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Transcript
Page 1: IEC CL10 Centrifuge User’s Manual · Packing List 11210900 Centrifuge IEC CL10 230V 50/60 Hz ... CL10 230V 50/60 Hz 11210901 Centrifuge IEC CL10 120V 60 Hz 11210902 Centrifuge IEC

IEC CL10 Centrifuge

User’s Manual

Analyze • Detect • Measure • ControlTM

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IEC CL10 Centrifuge

IEC CL10 Centrifuge

User’s Manual

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Carefully read this manual before operating your instrument.

Information contained in this document is the property of Thermo Electron Corporation; it may not beduplicated or distribuited without the owner’s authorization.

The validity of the guarantee is subject to the observation of the instructionsand precautions described in this document.

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Thermo Electron Corporation

MANUAL NUMBER

REV ECR/ECN DATE DESCRIPTION BY

a 11290085 03/06 Initial release

Preface

IEC CL10 i

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ii IEC CL10 Thermo Electron Corporation

Packing List

� 11210900 Centrifuge IEC CL10 230V 50/60 Hz� 11210901 Centrifuge IEC CL10 120V 60 Hz� 11210902 Centrifuge IEC CL10 100V 50/60 Hz

Item number Quantity Check

Centrifuge 1 �

User manual 1 �

Manual lid opening instructions 1 �

64227001 Mains cord 1 �

Toolbox:

11202979 Rotor removal tool 1 �

25535010 Emergency unlocking tool 1 �

26387032 Spare fuses 2 �

For missing parts call your nearest Thermo representative

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IEC CL10 iiiThermo Electron Corporation

WEEE Compliance

WEEE Compliance. This products is required to comply with theEuropean Union’s Waste Electrical & Electronic Equipment (WEEE)Directive 2002/96EC. It is marked with the following symbol. ThermoElectron has contracted with one or more recycling/disposal companiesin each EU Member State, and this products should be disposed of orrecycling through them. Further information on Thermo Electron’scompliance with these Directives, the recyclers in your country, andinformation on Thermo Electron products which may assist the detec-tion of substances subject to the RoHS Directive are available atwww.thermo.com/WEEERoHS

WEEE Konformittät. Dieses Produkt muss die EU Waste Electrical &Electronic Equipment (WEEE) Richtlinie 2002/96ECerfüllen. DasProdukt ist durch folgendes Symbol gekennzeichnet. Thermo Electronhat Vereinbarungen getroffen mit Verwertungs-/Entsorgungsanlagen inallen EU-Mitgliederstaaten und dieses Produkt muss durch dieseFirmen widerverwetet oder entsorgt werden. Mehr Informationen überdie Einhaltung dieser Anweisungen durch Thermo Electron, dieVerwerter und Hinweise die Ihnen nützlich sein können, die ThermoElectron Produkte zu identizfizieren, die unter diese RoHS Anweisungfallen, finden Sie unter www.thermo.com/WEEERoHS

Conformità WEEE. Questo prodotto deve rispondere alla direttivadell’Unione Europea 2002/96EC in merito ai Rifiuti degli ApparecchiElettrici ed Elettronici (WEEE). È marcato col seguente simbolo.Thermo Electron ha stipulato contratti con una o diverse società diriciclaggio/smaltimento in ognuno degli Stati Membri Europei. Questoprodotto verrà smaltito o riciclato tramite queste medesime. Ulterioriinformazioni sulla conformità di Thermo Electron con questeDirettive, l’elenco delle ditte di riciclaggio nel Vostro paese e informa-zioni sui prodotti Thermo che possono essere utili alla rilevazione disostanze soggette alla Direttiva RoHS sono disponibili sul sitowww.thermo.com/WEEERoHS

WEEE. Este producto requiere cumplir con la Directiva 2002/96ECWEEE (residuos de quipos electricos y electronicos). Este producto estamarcado con el simbolo WEEE, como se describe. En cada EstadoMiembro de la Union Europea (EU), Thermo Electron ha contratadocon diversas companias para disponer y/o re-ciclar los residuos de equi-pos electricos y electronicos. Existe information adictional acerca de laconformidad de Thermo Electron con esta Directiva, incluyendo losnombres de las diversas companias autorizadas para disponer/recyclarnuestros productos. Adicionalmente suministramos informacion acerca

Great Britain

Deutschland

Italia

Espana

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iv IEC CL10 Thermo Electron Corporation

WEEE Compliance

de los productos de Thermo Electron que puedes asistir en la deteccionde substancian mensionades en la directiva RoHS, explicado enwww.thermo.com/WEEERoHS

Conformité WEEE. Ce produit doit être conforme à la directive euro-péenne (2002/96EC) des Déchets d’Equipements Electriques etElectroniques (DEEE). Il est merqué par le symbole suivant. ThermoElectron s’est associé avec une ou plusieurs compagnies de recyclagedans chaque état membre de l’union européenne et ce produit devraitêtre collecté ou recyclé par celles-ci. Davantage d’informations sur laconformité de Thermo Electron à ces directives, les recycleurs dansvotre pays et les informations sur les produits Thermo Electron quipeuvent aider le détection des substances sujettes à la directive RoHSsont disponibles sur www.thermo.com/WEEERoHS

France

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IEC CL10 vThermo Electron Corporation

Declaration of compliance

We, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200CHATEAU-GONTIER, France, declare that the instrument definedhere below complies with the following European directives:

