Layar Merah Herbs

• Concern on the importance of skin tone• A fair and spotless skin, is

considered a sign of beauty and reveals certain social status.

• A skin with a dull tone, spots and irregular pigmentation looks much older than the real age

• Differences in skin color is due to melanin• Increase in melanin by external

aggression and aging leads to changes in skin tone

• Surgery is costly, risky and expensive

• Surgery is aggressive and invasive

• Cosmetics is Natural, Safe and Effective

• Whitening Cream decreases skin tone evenly and uniformly and reduces different types of spots

• Dual action• Prevents – Reduces melanin synthesis

• Treats – Evens out skin tone, reduces spots in number and intensity

• Reduces process of spontaneous melanin synthesis

• Prevents an accumulation of melanin in the skin by reducing melanocyte dendrites

• Reduces tyrosinase enzyme formation by 63%, therefore it reduces melanogenesis

• Decreases the amount of pigment synthesized by melanocytes by 34%, without affecting their viability

• Modulates melanocyte gene expression

• Resulting in whitening of skin

• Instant skin tone whitening effect

• Long-term brightening effect

• Instant radiance boosting and moisturizing

• Decreases skin tone evenly and uniformly and reduces different types of spots

• Lightening active ingredient in Whitening Cream diminishes melanogenic activity and decreases skin tone and spots

• Tissue enlightment

• Preventing or inhibiting skin pigmentation

• Whitening Cream has an intelligent depigmentant; its activity is higher in areas with spots and thus evens skin color

• Reduces spots in number and intensity, regardless of their origin

• Reduces both tyrosinaseenzyme formation and activity, as well as melanin synthesis, all without affecting melanocyte viability.

• Decreases skin tone in a uniform and homogenous way

• It reduces the number and intensity of different types of hyperpigmentation spots.

• Several test were performed to assess the in vitro efficacy

• PPARγ binding affinity

• Study on depigmentation activity in human melanocytes :

• Evaluation of tyrosinase expression

• Evaluation of tyrosinase activity

• Evaluation of melanin quantity

• Incubation of NHEM cells (Normal Human Epidermal Melanocytes) was conducted at a concentration of 0.7% for 6 and 9 days.

• The active ingredients of Whitening Cream shows a good affinity to bind to PPARγ and act as a natural agonist

• It reduces tyrosinase enzyme formation by 63%, therefore it reduces melanogenesis

• It reduces tyrosinase activity by 55%

• It decreases the amount of pigment synthesized by melanocytes by 34%, without affecting their viability

• It prevents an accumulation of melanin in the skin by reducing melanocyte dendrites

• In vitro evaluation of melanin diminution• Application of 4 mg/cm² of whitening creams on the surface

of reconstructed skin day 0, 1, 5, 6, 7, 8

• Spectrophotometric measurement of the rate of melanin compared to a control after 9 days

• In vitro evaluation of skin lightening : ∆E• Epidermis color measurement was performed before starting

treatment (Day 0) and 24h after the last application of Whitening Cream on tissues containing melanocytes and keratinocytes from African-american donors (spontaneusproduction of melanin)

• Application of 4 mg/cm² of whitening creams on the surface of reconstructed skin day 0, 1, 5, 6, 7, 8

• Chromatometric measure of the degree of pigmentation of tissues. Quantification of the color of tissues using a Chroma Minolta CR-400. Recording parameters L * (lightness), a * and b * (chromaticity coordinates) � Colour difference ∆E

• In vitro evaluation of skin lightening : ∆ ITA°

• Same than for ΔE with a skin spontaneus production of melanin using a Chroma Minolta CR-400 and calculation of Δ

ITA°. ITA angle defines the degree of skin pigmentation �darker skin have lower ITA° values

• In vitro evaluation of melanin diminution shows it has a significative whitening activity of around 25%. Melanin diminution is linked to the inhibilition of tyrasinase

• In vitro evaluation of skin lightening : ∆E, shows a higher ∆E shows a higher darkening ; it has a significant whitening activity

• After 9 days of treatment the ITA°values for tissues treated are higher than untreated tissues indicating a lighter tissue coloration compared to untreated tissues. It reduce the process of spontaneous melanin synthesis during the test

• In vivo efficacy

• 20 Asian volunteers

• 3% cosmetic formulation with active ingredient on one side of the face, and placebo on the other.

• 56 days of twice daily application.

• Two areas of the same volunteer: Normal skin and spotted skin

• Normal skin :• It decreases the intensity of skin

pigmentation• The ITA° parameter increases up to

28% in skin without spots, with a mean variation of 3%

• Skin with spots :• It lightens hyperpigmented areas

getting a smooth and uniform complexion

• Whitening Cream has an intelligent depigmentant; its activity is higher in areas with spots and thus evens skin color

• It reduces spots in number and intensity, regardless of their origin

• The depigmenting potential of the active ingredient of the Whitening Cream was assessed using human tanned in vitro reconstructed epidermis. This in vitro experimental study was based on the measurement of melanin content and on the coloration of tissues after 9 days topical treatment. The results show that it imparts :

• Significant melanin reduction

• Tissue enlightment

• This corroborates the down-regulating effect on melanin level. These good results of melanin contents diminution and lighter coloration after treatment prove the whitening and depigmenting properties of the active ingredient

• Objectivation : Illuminating

• It contains mica to boost radiance of the skin for a radian, healthy looking appearance

• Objectivation : Moisturizing

• It has moisturizing properties thanks to the polymeric coating

• Cosmetic benefits

• Instant whitening effect with a natural complexion – Lighten skin tone or provide an even skin complexion

• Instant illuminating effect and moisturizing – A radiance-boosting complexion perfector that smoothes and evens the skin for a luminous glow and radiant, healthy-looking appearance

• Long-term whitening effect with a surface treatment based on an efficient whitening active – Preventing or inhibiting skin pigmentation

• Skin whitening facial treatments

• Specific anti-spot facial treatments

• Skin lightening body lotions

• Anti-spot hand treatments

• Throat and neckline treatments

• Photo-aging treatments

• Anti-age treatments

• Discontinue use of other products 3 days prior to beginning use of Whitening Cream

• Cleanse skin thoroughly• Apply Whitening Cream to skin twice daily, gently

massage until all traces of cream disappear into the skin• For best results, apply twice daily until desired results are

achieved• Apply in the morning• Allergic reactions are NOT COMMON and only happen to

a select group of people with very sensitive skin • People with sensitive skin may react to the preservative

ingredients in the cream

• You should take a SKIN ALLERGY TEST with the cream before applying these products to your face and skin

• The best way to prevent an allergic reaction is to know if one will occur

• Prior to using the cream, apply a thin layer to a tiny spot on the face that can be covered by your hair

• Throughout the day, observe it to see if the spot becomes puffy, itchy or warm

• If all three occur, then you have an allergic reaction to the cream and should not use it at all

• Titanium dioxide

• Aluminum hydroxide

• Glycyrrhiza glabra (Licorice) Root Extract

• Alpha-arbutin

• Boerhavia diffusa Root Extract