Welcome to

2011 Thermo Scientific

Summer Symposium

Steve Vaughan

Tuesday 7th June

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Presentation Contents

• What is the Summer Symposium?

• Agenda for today

• Introducing Dionex

• New Product Introductions at ASMS this week

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Housekeeping Issues…

• Fire Exits

• Time Keeping; Agenda is tight for time, so please make

sure you are at your breakout session in plenty of time!

• Feedback; please tell us what you liked, or didn‟t, about

today‟s event!

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Thermo Scientific Summer Symposium- Purpose and Concept

An opportunity to learn about new developments in a number of technology and

application areas, and to share experiences and knowledge

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Feedback on last year seminars….

“For the last few years, Thermo Fisher

has promoted itself as a „one-stop

shop‟ but this here, today, is the first

time I‟ve seen that as a reality”

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Exhibition

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Registration and Breakout Rooms

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Agenda

09:00 - 09:30 Arrival & Registration

09:30 - 10:00

Welcome to the Day and NEW PRODUCT INTRODUCTIONS

Steve Vaughan

General Manager, Thermo Fisher Scientific

10:00 - 10:30

Helping Our Customers Make the World a Healthier, Cleaner and Safer Place!

Barry Adamson

Vice President, Global Accounts and UK Commercial Leader, Thermo Fisher Scientific

10:30 - 11:00 Coffee Break & Exhibition

11:00 - 16:30 Cell Culture & Biobanking

Clinical & Toxicology

Environmental

& Food Safety

Life Sciences &

Biotechnology

Materials Characterisation

& Testing

Keats (4th floor) Shelley (4th Floor) Wesley (4th floor) HERE! Wordsworth (4th floor)

12:15 - 13:30 Lunch & Exhibition

14:45 - 15:15 Tea Break & Exhibition

16:30 - 17:30 Wine Reception

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Introducing Dionex

Dionex products are now a part of the Thermo

Scientific brand, bringing leading capabilities in ion

chromatography, liquid chromatography, and

sample preparation to our portfolio, and creating

exciting new possibilities for scientific analysis.

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Dionex: Leading Chromatography Provider

Ion Chromatography Systems

• Leading technology

and market presence,

especially in

environmental and

food safety

Liquid Chromatography Systems

• UHPLC-ready across

entire platform,

comprehensive columns

offering

Chromatography Data Systems

• Gold standard

performance and

complementary to

our leading LIMS

Complements Our Leading Mass Spectrometry Offering

• Deep applications

expertise for compelling,

high productivity,

integrated solutions

Leading technology and performance across entire portfolio

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North America

29%

Europe 36%

Asia/Pacific 35%

A leading manufacturer and

marketer of chromatography

instruments, software,

consumables and related services

Life Science & Drug Discovery

Environmental Food & Beverage

Key End Markets

• Fiscal year 2010 Revenue:

$420 million

• 1,600+ employees

Product PortfolioGeographic Presence

IC

62%

ASE

7%

HPLC

31%

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Creating a Chromatography Powerhouse

Instruments$325M

Software & Services$150M

Consumables$175M

ApplicationsExpertise

$650M business with industry-leading capabilities

• IC, LC, GC

• LC multiplexing

• Comprehensive offering

• IC, LC, GC columns

• Vials, septas, fittings

• Solvents, eluents

• Gold-standard CDS

• Most comprehensive

service offering

• >25 global application

and demo centers

• Workflow / methods

development expertise

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Broadest Range of HPLC/UHPLC on the Market

From entry level systems, to solutions designed for high throughput

Transcend UHPLC SolutionsFor multiplexed workflows

Dual LC SystemsTwo systems in one

UHPLC Systems1000 to 1250 Bar

Standard SystemsRange of detectors

Nano LC SolutionsVersatile or dedicated

ASMS 2011: More Capabilities, New Possibilities in Mass Spectrometry

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Thermo Scientific TSQ Quantum XLS Ultra

