Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 1
Food Traceability: the key to global market access
Session 5: The European Program – New science in traceability
IMPLEMENTING A NETWORKING PLATFORM
TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL CO-OPERATION WITH EUROPE
IN FOOD QUALITY AND SAFETY
Food Traceability: the key to global market access
1st and 2nd of April 2008, Kuala Lumpur, Malaysia
Mathieu WEIL, Caroline BLACHE, Fabrice DAVRIEUX,
Didier MONTET, Marc LEBRUN, Dominique PALLET
Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 2
Food Traceability: the key to global market access
IMPLEMENTING A NETWORKING PLATFORM
TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL CO-OPERATION WITH EUROPE
IN FOOD QUALITY AND SAFETY
CONTENTS
1. What is Cirad ?
2. Presentation of the ALCUE Food project
3. A few Cirad’s researches on traceability using innovative tools for Food Quality and Safety
- Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis
- Electronic nose
- Near Infra Red Spectrometry
Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 3
Food Traceability: the key to global market access
French Agricultural Research Centrefor International Development
workingworking for for developingdeveloping countries and the countries and the French French overseasoverseas regionsregions
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Food Traceability: the key to global market access
Six strategic lines of researchEcological intensification
Biomass energy in favour of communities in the South
Safe, varied food
Animal health and emerging diseases
Public policy, poverty and inequality
Relations between agriculture, environment,nature and society
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Food Traceability: the key to global market access
One operating principle: partnerships
Projects built and implemented hand-in-hand withresearch and development players• • ResearchResearch organizationsorganizations and and universitiesuniversities
• Professional • Professional organizationsorganizations and the and the privateprivate sectorsector
• Public • Public authoritiesauthorities and and donordonor agenciesagencies
• Local • Local authoritiesauthorities
• • NGOsNGOs
Working in the South
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Food Traceability: the key to global market access
3 Departments - 60 research units1800 persons including 750 researchers
Metropolitan France: 430 researchers
Outside metropolitan France: 320 researchersFrench overseas regions = 110Other countries = 210, including 30 in international research centres
Cirad’s Organization
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Food Traceability: the key to global market access
www.alcuefood.orgwww.alcuefood.org
“From European fork to Latin American farm”: an innovative networkingplatform for EU – LAC partnerships in food quality and safety R&D”
EC FP6 SSA Alcue-Food, 2005-08
2.
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Food Traceability: the key to global market access
MAIN GOAL
To strenghthen the EU-LAC R&D cooperation through the effective involvement of LAC partners in european consortium on FQS
GENERAL OBJECTIVE
To implement a permanent EU-LAC platform on FQS
To develop and implement a
comprehensiveEU-LAC
information system on FQS
issues
SPECIFIC OBJECTIVES
To define EU-LAC stakes on FQS and state
priorities on elaboration of
joint R&D projects
To enhance the LAC capacity
building throughbetter use of the existing EU-LAC
cooperationinstruments
To propose to EC and/or europeanconsortia relevant
LAC "groups of excellence" for
further involvment in the new FP 6&7
instruments
Project’s objectives
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Food Traceability: the key to global market access
KEY DATAS
Full project time : 36 months
Total human resources : 121 person months – 13 partners
4 EU Member States (France, Belgium, Spain, Portugal)4 Latin American MERCOSUR countries (Argentina, Brazil, Chile, Uruguay)
Total Budget : 737 500 euros
EXPECTED RESULTS
Strenghthening of trade in Food Sector
Compliance of LAC Food Exports with EU regulations
PROJECT CUSTOMERS
All stakeholders of the food supply chain : producers, firms, consumer associations, academic and research sector, policy makers, regulatory bodies
A totalfood chainapproach
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Food Traceability: the key to global market access
TraceabilityTraceabilitywaswas identifiedidentified as one of major as one of major prioritypriority topicstopics
(existence of a knowledge, existence of research activities …)
Organization of Workshopsand
Training sessions
Elaborationof
joint projects
Data baseimplementation
Information System(Web portal)
Disseminationactivities
Projectactivities
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Food Traceability: the key to global market access
Field: Traceability in the food chainField: Traceability in the food chain
Organization of workshops and training sessionsOrganization of workshops and training sessions
“Geo information in traceability systems, agricultural production systems and environmental management : opportunities in regional development”Campinas, Brazil, 27th of July 2005, 35 participants
“Recent advances on food traceability systems”Santiago, Chile, 1st of August 2005, 95 participants
“Opportunities for EU-LAC co-operation on food traceability systems”Santiago, Chile, 2-3 August 2005 (60 participants)
“Traceability systems along the meat chain”Buenos Aires, 4th of August 2005 (60 participants)
“Strategic Technology Watch - applied to traceability, why and how to use Internet efficiently”Buenos Aires, 28 November - 2 December 2005 (15 persons trained)
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Food Traceability: the key to global market access
Offer interface with INCO (southern cone) countriesto FP6 project (SSA PETER)
Facilitate integration of INCO partners in the EC top-upprocedure (joining Integrated Projects or Network of Excellence)