Nous, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200CHATEAU-GONTIER, France, déclarons que le matériel désigné ci-après est conforme aux directives européennes suivantes:

Wir, Thermo Electron Industries S.A.S. , Z.I. d'Aze Bellitourne 53200,CHATEAU-GONTIER, France, erklären dass das hier beschriebeneGerät sich in Übereinstimmung mit folgenden europäischenRichtlinien befindet:

Nostros, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne53200 CHATEAU-GONTIER, France, declaramos que el instrumentobajo descrito está fabricado según las especificaciones establecidas porlas siguientes normas europeas:

Nós, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200CHATEAU-GONTIER, France, declaramos que o instrumento aquiapresentado está de acordo com as normas européias abaixo listadas:

Noi, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200CHATEAU-GONTIER, France, dichiariamo che lo strumento sottodescritto è conforme alle seguenti Direttive europee:

• Machinery: 98 / 37 / CEE

• Low voltage: 72 / 23 / CEE - 93 / 68 CEE

• EMC: 89 / 336 CEE - 92 / 31 CEE - 93 / 68 CEE

• Safety standard: EN 61010-1 - EN 61010-2-020

Château-Gontier, 28th June 2005

Managing DirectorChristian Galiana

Declaration de conformite

Declaration of compliance

Konformitätserklärung

Declaración de conformidad

Declaraçao de conformidade

Dichiarazione di conformità

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vi IEC CL10 Thermo Electron Corporation

Guarantee Terms

Thermo guarantees that this unit is free from defects in materials andworkmanship when it leaves the factory, and will replace or repair theunit if it proves defective in normal use or during service for a period ofONE YEAR from the delivery.

This guarantee is invalid if the unit is incorrectly used, poorly servicedor neglected, mis-used or accidentally damaged.

There is no explicit guarantee other than as stated above.

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IEC CL10 viiThermo Electron Corporation

For further information,assistance or service

United States of AmericaTel.: +1 800 522 77 46Fax: +1 866 984 37 66

Canada or Latin AmericaTel.: +1 800 522 77 46Fax: +1 866 984 37 66

China (Shanghai)Tel.: +86 21 68 65 45 88Fax: +86 21 6445 7907, +86 21 6445 7830

India (Mumbai)Tel.: +91 22 5542 9494Fax: +91 22 5542 9495

JapanTel.: +81 454 53 9220Fax:+ 81 454 53 9222

Other Asia-Pacific CountriesTel.: +852 28 85 46 13

AustriaTel.: +43 (1) 80 14 00

FranceTel.: +33 1 69 18 77 77+33 2 28 03 20 00

BelgiumTel.: +32 24 82 30 30

GermanyTel.: +49 61 84 90 69 40Fax: +49 61 03 40 81 012

FinlandTel.: +35 89 32 91 00

ItalyTel.: +39 02 95 05 91

NetherlandsTel.: +31 7 65 71 44 40

Russia/CisTel.: +7 09 52 25 11 15

Spain and PortugalTel.: +34 9 32 23 31 54

SwitzerlandTel.: +41 (1) 4 54 12 12

United Kingdom and IrelandTel.: +44 87 06 09 92 03

Other Europe, Middle East, or AfricaTel.: +49 61 8490 6940Tel.: +33 2 2803 2000

In Other Countries:Contact your local THERMO products distributoror agent.

E-mail Technical Service Representative forproducts at:[email protected]

Visit our web site at: www.thermo.com

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Contents

Manufacturer ..............................................................................................1-1Advice to the user in safety terms ......................................................1-2Safety devices and notices..................................................................1-2User Manual......................................................................................1-2Warning markings ............................................................................1-2

Installation ..................................................................................................2-1Transport, Packaging, Lifting. ..........................................................2-1Installation ......................................................................................2-1Mains Wiring ....................................................................................2-1Environmental conditions ................................................................2-1Lid Unlock........................................................................................2-2Emergency lid unlock ......................................................................2-2Preliminary inspection ......................................................................2-3Assembly of Rotating Equipment ....................................................2-4Rotating head removing ..................................................................2-4

Characteristics............................................................................................3-1Dimensions and Weight ....................................................................3-1Centrifugation characteristics ..........................................................3-1Electrical characteristics ....................................................................3-1Specifications ..................................................................................3-1

Description and Performance..................................................................4-1Functional principles ........................................................................4-1Drive system......................................................................................4-1Safety interlock system ......................................................................4-1Imbalance Sensor ..............................................................................4-1Rotating equipment - Performance....................................................4-2Rotating equipment - Table ..............................................................4-2Performance ......................................................................................4-2RCF Calculation ..............................................................................4-3Front Panel........................................................................................4-4

Operation Procedure ................................................................................5-1Instructions to the operator ..............................................................5-1Power Switch - Cover unlock ............................................................5-1Loading balance ..............................................................................5-1SET parameters ................................................................................5-3Acceleration and Deceleration. Soft key.............................................5-3

Chapter 1

Chapter 2

Chapter 3

Chapter 4

Chapter 5

viii IEC CL10 Thermo Electron Corporation

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IEC CL10 ixThermo Electron Corporation

Contents

Setting and changing of rpm, min parameters ..................................5-4Starting the centrifuge ......................................................................5-3Stopping the centrifuge ....................................................................5-3Messages - Alarms ............................................................................5-4

