www.flowcytometryservices.co.uk

History

Dr Darren Sexton (MED), Mr Geoff Carruth (REE), Dr Ailsa Harpur (REE) bid for EEDA Capital Investment for Life Sciences Competition 2008

BD Aria II Sorter purchased and housed in Biomedical Research Centre 2009

Joint Faculty of Science and Faculty of Health appointment of Dr Andrew Goldson 2009

Further investment; Accuri C6 Analyser

BD FACS Aria II:•70,000 events per second

•Sort into tubes, plates (6, 24, 48, 96, 384-wells) or onto microscope slides.

•4 lasers (488nm, 633nm, 405nm, 375nm)

•complex multi-colour analysis; 15 parameter analysis

Accuri C6•10,000 events/second

•2 lasers (blue 488nm and red 640nm)

• 6 parameter analysis

Flow Cytometry facilitates cell and particulate analysis….

•Particle size and granularity

•Fluorescent colour detection and intensity measurement

•Colour associated with probe of interest..... myriad probes

Techniques versus applications eg Annexin V/PI staining is established Viability technique

Application: Cancer drug screening, nutraceutical assessment etc

Bespoke flow cytometric sample analysis including:

Services:                               

•Cell sorting

•Cell cycle analysis

•Apoptosis analysis

•Multicolour analysis

•Functional analysis

•Stem Cell analysis

What’s the process?

Initial Enquiry quotation invoicing

•Managed consultancy - UEA Consulting Ltd

We can also suggest additional expertise and know-how linked to your needs.

•Sample Preparation?

1. You provide samples

2. We can prepare samples• Dr A Goldson or KT technician Mr Matt Jefferson

Design Analysis Interpretation

The Greater Environment

Buying into a network of expertise:

•Norwich Flow Cytometry Network• In conjunction with Dr Roy Bongaerts, Institute of Food Research

•Worldwide Flow Cytometry Network

•Core Facilities Managers consortia• Imaging and Flow cytometry

• Biosciences and Biotechnology • Health and Life Sciences

• Pharmaceutical

• Diet and Health

Industry Sectors

•Food and Drink

•Nutraceuticals

•Human, Animal and Plant Biotechnology

•Healthcare technology (eg infection control)

•Emerging stem cell applications

•Nanotechnology applications

Expertise

BMRC

Biomedical Research Centre

• £16.5M state-of-the-art infrastructure

• School of Biological Sciences and School of Medicine, Health Policy and Practice

• BMRC’s Disease Modelling Unit

• Containment Level 3 laboratory for handling pathogenic organisms

Clinical Research and Trials Unit

• £3.5M state-of-the-art infrastructure

• Phase 1 and 2 trials

• 6 bedded inpatient facility with dedicated IT access, sample preparation room, kitchen and patient toilet

• New 9 clinic room outpatient facility with shower, kitchen, sample preparation

• Archive facilities

2500 scientists

9000 staff

30 innovative companies

Research budget >£125 million p.a

Norwich is ranked 4th in the UK for the number of “most highly cited scientists”

Norwich Research Park

GSK • drug efficacy• cell signalling• redefining the role of an immune cell in disease with respect to its

progenitors

AKL• nutraceuticals in COPD• Inflammation markers in processed sputum

Some Clients

Dr Darren W SextonLecturer in Immunology & LTN Business FellowBioMedical Research CentreFaculty of HealthUniversity of East AngliaNorwichUKNR4 7TJ Tel: +44(0)1603 593321Fax: +44(0)1603 591750E-mail: d.sexton@uea.ac.uk

Contact DetailsPLEASE DIRECT ALL BOOKINGS AND BUSINESS ENQUIRES TO DR ANDREW GOLDSON IN THE FIRST INSTANCE.

Dr Andrew GoldsonFlow Cytometry ServicesBioMedical Research CentreUniversity of East AngliaNorwichUKNR4 7TJ Tel: +44(0)1603 591607Fax: +44(0)1603 591750E-mail: A.Goldson@uea.ac.uk