was

found to have malignant

melanoma in a football

wound on his right big toe.

• Marley refused treatment,

which at that time would be

amputation for fear it would

effect his dancing and his

Rastafarian belief that the

body is to be whole.

• Eventually the melanoma

spread to his brain, lung,

liver and stomach and he

died in 1981 at the age of 36.

• I have darker skin so I don’t need sunscreen.

– False, the darker your skin the

harder it is to tell if you are burning leading you to stay in the sun longer.

• If I put sunscreen on once a day it is enough.

– NO, FDA recommends you

should reapply every 2 hours. • Its cloudy out, I don’t need to

worry about wearing sunscreen.

– Wrong, up to 80% of the sun’s UV rays can pass through clouds.

• A little dab of sunscreen works just as well as a palm full.

— Minimum amount of

sunscreen needed to apply is 1 ounce (a shot glass full).

• The sunscreen I use says it is

Water Proof & Sweat Proof so I am protected right?

— Nothing is waterproof or

sweat proof. The FDA has denounced the ability to claim water proof and sweat proof.

• When is the sun damaging to skin? A. All day, every day? B. When you’re outside for more than an hour? C. If you burn? D. Only when it’s sunny?

• What is a UV index rating?

A. The amount of heat UV rays are expected to generate in a day? B. The time it takes UV rays to burn the skin? C. Amount of UV radiation expected to hit the earth? D. Amount of natural protection in your skin?

• The darker your skin the less damaging the sun? A. True? B. False?

• How long should one 8oz bottle of SPF 15 sunscreen last?

A. One weeklong outdoor vacation? B. A long weekend at the beach? C. An entire summer of weekends outside? D. One day at the beach?

• Which of the following about tanning beds is true? A. They help protect you by giving you a base tan? B. Can be damaging but much less so than lying in the sun? C. They are listed as a carcinogen by the U.S. Department of Health & Human Services. D. As long as they don’t deliver UVA rays they are safe?

• What’s the difference between UVB and UVA rays?

A. I don’t know. B. Both are damaging, UVA damages deeper layers of skin? C. UVA rays are damaging: UVB rays harmlessly cause you to burn? D. UVA rays aren’t damaging: UVB rays cause you to burn?

• If your makeup contains sunscreen do you need to

wear additional SPF on your face? A. Yes? B. No? C. Only if you’ll be outdoors for the entire day?

• What does it mean when a sunscreen is waterproof? A. No such thing as “Water Proof” B. You can get in and out of the water many times and still be protected? C. You can get in and out of water once and still be protected? D. You are protected for as long as you are in water?

Answers: A, C, B, B, C, B, A, A

• Industrial Sunscreen is in partnership with the Arizona Cancer Center’s Skin Cancer Institute.

• Sponsoring the “Protect Your Skin Program” where companies can purchase a gallon of Industrial Sunscreen, 2 dispensers, and 2 informational plaques seen here.

15% of sales goes back to Cancer

Research.

• Programs like these help to educate the public about how important sunscreen is in preventing skin cancer.

Skin Cancer Prevention

Partner

Elephants cover

themselves with a layer of

dust to bock UV Rays.

A Tiger finds rest in the

shade to get out of the

Sun.

Capybara covers itself in

thick mud preventing UV

Rays to penetrate its

thin skin.

Flamingo’s feathers act

like clothing to protect

them from the Sun’s Rays.

• Making Strides

Breast Cancer

Walk

• Relay for Life

• Phoenix 5K

• Hopekids

Walk

• Pat Tillman 5K

• Baja 500 Truck

Race

• Broad Spectrum 30+ Sunscreen formulated for the outside worker.

• Continual protection in the summer and winter.

• Formulated with Zinc Oxide, best physical protection. • Available in all sizes. New Airless Pump Technology. • Greaseless.

• Ultra High UVA Protection.

• Adheres to new FDA Monograph.

• Dermatologist Recommended.

• Made In the USA

Industrial Strength Sunscreen

A Safe Tan is

an Oxymoron.

“Bubba Tested”

For samples call Bubba 888-860-7424

Sun Protection by SPF Determination (FDA) – 80 Minute

Water Resistance

Definition:

FDA regulation and determination of the SPF (Sun Protection Factor)

pre-exposure and post exposure after immersion in water. This test is

on humans using 10 participants of varying skin pigmentation. Water

resistance test claims can be 0 minutes, 40 minutes, or 80 minutes. The

sunscreen has to meet the same SPF value as claimed on label before

and after immersion.