Hazards, Precautions and Limitation of Use ........................................6-1Dangerous operations - working conditions to avoid ......................6-1EN 61010-2-020 Standard (IEC 1010-2-020) ..................................6-2Rotor and accessory precautions ......................................................6-2Corrosion information ....................................................................6-2Contamination hazards ....................................................................6-5

Routine Maintenance ................................................................................7-1Rotating Equipment and Centrifugation Chamber cleaning ............7-1Exterior cleaning ..............................................................................7-2Fuses ................................................................................................7-2

Chapter 6

Chapter 7

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Chapter 1 Manufacturer

• This centrifuge was properly manufactured and is safe and reliable.It could however become dangerous for the operator or for otherpeople if:

it is used by incompetent personnel or not according to theattached instructions ▲

it is used for different purposes from the ones it was built for ▲

it is not correctly earthed ▲

• Normal use of the centrifuge could require the manipulation ofbiological and hazardous materials. The users and personnel ofmaintenance must have specific requirements of professionalism foreach substance they use; they must be trained to operate withsafety, as identified in the World Health Organization “LaboratoryBiosafety Manual”.

• The centrifuge cannot be electrically and/or mechanicallyconnected to other instruments.

• It is forbidden the use within the centrifuge of flammable orexplosive materials, as well as materials which chemically interactvigorously.

• As this centrifuge does not conform to anti-deflagration standards,it should be used exclusively in explosion proof areas.

• Maximum density of the centrifugation samples should not exceed1200 kg/m3.

• The centrifuge should be used exclusively with rotating equipmentspecified by instructions, in their integrity and only with originalaccessories.

• Only original Thermo Electron spare parts can be used.

• It is strictly forbidden to take any changes to the centrifuge bycustomers or other personnel not authorized.

• Do not use the centrifuge when it is damaged or not safelyoperating or irregularly. Advice authorized personnel bymanufacturer as soon as for every anomaly.

Advice to the user insafety terms

IEC CL10 1-1Thermo Electron Corporation

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Chapter 1Manufacturer

1-2 IEC CL10 Thermo Electron Corporation

In order to ensure both mechanical and electrical operational safety, theIEC CL10 centrifuge is provided with the following safety controlsystems, as detailed in the attached Declaration of Agreement:

• The centrifuge will not start unless the lid is locked.

• The lid cannot be opened during centrifugation, even in the eventof power failure.

• Safety electronic circuits for self-diagnosis (with micro - processorcontrol); any possible breakdown of circuits and sensors is shownon display and neutralized at the same time, preventing unsafeoperating conditions of the machine.

• Load imbalance safety device.

• Double metal protection casing; reinforced centrifugation chamberin AISI 304 stainless steel.

• Lid antifall device.

• Main bipolar switch.

• Individual certified test, with measurements and testing of leakagecurrents, earth connection resistance and applied voltage.

• Identification plates; operating and maintenance instructions.

This manual is part of the centrifuge and it should be conserved forfuture notes and consultations. Keep always the manual close to theappliance and in a safe place, so that it is always available.Thermo Electron invites all users to read this manual.

The following symbols are necessary for the operator to preserve theprotection afforded by the equipment and to preserve dangers.

On the main switch allows centrifuge operating. ▲

On the main switch disconnects the centrifuge from the main supply. ▲

This symbol means that you will find important information for safetyand if they are unobserved it could be taken some damages to applianceand/or operator. ▲

Only authorized personnel can operate with the parts where there is thesymbol and after switching off the mains supply. To operate with highvoltage could cause death. ▲

Safety devices andnotices

User Manual

Warning markings

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IEC CL10 2-1Thermo Electron Corporation

Transport, Packaging,Lifting

Installation

Mains Wiring

Chapter 2 Insatllation

During transport centrifuge is seated in a special packaging.All lifting and transporting must be carried out by using properhandling equipment and by people having undergone the necessarytraining.

1. General conditions accepted for centrifuge transport and storage are:

• Environment temperature -20°C + 50°C.

• Relative humidity up to 90% excluding moisture formation.

2. Unpack the centrifuge, carefully removing the rotating equipment,any possible accessories and the material supplied for ordinarymaintenance. Check the contents of packaging against the packinglist.

(Keep the packaging until the centrifuge has been tested).

The machine should be installed in a dust and corrosion free room.Place the centrifuge on a bench top, which must be rigid andsufficiently strong.

Leave a 300 mm space free on each side of the machine and behind forright ventilation. ▲

Check mains and frequency: they must correspond to values shown oninstrument identification label.IEC CL10 230 V ±10% 50/60 Hz (1 phase + ground)IEC CL10 120 V ±10% 60 Hz (1 phase + ground)IEC CL10 100 V ±10% 50/60 Hz (1 phase + ground)

Check that mains wiring are effectively grounded. ▲

Manufacturer declines all responsibility for any damages due to non-grounding of the machine.

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2-2 IEC CL10 Thermo Electron Corporation

Chapter 2Installation

• Indoor use.

• Temperature: 5°C to + 40°C.

• Maximum relative humidity of 80% for temperatures up to 31°Cdecreasing linearly to 50% relative humidity at 40°C.

• Installation category: II

• Pollution degree: 2

1. Switch the centrifuge on trough the main on the right side of theunit.

2. When the centrifuge is switched on but stationary: LID led on atthe opening.

3. The mechanic unlocks and consequent opening of the lid isobtained by pulling the lever on the right side of the machinetowards the centrifuge front.

