Zebrafish
Transcript of Zebrafish
ASR Zebrafish Services
• Fish Housing and Care
• Embryo Production
• Genotyping
• Manipulation of Gene Expression
- Morpholino, RNA, and DNA microinjections
• Toxicity and Drug Screening
• Phenotypic Analysis
- Visualization, Documentation and Interpretation
• Consulting and Training
Manipulation of Gene Expression in Zebrafish
RNA
Morpholino(MO)
(knockdown)
cDNA
(transient& mosaic)
cDNA (stabletransgenic)
Phenotype analyses in zebrafish
• Morphology and/or histology
•whole-mount in situ hybridization histochemistry (ISHH)
• whole-mount immunohistochemistry (IHC)
• fluorescent marker (EGFP) expression (living embryos)
• acridine orange apoptosis assay (living embryos)
• cell transplantation and lineage tracing (living embryos)
Toxicity and drug screening in zebrafish
96 or 384 well format
wt Ezrin MO1 wt NCS305787
wt NSC668394 wt NSC668394
Novel compound activity assay
Small compound library screening
Transgene based assays
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01
In your publications, remember to mention support provided from the GHUCCTS.