Download - ACVP - Jackson Laboratory Report - c.ymcdn.comc.ymcdn.com/.../Externship_Reports/ACVP_-_Jackson_Laboratory_Re.pdfwork for Dr. John Sundberg in his lab working on ... , and SWR/J strains.

SarahLinnTheOhioStateUniversity

TheJacksonLaboratory–BarHarbor,Maine FromSeptember18thtoOctober13th,2017,IwasabletoworkforDr.JohnSundberginhislabworkingondermatopathologyresearchatTheJacksonLaboratoryinBarHarbor,Maine.Dr.Sundbergiswellknowninthefieldandknowledgeableaboutthevariouslaboratorymousestrains.Myprojectwasaretrospectiveanalysisofmousenailslideswithinapreviouslypublishedlargescaleaging

studyfrom2007.Thisstudylookedatdiseasesof30agingmicestrainsfromTheJacksonLaboratoryat12and20monthsandalsoduringalongitudinalstudy.Dr.SundbergnoticedthattheNON/ShiLtJmicehadahighprevalenceofdystrophicnaillesionsalongwithsomeotherstrainsbutatlowerrate.HehopedtocharacterizethelesionfurtherandkepttheslidesuntilAllisonMustonenandIcameforourexternships.Analysisoftheslidesshowednaildystrophy,subungualinflammation,remodelingofthedistalphalanx,hyperplasiaofthenailmatrixandnailbed,andoccasionallyprotrusionofthedistalphalanxthroughthehyponychium.ThelesionswerealsoseenintheC57BR/cdJ,C57BL/6J,FVB/NJ,KK/H1J,MRL/MpJ,NZO/H1LtJ,NZW/LacJ,SM/J,andSWR/Jstrains.WegatheredsamplesfromyoungerNON/ShiLtJbreederstoseeifthelesionwaspresentatearlierageswhichthereweresomemicethatwereaffected.Completeanalysisoftheslidesallowedustoproposeaspecificpathogenesisforthislesionwhichweareaimingtopublishlaterthisyear. Duringtheslideanalysis,AllisonandIdiscoveredthatabout40%ofslideswerenotdiagnosticforustoevaluatethenails.Mousenailsarehardtosectionbecauseoftheirsmallsize,varyingtissueswithinthenailunit,anddifficultyorientingthefeetwithinparaffin.Ourotherprojectwastodevelopabettermeansofsectioningmousenailsinordertoevaluatestrainsforphenotyping.WeworkedwiththehistologytechniciansatTheJacksonLaboratorytoseewhatfixativesanddecalcificationprotocolswouldworkbestforsectioning.StartingwithC57BL/6Jfemalesofthesameage,wedevelopedaprotocolthatworkedwellforsectioningthetissues,orientingthenailsinwax,andimmunohistochemicalstainingoftheslides.Wehopetopublishourresultsafterrepeatingthemethodsforvariousstrainsandmalesversusfemales. Asidefromtheseprojects,Iattendedthe16thAnnualWorkshoponthePathologyofMouseModelsofHumanDisease.Iwasabletonetworkwithleadersinthefieldofmousepathologyandmakevaluableconnectionsformycareer.AllisonandIalsopresentedourworkattheconferencewhichwaswellreceivedbytheattendees. Overall,IhadavaluablecareerexperiencewithDr.Sundberg.HewasagreatmentorthroughouttheexternshipandtaughtmewhataveterinarypathologistcanachieveataninstitutionlikeTheJacksonLaboratory.ItrulyappreciateACVP’sfinancialassistanceformyexternshipandamthankfulforthetrainingIreceived.