New standard in GC-MS/MS robustness and performance

• Environmental, food safety,

forensics, metabolomics

and pharmaceutical labs

• Low level analysis of highly

chlorinated compounds

• HyperQuad™ technology

delivers Ultra Selective

Reaction Monitoring (U-SRM)

• Increased matrix selectivity

• Unsurpassed sensitivity, and

easy detection

What it provides

Application Optimized

Best in class GC-MS/MS selectivity, analytical performance and productivity

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• No compromise MS and MS/MS

• Sensitivity, resolution, mass

accuracy and dynamic range, all

available at the same time

Orbitrap: The Performance Leader

What Orbitrap provides

Superior analytical results vs. TOF technology

Unmatched high resolution, accurate mass performance

Applications Optimized

• Virtually all molecular analyses:

identification, quantification,

qualification

• Proteomics, drug discovery,

environmental, food safety,

toxicology, forensics markets

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2000 2003 2005 20081999 2011

Dallas

Proof-of-the-principle for the

Orbitrap mass analyzer

Denver

Exactive benchtop

LC-Orbitrap launch

Long Beach

Resolving power >100,000

demonstrated

on concept instrument

Orbitrap Journey: Extending MS Leadership

Chicago

Orbitrap with external

accumulation developed

LTQ launch

San AntonioFirst Commercial

LTQ Orbitrap launch

PhiladelphiaLTQ Orbitrap Velos

2009

DenverOrbitrap Velos Pro

Orbitrap Elite

Q Exactive

Patent filing date:

March 31-1995 in

the UK. Granted

March 23-1999 in

USA US5,886,346

ASMS 2011: The biggest launches in Orbitrap history

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Orbitrap Platform Continues to Redefine the Mass Spec Market

Replaced $100M FT

Market for High-End Research

Displacing Triple Quads

in $700M Market

Entering the $250M

QTOF Market

• Approaching FT performance

at significantly lower cost

• Makes high resolution and

accurate mass accessible to

broader population

• Eliminates FT complications

• Unmatched speed, resolution,

sensitivity profile

• Redefining qual / quan workflows

• Priced to win

• Enables non-targeted screening

• Simultaneous qual / quan analysis

• Lowest cost / sample

Gaining share in $2B high-growth market

2008 20112005

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Orbitrap 2011: Delivering the Future

Parameter 2010 2011 Actual

Max. resolving power @ m/z 400 100,000 >240,000

Resolving power in 1 sec @ m/z 400 60,000 >240,000

External mass accuracy stability, days 1 1

Internal mass accuracy r.m.s., ppm 1 1

Dynamic range in 1 sec 5,000 8,000

Max. scan speed, spectra/second 4 10

Mass limit of isotopic resolution, kDa 30 50

Furthering the gap between Orbitrap and TOF technology

@ Future ASMS:

• Resolutions

>1,000,000

• Mass accuracy

of 0.2 ppm

• All have been

demonstrated!

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Stronger Leading Position in Mass Spectrometry

Our best ASMS ever!

Velos Pro: Extending Ion

Trap Leadership

Orbitrap Elite: Quantum

Leap in Performance

Q Exactive: Revolutionary

“Quanfirmation” Instrument

• Metabolite ID, peptide sequencing

• Fastest and most sensitive ion trap on market

• Ideal hybrid front-end

• Metabolite profiling, peptide sequencing, toxicology

• Sensitivity equal to high performance triple quads

• Identify, quantify and confirm in one analysis

• Proteomics, detailed structural analysis

• High field Orbitrap most significant advance since first Orbitrap

• Resolution and scan speed 4X current Orbitrap

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Velos Pro: Raising the Bar in Ion Trap Performance

• Fastest: first ion trap with 66,000

amu/sec. scan speed, 2X improvement

• New high dynamic range detection

system dramatically enhances

peptide/small molecule quantitation

• New Generation II ion optics for

improved robustness

• Unique Trap HCD fragmentation, gives

triple quad like fragments for improved

molecule structural identification

Ultimate in ion trap speed, sensitivity and flexibility

The Fastest, Most Sensitive Ion Trap in the World

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Orbitrap Elite: The New Performance Standard

