MFSH MFSH 20032003
PRESS F1F1 FOR GUIDEANCE
Staphylococcus & StreptococcusStaphylococcus & StreptococcusStation 1: Staphylococcus VS. Streptococcus 3Staphylococcus (aureus & epidermidis)Staphylococcus (aureus & epidermidis) 44Station 2: Gram stain of Staphylococcus 5Station 3: Gram stain of staphylococcus (pus) 6Station 4: Staphylococcus growth on blood agar 7Station 5: Deoxyribonulease test 8Station 6: Mannitol sugar fermentation test 9Station 7: Coagulase test 10Station 8: Antibiotic sensitivity test, treatment & diseases 11Station 9: Summary and revision 12StreptococcusStreptococcus 1313Station 10: Gram stain of Streptococcus 14Station 11: Gram stain of Streptococcus (pus) 15Station 12: Gram stain of Streptococcus pneumoniae 16Station 13: Gram Stain of Streptococcus pneumoniae 17Station 14: Capsule stain of Streptococcus pneumoniae 18Station 15: Alpha heamolytic Streptococci 19Station 16: Beta heamolytic Streptococci 20Station 17: Gama or non-heamolytic Streptocooci 21Station 18: Optochin test 22Station 19: Bacitracin test 23Station 20: Capsule of Streptococcus Pyogens 24Station 21: Lancefield Grouping of beta heamolytic Streptococci 25Station 22: Treatment & diseases 26Station 23: Summary and revision 27
Staphylococcus StreptococcusSaline SalineH2O2 H2O2H2O + O2 (bubbles) H2O2
1) Bring 2 slides
2) Add saline
3) Put some of the bacteria
4) Add H2O2
5) See the difference
6) Take a look on the movie “Staph Vs Strept” included in the cd
Station 1Station 1
Station 2Station 2
Station 3Station 3
StaphylococciStaphylococci
Pus cellsPus cells
Station 4Station 4
اللون يكون اللون المفروض يكون المفروضللصفار للصفار مائل مائل
“ وقتها ” تأخذ لم لكن “ و وقتها ” تأخذ لم لكن و
يكون يكون المفروض المفروض F بياضا أكثر F اللون بياضا أكثر اللون
. الموضح .من الموضح من
تأخذ ” لم لكن تأخذ ” و لم لكن ووقتها“ وقتها“
Station 5Station 5
Station 6Station 6 MSA = Mannitol sugar agar
Color not changedColor not changedColor changed to Color changed to yellowyellow
Station 7Station 7
Liquid state Coagulated state
Station 8Station 8
Not all the members of the same Not all the members of the same species share all properties species share all properties together. For example, not all together. For example, not all staphylococcus aureus are staphylococcus aureus are sensitive to the same antibiotics. sensitive to the same antibiotics. (some of them are resistant to (some of them are resistant to penicillin and others are not).penicillin and others are not).
Bacteria
Control
When the distance between the control and the antibiotic is shorter than or equal to the distance between the antibiotic and the bacteria.
A =< B
The bacteria is called sensitive “to that anti-biotic”
When the distance between the control and the antibiotic is longer than the distance between the antibiotic and the bacteria.
A > B
The bacteria is called resistant “to that anti-biotic”
Sensitive
Sensitive Resistant
Sensitive
Some “tribes” of Staphylococcus areus are sensitive to Penicillin (also Cloxacilin, Methicillin…) while others are not.
Staph.aureus
Infections:-
1- Pyogenic infections and abscesses
2- Osteomylitis
3- Food poisoning “milk products” vomiting
4- Toxic shock syndrome in young women
5- SSSS “Staphylococcus Scalded Skin Syndrome”
Treatment:-
Drug of choice “Cloxacilin”
if MRSA, (beta-lactamase producers) then we use “Vancomycin”
Staph.epidermidis & Staph.saprophyticus
Infections:-
Epidermidis endocarditis with patients with catheters, cannulae & other artificial objects.
Saprophytics Urinary tract infections in young female
Treatment:-
“Vancomycin”
Station 9Station 9
Gram stainGram stain
Color on Blood Color on Blood agaragar
Mannitol Mannitol
fermentation testfermentation test
DNAse testDNAse test
Coagulase testCoagulase test
CatalaseCatalase
+ve +ve
Yellowish Whitish
+ve “yellow” -ve “red”
+ve -ve
+ve -ve +ve +ve
Station Station 1010
Station Station 1111
Station Station 1212
Station Station 1313
Capsule
(transparent)
Organism (red)
Station Station 1414
Streptococcus can be divided upon their Streptococcus can be divided upon their hemolytic activity into 3 groups:hemolytic activity into 3 groups:
1)1)αα Hemolytic (e.g. Streptococcus Hemolytic (e.g. Streptococcus pneumoniae)pneumoniae)
2)2)ββ Hemolytic (e.g. Streptococcus pyogenes) Hemolytic (e.g. Streptococcus pyogenes)
““These are also grouped by the These are also grouped by the LACEFIELDLACEFIELD grouping {from A-H, K-W} as you will see grouping {from A-H, K-W} as you will see later” later”
3)3)γγ or non Hemolytic (e.g. Streptococcus or non Hemolytic (e.g. Streptococcus mutans)mutans)
Station Station 1515
Station Station 1616
Station Station 1717
No colonies No colonies around the around the OPTOCHIN OPTOCHIN
There are There are colonies around colonies around the OPTOCHIN the OPTOCHIN
Station Station 1818
There will be some There will be some colonies around the colonies around the BACITRACIN strip.BACITRACIN strip.
Station Station 1919
Upon the Lancefield grouping
Station Station 2020
Station Station 2121
Station Station 2222
Alpha hemolytic streptococci (pnemococcus)
Infection:-
acute pnemonia
sinusitis
otitis media
meningitis
Treatment:-
“Penecillin”
Vaccination:
By the Polysaccharide capsule
Beta hemolytic streptococci gr.A (pyogens)
Infection:-
pharyngitis (sore throat)
tonsilitis
Treatment:-
“Penecillin”
Some notes:Some notes:
•All Staph. :- All Staph. :- VancomycinVancomycin But in But in non MRSAnon MRSA:- :- CloxacilinCloxacilin is better is better
•All Strept :- All Strept :- PenecilinPenecilin
Gram stainGram stain
Color on Blood agarColor on Blood agar
Color on ChocolateColor on Chocolate
Hemolytic activityHemolytic activity
Lancefiel GroupingLancefiel Grouping
Optochin testOptochin test
Bacitracin testBacitracin test
CatalaseCatalase
+ve +ve +ve Green Clear No Chg. Green No Chg. No Chg. Alpha Beta Non
+ve -ve -ve -ve +ve -ve -ve -ve -ve
Station Station 2323
NO Gp.A NO
يا يا دعواتكم دعواتكم
شبابشباب
A word can change the outcome of A word can change the outcome of
many things. So, if you either liked or many things. So, if you either liked or
didn’t like this project, why keep your didn’t like this project, why keep your
thoughts to yourself ?thoughts to yourself ?
Send us your comments and ideas Send us your comments and ideas
to to [email protected], It will
mean too much to us, “even if it was just “even if it was just
one word”one word”
THANXTHANX
M. F. ShaheenM. F. Shaheen 2003 2003
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