WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and...
3
R atnervous cells cultured w ith gp41 and T. gondii N itric O xide Production D ays ofIncubation N itric O xide Production (uM ) 0 10 20 30 40 G roup A (C ontrol;n=15) G roup B (gp41,10 nM ;n=15) G roup C (T.gondii,1/500;n=15) G roup D (gp41/T.gondii;n=15) D ay 1 D ay 3 D ay 5 D ay 7 *** ***p<0.0001 *** *** *** *** *** *** *** *** *** *** *** *** *** *** *** WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and Toxoplasma gondii Escobar Guevara, EE; Fuentes Alvarado, YJ; Carrero Salas, JA; Roldán Dávila, YB; Alarcón de Noya, B; de Quesada, ME; Alfonzo Díaz, MA [email protected] ric Oxide Production in primary cultures of rat nervous cells, stimulated with gp41 and/or infected w of Sprague Dawley rats fetuses cultured under recombinant HIV’s gp41 stimulation (10 nM) with or wit ion (1/500; parasite/nervous cell ratio) (Groups B and D) produced significantly higher levels of nit o day 7, than groups without gp41 stimulation (Groups A and C). In our experiments, T. gondii infection neither inhibited the production of NO.
-
Upload
frederick-whitehead -
Category
Documents
-
view
215 -
download
3
Transcript of WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and...
Rat nervous cells cultured with gp41 and T. gondii
Nitric Oxide Production
Days of Incubation
Nit
ric
Ox
ide
Pro
du
cti
on
(u
M)
0
10
20
30
40
Group A (Control; n=15)
Group B (gp41, 10 nM; n=15)
Group C (T.gondii, 1/500; n=15)
Group D (gp41/T.gondii; n=15)
Day 1 Day 3 Day 5 Day 7
***
***p<0.0001
******
***
*** ******
****** ***
******
*** ******
***
WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and Toxoplasma gondiiEscobar Guevara, EE; Fuentes Alvarado, YJ; Carrero Salas, JA; Roldán Dávila, YB; Alarcón de Noya, B; de Quesada, ME; Alfonzo Díaz, MA
Fig. 1- Nitric Oxide Production in primary cultures of rat nervous cells, stimulated with gp41 and/or infected with T. gondii:Nervous cells of Sprague Dawley rats fetuses cultured under recombinant HIV’s gp41 stimulation (10 nM) with or without T. gondii-RH strain infection (1/500; parasite/nervous cell ratio) (Groups B and D) produced significantly higher levels of nitric oxide (NO) from day 1 to day 7, than groups without gp41 stimulation (Groups A and C). In our experiments, T. gondii infection nor induced
neither inhibited the production of NO.
Rat Nervous Cells Cultured with gp41 and T. gondii
Apoptosis
Days of Incubation
Ap
op
tosi
s (%
)(A
nn
exin
V)
0
2
4
6
8
10Group A (Control; n=12)
Group B (gp41, 10 nM; n=12)
Group C (T.gondii, 1/500; n=12)
Group D (gp41/T.gondii; n=12)
Day 1 Day 3 Day 5 Day 7
***
** **
***
*******
**
** p<0.01
*** p<0.0001
WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and Toxoplasma gondiiEscobar Guevara, EE; Fuentes Alvarado, YJ; Carrero Salas, JA; Roldán Dávila, YB; Alarcón de Noya, B; de Quesada, ME; Alfonzo Díaz, MA
Fig. 2- Apoptosis in primary cultures of rat nervous cells, stimulated with gp41 and/or infected with T. gondii:Nervous cells of Sprague Dawley rats fetuses cultured under recombinant HIV’s gp41 stimulation (10 nM) with or without T. gondii-RH strain infection (1/500; parasite/nervous cell ratio) (Groups B and D) showed higher levels of apoptosis from day 3 to day 7 (significantly days 5 and 7), than groups without gp41 stimulation (Groups A and C). When we compared nitric oxide production
(see Fig.1) with the percentage of apoptosis of rat nervous cells, from day 1 to day 7, in all culture conditions, we found a significantly positive correlation between these two variables (See Fig. 2-Insert). In our experiments, T. gondii infection nor induced neither
inhibited the apoptosis of rat nervous cells.
Rat Nervous Cells cultured with gp41 and T. gondiiApoptosis Vs. Nitric Oxide
(Días 1-7)
Nitric Oxide Production (uM)
0 10 20 30 40 50
Ap
op
tosi
s (%
)
0
5
10
15
20
25
Correl. Coef. = 0.575*** p<0.0001n=144
WEPDA0105: Destruction of nervous cells is potentiated in the simultaneous presence of gp41 and Toxoplasma gondiiEscobar Guevara, EE; Fuentes Alvarado, YJ; Carrero Salas, JA; Roldán Dávila, YB; Alarcón de Noya, B; de Quesada, ME; Alfonzo Díaz, MA
Rat Nervous Cells Cultured with gp41 and T. gondii
Cell Counts
Days of Incubation
Cel
l Co
un
ts (T
ota
l Nu
mb
er o
f C
ells
)
0
100x103
200x103
300x103
400x103
500x103
600x103
Group A (Control; n=22)
Group B (gp41, 10 nM; n=16)
Group C (T.gondii, 1/500; n=15)
Group D (gp41/T.gondii; n=9)
Day 1 Day 3 Day 5 Day 7
*p<0.05
** p<0.01
*** p<0.0001
***
***
*
******
*
Fig. 3- Cell counts of primary cultures of rat nervous cells, stimulated with gp41 and/or infected with T. gondii:Nervous cells of Sprague Dawley rats fetuses infected with RH strain of T. gondii (1/500; parasite/nervous cell ratio), with or
without recombinant HIV’s gp41 stimulation (10 nM) (Groups C and D), presented lower cell counts (significantly from day 3 to Group D and from day 5 to Group C) than Control (Group A), but in the simultaneous presence of gp41 and T. gondii (Group D)cell counts were even lower (significantly days 5, vs. Group C; and day 7, vs. Group B) suggesting potentiation of cytotoxicity.
In our experiments nor the presence of gp41 in cultures neither nitric oxide produced by its stimulation, inhibited the progression or cytolytic effect of T. gondii infection.
200x103
300x103
400x103
500x103
600x103
Group A (Control; n=22)
Group B (gp41,10 nM; n=16)
Group C (T.gondii, 1/500; n=15)
Group D (gp41/T.gondii; n=9)
Days of incubation
Day 1 Day 3 Day 5 Day 7
Rat Nervous Cells Cultured with gp41 and T. gondii
Cell Counts
Cel
l Co
un
ts (
To
tal
Nu
mb
er
of
Cel
ls)
*
** **
*
**
Vs. day 1:* p<0.05** p<0.01
+ : Vs. day 3# : Vs. day 5
+##
