Xxxxx

17
MULTI-DESARROLLO-HPTLC MÉTODO PARA LA CUANTIFICACIÓN DE LA HIOSCIAMINA, ESCOPOLAMINA Y SUS PRECURSORES BIOSINTÉTICOS EN SELECCIONADAS PLANTAS SOLANÁCEAS CULTIVADAS EN CONDICIONES NATURALES Y COMO CULTIVOS IN VITRO Equipo: Aguilar Magaña Diana Canto Rodríguez Yuseline Pérez Muñoz Sharon

Transcript of Xxxxx

Multi-developmentHPTLC Method for Quantitation of Hyoscyamine, Scopolamine and their Biosynthetic Precursors in Selected Solanaceae Plants Grown in Natural Conditions and as In Vitro Cultures

Multi-desarrollo-HPTLC Mtodo para la cuantificacin de la hiosciamina, escopolamina y sus precursores biosintticos en seleccionadas plantas solanceas cultivadas en condiciones naturales y como cultivos in vitroEquipo:Aguilar Magaa DianaCanto Rodrguez YuselinePrez Muoz SharonTropano alcaloides El objetivo de esta investigacin fue desarrollar las condiciones para el TLC, separacin y cuantificacin de hiosciamina , escopolamina, Littorine, anisodamina y cuscohigrina encontrado en los extractos de las plantas intactas y/ o in-vitro biomasas deHyoscyamus niger, Atropa baetica , Atropa belladona y Datura stramonium.

Productos qumicos y normas experimentalesMaterial vegetal Preparacin de la muestra

Laliofilizacines la remocin de agua de las clulas congeladas (slidas) a presin reducida (sublimacin). Para llevar a cabo este procedimiento, se toma una muestra del cultivo por conservar y se suspende en algn medio con crioprotectores como leche, suero o glutamato de sodio.6Cromatografa

8Las placas de TLC cargadas se desarrollaron utilizando una de dos etapas multi-desarrollo (MD) , con cada etapa que comprende dos pasos.Los otros compuestos fueron examinados en = 520 nm despus de la pulverizacin y derivatizacin con Reactivo de Dragendorff siguiendo una modificacin.Los cromatogramas fueron documentados fotogrficamente.Las exploraciones lineales se obtuvieron con hendidura dimensiones de 0,02 mm X 10 mm.Munier y Macheboeuf (Baerheim-Svendsen y Verpoorte, 1983).

9 Validacin del mtodo

Medicin de contenido

Resultados y discusiones

12REFERENCIASJaremicz, Z., Luczkiewicz, M., Kisiel, M., Zrate, R., Jaber-Vazdekis, N., Migas, P. Multi-development-HPTLC Method for Quantitation of Hyoscyamine, Scopolamine and their Biosynthetic Precursors in Selected Solanaceae Plants Grown in Natural Conditions and as In Vitro Cultures. Phytochemical Analysis. 2013


B R EPORT XXXXX Company Name Here XXXXX BEST’S … · Page 1 of 24 Printed June 15, 2018 B EST’S R ATING R EPORT XXXXX Company Name Here XXXXX. B. EST’S . R. ATING. R. EPORT.

B R EPORT XXXXX Company Name Here XXXXX BEST’S … · Page 1 of 24 Printed June 15, 2018 B EST’S R ATING R EPORT XXXXX Company Name Here XXXXX. B. EST’S . R. ATING. R. EPORT.

vSphere - msmhec.plmsmhec.pl/vmw/Documents/S_Tasks.pdf · serialnum --esx=XXXXX-XXXXX-XXXXX-XXXXX-XXXXX rootpw XXXXXXXXXXXXX autopart --firstdisk --overwritevmfs install cdrom

vSphere - msmhec.plmsmhec.pl/vmw/Documents/S_Tasks.pdf · serialnum --esx=XXXXX-XXXXX-XXXXX-XXXXX-XXXXX rootpw XXXXXXXXXXXXX autopart --firstdisk --overwritevmfs install cdrom

RCRMC WLAN 4TH FLOOR Arch E - Purchasing > Home...Fourth Floor BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx 4CB 4CA E 2 3 1 E 3 2 E 2 E 3 2 E 3 5 5 3 4 4 4 4CB.701 4CB.702

RCRMC WLAN 4TH FLOOR Arch E - Purchasing > Home...Fourth Floor BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx BS-xxxxx 4CB 4CA E 2 3 1 E 3 2 E 2 E 3 2 E 3 5 5 3 4 4 4 4CB.701 4CB.702

[XXXXX] Standard Consultant Contract For Local … D to I.M. 3.305 May 26, 2017 Page 1 of 36 Contract No. [XXXXX] Owner Project No. [XXXXX] Iowa DOT Project No. [XXXXX] Standard Consultant

[XXXXX] Standard Consultant Contract For Local … D to I.M. 3.305 May 26, 2017 Page 1 of 36 Contract No. [XXXXX] Owner Project No. [XXXXX] Iowa DOT Project No. [XXXXX] Standard Consultant

XXX,XXX SF @ XX' XXXXX XXXXX XXXXXX · Ceiling Height XX' Xxxxx XX' Xxxxx (XX%) XX' Xxxxx (XX%) Dock Doors X Xxxxx (xxxxxxx) XX Xxxxx X Xxx Xxxxx (xxxxxxx) X Xxxxxx Column Spacing

