WORLD PRECISION INSTRUMENTS
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WORLD PRECISION INSTRUMENTS MAKE YOUR ZEBRAFISH RESEARCH EASY & COST-EFFECTIVE WITH WPI Using oocytes, embryos, larvae and adults www.wpiinc.com Zebrafish microdissection instruments ZEBRAFISH SURGICAL INSTRUMENT KIT I WPZ00018 Kit Includes: • Probe, 0.25 mm Tips, 45° angle, 15 cm (WPI0218) • Probe, 0.25 mm Tips, 15 cm (WPI0118) • Student Vannas Scissors, 9 cm, Straight, 500 µm Tip (501777) • Microdissecting Forceps, 10.2 cm, Angled, Serrated (504479) ZEBRAFISH SURGICAL INSTRUMENT KIT 2 WPZ00218 Kit Includes: • Probe, 0.25 mm tips, 15 cm (WPI0118) • Probe, 0.25 mm Tips, 45° angle, 15 cm (WPI0218) • Noyes Scissors, 12 cm, Straight, Sharp/Sharp, 15 mm Blades (500228) • Tissue Forceps,1x2 Teeth (15918) • Iris Forceps, 10 cm, Curved, Serrated (15915) 15915 WORLD PRECISION INSTRUMENTS USA: 175 Sarasota Center Boulevard, Sarasota FL 34240-9258 USA Tel: (941) 371-1003 • Fax: (941) 377-5428 • [email protected] • www.wpiinc.com UK: 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ England • 44 (0)1462 424700 • [email protected] Germany: Pfingstweide 16, D-61169 Friedberg (Hesson) • Tel: +49 (0)6031 1602171 • Fax: +49 (0)6031 1602180 • [email protected] China and Hong Kong: Rm 18A, No8 Donfang Rd., Pudong District, Shanghai 200120 PRC • +86 688 85517 • [email protected] Brazil: Conselheiro Nabias, 756 sala2611, Santos-São Paulo 11045-002 Brazil • [email protected] References 1 Eric S. Clelland, Qian Tan, Ari Balofsky, Rosie Lacivita, Chun Peng. Inhibition of Premature Oocyte Maturation: A Role for Bone Morphogenetic Protein 15 in Zebrafish Ovarian Follicles. Endocrinology, Volume 148, Issue 11, 1 November 2007, Pages 5451–5458,https://doi.org/10.1210/en.2007-0674 2 Rosen JN, Sweeney MF, Mably JD. Microinjection of zebrafish embryos to analyze gene function. J Vis Exp. 2009 Mar 9;(25). pii: 1115. doi: 10.3791/1115. 3 Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., Hukriede, N. A. Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury. J. Vis. Exp. (42), e2079, doi:10.3791/2079 (2010). 4 Kinkel, M. D., Eames, S. C., Philipson, L. H., Prince, V. E. Intraperitoneal Injection into Adult Zebrafish. J. Vis. Exp. (42), e2126, doi:10.3791/2126 (2010). 5 Staudt, D. & Stainier, D. Uncovering the molecular and cellular mechanisms of heart development using the zebrafish. Annu Rev Genet 46, 397–418 (2012). 6 Hu N1, Yost HJ, Clark EB. Cardiac morphology and blood pressure in the adult zebrafish. Anat Rec. 2001 Sep 1;264(1):1-12. 7 Katerji M1, Filippova M1, Duerksen-Hughes P1. Approaches and Methods to Measure Oxidative Stress in Clinical Samples: Research Applications in the Cancer Field. Oxid Med Cell Longev. 2019 Mar 12;2019:1279250. doi: 10.1155/2019/1279250. eCollection 2019. 8 Alugoju Phaniendra, Dinesh Babu Jestadi, and Latha Periyasamy. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015 Jan; 30(1): 11–26. 501777 WPI0118 WPI0118 504479 15918 500228 WPI0218 WPI0218 U.S. PATENT 5,294,325 OTHER ACCESSORIES The WPI M3301 Micromanipulators, Electrode Holders, Pre-Pulled Glass Micropipttes and Z-MOLDS were used in this application. The CardioPhys™ Z ECG is not shown. Popular MiniStar peristaltic pump is perfect for small applications. WPI’s MicroFil™ fills micropipettes easily and reliably by starting close to the pipette tip, eliminating air bubble forma tion. WPI offers biosensing macrosensors (2 mm) like this one and a range of microsensors for NO, O 2 , H 2 O 2 and H 2 S. Z-MOLDS Microinjection & Transplantation Molds (4 per kit) are turned up-side down and placed in liquid agarose gel. Pipette the embryos into the grooves. The embryos self- align.
