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Fast Protein Liquid
Chromatography (FPLC)
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Column components
1. Packing
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Gel degassing.
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Stopping the outlet of the column.
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Filling liquid in the bottomof the column
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Pouring gell slurry to the column.
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Settling of gels.
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Adjusting the adaptor
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Fixed adaptor
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Conneting a lineto the adaptor
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Adaptor touching the upper layer of gels.
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Gel level lowers due tothe solution flowinto the column.
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Stopping the outletline
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Connetion of pumpline to the adaptor
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Blue dextran solution flows in the column.
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2. Valve control
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Injection of sample
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FPLC controller.
3. Remote controller
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Pump front panel.
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Pump flow ratecalibration
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Manual mode of gradient setting
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Valve number setting
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Flow rate setting
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Multimeter for digital data aquisition
4. Multimeter
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UV detector
5. Detector
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Software UV signaldata aquisition
6. Software(PROTEK 506)
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Multimeter settingfor sending data to notebook computer
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Sampling time setup
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File name.
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Save data file In a folder
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Starting data acquisitionEnd ing data collection