MED: Min Erythemal Dose (UV Light Exposure)

I: Intensity of light source ST: Static SPF (pre water immersion)

WR: Water Resistant SPF

(post water immersion)

Most important results are the last two because it demonstrates the SPF

factor before water immersion is 36.54 and after 80 minutes of water

immersion the SPF is 32.70. Retaining the SPF claim through water

immersion.

EVALUATION OF SUN PROTECTION

BY SPF DETERMINATION (FDA) -WATER RESISTANT - 80 MINUTE WATER IMMERSION

Table

Sponsor: R&R Lotion

AMA Lab No.: N-0479

Client No.: SUNSCREEN # 859 exp: 4-15

Subject Sex MED/ I Skin MED I MED II STD WR SPF Values

(7%PadO/

ID # Hr (Amps) Type J/M2 J/M2 3%Oxyb) Control Static WR

62 7072 F 126.9 5.7 I 30.33 30.33 16.30 15.00 39.60 34.50

82 9684 F 127.4 6.0 II 30.33 30.33 16.30 18.00 34.50 30.00

72 7921 F 128.4 6.0 I 30.33 30.33 16.30 15.00 39.60 34.50

72 2368 F 128.6 5.7 I 30.33 30.33 16.30 15.00 34.50 30.00

78 2670 F 128.8 6.7 I 30.33 30.33 18.75 15.00 34.50 34.50

78 8873 M 125.7 7.9 II 30.33 30.33 18.75 18.00 39.60 34.50

88 7551 F 127.4 5.8 II 60.89 60.89 16.30 15.00 34.50 30.00

70 5092 M 127.2 6.3 III 75.59 75.59 18.75 18.00 39.60 34.50

50 3379 F 126.3 6.0 II 46.20 46.20 18.75 18.00 34.50 34.50

56 2212 F 128.8 7.3 III 46.20 46.20 16.30 15.00 34.50 30.00

MEAN (x) 17.28 16.20 36.54 32.70

STANDARD DEV (s) 1.27 1.55 2.63 2.32

STD. ERROR 0.40 0.49 0.83 0.73

S.E. % OF MEAN 2.31 3.02 2.27 2.23

N 10 10 10 10

UPPER 5% t DIST. 2.2622 2.2622 1.8331 1.8331

A VALUES 0.9051 1.1082 1.5267 1.3471

LABEL SPF 16 15 35 31

ISO 24442 In-Vivo Determination of Sunscreen UVA Protection for

International Standards

Definition:

ISO stands for International Standards Organization mainly used in Europe. This test is In-Vitro,

meaning it requires human test subjects, and determines the UVA Protection Factor claims. 10

participants are used in this test and exposed to varying degrees of UV radiation.

EVALUATION OF UVA PROTECTION FACTOR (ISO 24442)

Table

Sponsor: R&R Lotion

AMA Lab No.: N-0479

Client No.: SUNSCREEN # 859 exp: 4-15

Expected UVA-PF: 10

No. Date Subject Sex Race Age ITAo Skin Lamp MW/ MPPDDu MW/ MPPDDu MW/ MPPDDp MW/ MPPDDp UVA-PFi UVA-PFi Tech.