Warning Before proceeding to installation operations, it is advisable todisconnect the centrifuge from the mains, by moving the POWERswitch on “O” position. ▲

4. After opening the lid, remove any possible packaging around thedriving spindle. Unscrew the equipment-blocking nut from theengine shaft.

5. Carefully clean the inside of the centrifugation chamber removingany packing residues. Due to the air - whirling, solid particlesaccidentally left in the centrifugation chamber would create anexcessive wear of the chamber itself and of the outer Equipmentrotating bottoms.

In the event of mains non-availability or power failure, the lid can beopened by using the special tool supplied with the centrifuge.

This operation should always be effected with the centrifuge mainsplug disconnected.

1. Unscrew the nylon screw in the hole next to opening lever, andhere below identified by the corresponding label.

2. Insert horizontally the unlocking tool supplied with the centrifugein the hole (fig. a).

Environmentalconditions

Lid Unlock

Emergency lid unlock

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3. Lift the unlocking tool upwards and at the same time pull the lever(fig. b).

4. At the end screw again the nylon screw.

Warning Should the emergency occur due to electricity failure, duringcentrifugation (E4 message will be shown when the power is on again),wait at least 5 minutes for the end of the Rotating Equipment rotation.Upon opening, lift the lid by hand and observe the rotor (be careful inthis operation): if it is still rotating, close the lid and wait. ▲

Before any centrifugation run, the rotating equipment should bethoroughly inspected for corrosion and cleanliness (see 6.3). The clip inthe center of the rotor and the drive spindle should also be clean andundamaged.Any failure to follow the above advice may have serious consequencesfor the safety of the appliance.If visible signals of corrosion or anomalies on the rotor or buckets arepresent, contact the authorized personnel. Also the centrifuge shouldbe observed: screw, lid hings, latch and corrosion. In case of problemscontact the technicians. ▲

Figure 1.

Figure 2.

Preliminaryinspection

IEC CL10 2-3Thermo Electron Corporation

Chapter 2Installation

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The rotor’s drive is made by means of one pin inserted in the rotor’sshaft, which must coincide with the milling of the rotor.

For a right mounting, follow instructions hereafter indicated:

• ensure that both the spindle of the motor and the hole of the headare clean;

• introduce the head in the centrifuge protective bowl and slip it intothe spindle with the milling of the collar face up;

• align the milling in the collar with the pin on the spindle;

• screw and strongly tighten (with the supplied key) the hexagonalnut on the threaded spindle of the motor unit (if previousoperations have not performed correctly, this phase will beimpossible as scheduled from the requirements of Safety StandardEN 61010).

Warning Be sure the rotating head has been correctly locked and therotating equipment assembled, before starting the centrifuge. ▲

For rotating equipment with accessible centers, unscrew with thesupplied wrench and lift with the fingers while pulling with the otherhand supporting the rotor.

Assembly of RotatingEquipment

Rotating headremoving

2-4 IEC CL10 Thermo Electron Corporation

Chapter 2Installation

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IEC CL10 3-1Thermo Electron Corporation

Chapter 3 Characteristics

Height x Width x Depth:................................28 x 37 x 46 cmNet Weight: ....................................................28 Kg

Max. allowable capacity:..................................with Swing-out rotor(4 x 135 ml) 540 x 10-6m3

........................................................................with Fixed-angle rotor (6 x 50 ml) 300 x 10-6m3

Max. allowable density: ..................................1200 Kg/m3

Max. allowable weight:....................................0.648 KgMax. speed: ....................................................6500 rpm

Max. RCF at Tip:............................................3684 xgWork temperature: ..........................................Ta + ∆TMax. noise:......................................................57 dBA

Nominal operating voltage: ..........230V ± 10% 50/60 Hz120V ± 10% 60 Hz100V ± 10% 50/60 Hz

Max peak current: ........................1,5 A (for 230V)2,5 A (for 120V)3,8 A (for 100V)

Max power (steady state):..............150 W

• Microprocessor controlled.

• Set/reading speed 300 – 6500 rpm (by step of 10 rpm) for eachpressing of the key; from 300 rpm step of 100 rpm if you press thekey continually more than 2 sec.); accuracy: ± 20 rpm.

• High or low acceleration.

• High or low deceleration.

• Set timer 1 min. up to 99 min. + continuous function.

• Electronic-accelerometric imbalance encoder.

• Direct driving system.

• Brushless induction drive motor.

Dimensions andWeight

Centrifugationcharacteristics

Electricalcharacteristics

Specifications

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4-1 IEC CL10 Thermo Electron Corporation

Chapter 4 Description and Performance

The IEC CL10 centrifuge was designed for laboratory use. Throughthe application of the Relative Centrifugal Force (RCF), it allows theseparation of substances made of different density elements. Swing outrotating equipment can generally carry a higher load than a fixed angleor aerodynamical equipment. The larger the diameter of a rotor, thegreater the load and the lower the maximum rpm Relative CentrifugalForce (RCF) generated on a centrifuged sample is directly proportionalto the sedimentation useful radius and to the speed value square.

A three phase asynchronous motor drives the rotating equipment. It issituated in the center of an armours plated centrifugation chamber.

The centrifuge is equipped with a double interlock system thatprevents opening of the centrifuge lid when the rotor is spinning. Thecentrifuge will not operate until the lid is closed and latched in place.The lid remains latched until rotor stops spinning.

