What are the differences in gut physiology between formula and breastfed infants? Does the addition...
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Transcript of What are the differences in gut physiology between formula and breastfed infants? Does the addition...
What are the differences in gut physiology between formula and breastfed infants? Does the addition of
prebiotics and probiotics in formula mimic the effects of breast milk?
Sahira Copenhaver
Nutrition 490W
Dr. Patterson
Spring 2011
April 22, 2011
Outline• Definitions• Intestinal microflora of breastfed vs. formula fed infants• Studies evaluating formulas with prebiotics and probiotics– Pre-term
• Westerbeek et al (2010)
– Term• Rinne et al (2005)• Bakker-Zierikzee et al (2005)
• Where is research heading?• Dietitian’s role• Conclusions
Definitions
• Human Milk Oligosaccharides (HMOs)• Functional Food Components– Probiotics• Bifidobacteria and lactobacilli
– Prebiotics • Galacto-oligosaccharides (GOS)• Fructo-oligosaccharides (FOS)
Thomas et al Pediatrics 2010;126:1217-1231.
1
Intestinal Microflora of Breastfed vs. Formula Fed Infants
• Breastfed– Dominated by populations of Bifidobacteria
• Formula fed–More complex microflora with Bifidobacteria,
facultative anaerobes, bacteroides, and clostridia
Mountzouris et al British Journal of Nutrition 2002;87:405-420.
2
Enteral Supplementation of Prebiotic Mixture on Intestinal Permeability in Preterm Infants
• Subjects– 113 infants, gestational age <32 weeks and/or birth
weight <1500g
• Methods– Randomly allocated • Prebiotic mixture of 80% GOS/FOS + 20% AOS• Placebo mixture (maltodextrin)
– Intestinal Permeability
Westerbeek et al British Journal of Nutrition 2011;105:268-274. 3
4
0 1 2 Time point
Westerbeek et al British Journal of Nutrition 2011;105:268-274.
L/M
R
atio
0.5
0.4
0.3
0.2
0.1
0.0
No Effect on L/M Ratio in Both Groups
Enteral Supplementation of Prebiotic Mixture not Correlated with Intestinal Permeability in Preterm Infants
• Results– Baseline patient and nutritional characteristics
were not different between groups– LBW increased the L/M ratio
• Limitations– Timing
Westerbeek et al British Journal of Nutrition 2011;105:268-274.
5
Similar Bifidogenic Effects on Infant Gut Microflora
• Subjects– 32 infants, born between 36 and 42 weeks of
gestation
• Study Design (Four Groups)– Formula (n=8)*– Pre + Formula – Breast milk – Pro + Breast milk
Rinne et al FEMS 2005;43:59-65. 6
Similar Bifidogenic Effects on Infant Gut Microflora
• Methods– Fluorescent in situ hybridization (FISH)– Bifidobacterium species characterization
• Results– Bifidobacteria lower among formula group
(P=0.044)– Bifidobacterium composition achieved in infants
receiving prebiotic supplemented formula
Rinne et al FEMS 2005;43:59-65.
7
Bifidobacterium Composition Achieved
8Rinne et al FEMS 2005;43:59-65.
Infant Formula Containing a Mixture of GOS/FOS or Bb-12 Compared to Standard
• Subjects– 63 pregnant women breastfeeding, 57 not
breastfeeding
• Feeding Groups–Mothers who decided not to breastfeed• Standard formula (n=19)• Prebiotic formula; GOS/FOS (n-19)• Probiotic formula; Bb-12 (n=19)
– BreastfeedingBakker-Zierikzee et al BJN 2005;94:783-
790.9
Infant Formula Containing a Mixture of GOS and FOS Results in Higher Bifidobacteria
• Methods– Questionnaires– FISH• Fecal samples
• Results
Bakker-Zierikzee et al BJN 2005;94:783-790.
10
GOS/FOS Formula has a Higher Percentage of Bifidobacteria in the Total Bacterial Count
11
Breast milkStandard formulaBb-12GOS/FOS
Where is Research Heading?
• Development of new prebiotic blend formula
• Microflora modulation can occur through diets– Is the effect long lasting?– Optimal composition and dosage
• Clarify specific effects: understand HMO’s benefit for the breastfed infant
12
Dietitian’s Role
• Encouragement of breastfeeding is the first choice
• Whenever breastfeeding is not possible– Supplemented infant formula provides safe, nutritious,
and healthy food for growth and development
• Keep abreast with the latest research
Huerou-Luron et al Nutrition Research Reviews 2010;23:23-36.
13
Conclusions• Enteral supplementation of a prebiotic mixture does not
enhance postnatal decrease in intestinal permeability in preterm infants in the first week of life
• Infants given partially hydrolyzed formula supplemented with prebiotics had identical levels of Bifidobacteria in feces, exceeding formula fed infants
• With the aim of stimulating the typical intestinal microflora of breastfed infants in formula fed infants, the effects of adding pre or probiotics to infant formula was compared to a standard formula. Although not statistically significant, GOS/FOS group had higher percentage of Bifidobacteria
14
The field is still in its infancy and it has to grow with care …
15
References1. Thomas DW, Greer FR, et al. Probiotics and prebiotics in pediatrics. Pediatrics
2010;126:1217-1231.
2. Mountzouris KC, McCartney AL, Gibson GR. Intestinal microflora of human infants and current trends for tis nutritional modulation. British Journal of Nutrition 2002;87:405-420.
3. Westerbeek EAM,van den Berg A, Lafeber HN, Fetter WPF, van Elburg RM. The effect of enteral supplementation of a prebiotic mixture of non-human milk galacto-, fructo- and acidic oligosaccharides on intestinal permeability in preterm infants. British Journal of Nutrition 2011;105:268-274.
4. Rinne MM, Gueimonde M, Kalliomaki M, Hoppu U, Salminen SJ, Isolauri E. Similar bifidogenic effects of prebiotic-supplemented partially hydrolyzed infant formula and breastfeeding on infant gut microbiota. FEMS 2005;43:59-65.
5. Bakker-Zierikzee AM, Alles MS, Knol J, et al. Effects of infant formula containing a mixture of galacto- and fructo- oligosaccharides or viable Bifidobacterium animalis on the intestinal microflora during the first 4 months of life. BJN 2005;94:783-790.
6. Huerou-Luron IL, Blat S, Boudry G. Breast v. formula-feeding: impacts on the digestive tract and immediate and long term health effects. Nutrition Research Reviews 2010;23:23-36.