What is Semi-Log Graph paper…. And WHY do I need to know?
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Transcript of What is Semi-Log Graph paper…. And WHY do I need to know?
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What is Semi-Log Graph paper…. And WHY do I need
to know?
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DNA can be cut, using RESTRICTION ENZYMES… into
segments of differing lengths.
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After DNA is separated on an agarose gel by electrophoresis,
the migration of fragments of known size can be compared to
that of fragments of unknown size.
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• Suppose we have digested DNA from two viruses ~ both with Hind III,
• And electrophoresed the DNA from both on an agarose gel
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• Electrophoresing the DNA on an agarose gel ~~ causes the migration of the fragments.
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• After running the gel ~ you measure the distance the DNA migrated.
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Table 1. Restriction enzyme analysis of λ and UPCFV DNA
λ Virus U.P. Cabin Fever Virus (UPCFV)
DNA Hind III digest DNA Hind III digest
Size (kb) Distance (mm) Size Distance (mm)migrated migrated
23.1 3 ? 5 9.42 14 ? 12 6.56 18.5 ? 15 4.36 23.5 ? 27 2.32 32. 2.03 34 0.56 50
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In order to determine the sizes of the U.P. Cabin Fever Virus DNA, you will make a standard
curve for the known Lamda DNA
• YUK! This does not show a linear relationship between the fragment size and distance migrated… down towards the right side of the graph, it would be difficult to determine size…
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Size of fragment
Leng
th m
igra
ted
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• A logarithmic graph, basically takes an EXPONENTIAL graph and changes it to a LINEAR one … but using funky graph paper & by using the log function. (log function is the inverse of an exponential function)
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Size of fragment
Leng
th m
igra
ted
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• When a curve is not straight.. It can be made straight with logarithms.
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migration
Log
of f
ragm
ent
leng
th
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Scientists use logarithmic graph paper to manage
large amounts of data easily
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• And when you are talking kilo base pairs…. And DNA….
You are discussing a
LARGE amount of data
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Qu
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See the funky graph paper?The axis with
the logarithmic info runs in
cycles…
The cycles differ by a factor of 10
btw, this is an example of 6 cycle graph paper
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Qu
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See the funky graph paper?
One of the virtues of
semi-logarithmi
c plots is their
ability to show a
very wide range of
values.