ZymoPURE™

What if Plasmid Purification was this simple?

ZymoPURE™: One Technology for all of your Plasmid NeedsEmpower your research with ZymoPURE™ plasmid DNA purification kits. Streamlined methodology and superior technology enables unrivaled speed and performance. Innovative binding technology enables DNA to be purified using a microcentrifuge column via vacuum or centrifuge in as little as 15 minutes. Ultra-pure, transfection-grade plasmid DNA is eluted directly from the spin-column into a 1.5 ml microcentrifuge tube and is ready for sensitive downstream applications.

Innovation. Pure & Simple.™

Innovative, PURE™ technology matrix

unique column design enables elution from a microcentrifuge (Mini, Midi, Maxi)

unique 45° angle prevents retention of contaminants

Luer Lock cap facilitates vacuum or centrifuge-based processing

Featuring the ZymoPURE™ Midiprep Spin-Column

• 25x higher binding capacity• orders of magnitude better

endotoxin removal• the lowest elution volumes

From E. coli

Miniprep Scale

ZymoPURE™ Plasmid Miniprep Kit

Rapid purification of ≤ 100 μg transfection-grade plasmid

DNA

Midiprep, Maxiprep, and Gigaprep Scales

ZymoPURE™ Kits ZymoPURE-EndoZero™ ZymoPURE-Express™

Transfection-ready plasmid DNA in ≤18 minutes, using

a spin-column: Midi ≤ 300 μg

Maxi ≤ 1200 μgGiga ≤ 10 mg

Ultra-low endotoxin levels (≤ 0.025EU/ug) in under

20 minutes

Pellet free isolation. Culture to transfection

in only 15 minutes. No large-scale centrifugation

ZymoPURE™ Plasmid Miniprep KitHighlights

• Fast and reliable purification of up to 100 µg of transfection-grade plasmidDNA using a spin-column.

• Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.

Superior YieldsTransfection-grade

Supplier Q Supplier MNM ZymoPURE™

than the industry leading miniprep kit

50,000 timesfewer ENDOTOXINS

Product Cat. No. Size

ZymoPURE™ Plasmid Miniprep Kit

D4208T 10 preps.

D4209 50 preps.

D4210 100 preps.

D4211 400 preps.

D4212 800 preps.

Streamlined, Innovative Workflow

rapid loading onto a spin-column via vacuum or centrifuge

bindfor ultra-pure endotoxin free plasmid DNA

washtransfection ready plasmid DNA

elute

Plasmid DNA yield and concentration from the ZymoPure™ Miniprep Kit compared to other major suppliers. Plasmid DNA (pGL3®) was isolated from 5 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate).

ZymoPURE™ Plasmid Midiprep, Maxiprep, Gigaprep KitsHighlights

• Fast, easy, reliable, ultra-pure transfection-ready plasmid DNA directly froma spin column.

• Innovative ZymoPURE™ binding technology enables elution of the highestconcentration of plasmid DNA directly from a spin-column using amicrocentrifuge.

Highest Recovery100 times less endotoxins using ZymoPURE™

ZymoPURE™

Maxiprepavg: 0.0197 EU/µg DNA

Supplier Q Maxiprep avg: 2.5435 EU/µg DNA

Endotoxin Units/µg DNA

0 1 2.50.5 21.5 3

Product Cat. No. Size

ZymoPURE™ Plasmid Midiprep KitD4200 25 preps.

D4201 50 preps.

ZymoPURE™ Plasmid Maxiprep KitD4202 10 preps.

D4203 20 preps.

ZymoPURE™ Plasmid Gigaprep Kit D4204 5 preps.

Supplier Q1 Supplier Q2M

ZymoPURE™

Plasmid DNA yield and concentration from the ZymoPURE™ Maxiprep Kit compared to other major suppliers. PlasmidDNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Endotoxin levels of plasmid DNA isolated using the ZymoPURE™ Maxiprep kit compared to maxiprep kits from Supplier Q. Plasmid (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protcol (in duplicate).

Streamlined, Innovative Workflow

rapid loading onto a spin-column via vacuum or centrifuge

bindfor ultra-pure endotoxin free plasmid DNA

washtransfection ready plasmid DNA

elute

ZymoPURE-EndoZero™ Plasmid KitsHighlights

• Consistently achieve endotoxin levels of ≤ 0.025 EU/µg of plasmid DNA inonly 20 minutes.

• Plasmid DNA is ideal for transfection in sensitive cells and in vivo research.• The unique spin-column based procedure purifies up to 1.2 mg of endotoxin-

free plasmid DNA.

Streamlined, Innovative Workflow

Achieve the Lowest Endotoxin Levels for the Most Sensitive Applications

Featuring EndoZero™ Column Technology

Simply use the EndoZero™ spin-column to remove

additional endotoxins from the ZymoPURE™ eluate

Endotoxin removal in minutes!

ZymoPURE™ technology paired with Zymo’s proprietary EndoZero technology has set a new industry standard for endotoxin levels, with its ultra endotoxin-free EndoZero™ columns.