• Resolving power of > 240,000

• 4X Scan speed improvement

• Revolutionary advanced signal processing

• New secondary detection system for

enhanced dynamic range

• Multiple fragmentation technologies for

comprehensive peptide and protein

identification; unambiguous small molecule

identification

• Ultimate top down protein analysis

A quantum leap in ultra high resolution and accurate mass analysis

New High Field Orbitrap provides unprecedented mass

resolution

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The First Benchtop Quadrupole Orbitrap

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Q Exactive: Redefining Qual/Quan Workflows

• 140,000 resolution benchtop Orbitrap

• New high performance quadrupole

parent ion selection capability

• New S Lens ion optics 3X sensitivity

• Higher MS/MS sensitivity and data

quality versus QTOF

• Targeted & non-targeted screening

• 3 workflows in one analytical run

Simultaneously identify, quantify and confirm:“Quanfirmation “

“Quanfirmation”: Simultaneously identify, quantify and

confirm

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New Software at ASMS

New leading edge software for challenging MS workflows

MetQuest 1.0

• For HR/AM qual/quan metabolic screening

• Unbiased full-scan data reveals unexpected metabolites

Tracefinder 1.1

• Automate environmental, food safety & clinical workflows

• Supports Triple Quad, Ion Trap and Exactive experiments

Xcalibur 2.1

• Core software control for all Thermo Scientific MS systems

• Now supports TLX/multiplexing and Turboflow automation

Exactfinder 1.0

• Integrated HR/AM qual/quan workflow analysis

• Targeted and non targeted screening

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Recap: New Platform Introductions at ASMS 2011

From the leader in mass spectrometry innovation

Quantum XLS Ultra GC-MS/MS

• For low level quantitation of extremely complex matrices

• Especially suited for highly chlorinated compounds (POPs)

Q-Exactive

• “Quanfirmation”: Identify, quantify and confirm simultaneously

• 140,000 resolution, 2X speed and 3X sensitivity improvement

Orbitrap Elite

• Resolution > 240,000 FWM at m/z 400 with eFT

• 4 x Scan speed improvement

Velos Pro/Orbitrap Velos Pro

• Linear quantitation, up to 6 orders of magnitude

• Fast scanning up to 66,000 Da/sec, compatible with UHPLC

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New Proteomics Tools at ASMS

Proteome Discoverer 1.3

• Comprehensive mass informatics platform for data mining

• Integrate biological information from multiple sources

Seive 2.0

• For label-free, semiquantitative, differential expression

• Expanded to support metabolomic & lipodomic workflows

Pinpoint 1.2

• Enhances speed and confidence in targeted quantitation.

• Supports multiple experimental approaches including HRAM

Thermo Scientific Pierce Enrichment Kits

• Enrich, capture and identify proteins, peptides, kinases

• Peptide Calibration kits and libraries, ActivX kinase probes

Easy-nLC 1000

• Split-free, nano-flow LC optimized for biomolecule separation

• Effortless ultra high pressure operation (1000 bar, 15,000 psi)

Leading edge tools for proteomics research

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In Conclusion…

• Dionex acquisition: comprehensive HPLC

portfolio complements leading MS technologies

• Ion trap leadership: extended with high speed

quantitative Velos Pro

• High field Orbitrap Elite: with >240,000

resolution, most significant advancement since

first Orbitrap

• Q Exactive: Revolutionary “Quanfirmation:”

screen, identify, confirm and quantify in one run

• Workflow based software: comprehensive new

suites for all applications

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Before You Leave Us

• We need two things from

you….

• Profile Sheet - Update us with

any missing contact details

• Feedback Form - Tell us how we

did!

Return these two sheets to a member of staff

and receive a souvenir USB Scientist to take

home with you

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Enjoy The Event!