International Journal of Trend in
International Open Access
ISSN No: 2456
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Effect of Glucose Bacterial Isolates
LecturerShri Shivaji Science College Nagpur
ABSTRACT The demand for biosurfactants is gradually increasing and are thus substituting their chemically synthesized counterparts [14]. The production of biosurfactants commercially requires high expenses. For the production of biosurfactant proper optimization ofphysico-chemical parameters is very important. Hence the research was conducted to study the effect of glucose as a carbon source for production of biosurfactant using bacterial isolates from oil contaminated sites in MSM medium. KEY WORDS: Biosurfactants, BacterGlucose medium, Minimal Salt Medium. INTRODUCTION Biosurfactants are amhiphatic compoundsmostly produced by bacterial speciesBiosurfactants are reported to be toxic in natureThey are biodegradable in nature as compared to other commercially available surfactants [1]. Biosurfactantshave varied environmental applicationshydrocarbon remediation from soil, dispersionspills and enhancement of oil recovery processes5]. Biosurfactants have potential use in industry, agriculture, pharmaceutics, petropaper and pulp industry etc. and therefore research in the area of biosurfactants has expanded quite a lot in recent years. The development of this line of research is of great importance, mainly in view of the present concern with protection of the environment
International Journal of Trend in Scientific Research and Development (IJTSRD)
International Open Access Journal | www.ijtsrd.com
ISSN No: 2456 - 6470 | Volume - 3 | Issue – 1 | Nov
www.ijtsrd.com | Volume – 3 | Issue – 1 | Nov-Dec 2018
f Glucose on Biosurfactant Production Isolates from Oil Contaminated Sites
Anjali Sharma ecturer, Department of Microbiology,
Shri Shivaji Science College Nagpur, Maharashtra, India
The demand for biosurfactants is gradually increasing and are thus substituting their chemically synthesized counterparts [14]. The production of biosurfactants commercially requires high expenses. For the production of biosurfactant proper optimization of the
chemical parameters is very important. Hence the research was conducted to study the effect of glucose as a carbon source for production of biosurfactant using bacterial isolates from oil
Biosurfactants, Bacterial isolates, Glucose medium, Minimal Salt Medium.
hiphatic compounds that are bacterial species[1,4,17].
toxic in nature [7]. biodegradable in nature as compared to other
surfactants [1]. Biosurfactants environmental applications such as
hydrocarbon remediation from soil, dispersion of oil enhancement of oil recovery processes [2,
potential use in various petro chemistry,
therefore research in expanded quite a lot in of this line of research
view of the present concern with protection of the environment. The most
significant advantage of a biosurfactant oversurfactants is its ecological acceptancthe other advantages of biosurfactants includeselectivity, specific activity at extreme temperatures,pH and salinity and the possibility of their productionthrough fermentation [9, 10].biosurfactants have been reportbiosurfactant is influenced by theenhance the growth of the microbe.to stimulate biosurfactant productionoptimization of ecological, physiologicalnutritional conditions [15]. MATERIALS AND METHODS:In order to study effect of glucose as a carbon source on production of biosurfactant, Mwas used as the basal medium.the glucose was kept at 1% (w/v). 1% inoculum culture of isolated bacterial IHD19, IHD21, IHD36, IHD44, IHD58, IHD80, IHD89, IHD96, IHD112, IHD 148, IHD152, IHD157, IHD 176, IHD178, and IHD188) were100 ml of Minimal Salt Medium containing glucose as carbon source and were incubated in rotarat 120rpm at 37 ⁰C for 7 days. Growth of cells was monitored by measuring the absorbance (Optical Density), A540. The biosurfactant production was determined by measuring the EI% as described under[2, 12, 13, 16, 18]
Research and Development (IJTSRD)
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1 | Nov – Dec 2018
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n Biosurfactant Production using rom Oil Contaminated Sites
significant advantage of a biosurfactant over chemical is its ecological acceptance [6,8]. Some of
the other advantages of biosurfactants include selectivity, specific activity at extreme temperatures, pH and salinity and the possibility of their production through fermentation [9, 10]. A large variety of biosurfactants have been reported [11]. Production of biosurfactant is influenced by the factors which also enhance the growth of the microbe. One strategy used to stimulate biosurfactant production has been the optimization of ecological, physiological and
MATERIALS AND METHODS: glucose as a carbon source
on production of biosurfactant, Minimal Salt Medium as the basal medium. The concentration of