Note If a power failure occurs, access to the samples in the centrifuge ispossible.For this it is necessary to use a special tool. Follow the lid openingprocedure emergency. ▲

Perform bypass under emergency conditions as the rotor could still berotating (see pag. 2-2).

The centrifuge is equipped with an accelerometric load imbalancedetector.

In case of excessive imbalance the machine stops whilst: this conditionis displayed with the message “INBL” which will disappear once the lidhas been opened to remove the imbalance cause (see 5.9).

The tolerated imbalance depends on the rotor in use. Carefully balancesample load to avoid actuating the imbalance detection system.

Functional principles

Drive system

Safety interlocksystem

Imbalance Sensor

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IEC CL10 4-2Thermo Electron Corporation

Chapter 4Description and Performance

Rotating equipment - Performance

Rotating equipment - Table

Rotating Equipment Rotor composition

Rotating Head Trunnion (set of 4) Bucket Sealing Lid

Cat.No. Capacity a Cat. n° n. Cat. n° n. Cat. n° Cat. n°

O-G26/1 11210014 4x135 ml 0-90° 11210021 - - 1 11210331 (set/4) 11210664*

O-G26/2 11210015 16x15 ml 0-90° 11210021 1 11210344 2 11210883 (set/8 -

O-G26/3 11210016 8x15 ml 0-90° 11210021 1 11210345 8 11203722 11203724

F-G1 11210018 6x50 ml con. 40° 11210022 - - 1 11210635 -

F-G3 11210019 24x15 ml 45° 11210023 - - 3 11210883 (set/8) -

A-G20 11210891 20x2.0/1.5 ml 45° - - - - - 11172479**

*on request

**not sealed

Performance

Rotating Equipment Max speed Max radius Max RCF Max ∆T Max Noise

[rpm] [m] [xg] [°C] [dBA]

O-G26/1 4000 0,148 2647 6,5 56

O-G26/2 4000 0,144 2575 10 57

O-G26/3 4000 0,144 2575 9 56

F-G1 5000 0,131 3661 10 57

F-G3 4000 0,133 2379 10 55

A-G20 6500 0,078 3684 5,5 50

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4-3 IEC CL10 Thermo Electron Corporation

Chapter 4Description and Performance

RCF Calculation

The main factors of the centrifuge action in terms of correct analysismethodology are: the centrifugation time and thegravity number “xg” (relative centrifuge acceleration) to which theproduct is submitted. The graph allows the determination, as afunction of the radius “r”, of the relative centrifuge acceleration value,expressed in multiples of the gravity acceleration value g (9.8 m/sec.)for each RPM value between 150 and 20,000.

Example To find the relative centrifuge acceleration value xg of a pointat a 100 mm (0.1m) distance from the rotation axis and rotating at3000 RPM, place a ruler on the radius scale on the left, joining thepoint marked 100 on the left scale with the one marked 3000 on theright hand scale of the RPM: the intersection of the central scale xggives directly the required relative centrifuge acceleration value (in ourcase 1000 xg). ▲

The following relations connect the relative centrifuge acceleration andthe RPM number:

xg = 1,1178 • 10-3 • n2 • r where n = RPM

n = r = radius in metersxg

1,1178 * 10-3* r

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IEC CL10 4-4Thermo Electron Corporation

Chapter 4Description and Performance

Front Panel

1. Speed display (rpm) shows, when the machine is stopped, SETrpm (set rev. per minute); when it is spinning, real rpm. With lightflashing shows SET rpm (from 300 to 6300 rpm).

2. Rpm keys for increasing or decreasing set rpm.

3. Minutes display shows, when the machine is stopped, setcentrifugation time; when it is running, remaining centrifugationtime; during “HOLD” function (continuos operation), showsseconds and minutes flying (from 1 to 59 sec., then from 1 to 99min; 0 min = Hold - Hd on display).

4. Minutes keys: for increasing or decreasing set centrifugation time.

5. STOP key for a manual stop of the centrifuge.

6. START key for starting of centrifugation cycle.

7. SOFT key to activate or de-activate, by sequence, low accelerationand low deceleration. When led is off (high acceleration anddeceleration), following functions will be activated by sequence:

• Slow deceleration

• Slow acceleration

• Slow acceleration and deceleration

• High acceleration and deceleration

Corresponding led will indicate activated soft function status.

8. Running led: flashes during acceleration and breaking down phase,fixed when real speed = SET rpm.

9. Lid opening led: it is on when the centrifuge is stationary.

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5-1 IEC CL10 Thermo Electron Corporation

Chapter 5 Opreation Procdure

1. The presence of the operator is necessary from the start untilcentrifuge reaches set speed.

2. It is necessary his presence also at the end of the centrifugation run.

1. By positioning the mains switch located on the right side of themachine on I, the centrifuge is connected to the mains.

Front panel will be in the same “Operative Mode” as in the lastworking cycle.

2. When the machine is stationary and with lid opening led on, it’spossible to unlock the lid. The mechanical unlock, with subsequentlid opening, is obtained by pulling towards the front of the unit thehandle on the centrifuge right side.

All rotating equipment are dynamically balanced at source; referencenumbers and marks indicate the exact respective positions of thevarious parts forming the equipment; when rotating, this should alwaysbe whole and complete (see fig. 1).

All buckets must be always inserted into the rotating equipment, evenfor partial loads.