Supplier QEndoFree

ZymoPURE™ - EndoZero

Supplier Q

Endo

toxi

n U

nits

/µg

Plas

mid

DN

A

FDA Limit for Vaccines< 0.04 EU/µg DNA

0.000

0.025

0.050

0.075

0.100

2

4

6

8

10

Product Cat. No. Size

ZymoPURE-EndoZero™ Plasmid Maxiprep Kit D4205 10 preps.

ZymoPURE-EndoZero™ Plasmid Gigaprep Kit D4207 5 preps.

Endotoxin levels in plasmid DNA isolated using the ZymoPURE-EndoZero™ Maxiprep kit from Zymo Research. Plasmid DNA (pGL3®) was isolated from 150 ml ofJM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). Endotoxin Concentration was measured in the eluted plasmid DNA using the chromogenic Pyrochrome® endotoxin specific assay from Associates of Cape Cod Incorporated. Shown are means ± SD of 2 preps.

bindrapid loading onto a spin-column via vacuum or centrifuge

washfor ultra-pure endotoxin-free plasmid DNA

transfection ready plasmid DNA

elutefor removal of additional endotoxin via centrifuge

filter

Highlights

• Innovative pellet-free procedure bypasses the standard cell-pelleting andresuspension steps. No centrifugation required!

• The fastest, easiest, most reliable method for purification of up to 1.2 mg ofultra-pure endotoxin-free plasmid DNA.

• Plasmid DNA is eluted directly from a microcentrifuge column and is well-suited for transfection and other sensitive applications.

ZymoPURE-Express™ Plasmid Midiprep Kit

Streamlined, Innovative WorkflowNo large-scale centrifugation

Superior Yield The Fastest Method for Plasmid Purification

neutralizeusing Zymo’s patented color buffer system

rapid loading onto a spin-column via vacuum or centrifuge

bind/washtransfection ready plasmid DNA

elutelysedirectly from culture without cell pelleting

Plasmid DNA yield and concentration from the ZymoPURE Express™ Midiprep Kit compared to other major suppliers. Plasmid DNA (pGL3) was isolated from 25 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol in duplicate. The eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Save up to 100 minutes with the ZymoPURE™-Express Midiprep Kit.

Plasmid DNA yield and concentration from the ZymoPURE – Express™ Maxiprep kit compared to other major suppliers. Plasmid DNA (pGL3) was isolated from 25 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol in duplicate. The eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

MZymoPURE™ Supplier Q1 Supplier Q2 Supplier S Supplier P Supplier MN

Product Cat. No. Size

ZymoPURE-Express™ Plasmid Midiprep Kit D4213 25 preps.

Zymo Research

Supplier Q

Supplier MN

Supplier P

Supplier I

Supplier T

Supplier S

0

20

40

60

80

100

120

Proc

essi

ng T

ime

(min

)

Only 15 Minutes!

*Ampicillin selection only

Related Products

Zyppy® Plasmid Kits

Mix & Go Competent Cells

Zymoclean™ Gel DNA Recovery Kits

Product Cat. No. Size

Zymoclean™ Gel DNA Recovery Kit (uncapped)

D4001 50 preps

D4002 200 preps

Zymoclean™ Gel DNA Recovery Kit (capped)

D4007 50 preps

D4008 200 preps

ZR-96 Zymoclean™ Gel DNA Recovery KitD4021 2 x 96 preps

D4022 4 x 96 preps

Zymoclean™ Large Fragment DNA Recovery Kit

D4045 25 preps

D4046 100 preps

DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.

M 1 2 3 4 5

23 kb -9 kb -

2 kb -

500 bp -

Boost recoveries from agarose gels to > 80%

Pellet-free plasmid preparations

Mix & GoCompetent Cells

Others

Place on Ice

Spin to Concentrate Cells

Remove SupernatantAdd SOC

20 second* transformations!

Ice 45 min.

EcoRI digestion of plasmid DNA (pGEM®) isolated from E. coli culture using the Zyppy™ Plasmid Miniprep Kit or the QIAprep™ Spin Miniprep kit from Qiagen. The amount of DNA loaded was standardized based on culture volume input. Performed in triplicate.

1 kb Zymo Research Supplier Q Product Size Cat. No.

Zyppy™ Plasmid Miniprep Kit50 Preps. D4036

100 Preps. D4019

Zyppy™-96 Plasmid Miniprep Kit2 x 96 Preps. D4041

4 x 96 Preps. D4042

Zyppy™-96 Plasmid MagBead Miniprep2 x 96 Preps. D4100

4 x 96 Preps. D4101

Zyppy™ Plasmid Midiprep Kit25 Preps. D4025

50 Preps. D4026

Strain 10 x 100 µl 95 x 50 µl

Zymo 5α(DH5α) T3007 T3009

JM109 T3003 T3005

Zymo 10B(DH10B) T3019 T3020

TG1 T3017 -

42°C 2 min. (Heatshock)

Make Your Own Size Cat. No.

Mix & Go ! E. coli Transformation Kit 20 ml T3001

Mix & Go ! E. coli Transformation Buffer Set 60 ml T3002

Service Technique (réactifs et instrumentation) : 01 34 60 60 24 - tech@ozyme.fr Service Commande - Clients : 01 34 60 15 16 – commande@ozyme.fr

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