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Your tool for high resolution EM

Are you looking for a secondaryantibody conjugated to theoptimal size gold particle?

We offer over 100 AbGold conjugatedsecondary antibodies for all yourelectron microscopy imaging needs.

Discover more at abcam.com/AbGold

168_12_JL

Discover more at abcam.com/AbGold

Abcam gold-conjugated secondaries for superior EM results

Gold particles can be used to label primary antibodies for identifying surface antigens in direct assays.However, they are mainly used as secondary antibody labels in applications such as TEM and SEM.

Target species Purification 1.4 5 6 10 12 15 18 20 25 30 40

Chicken IgY Affinity purified - - - - - - - - - - -Pre-adsorbed - - - - ab105266 - ab105267 - - - -

Goat IgG Affinity purified - - - ab41496 - - - - - - -Pre-adsorbed - - ab105268 - ab105269 - ab105270 - ab41510 - -

Guinea-pig IgG Affinity purified - - - - - - - - - - -Pre-adsorbed - - ab105271, ab105273 - ab105272 - - - - - -

Mouse IgG Affinity purified - - ab39616 ab39593 - - - - - - -Pre-adsorbed - - ab105276 - ab105277 - ab105278 - - - -

Rabbit IgG Affinity purified - - - ab39597 - - - ab41514 - -Pre-adsorbed - - ab105294 - ab105295 - ab105296 - - - -

Sheep IgG Affinity purified - - ab39602 - - - - - - - -Pre-adsorbed - - ab105307 ab39611 ab105308 - ab105309 - - - -

Key: EM EM, WB ELISA, EM ELISA, EM, WB EM, IF, WB

ELISA Enzyme-linked immunosorbent assayEM Electron microscopyIF Immunofluorescence

SEM Scanning electron microscopyTEM Transmission electron microscopyWB Western blotting

Abbreviations

Target species Purification 1.4 5 6 10 12 15 18 20 25 30 40

Chicken IgY Affinity purified - - ab39604 ab41511 - - - - ab41494 - -Pre-adsorbed - - - - - - - - - - -

Guinea-pig IgG Affinity purified ab30827, ab30828, ab81402 - - - - - - - - - ab33108Pre-adsorbed - - ab39617 - ab81398 - - - - -

Human IgG Affinity purified ab30825, ab30826 - ab39618 ab39596 - ab81430 - - - - -Pre-adsorbed - - - - ab105274 - ab105275 - - - -

Mouse IgG Affinity purified ab30812, ab33109 - ab39614, ab41499 ab39619 - - ab105281, ab105284, - - - - ab105287Pre-adsorbed - ab27233, ab27244 ab105279, ab105282, ab27230, ab27241 ab105280, ab105286, - - ab27242 - - - ab105285 ab105289

Rabbit IgG Affinity purified ab30814,ab30815, - ab39595, ab41498 ab39619 - - - - - - - ab33110 Pre-adsorbed - ab27235, ab27240 ab105297 ab27234, ab27239 ab105298 ab27236 ab105296 ab27237 - ab119178 ab119180

Rat IgG Affinity purified ab30818 - - ab81409 - ab81410 - - ab41513 - -Pre-adsorbed - - ab105300 - ab105304 - ab105305 - - - -

Sheep IgG Affinity purified - - - - - - - - - - -Pre-adsorbed - - - - - - - - - - -

Target species Purification 1.4 5 6 10 12 15 18 20 25 30 40

Chicken IgY Affinity purified - - ab39607 ab39612 - ab41500 - - - - -Pre-adsorbed - - - - - - - - - - -

Goat IgG Affinity purified ab30817 - ab39610 ab27245, ab39594 - ab27247 - - - - -Pre-adsorbed - - - - - - - - - - -

Sheep IgG Affinity purified ab30824 - - ab39609 - ab41495 - - - - - Pre-adsorbed - - - - - - - - - - -

Raised in Rabbit

Raised in Goat

Raised in DonkeyGold particle size (nm)

Gold particle size (nm)

Gold particle size (nm)

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Gold particle size Application

Ultra-small - e.g. 1.4nm (Image 1) TEM, IF, IHC (with light microscopes), standard immune TEM, WB

Small - 2nm-15nm (Image 2) ELISA, TEM, IHC, Light Microscopy, WB

Medium - 20nm-60nm SEM, TEM

Large - 80nm-250nm Flow Cytometry

Unsure about which gold particle sizeis most suitable for your experiment?

Ultra-small gold particles:We recommend using our 1.4nm gold-conjugated secondary antibodies for IHC andIF experiments as the reduced size leads to increased tissue and cell penetration.

Then have a look at our gold particle size guide:

TEM image (magnification 15,000x) of OsO4-fixed rat brain sections.The K+ channel Kv2.1 subunit was immunolabelled using a goat F(ab)anti-rabbit IgG - H&L antibody conjugated to 1.4nm gold particles.Reprinted from Neuroscience, Vol. 84, No. 1, Du, J., Tao-Cheng, J.-H.,Zerfas, P., McBain, C. J., the K+ channel, Kv2.1, is apposed to astrocyticprocesses and is associated with inhibitory postsynaptic membranes inhippocampal and cortical principal neurons and inhibitory interneurons,pp. 37-48, Copyright (1998), with permission from Elsevier.

Somatostatin28 reactivity in D cell from guinea pig pancreas. On gridimmunogold procedure using ab22682 (1:2000) and 10nm goldparticles. This image is courtesy of an anonymous Abreview.

Image 1 Image 2