In the load balance operation, buckets should never be weighed, butonly their contents (adapters, tubes, etc.).

Distribute the load correctly. Loads should be inserted two by two, ofequal weight, at 180 degrees from the rotation axis and at equaldistance (radius) from the center of rotation (see fig. 2).

Instructions to theoperator

Power Switch Cover unlock

Loading balance

Figure 1.

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For partial loads of swing-out rotating equipment (type O-) it isadvisable to distribute the tubes symmetrically also with regard to thecenter of rotation (see fig. 3).

Correct Incorrest

Avoid overloading rotating equipment, having to centrifuge liquidswith higher density than 1200 kg/m3, the max. rpm should be reducedaccording with the following formula:

Allowed rpm = Max. rpm

After having placed and locked the rotating equipment, lower the lid,then close it pressing on the front edges until the hook in the lid clicksinto the lid lock mechanism. Only this operation will activate thecentrifuge-starting device.

IEC CL10 5-2Thermo Electron Corporation

Chapter 5Operation Procedure

Figure 2.

Figure 3.

1200

New density

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5-3 IEC CL10 Thermo Electron Corporation

Chapter 5Operation Procedure

Starting thecentrifuge

Stopping thecentrifuge

Basic condition: Lid closed1. Press “START” key.

Centrifuge will accelerate following requested acceleration profile to theset speed,when running time is completely elapsed, deceleration willtake place.

• Led for lid opening goes off.

• Led for running will start flashing until set speed is reached andkept (fixed led lit).

• During run time, rpm display show real parameters, min displayelapsed run time in minutes “counts-up ” (if SET min = Hd =continuous mode) or remaining run time “counts-down ”

Note If set rpm is higher than Max speed of rotating Equipment (seePerformance Table), running led will continue flashing, indicating setanomaly. Change right SET rpm. ▲

During running time, to read and change set values, press one of twoSET cursors of each parameters:

• display starts flashing and shows set value for 1 second.

• during flashing, it is possible to change set value.

• during the run,it is possible to change requested deceleration (high or “SOFT”).

Once the set time has elapsed, the centrifuge automatically stops withthe set braking mode profile.

In any situation, press STOP key to anticipate stopping.

Note The centrifuge stop can also occurs automatically for:

• load imbalance

• sudden power failure

• error or breakdown ▲

In all cases the relative information will appear on the rpm display.

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IEC CL10 5-4Thermo Electron Corporation

Chapter 5Operation Procedure

Messages - Alarms The microprocessor visualizes on rpm display messages to inform orindicate errors, malfunctions or breakdowns, expressed in letters orcodes.

LINE It appears when mains value is close to the limits of the normal operating

range (see par. 2.3)

END At the end of every centrifugation cycle, stationary centrifuge.

This message disappears and will appear set rpm at the next operation (e.g.

pressing any key or opening the lid).

LID When START key is pressed with open or apparently closed lid (lid down but

not locked), on rpm display it will appear Lid + acoustic alarm. Lid must be

closed: the message disappears enabling to start the centrifuge by pushing

any key.

INBL When the load is not balanced, this message + acoustic alarm will appear.

Centrifuge will stop and it will be necessary to rebalance the load. The

message disappears when the lid is opened.

If this alarm appears immediately after pressing the START key, please

contact your authorized Technical Service

ACOUSTIC when set speed is higher that max speed allowed for that Rotating

PULSING Equipment.

ALLARM It will disappear by pressing STOP key or setting the right speed.

E 4 message together with an acoustic alarm means power failure, even short.

No key can be used and machine will stop by inertia. When it is stopped,

acoustic alarm will finish but message E 4 will be displayed again.

If power failure will go on (lid is automatically closed) and it is necessary to

open it, please refer duly to instructions mentioned in 2.6 “Emergency unlock”.

E 1 It appears when the START key is pressed without a rotating equipment

installed.

This operation must be avoided. If persists, please contact authorized

Technical Service.

E .. When E.... followed by a number appears, together with an acoustic alarm,

you have to contact authorized Technical Service.

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6-1 IEC CL10 Thermo Electron Corporation

Chapter 6 Hazards, Precautions andLimitation of Use

• Using the centrifuge if it has not been properly installed as perinstructions enclosed in this manual.

• Incorrect tightening of rotating head on driving stud and bucketassembly on the rotor.

• Lean on the machine when running.

• Stay in the controlled area (300 mm) more than required time.

• Depositing any object that could be dangerous in the free area.

• Moving or shifting the machine during centrifugation.

• Using the centrifuge with rotating equipment showing evidentcorrosion and wear marks.

• Using the centrifuge with rotating equipment and accessories notapproved by the manufacturer.

• Using the centrifuge in explosion risk rooms.

• Not balanced load, which causes an excessive vibration of thecentrifuge.

IF ANY DANGEROUS EVENT OCCURS, KEEP YOURSELFFAR AWAY FROM THE INSTRUMENT AND SWITCH

OFF THE MAIN POWER SOURCE

• Don’t insert all buckets into the rotating equipment, even forpartial loads.

• Leave the appliance exposed to the elements (rain, sun, etc.).

• Exceeding the maximum speed indicated in the “max. rpm”column of the performance table (see 4.2.2).

• Using old accessories on a new machine.

• Using tubes, bottles not suited for centrifugation.

