Re

ste

k C

orp

ora

tio

n

11

0 B

en

ne

r C

ircl

e

Be

lle

fon

te, P

A 1

68

23

1-8

14

-35

3-1

30

0

• 1

-80

0-3

56

-16

88

•

Fa

x: 1

-81

4-3

53

-13

09

•

ww

w.r

est

ek

.co

m

LC_

CF0

59

1

Fe

nta

ny

l, M

eta

bo

lite

, an

d A

na

log

ue

s in

Uri

ne

on

Ra

pto

r™ B

iph

en

yl

by

LC

-MS

/MS

Col

umn

Rapt

or ™

Bip

heny

l (ca

t.# 9

3095

52)

Dim

ensi

ons:

50

mm

x 2

.1 m

m ID

Part

icle

Siz

e:

5 µm

Tem

p.:

30 °C

Sam

ple

Conc

.: A

10

ng/m

L st

anda

rd w

as p

repa

red

in u

rine.

The

inte

rnal

sta

ndar

d w

as fo

rtifi

ed to

a fi

nal

co

ncen

trat

ion

of 1

ng/

mL.

An

80 µ

L al

iquo

t of t

he 1

0 ng

/mL

urin

e st

anda

rd w

as d

ilute

d w

ith

32

0 µL

of 7

0:30

wat

er:m

etha

nol s

olut

ion

(5-f

old

dilu

tion)

prio

r to

anal

ysis

.In

j. Vo

l.:

5 µL

Mob

ile P

hase

A

: 0.

1% F

orm

ic a

cid

in w

ater

B:

0.1%

For

mic

aci

d in

met

hano

l

Tim

e (m

in)

Flow

(mL/

min

) %

A

%B

0.

00

0.4

70

30

2.00

0.

4 20

80

2.

01

0.4

70

30

3.50

0.

4 70

30

Det

ecto

r M

S/M

SIo

n M

ode:

ES

I+In

stru

men

t H

PLC

Con

c.

Peak

s tR

(min

) (n

g/m

L)

Q1

Q3

Qua

ntifi

er

Q3

Qua

lifier

1.

N

orfe

ntan

yl

1.04

10

23

3.10

84

.08

150.

11

2.

Nor

fent

anyl

-d5

(IS)

1.02

1

238.

10

84.0

8 —

3.

A

cety

l fen

tany

l 1.

48

10

323.

29

188.

18

105.

10

4.

Ace

tyl f

enta

nyl-

13C6

1.

48

1 32

9.22

18

8.22

—

5.

Fe

ntan

yl

1.65

10

33

7.29

18

8.22

10

5.09

6.

Fe

ntan

yl-d

5 (IS

) 1.

64

1 34

2.35

18

8.22

—

7.

Su

fent

anil

1.84

10

38

7.35

23

8.18

11

1.07

8.

Su

fent

anil-

d5 (I

S)

1.83

1

392.

29

238.

18

—

0.0

1,2

3,4

5,6

7,8

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

Tim

e (m

in)

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