www.boldsystems.org

BARCODE OF LIFE DATA SYSTEMS (BOLD)

Riadul MannanBiodiversity Institute of Ontario

www.BOLDSYSTEMS.org

1 Architect, 2 Hardware Engineers

4 Programmers, 4 Data Managers

BOLD SYSTEMS450 CPUs, 100TB storage

Generator powerRemote backup

Barcode Records for Fungi , Protists, Plants and Animals 834.5Kspecimens, 69.6K species, 2.1K institutions : 5.1K users

Features of BOLD

Quality AssessmentsPrimer Registry

Alignment Viewer/EditorIntegrated Help

Taxon ID TreeDistance Summary

Sequence CompositionNearest Neighbour Summary

Accumulation CurvesMulti-Marker ID Engine

Bibliography SupportWeb Service API

Workflow *Analytics Integration

Specimen

Tissue Sample

ExtractDNA

PCR Amplify Sequence

Photograph

Collection Data

Web-Accessible Data andDNA Barcode

The Analytical Chain

Specimen

Taxonomy

Identifier

IDs

Institution

Collectors

Desc.

Lat/Lon

Country

Collection

Lab Data

Trace

Primers

Audit Trail

Marker 1

DNAMarker 2

Markers 3Caption

Metadata

Copyright

Image/Video

Literature

BOLD Data Model

BOLD Multi-Marker Data

Tag Cloud of markers

Total Markers on BOLD : 45

Project Management

Data ManagementSpecimen Sequence

Data Management

1 2

3

4

Primer Registry

Total Primer – 633Public – 325Private – 308

Data Analysis & Visualization

Sequence Analysis - Taxon ID Tree

Alignment Browser

Features:Flexible labeling

Variability of positionsMapping amino acids

Future Features:Highlighting diagnostic positionMapping to secondary structuresWeb based sequence editing

Identification System

Managing Lab Data - LIMS

LOCATING SAMPLES

OwnerSample TypeSampleidsProcessidsKeywordsMicroplate Label

Sample Lookup

Controlling Liquid Handling Robots

PCR Success Report

Gel image

Sliced by system

Management And Monitoring

LIMS - Non Lab User

Data Publication