Dangerous operationsworking conditions

to avoid

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IEC CL10 6-2Thermo Electron Corporation

Chapter 6Hazards, Precautions and Limitation of Usa

• Centrifuging samples with higher density than allowed to themaximum speed, without reducing the speed limit (see 5.2.6).

• Manipulating and/or tampering with the electronic and mechanicalparts.

En 61010-2-020 standard relative to laboratory centrifugesrecommends the user:

• To mark out a clearance envelope 300 mm around the centrifugeor establish special procedures for the non entry of all persons or alldangerous materials into this space during the operation of thecentrifuge.

• To provide an emergency stop switch enabling the mains power tobe cut in case of malfunction. The switch should be placed at a safedistance from the centrifuge, preferably in a room different fromthe one where the centrifuge is situated.

Thermo Electron rotors made of aluminium alloy are designed tooperate at their rated RCF for many years.With careful use they will resist corrosion, lowing the possibility ofexcessive imbalance, disruption and subsequent damage to theinstrument. The primary conditions for the initiation of corrosion existin every laboratory during daily use of the centrifuge. For this reason itis essential that due care and attention be paid to inspection andcleaning.

Chemical Corrosion

This corrosion is characterized by chemical reactions due to theexistence of any electrolyte liquid on the surface of the rotatingequipment.If these substances are allowed to remain on the surface the corrosionwill almost certainly occurs. This produces first a discoloration of theanodization the pitting of the metal.Acidic and alkaline solutions sustaining their pH level will createproblems of corrosion in aluminium equipment.Chlorides, present in salts or even in skin contact with the rotatingequipment are among the most aggressive and harmful substancescommonly found in the laboratory.The chemical products, which are the origin of this corrosion do notnecessarily originate from broken tubes, for example they could comefrom:

• chemical vapors present in the laboratory which are dissolved in theresidual humidity, in condensed water (refrigerated centrifuges)

EN 61010-2-020Standard

(IEC 1010-2-020)

Rotor and accessoryprecautions

Corrosion information

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6-3 IEC CL10 Thermo Electron Corporation

Chapter 6Hazards, Precautions and Limitation of Usa

present at the base of the rotor pockets.

• corrosive liquids originating from overfilled uncapped tubes (theliquid overflowing during centrifugation)

• Inserts, adapters, racks, bottles whose exterior has been soiled by achemical product or poorly rinsed after decontamination (withbleach, for example).

Note If the products are very corrosive, simply rinsing is insufficient.Residual traces dissolve little by little with the humidity present in thebottom of the rotor pocket. ▲

Beware of the presence of solid particles beneath tubes, inserts, racksor adapters. These particles are crushed by the centrifugal force andpenetrate the protective, anodized layer of buckets and rotors, thuscreating easy pathways for corrosion. ▲

Stress Corrosion

This term relates to the phenomenon of accelerated corrosion due tothe effect of centrifugation when a corrosive chemical is in contact withthe alloy. From the time when the aluminium alloy has been attackedby chemicals, stress corrosion begins to appear. As it is on a microscopicscale it is even more dangerous than a macroscopic since it is invisible.

During centrifugation, chemicals responsible for corrosion are alsosubmitted to the very high “g” force, which pushes them against thealloy. This close contact facilitates the chemical reaction which occursmuch faster than in a static situation. Moreover, centrifugal force isvery directional thus corrosion under stress creates, with a very smallamount of corrosive product, straight microscopic fissures. Eachcentrifugation run makes the chemical migrate further and further.Fissures or cracks, although it is microscopic, are a cut in the metal,breaking the cohesion of the material. As one weak link in a chainallows the chain to break, so the micro-fissures break the chain ofresistance of the accessory to centrifugal force. As accessories aredesigned with high levels of safety, rupture does not occur as soon asthe first micro-fissures are produced.Depending upon the location of the fissure, disruption may occurbefore it reaches the external surface of the accessory. The fissure createsa weakness, which makes the accessory less and less resistant tomechanical fatigue.The corrosion by a small amount of corrosive product does not disruptthe accessory but makes it mechanically weaker and weaker untildisruption due to both centrifugal effort and number of cycles.

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IEC CL10 6-4Thermo Electron Corporation

Chapter 6Hazards, Precautions and Limitation of Usa

Warning Because stress corrosion is largely invisible, it is essential thatrotating equipment are scrutinized regularly paying particular attentionto susceptible parts such as the bottom of pockets, the outer edges andthe base of the rotating equipment. ▲

Thermo Electron centrifuges are likely to be used in medical researchand laboratory where hazardous substances are frequently present.

Warning The user has the responsibility for carrying out appropriatedecontamination and elimination of the handled materials.Users should be aware on the internationally recognized “LaboratoryBiosafety Manual” , which gives information on decontaminants; theiruse; dilutions; properties; and potential applications. ▲

If decontamination procedures require the use of warming (i.e.autoclave) the rotating equipment should always be completelydisassembled before being subjected to heat and after external chemicalcleaning.Seals, Tubes and plastic components should be decontaminated withthe method most suitable for them, which might not be the same as forrotating equipment.

Note that the black color on the surface of the rotating equipment willbe gradually washed out if the rotor is regularly autoclaved or bleached.This does not necessarily denote degradation on the anodization. ▲

Warning ANY PART, WHICH HAS BEEN SUBJECTED TOTEMPERATURES ABOVE 130°C, MUST BE DISCARDED. ▲

Warning For any other unscheduled cleaning, the customer mustcontact the manufacturer to verify that the proposed method does notdamage the appliance. ▲

Thermo Electron makes no claims as to the effectiveness of proprietarybrands of decontaminating solutions.

