Yersinia pestis Plague - pattyphdepi27 · Yersinia pestis Plague County of Los Angeles Patricia...
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Yersinia pestis
Plague
County of Los Angeles
Patricia Bolívar MS., CLS, PHM *1
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Pathogenic Yersiniae
Y. pestis – etiologic agent of plague (Black Death), obligate parasite
Y. pseudotuberculosis – primarily causes enteric disease, usually water borne, rarely food borne
Y. enterocolitica – causes food poisoning, can be food or water borne
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Types of Plague
1. Bubonic (Lymphatic system)
2. Septicemic (Blood)
3. Pneumonic (Respiratory)
4. Enteric (Gastrointestinal)
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3 Biotypes (biovars) of Y. pestis
1. Antiqua, mediaevalis, and orientalis
2. Recognized on the basis of conversion of
nitrate to nitrite and glycerol fermentation
3. 3 biotypes exhibit no difference in
virulence or pathology in animals and
humans
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3 Biotypes (biovars) of Y. pestis
Biotype
Nitrate
Nitrite
Glycerol
Fermentation
antiqua + +
orientalis + -
mediaevalis - +
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Virulence Factors of Yersinia pestis
1. LPS endotoxin – causes endotoxic shock and
death
2. F1 Antigen (caf1) – Glycoprotein envelope
antigen, antiphagocytic
3. Phospholipase D (ymt) – a.k.a. Murein toxin;
important for the colonization in the flea gut
4. Yersinia outer proteins (YOPs) – inhibit
phagocytosis and platelet aggregation
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Epidemiology of Plague Most commonly transmitted to humans by
bites of infected rodent fleas
Enzoonotic infection of rodents on every
populated continent except Australia
Direct infection by handling infected animals
Bacteria enters through breaks in skin
Inhaling respiratory droplets from plague-
infected animals or humans
Close contact < 6 feet
Xenopsylla chepsis (oriental rat flea) engorged with
blood
*2
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Epidemiology of Plague
**3
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Clinical Symptoms of the
Plague
Chills, high fever
Lymphadenopathy
Sepsis syndrome
Acute pneumonitis *4
*5
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Acceptable Specimen
Sources:
1. Direct specimen smear
2. Blood culture
3. Lymph node aspirate (bubo)
4. Respiratory culture (sputum, bronchial wash)
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Gram Stain:
Plump, gram-negative rods, 1-2 µm X 0.5
µm, that are seen mostly as single cells or
pairs and short chains.
*6
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Other stains:
Wright-Geimsa or Wayson Stains reveal the bipolar
staining characteristics of Y.pestis; closed “safety-pin” appearance
*7
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Colony Morphology:
24 hours – gamma-
hemolytic, gray-white,
translucent, pin point
colonies
48 hours - gray-white
to slightly yellow,
opaque, 1-2 mm
*8
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Colony Morphology (cont.):
At 48-72 hours,
colonies have a
raised, irregular,
“fried egg” or a
“hammered copper”
appearance
*9
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Growth:
Incubation temperature: 28°C (optimal), 35°-37°C (grows more slowly)
Atmosphere: ambient or 5% CO2
Colonies may not be visible on MacConkey (MAC) or eosin methylene blue agar (EMB) at 24 hours
MAC: small lactose negative colonies
Brain Heart Infusion Broth: flocculent clumps, “stalactites” on side and bottom of tubes
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Biochemical Reactions: Catalase – positive
Oxidase – negative
Urease – negative (rarely, strains may be positive)
Indole – negative
Triple Sugar Iron Agar (TSI) – Alkaline slant/ acid
butt (K/A) without gas or H2S
Lack the ability to ferment most carbohydrates
They primarily utilize glucose and mannitol for
energy
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Selective Media
CIN Stands for “Cefsulodin - Irgasan -
Novobiocin” Also known as “Yersinia
Selective Agar”
Characteristic “bulls-eye” colony which
has a colorless, translucent outer zone
with a red center
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CIN agar
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Other bacteria on CIN Agar
Citrobacter species, Enterobacter
agglomerans, Serratia liquefaciens, Y.
frederiksenii, Y. intermedia, and Y.
kristensenii may grow on CIN Agar and
resemble Y. pestis ("bull's-eye" colony
morphology), but are easily
differentiated by biochemical tests.
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R & F® Yersinia pestis
Chromogenic Agar (YpCM
Able to easily differentiate Yersinia
pestis from Yersinia enterocolitica,
Yersinia pseudotuberculosis, Yersinia
kristensenii, Yersinia intermedia and
most other enteric bacteria
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Yersinia pestis on
Chromogenic Agar
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Additional testing
DFA
Polyclonal rabbit anti-Y. pestis F1 antigen antibody labeled with
fluorescein isothiocyanate (FITC) reacts with specimens to
determine whether Y. pestis is present
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Reference Laboratory Procedure
Specific bacteriophage lysis test for Y. pestis
Susceptibility to lysis by the Yersinia pestis-specific
Bacteriophage Impregnated Filter Strips at 25°C and 35°C,
along with culture characteristics, can be used to confirm the
identification of Yersinia pestis.
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Etest
Y. pestis isolates tested for susceptibility to
ciprofloxacin, gentamicin, levofloxacin, and
tetracycline.
Etest directly quantifies antimicrobial
susceptibility in terms of MIC (µg/ml) values
Mueller-Hinton agar plates (M-H), (4 mm
depth), 150 mm plates
0.5 McFarland turbidity
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References Information and pictures from:
Center of Disease Control (CDC), “Level A Laboratory Procedures for
Identification of Yersinia pestis,” Laboratory Response Network( LRN),
13 Dec 2001, <http://www.lrnb.cdc.gov>
1ZKEA, “Bubonic Plague,” Biological Terrorism: Emerging Diseases,
<http://www.zkea.com/archives/archive02005.html>
3Kirksville College of Osteopathic, “Epidemiology of Plague,”
Lymphoreticular and Hematopoetic Infections,3 Aug 2004,
http://www.kcom.edu/faculty/chamberlain/Website/lectures/lecture/p
lague.htm
Clinical and Laboratory Standards Institute. Jan. 2009. Performance
Standards for Antimicrobial Susceptibility Testing; Nineteenth
Informational Supplement. M100-S19. Vol. 29, No. 3. Wayne, PA.