cm2 I cm2 II cm2 S1 Std cm2 Product S1 Std Product

ID # Type No. Amps I (sec) Amps II (sec) Amps S1 (sec) Amps (sec) Initials

1 6/19/2013 66 6606 M H 45 26.7˚ II 4553 68.4/5.1 65 68.1/5.1 65 68.7/5.1 260 67.5/5.1 650 4.0 10.0

2 6/19/2013 48 3535 F H 54 26.1˚ III 4553 67.5/5.1 81 67.1/5.1 81 67.9/5.1 405 68.2/5.1 1013 5.0 12.5

3 6/19/2013 54 5054 M H 54 30.6˚ II 16614 69.0/6.0 65 69.1/6.0 65 69.5/6.0 325 68.9/6.0 813 5.0 12.5

4 6/19/2013 82 0760 F C 27 30.2˚ II 1695 67.9/6.3 52 67.3/6.3 52 67.1/6.3 208 67.4/6.3 650 4.0 12.5

5 6/20/2013 70 0070 F H 27 20.8˚ III 1695 67.1/6.1 81 66.7/6.1 81 66.3/6.1 324 67.4/6.1 1013 4.0 12.5

6 6/20/2013 72 4803 M H 26 25.3˚ III 11471 67.8/5.9 81 68.5/5.9 81 68.6/5.9 405 67.3/5.9 1013 5.0 12.5

7 6/20/2013 64 8133 F H 46 21.9˚ III 1695 67.9/6.4 101 66.8/6.4 101 67.5/6.4 404 67.3/6.4 1263 4.0 12.5

8 6/20/2013 78 2382 F C 23 33.2˚ II 16614 67.1/6.0 65 67.3/6.0 65 67.2/6.0 260 66.9/6.0 650 4.0 10.0

9 6/21/2013 70 3866 M H 42 29.6˚ II 1695 69.0/5.5 52 69.3/5.5 52 68.8/5.5 260 67.6/5.5 650 5.0 12.5

10 6/21/2013 76 9532 F C 24 35.3˚ II 4553 68.1/7.8 65 67.9/7.8 65 67.1/7.8 260 66.9/7.8 813 4.0 12.5

MEAN UVA-PF 4.4 12.0

STANDARD DEV (s) 0.5 1.1

STD. ERROR 0.2 0.3

S.E. % OF MEAN 3.6% 2.8%

t 2.262 2.262

95% CONFIDENCE INTERVAL 4.0 - 4.8 11.3 - 12.7

N OF CASES 10 10

MPPDD: Minimal Persistent Pigment Darkening Dose

I: Intensity of light source

The result of “10” is the result of dividing the protected skin value by the

unprotected skin value and the expected result is 1/3rd of SPF claim. Industrial

Sunscreen claims SPF 30 so the expected value is 10 and the result is 10 as seen above.

ISO 24444 In-Vivo Determination of the SPF (Sun Protection Factor) for International

Standards

Definition:

ISO 24444 is an In-Vivo test method (again requiring 10 human test subjects) determining the SPF,

or UVB protection, value of the sunscreen. This is primarily used in Europe but accepted

Internationally.

EVALUATION OF SUN PROTECTION BY SPF DETERMINATION (ISO 24444) – IN VIVO STATIC

Table

Sponsor: R&R Lotion AMA Lab No.: N-0479 Client No.: SUNSCREEN # 859 exp: 4-15 Expected SPF Value: 30

No. Date Subject Sex Race Age ITAo Skin Lamp MED/ MEDu I MED/ MEDu II MED/ MEDp MED/ MEDp SPF (P3) SPF Tech.

Type No. Hr &

Amps Hr &

Amps Hr &

Amps P3

Hr & Amps

Product Standard Value Initial

ID # I (Sec) II (Sec) P3 (Sec) (Sec)

1 6/18/2013 62 7072 F C 49 62.1° I 1695 126.6/5.7 4 126.9/5.7 4 126.1/5.7 75 126.5/5.7 151 18.8 37.8 2 6/18/2013 82 9684 F C 23 62.8° II 16613 127.1/6.0 4 127.4/6.0 4 127.6/6.0 60 128.1/6.0 151 15.0 37.8 3 6/19/2013 72 7921 F C 51 58.9° I 16614 128.1/6.0 4 128.4/6.0 4 128.9/6.0 75 128.4/6.0 151 18.8 37.8 4 6/19/2013 72 2368 F C 47 57.6° I 7528 128.4/5.7 4 128.6/5.7 4 128.3/5.7 60 128.0/5.7 134 15.0 33.5 5 6/19/2013 78 2670 F C 22 58.1° I 1695 128.2/6.7 4 128.8/6.7 4 128.0/6.7 75 126.6/6.7 134 18.8 33.5 6 6/19/2013 78 8873 M C 23 51.4° II 11471 126.1/7.9 4 125.7/7.9 4 125.5/7.9 75 125.3/7.9 151 18.8 37.8 7 6/20/2013 88 7551 F C 23 46.8° II 7528 127.2/5.8 8 127.4/5.8 8 127.0/5.8 120 127.6/5.8 269 15.0 33.6 8 6/20/2013 70 5092 M C 57 36.5° III 4553 126.7/6.3 11 127.2/6.3 11 127.0/6.3 165 126.8/6.3 414 15.0 37.6 9 6/20/2013 50 3379 F C 56 32.6° II 16614 126.1/6.0 6 126.3/6.0 6 126.6/6.0 113 127.2/6.0 202 18.8 33.7 10 6/20/2013 56 2212 F C 48 36.3° III 11471 128.6/7.3 6 128.8/7.3 6 129.4/7.3 113 129.1/7.3 202 18.8 33.7

MEAN (x) 17.3 35.7

STANDARD DEV (s) 2.0 2.2

STD. ERROR 0.6 0.7

S.E. % OF MEAN 3.5 2.0

95% CONFIDENCE INTERVAL 15.9 - 18.7 34.1 - 37.3

N 10 10

MED: Minimal Erythemal Dose I: Intensity of light source

Technicians: KC = Kaitlyn Callaghan, B.S. (Candidate)

ET = Erica Tavormina, B.S.