Contamination hazards

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7-1 IEC CL10 Thermo Electron Corporation

Chapter 7 Routine Maintenance

Caution During maintenance operations, always disconnect thecentrifuge from the mains (fig. 7.1). ▲

Regular cleaning of rotating heads and centrifugation chamber is vital forcorrect work condition of the centrifuge (fig. 7.2).

The operator must wear suitable accessories during the cleaning (mask,gloves, special wears ...).Verify the integrity of biosafety components when they are used. Thesubstances used determinate also the correct methods for inspection ofthese components.However the user must verify always their good conditions also byinspection. Do not clean with materials could damage silicone andpolycarbonate. The regular maintenance for biosafety is important fordaily life efficiency.Ideally, rotating equipment should be washed after every run but at leastweekly in warm water containing a few drops of mild not alkalinedetergent (domestic washing up liquid is ideal) and EVERY TIMEAFTER A SPILLAGE HAS OCCURRED. Do not forget to wash thecore of the rotating head, which comes into contact with the drivespindle. Each pocket of the rotating heads aerodynamics and bucket ofswing-out equipment must be washed thoroughly using a small nylonbrush. Once the rotating head has been removed, it is easy to clean thecentrifugation chamber and gaskets, warm water with not alkalinedetergent is recommended. Do not use corrosive solvents.Once the Rotating Equipment is clean, rinse it by running water,preferably distilled.Dry the Rotating Equipment with a soft absorbent non-woven cloth ortissue. Drying may be finished off with warm air jet (e.g. a hair dryer).

DO NOT USE METAL WIRE BRUSHESAND MAKE CERTAIN THAT ROTATING HEADS

SLOTS AND BUCKETS ARE WELL-DRIED.

NEVER LEAVE damp Rotating Equipment on a metal surface,particularly stainless steel because an electrochemical reaction could takeplace with the aluminium or magnesium in the rotor. For swing-outrotating equipment, be sure to clean the dirty grease from the studs andreplace it with a small amount of fresh grease. This will ensure thebuckets to swing easily. The vast majority of apparent imbalanceproblems arise from the failure of the user to clean and grease the studsand not from instrument error.

Rotating Equipmentand CentrifugationChamber cleaning

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IEC CL10 7-2Thermo Electron Corporation

Chapter 7Routine Maintenance

Never apply grease if studs and balancing slots have not been carefullycleaned (fig. 7.3 a/b). Use only the same kind of grease furnished in theRotating Equipment packaging.

For a correct cleaning a light domestic use detergent is recommended.A mixture of 50% isopropilic alcohol and water is warmly suggestedbecause it will not damage the labels.

There are two fuses in the main tap.You can change them drawing off from the main tap (see fig. 7.4)

Fuses dia 5 x 20 mm 2AT / 250V IEC CL10 (230V 50-60Hz)dia 5 x 20 mm 4AT / 120V IEC CL10 (120V 60Hz)

Warning Before changing the fuses, disconnect the centrifuge from themains. ▲

In case of fuse blowing, do not replace them until the machine hasbeen inspected by a maintenance engineer.

Exterior cleaning

Fuses

Figure 7.4

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I-1 IEC CL10 Thermo Electron Corporation

Certificate of Decontamination and Cleaning

Certificate of Decontamination and Cleaning

Read carefully the instructions below before sending an instrument, orparts of it, to Thermo Service Dept. or to any Authorized TechnicalAssistance Service. ▲

Mr / Mrs (name) .................................................................... Establishment ............................

Department ......................................................................................................................................

Address ................................................................................... Post / Zip code ............................

City ......................................................................................... State / Country ..........................

Declares the cleaning and decontamination of the following:

Product type .................................................................................................... Serial N° ............

Rotor ................................................................................................................ Serial N° ............

Rotor ................................................................................................................ Serial N° ............

Accessory - Description ................................................................................... Serial N° ............

Accessory - Description ................................................................................... Serial N° ............

Nature of contamination

..........................................................................................................................................................

..........................................................................................................................................................

Decontamination Procedure used

..........................................................................................................................................................

..........................................................................................................................................................

Decontamination certified by:

Mr / Mrs ................................................................................ Institution ..................................

Date ........................................................................................ Signature ....................................

When an instrument, or parts of it, requires servicing by Thermopersonnel, the following procedure must be accomplished to ensurepersonnel safety:

• Clean the instrument and proceed to its decontamination from anykind of dangerous products.

• Compile this Decontamination Certificate with all the informationrequired.

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IEC CL10 I-2Thermo Electron Corporation

Certificate of Decontamination and Cleaning

• Attach this Certificate to the instrument (or part) before sending itto Thermo Service or other authorized technical service.

Thermo personnel will not accept to work on instruments deprived ofthis Decontamination Certificate. If an instrument is received at our Service facilities and, in our opinion,is a radioactive or biological hazard, the item will be refused and resentto the Customer. Disposition costs will be borne by the sender. Instructions for decontamination and cleaning are explained in theUser Manual. Additional certificates are available from your localtechnical or Customer Service representative. In the event thesecertificates are not available, a written statement certifying that theinstrument or part has been properly decontaminated and outlining theprocedures used will be acceptable.

For your convenience, use a copy of this page

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P/N 11290085-a