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Improving RAST annotations within RAST

Veronika VonsteinFellowship for Interpretation of Genomes

December 2008

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Your genome after RAST

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SEED genome in RAST

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Use Function based comparison tool

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Choose genome to compare to

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Compare Metabolic Reconstruction of your genome (A) and the SEED

genome (B)

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1. Case: Candidate present but Function different

But to eliminate the risk of annotating a paralog

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Compare Regions

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Annotate Chromosomal Clusters

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Annotate set 1

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Set 1 done continue

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2. Case: The real missing

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3. Case: Missed gene call

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Your genome after RAST

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Your improved genome

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Summary

• Re-annotated 490 gene functions• Increased the number of pegs in

subsystems from 36% to 50%• This added 60 subsystems to the

metabolic reconstruction of your genome• Possible to add missed genes, annotate

them and connect them to subsystems