Transcript of WORLD PRECISION INSTRUMENTS
WORLD PRECISION INSTRUMENTS
MAKE YOUR ZEBRAFISH RESEARCH EASY & COST-EFFECTIVE WITH WPI
Using oocytes, embryos, larvae and adults
www.wpiinc.com
Zebrafish microdissection instrumentsZEBRAFISH SURGICAL INSTRUMENT KIT IWPZ00018 Kit Includes: • Probe, 0.25 mm Tips, 45° angle, 15 cm (WPI0218)
• Probe, 0.25 mm Tips, 15 cm (WPI0118)
• Student Vannas Scissors, 9 cm, Straight, 500 µm Tip (501777)
• Microdissecting Forceps, 10.2 cm, Angled, Serrated (504479)
ZEBRAFISH SURGICAL INSTRUMENT KIT 2 WPZ00218 Kit Includes: • Probe, 0.25 mm tips, 15 cm
(WPI0118) • Probe, 0.25 mm Tips, 45° angle, 15 cm (WPI0218)
• Noyes Scissors, 12 cm, Straight, Sharp/Sharp, 15 mm Blades (500228)
• Tissue Forceps,1x2 Teeth (15918) • Iris Forceps, 10 cm, Curved, Serrated (15915)
15915
WORLD PRECISION INSTRUMENTSUSA: 175 Sarasota Center Boulevard, Sarasota FL 34240-9258 USATel: (941) 371-1003 • Fax: (941) 377-5428 • [email protected] • www.wpiinc.com
UK: 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ England • 44 (0)1462 424700 • [email protected] Germany: Pfingstweide 16, D-61169 Friedberg (Hesson) • Tel: +49 (0)6031 1602171 • Fax: +49 (0)6031 1602180 • [email protected] China and Hong Kong: Rm 18A, No8 Donfang Rd., Pudong District, Shanghai 200120 PRC • +86 688 85517 • [email protected] Brazil: Conselheiro Nabias, 756 sala2611, Santos-São Paulo 11045-002 Brazil • [email protected]
WORLD PRECISION INSTRUMENTS
References1 Eric S. Clelland, Qian Tan, Ari Balofsky, Rosie Lacivita, Chun Peng. Inhibition of
Premature Oocyte Maturation: A Role for Bone Morphogenetic Protein 15 in Zebrafish Ovarian Follicles. Endocrinology, Volume 148, Issue 11, 1 November 2007, Pages 5451–5458,https://doi.org/10.1210/en.2007-0674
2 Rosen JN, Sweeney MF, Mably JD. Microinjection of zebrafish embryos to analyze gene function. J Vis Exp. 2009 Mar 9;(25). pii: 1115. doi: 10.3791/1115.
3 Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., Hukriede, N. A. Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury. J. Vis. Exp. (42), e2079, doi:10.3791/2079 (2010).
4 Kinkel, M. D., Eames, S. C., Philipson, L. H., Prince, V. E. Intraperitoneal Injection into Adult Zebrafish. J. Vis. Exp. (42), e2126, doi:10.3791/2126 (2010).
5 Staudt, D. & Stainier, D. Uncovering the molecular and cellular mechanisms of heart development using the zebrafish. Annu Rev Genet 46, 397–418 (2012).
6 Hu N1, Yost HJ, Clark EB. Cardiac morphology and blood pressure in the adult zebrafish. Anat Rec. 2001 Sep 1;264(1):1-12.
7 Katerji M1, Filippova M1, Duerksen-Hughes P1. Approaches and Methods to Measure Oxidative Stress in Clinical Samples: Research Applications in the Cancer Field. Oxid Med Cell Longev. 2019 Mar 12;2019:1279250. doi: 10.1155/2019/1279250. eCollection 2019.
8 Alugoju Phaniendra, Dinesh Babu Jestadi, and Latha Periyasamy. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015 Jan; 30(1): 11–26.
