0

50

100

150

200

250

300

350

Se

cre

ted

PC

SK

9 (

ng

/ml)

WT V5 QH V5

ng Input DNA 125 250

ng non-relevant DNA375 250 0 375 250 0

WT NT

375 250 0

Input DNA

500 125 250 500 125 250 500

Supplemental Figure 1

ELISA demostrating the linearity of WT PCSK9 Secretion from HuH7 cells transfected with WT PCSK9 untagged (WT NT) and V5-tagged WT PCSK9 (WT V5) but no secretion with Q152H PCSK9 (QH V5), with amounts of cDNA as shown. n=3; ave SEM. HuH7 cells were grown at 37C in DMEM + 10% FBS + gentamycin (28 g/ml). Cells (3X105) were transiently-transfected with cDNA encoding human PCSK9 (pIRES2) using Effectene protocol from Qiagen 24 hours following plating.