Elaboration of new projects for the FP6/FP7(ex: SSA OTAG -Geodecision to Track and Trace Agricultural Production)
Elaboration of joint EUElaboration of joint EU--LAC ProjectsLAC Projects
Field: Traceability in the food chainField: Traceability in the food chain
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Food Traceability: the key to global market access
Building a Database in traceability between the two regionsfor Key Stakeholders:
Producers, Entreprises and Traders
Scientists and Research Institutions
Consumer Groups and Policy Makers
Data base implementationData base implementation
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Food Traceability: the key to global market access
Information System (www.alcuefood.org)Information System (www.alcuefood.org)
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Food Traceability: the key to global market access
Through the Information System / Web Portal
Through Workshops and Trainings
Through Participation in PETER:- Disseminate European information to LAC/INCO countries- Put forward LAC/INCO demands/contexts for betteradequation of traceability systems to the real needs
Dissemination activitiesDissemination activities
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Food Traceability: the key to global market access
developped in Joint Research Unit QualiSud
- PCR DGGE (fishes)
- Electronic nose (dates)
- Near Infra Red Spectrometry (coffee and cocoa)
3. A few Cirad’s researches on traceability usinginnovative tools for Food Quality and Safety
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Food Traceability: the key to global market access
PangasiculturePangasiculture in Mekong delta (100.000 tons per year)in Mekong delta (100.000 tons per year)
Microbial ecology to trace originby Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis
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Food Traceability: the key to global market access
New method PCR-DGGE
Food sample
Total DNA includingmicrobial DNA and
Food DNA
Mixture of DNA amplicons from
species of microorganisms
Extraction of total DNA
PCR Amplification of a ribosomal DNA variable region by specific
primers
Comparative analysis of sequences and determination of microbial markers of specific area
Separation of differentDNA by DGGE
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Food Traceability: the key to global market access
Dendrogramme de 25 Obs.Moy. non pond. grpes associés
Percent disagreement
0 20 40 60 80 100 120
(Dlien/Dmax)*100
AGB2P5-PAGB2P4-PAGB2P3-PAGB2P2-PAGB2P1-PAGB5P5-PAGB5P4-PAGB5P3-PAGB5P2-PAGB5P1-PAGB4P5-PAGB4P4-PAGB4P3-PAGB4P2-PAGB4P1-PAGB3P5-PAGB3P4-PAGB3P3-PAGB3P2-PAGB3P1-PAGB1P5-PAGB1P4-PAGB1P3-PAGB1P2-PAGB1P1-P
DGGE
PCR-DGGE
DGGE
UV transilluminator
Statistical analysis
Image analysis
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Food Traceability: the key to global market access
DGGE prints of bacteria extracted from dry fishescoming from three different farms (rainy season)
Chau Phu An Phu Chau Doc
E.Coli
L. plantarum
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Food Traceability: the key to global market access
CP1-RCP2-RCP3-RCP4-RCP5-R
CP1-DCP2-DCP3-DCP4-DCP5-D
AP1-RAP2-RAP3-RAP4-RAP5-RAP1-DAP2-DAP3-DAP4-DAP5-D
CD1-RCD2-RCD3-RCD4-RCD5-R
CD1 -DCD2 -DCD3 -DCD4 -DCD5 -DTC1-RTC2-RTC3-RTC4-RTC5-R
TC1-DTC2-DTC3-DTC4-DTC5-D
-12 -10 -8 -6 -4 -2 0 2 4 6 8 10
Dimen s ion 1 : 46.24% varia nces
-8
-6
-4
-2
0
2
4
6
8
Dim
ensi
on 2
: 26
.40%
var
ianc
es
CP: Chau Phu; CD: Chau Doc; TC: Tan Chau; AP: An PhuR: rainy season; D: dry season
Factorial analysis of DGGE fingerprint of bacteria from fishes of 4 districts in the province of Angiang (Vietnam) at rainy and dry seasons
Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 22
Food Traceability: the key to global market access
Élec
tron
icH
uman
Electronic nose to distinguish dates [email protected]
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Food Traceability: the key to global market access
Electronic noseMass spectrometer+ sensors
Visualization of the global finger prints
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Food Traceability: the key to global market access
Distribution of Black Bousthammi, Deglet nour and their mix 50%on first principal plan
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Food Traceability: the key to global market access
Near Infra Red Spectrometry
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Food Traceability: the key to global market access
Near Infra Red Spectrometry: principle
Diatomic vibring Molecule
Infraredlight
(ν v, Ev)
(ν, λ) E = hν
The SPIR is the measurement of the absorption (Wave length and intensity) of the near infra-red light by a sample. The principle of the technic is based on the vibrational properties of the molecules and their interactions with the light.
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Food Traceability: the key to global market access
ON
C H3
N
NNH3C
OC H
3
Spectral fingerprintComparaison to spectral data base
Infrared Light
Energy absorbance by Chemical Links Cafein
Qualitative AnalysisQuantitative Analysis
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Food Traceability: the key to global market access
Characterization of coffee mixtures (roasted and grounded)
Robusta
Arabica
C P1 (63.86% )
C P2 (29.85% )
Distribution of coffee arabica and robusta on first principal plan
decaffeinated
Aromatized
with vanilla
defatted
Comparison of specific coffees to standard
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Food Traceability: the key to global market access
Linear discriminant analysis of three origins of cocoa beans
-1
-0.8
-0.6
-0.4
-0.2
0
0.2
0.4
0.6
0.8
1
1.2
-1 -0.5 0 0.5 1 1.5 2
-- axe 1 (55% ) -->
-- ax
e 2
(45%
) -->
Côte d'Ivoire
TrinidadVenezuela
Classification rate 100%
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Food Traceability: the key to global market access
ManyMany ThanksThanks for for youryour attention !attention !
www.cirad.fr/ur/qualite_aliments
www.cirad.fr
www.alcuefood.org
Mathieu WEIL [email protected]
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