JR = Jaime Reidy, A.A.

TG = Tara Grube, B.S.

MED: Minimal Erythemal Dose (UV light exposure) I: Intensity of light source SPF Value: Value of Industrial Sunscreen’s SPF

ISO 24444 In-Vivo Determination of the SPF (Sun Protection

Factor) for International Standards

Definition:

ISO 24444 is an In-Vivo test method (again requiring 10 human test

subjects) determining the SPF, or UVB protection, value of the

sunscreen. This is primarily used in Europe but accepted Internationally.

COLIPA In-Vitro Method for Sunscreen Critical Wavelength

Determination for Broad Spectrum Claims

Definition:

This is a machine test that uses the COLIPA method to test the critical

wavelength on the sunscreen sample. The critical wavelength is

between 0 to 400nm and to claim broad spectrum a sunscreen must

cover up to 370 nm.

COLIPA Sun Protection By SPF Determination – 80 Minute Water Resistant

Definition:

COLIPA regulation and determination of the SPF (Sun Protection Factor) pre-exposure and post

exposure after immersion in water. This test is on humans using 10 participants of varying skin

pigmentation. Water resistance test claims can be 0 minutes, 40 minutes or 80 minutes. The

sunscreen has to meet the same SPF value as claimed on label before and after immersion.

I: Intensity of light source MED: Amount of UV exposure dosage (1 is low 12 is high) W: Wet (Post immersed in water)

ST: Static (Non immersed in water)

WRR: Water Resistant Retention

The last three results are the most important because they show that before being immersed in water Industrial Sunscreen has an SPF value of 35.7. After immersion the SPF factor is 32.1 and lastly that 90.1% of the UV light was blocked after immersion in water.

EVALUATION OF SUN PROTECTION

BY SPF DETERMINATION (COLIPA) - VERY WATER RESISTANT

Table

Sponsor: R&R Lotion

AMA Lab No.: N-0479

Client No.: SUNSCREEN # 859 exp: 4-15

Subject Sex Age MED/ I Skin MEDu I MEDu II MEDu II MEDp MEDp MEDp MEDp SPF (P3) VWR (P2) SPF Values %

ID #

Hr (Amps) Type

P3 P2 ST VWR (Sec) ST(Sec) W(Sec) (Sec) (Sec) (Sec) (Sec) Standard Control Static VWR VWRR

62 7072 F 49 126.9 5.7 I 4 4 4 75 60 151 134 18.8 15.0 37.8 33.5 88.3%

82 9684 F 23 127.4 6.0 II 4 4 4 60 60 151 120 15.0 15.0 37.8 30.0 78.8%

72 7921 F 51 128.4 6.0 I 4 4 4 75 48 151 134 18.8 12.0 37.8 33.5 88.3%

72 2368 F 47 128.6 5.7 I 4 4 4 60 60 134 120 15.0 15.0 33.5 30.0 89.2%

78 2670 F 22 128.8 6.7 I 4 4 4 75 60 134 134 18.8 15.0 33.5 33.5 100.0%

78 8873 M 23 125.7 7.9 II 4 4 4 75 48 151 120 18.8 12.0 37.8 30.0 78.8%

88 7551 F 23 127.4 5.8 II 8 8 8 120 96 269 269 15.0 12.0 33.6 33.6 100.0%

70 5092 M 57 127.2 6.3 III 11 11 11 165 132 414 370 15.0 12.0 37.6 33.6 89.1%

50 3379 F 56 126.3 6.0 II 6 6 6 113 90 202 202 18.8 15.0 33.7 33.7 100.0%

56 2212 F 48 128.8 7.3 III 6 6 6 113 90 202 180 18.8 15.0 33.7 30.0 88.7%

MEAN (x)

17.3 13.8 35.7 32.1 90.1%

STANDARD DEV (s) 2.0 1.5 2.2 1.8 7.9

STD. ERROR 0.6 0.5 0.7 0.6 S.E. % OF MEAN 3.5 3.6 2.0 1.9 95% CI

±1.6 ±1.3

95 % CI % OF MEAN

±4.4% ±4.0%

90 % Unilateral Confidence Limit (mean % VWRR -d)

86.6%

N 10 10 10 10 10