XXX,XXX SF @ XX' XXXXX XXXXX XXXXXX · Ceiling Height XX' Xxxxx XX' Xxxxx (XX%) XX' Xxxxx (XX%) Dock Doors X Xxxxx (xxxxxxx) XX Xxxxx X Xxx Xxxxx (xxxxxxx) X Xxxxxx Column Spacing

User Manual 5 - B&H PhotoIn the last field: Serial Number (XXXXX-XXXXX-XXXXX-XXXXX), if you are registering the Professional version (PRO) of the software then the Serial number will

User Manual 5 - B&H PhotoIn the last field: Serial Number (XXXXX-XXXXX-XXXXX-XXXXX), if you are registering the Professional version (PRO) of the software then the Serial number will

xxxxxxxxxx xxxxxxxxxxxxxxxxxxx xxxxx

xxxxxxxxxx xxxxxxxxxxxxxxxxxxx xxxxx

civil defense - publications.iowa.govpublications.iowa.gov/25063/1/civil defense training.pdf · CALHOUN HAMILTON H."IRDIN GRUNDY ]_xxxxx]xxxxx xxxxxxx ~ xxxxx xxxxx : \ J•• TAw.

civil defense - publications.iowa.govpublications.iowa.gov/25063/1/civil defense training.pdf · CALHOUN HAMILTON H."IRDIN GRUNDY ]_xxxxx]xxxxx xxxxxxx ~ xxxxx xxxxx : \ J•• TAw.

PCI PA DSS Implementation Guide - Verifone · 16.07.2018  · PCI PA DSS Implementation Guide: MultiPOINT 03.20.072.xxxxx, 04.20.073.xxxxx & 05.20.074.xxxxx Author Sergejs Melnikovs

PCI PA DSS Implementation Guide - Verifone · 16.07.2018  · PCI PA DSS Implementation Guide: MultiPOINT 03.20.072.xxxxx, 04.20.073.xxxxx & 05.20.074.xxxxx Author Sergejs Melnikovs

Cosmic Girl - Jamiroquai · ã.. .. 74 xxx œ xxxxx œ xx œœœœ xxx œ xxxxx œ xx œœœœœ xxx œ xxxxx œ xx œœœœ 1. xxx œ xxxxx œ xx œœœœœ.œ ã .. .. 78 2. xxx

Cosmic Girl - Jamiroquai · ã.. .. 74 xxx œ xxxxx œ xx œœœœ xxx œ xxxxx œ xx œœœœœ xxx œ xxxxx œ xx œœœœ 1. xxx œ xxxxx œ xx œœœœœ.œ ã .. .. 78 2. xxx

Rotator Cuff Anatomy Dr. XXXXX Hospital XXXXX Date XXXXX

Rotator Cuff Anatomy Dr. XXXXX Hospital XXXXX Date XXXXX

NAME OF VESSEL: xxxxx - Merex Yachts · Type: xxxxx Flag: xxxxx Length: 76' This Date: 3 November 2015 and Place: xxxxx Between the Undersigned Parties it has been Agreed as Follows

NAME OF VESSEL: xxxxx - Merex Yachts · Type: xxxxx Flag: xxxxx Length: 76' This Date: 3 November 2015 and Place: xxxxx Between the Undersigned Parties it has been Agreed as Follows

TM XXXXX-XX-XXX-XXX/XXXXX XXXX-XX-XXX-XXXX

TM XXXXX-XX-XXX-XXX/XXXXX XXXX-XX-XXX-XXXX

Egyptian mythology22222 xxxxx

Egyptian mythology22222 xxxxx

2018 NCAA MEN’S BASKETBALL TOURNAMENT xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx Xxx Xxx March Xx March Xx March Xx Thursday Xxx Friday

2018 NCAA MEN’S BASKETBALL TOURNAMENT xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx xxxxx Xxx Xxx March Xx March Xx March Xx Thursday Xxx Friday

Dated xxxxxx 2012/13 JESUS COLLEGE, OXFORD and xxxxx ...intranet.jesus.ox.ac.uk/Data/Sites/1/media/documents_public/... · JESUS COLLEGE, OXFORD and xxxxx xxxxx xxxxx xxxxx COUNTERPART

Dated xxxxxx 2012/13 JESUS COLLEGE, OXFORD and xxxxx ...intranet.jesus.ox.ac.uk/Data/Sites/1/media/documents_public/... · JESUS COLLEGE, OXFORD and xxxxx xxxxx xxxxx xxxxx COUNTERPART

HSMx-A2-xx-xxxxx Bi-Color HSMx-A3xx-xxxxx Tri-Color

HSMx-A2-xx-xxxxx Bi-Color HSMx-A3xx-xxxxx Tri-Color

XXXXXXXXXXXX XXXXXX XXXXX XXXXXX XXXX XXXXX …

XXXXXXXXXXXX XXXXXX XXXXX XXXXXX XXXX XXXXX …

Benefice of xxxxx

Benefice of xxxxx

NAME OF VESSEL: xxxxx - Merex Yachts · MYBA CHARTER AGREEMENT - E-Contract N°°265319193151103 PAGE ONE OF EIGHT NAME OF VESSEL: xxxxx Port of Registry: xxxxx Type: xxxxx

NAME OF VESSEL: xxxxx - Merex Yachts · MYBA CHARTER AGREEMENT - E-Contract N°°265319193151103 PAGE ONE OF EIGHT NAME OF VESSEL: xxxxx Port of Registry: xxxxx Type: xxxxx