Week 1: Orientation

• Orientation• Biological safety• Chemical safety• Fire safety

• Lab glassware• Pipetting

MEDT 251

• Goals• Schedule• Faculty• Policies• NiceNet class• Objectives: cognitive, psychomotor, affective• Offices and faculty• Resources

Pipets

• Transfer fluids• Make dilutions• Make primary and secondary standards• Reconstitute reagents and controls

Why so many kinds?

Pipet Variation…

• Types of fluid• Blood, plasma, serum• Aqueous reagents• Viscosity

• Degree of accuracy needed• Accuracy vs. efficiency

• Amount of fluid

Markings on Pipet

• Total volume and increments• Calibration temperature: 20oC• TD: to deliver• TC: to contain• Tolerance limits

Etched band means “blow out”

Color bands just indicate size

General Types of Pipet

• Volumetric: for accuracy• Volumetric transfer• Ostwald

• Graduated: for efficiency• Serological• Mohr

Pipetting1. Select the right pipet2. Check for broken tip3. Aspirate with bulb4. Wipe the tip5. Adjust meniscus6. Deliver along side of vessel7. Place in wash receptacle

Checking…

No!

Tip is completely in the fluid

Wipe the tip after aspiration

Dispense fluid along side of vessel

Division Safety Rules

• Read the Division’s safety manual

• Sign the Assumption of Risk and Release form