What’s magnified?

What’s magnified?

What’s magnified?

Microscopy

Microscopes

• “micro” means small!• “scope” means watch/look!

Microscopes were first developed as

entertainment for the rich.

Light Microscopes

• Focuses visible light rays

• Can be used on live or dead specimens

• Dyes & fluorescent markers help for viewing

• Magnify up to 1000X on the ones we have!

• Compound Light Microscopes

Light Micrographs

Plant

Human Chromosomes

The Compound Light Microscope

The word compound means to increase! A compound microscope has 2 lenses to increase magnification!

Parts of the Microscope

1. Ocular Lens: eyepiece (10x magnification), first lens

2. Arm: connects base & body tube– Must hold arm while

carrying microscope!

3. Course Adjustment Knob: roughly focuses object– Used to find specimen

Parts of the Microscope

4. Fine Adjustment Knob: small knob, finely focuses specimen– Use after object has been

found!

5. Power Cord: must be wrapped around arm after use

6. Nosepiece: holds 3 objective lenses

Parts of the Microscope

7. Objective Lens: second lens– Scanning Lens (4x)– Low Power (10x)– High Power (40x)– Oil Immersion (100x)

Parts of the Microscope

8. Stage Clips: holds slide in place

9. Stage: flat plate where slides are held

10. Diaphragm: regulates the amount of light exposed to the specimen

Parts of the Microscope

11. Condenser Lens: focuses light on specimen

12. Power Switch: turns light on & off

13. Base: support of the microscope

Field of View

• Total area visible through the ocular lens

The higher the magnification, the smaller the field of view.

Total Magnification

Total Magnification = ocular lens x objective lens

If the 40x objective is in place…TM = 400x, because (10 x 40 = 400)

Focusing the Microscope

• Locate the slide specimen at scanning power.

• Then move the area to be viewed to the center of the field of view.

• Make sure the slide is in perfect focus at scanning power before moving up to low power.

• Turn your objective without moving the slide or stage.

• You will slightly adjust the image at the next objective.

• At high power use only the fine focus knob and do not turn it more than 1/2 turn.

Important! Microscope Rules

• Always carry with 1 hand on arm & 1 hand on base!• Use a slide & coverslip.

• Clean Up:– Always return to scanning (lowest) power!– Always lower stage all the way!– Unplug, wrap cord, & cover

Intro. Microscope Lab

NO HORSEPLAY!!

• Follow the directions carefully… don’t skip over things just because you don’t feel like reading!