501777
WPI0118
WPI0118
504479
15918
500228
WPI0218
WPI0218
U.S. PATENT 5,294,325
OTHER ACCESSORIES
The WPI M3301 Micromanipulators, Electrode Holders, Pre-Pulled Glass Micropipttes and Z-MOLDS were used in this application. The CardioPhys™ Z ECG is not shown.
Popular MiniStar peristaltic pump is perfect for small applications.
WPI’s MicroFil™ fills micropipettes easily and reli ably by starting close to the pi pette tip, elim i nat ing air bub ble for ma tion.
WPI offers biosensing macrosensors (2 mm) like this one and a range of microsensors for NO, O2, H2O2 and H2S.
Z-MOLDS Microinjection & Transplantation Molds (4 per kit) are turned up-side down and placed in liquid agarose gel. Pipette the embryos into the grooves. The embryos self-align.
SOLUTIONS FOR STUDIES WITH ZEBRAFISH AT DIFFERENT LIFE STAGES Oocytes, Embryo, Larvae and Adults
Direct cardiovascular measurements Embryos, larvae and adult fishEmbryonic zebrafish have become a popular model for cardiovascular research.5 WPI is a pioneer in developing instruments for research in electrophysiology and is cited in 1000s of peer-reviewed publications. WPI introduced the CardioPhys™ Z series of products that makes measurement of cardiovascular functionalities in zebrafish embryo easy.
WPI CARDIOPHYS™ Z ECGThe novel WPI CardioPhys™ Z ECG allows direct measurement of ECG in zebrafish embryos. WPI has optimized its existing electrophysiology product line to meet the demands of the zebrafish researcher. • Ensures very low noise for recording electrical activity of zebrafish embryonic heart
• High resistance to environmental electrical noise
• User-friendly software • Allows researchers to identify diagnostic indices of cardiovascular physiology such as altered rhythm, signal conduction abnormalities, damage from cardiac events such as ischemia and infarction, etc.
CARDIOPHYS™ Z BPThe new WPI CardioPhys™ Z BP is designed to record hydrostatic pressure in very small vessels using a Servo-Null system.6 The system has been used to measure blood pressure in the Zebrafish (embryo, larvae and adult fish) with less than 0.5 mmHg resolution.(Right) Pressure signal from the WPI CardioPhys™ Z BP from the ventricle (top) and bulbus arteriosis (bottom) in a larva.
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RESEARCH AREAS
Developmental Biology Oncology Toxicology
Genetics Regenerative Medicine Pharmacology
Detection of free radicals & other molecules for disease models
This is a typical laboratory setup of a WPI free radical analyzer with data acquisition system.
The WPI Free Radical Analyzer, TBR4100, enables real-time, highly sensitive detection of free radicals such as NO, H2O2, H2S, CO, O2–important physiological indicators in intracellular cell signaling and homeostasis. Free radicals beyond the level of normal detoxication can result in oxidative stress (OS).7 OS affects wound healing and aging, and it causes several diseases such as diabetes mellitus, neurodegenerative disorders (Parkinson’s disease, Alzheimer’s disease and Multiple sclerosis), cardiovascular diseases (atherosclerosis and hypertension), respiratory diseases (asthma), cataract development, rheumatoid arthritis and various cancers (colorectal, prostate, breast, lung, bladder cancers).8
The output shows the raw data for an ISO-NOPF200 (NO sensor). Only one of the four channels is used in this application.
Enhanced microinjection efficiency for genetic modification
The customizable Zebrafish Microinjection Toolbox includes components favored by researchers: SYS-PV820/SYS-PV830 Pneumatic PicoPump, PUL-1000 Micropipette Puller, PZMTIII-MI/PZMIII-MI Trinocular or Binocular Microscope on lighted base with articulating mirror, PRO-300HDS High Definition Camera and Monitoring System, MICRO-ePORE™ Cell Penetrator to facilitate microinjections, and accessories. The NANOLITER2010 Injector and the UMP3T-1 UltraMicroPump with SMARTouch Touchscreen Controller are alternate injectors.
WPI has been serving scientists for over 50 years with easy and cost-effective tools for genetic modification. WPI offers a wide range of instruments required for microinjection of zebrafish in oocytes,1 embryo,2 larvae3 and adults4 to enable genetic modification.
CardioPhys™ Z ECG graphical display
ECG monitoring using WPI CardioPhys™ Z ECG.