was kept at 1% (w/v). 1% inoculum strains (IHD3, IHD13,
IHD19, IHD21, IHD36, IHD44, IHD58, IHD80, IHD89, IHD96, IHD112, IHD 148, IHD152, IHD157, IHD 176, IHD178, and IHD188) were inoculated into
edium containing glucose were incubated in rotary shaker
C for 7 days. Growth of cells was monitored by measuring the absorbance (Optical
. The biosurfactant production was determined by measuring the EI% as described under
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
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RESULTS AND DISCUSSION: Table.1. Effect of Glucose as Carbon Sources
pH = 7.00, Temperature: 37 ⁰C. Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
Bacterial
isolates
IHD 3
IHD 13
IHD 19
IHD 21
IHD 36
EI % 64 78 88 69 76 O.D
600 nm 0.7 1.6
1.82
0.8 1.76
A number of carbon sources have been used by many researchers for biosurfactant production. The quality and quantity of produced biosurfactant are affected and influenced by the nature of the carbon substrate. Out of the various carbon sources screened forproduction of biosurfactant in the current study, glucose was found to be the best carbon source for growth and biosurfactant production. The highest Emulsification activity was obtained using glucose at concentration of (1% w/v) as source of carbon alongwith engine oil, resulted in higher emulsifier activity (EI% = 88 % and EA = 1.82). The results were in disagreement with the founding of A. Khopade et.al.,[11] where they obtained maximum activity (EI%= 80%) when using sucrose as source of carbon along with engine oil by marine isolate sp. B3. Maximum growth of bacterial cell and biosurfactant production by bacterial species isolated from petroleum contaminated soil were observed by using glucose as the source of carbon along with engine oil where the maximum value of emulsification index
64
78
88
6976
0
10
20
30
40
50
60
70
80
90
100
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
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Table.1. Effect of Glucose as Carbon Sources Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
shaking speed = 125 r.p.m. IHD 44
IHD 58
IHD 80
IHD 89
IHD 96
IHD
112
IHD
148
IHD
152 68 75 67 54 66 76 62 73 1.28
1.5 1.07
0.62
0.9 0.6 0.4 0.7
A number of carbon sources have been used by many researchers for biosurfactant production. The quality and quantity of produced biosurfactant are affected and influenced by the nature of the carbon substrate. Out of the various carbon sources screened for production of biosurfactant in the current study, glucose was found to be the best carbon source for growth and biosurfactant production. The highest Emulsification activity was obtained using glucose at concentration of (1% w/v) as source of carbon along with engine oil, resulted in higher emulsifier activity (EI% = 88 % and EA = 1.82). The results were in disagreement with the founding of A. Khopade
where they obtained maximum activity (EI%= 80%) when using sucrose as source of carbon along with engine oil by marine isolate Streptomyces
Maximum growth of bacterial cell and biosurfactant bacterial species isolated from
were observed by using glucose as the source of carbon along with engine oil where the maximum value of emulsification index
was found to be EI%= 88%. which was given by IHD 19, Intermediate range of Emulsification Index were recorded between 79% to 64% given by the bacterial isolates viz.,. IHD 3, IHD 13, IHD 21, IHD 36, IHD 44, IHD 58, IHD 80, IHD 96, IHD 112, IHD 148, IHD 152, IHD 157, IHD 176. IHD 178 and IHD188. Lowest EI% was recorded to be 54% which was given by IHD 89. CONCLUSION: Growth and production medium for biosurfactant production was optimized by using glucose as the carbon source in the Minimal Salt Medium.Maximum production of biosurfactant was observed in MSM medium containing glucose and 2% engine oil. REFERENCES: 1. Ainon, H., Noramiza, S. and Shahidan, R. (2013).
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Effect of Glucose
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
Dec 2018 Page: 731
Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
IHD
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IHD
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IHD
178
IHD
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0.8 1 0.6 0.9
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Growth and production medium for biosurfactant production was optimized by using glucose as the carbon source in the Minimal Salt Medium. Maximum production of biosurfactant was observed in MSM medium containing glucose and 2% engine
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International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
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International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
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