Analytical LCUNCOMPROMISED LC PERFORMANCE, NO MATTER WHERE THE SCIENCE TAKES YOU

They are compliance-ready compatible with Waters’ informatics suite, and deliver the accuracy, precision, linearity, and sensitivity that you require to meet your critical method requirements. Each system has a suite of column management, sample management, and detection capabilities that are optimized for their targeted application space.

WATERS’ PORTFOLIO OF ANALYTICAL LC SYSTEMS IS DESIGNED TO MEET YOUR REQUIREMENTS FOR RELIABILITY, ROBUSTNESS, AND REPEATABILITY

With fully scalable chemistries that can be used across any LC categories, you can transfer, and even adjust, your methods to meet your needs in any laboratory within your global operation. And with a worldwide support organization, we’re there to ensure your laboratory’s success from day one.

Discover how Waters’ analytical LC technologies can help you improve the quality and consistency of your analytical results, streamline your workflow, advance your scientific discovery, and improve your bottom line.

Adopting new liquid chromatography (LC) technology in your laboratory is more than just making a decision on which system will help you meet your method requirements. It’s about finding a technology that helps you solve your critical problems, achieve your business goals, and drive your organization forward.

As LC has evolved over recent years, the continued need for different levels of performance has become evident. The performance that best matches your laboratory depends upon the complexity of the samples, the number of samples analyzed each day, and the time-to-result required by your process.

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SYSTEM SCALABILITY: Matching LC columns to maximize performance

With a family of fully scalable column chemistries, you can easily transition a method across platforms, using the LC system that best suits your laboratory’s need. And with tools like the Columns Calculator, it’s easy to ensure you get the same results regardless of the LC system you deploy.

AU

0.000

0.010

0.020

0.030

0.040

0.050

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.75

AU

0.000

0.010

0.020

0.030

0.040

0.050

2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00

XBridge™ BEH C18, 5 µm, 4.6 x 150 mmFlow rate = 1.0 mL/minSolvent consumed = 15 mL/injection

XBridge BEH C18, 2.5 µm, 3.0 x 75 mmFlow rate = 0.85 mL/minSolvent consumed = 3.2 mL/injection

ACQUITY UPLC™ BEH C18, 1.7 µm, 2.1 x 50 mmFlow rate = 0.61 mL/minSolvent consumed = 1.0 mL/injection

Alliance™ HPLC

ACQUITY™ Arc™

ACQUITY UPLC H-Class PLUS

AU

0.000

0.010

0.020

0.030

0.040

0.050

min0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50 1.60 1.70

Rs = 2.0

Rs = 2.0

Rs = 2.0

PcAve .

W@50% 122

PcAve .

W@50% 118

PcAve .

W@50% 116

min

min

By matching the column attributes with the appropriate system characteristics, the chromatographic results (Resolution [Rs] and Average Peak Capacity [PCAve ] ) for this mixture of steroids remain constant, but the overall run times and cost per analysis decreases as the method is scaled from HPLC to UPLC.

ALLIANCE HPLC SYSTEM: DEFINED BY DEPENDABILITY, TODAY AND IN THE FUTURE

■■ Do you perform routine HPLC analysis?

■■ Are you looking for LC tools to streamline your daily workflow?

■■ Do you receive validated methods from other laboratories?

■■ Do you struggle to meet suitability requirements on your existing methods?

How the Alliance HPLC System can help you improve results and workflow:

■■ Easily transfer existing HPLC methods to the Alliance HPLC System.

■■ Simplify your daily LC system start-up with SystemPREP functionality.

■■ Automate your routine analysis with a fully compliant chromatography data system. Put the sample in the system, receive the results by email, and the Alliance HPLC System with Empower™ 3 Software will do the rest.

■■ A history of continued system improvements ensures the highest in HPLC performance, as well as the confidence that your results will be the same today as they were on a system manufactured 10 years ago, or on one manufactured in the future.

HPLC

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UHPLC

ACQUITY ARC SYSTEM: VERSATILITY WITHOUT COMPROMISE

■■ Do you perform routine HPLC analysis?

■■ Do you transfer chromatographic methods between laboratories and other global facilities?

■■ Are you considering updating your existing HPLC systems, but want to invest in a technology that can support UHPLC methods in the future?

■■ Are you looking for tools to improve laboratory efficiency?

How the ACQUITY Arc System can help you improve your results and workflow:

■■ Easily reproduce methods from existing Waters or other vendors’ HPLC systems.

■■ Arc Multi-flow path™ Technology enables simplified method transfer through a selectable dwell volume – not only can traditional HPLC methods be replicated, but new 2.x µm UHPLC methods can be developed to improve productivity.

■■ Deploy UHPLC methods using 2.7 µm CORTECS™ and XP chemistries on the ACQUITY Arc System to reduce cost per sample as compared to HPLC.

■■ Blend your mobile phase online with Auto•Blend Plus™ Technology to reduce errors both in preparation and in day-to-day and user-to-user variability.

■■ Bio-inert option (ACQUITY Arc Bio) for your routine bioseparations challenges.

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UPLC

ACQUITY UPLC PLUS SERIES: PERFORMANCE THAT ADVANCES YOUR LABORATORY

■■ Do you perform routine HPLC and/or UHPLC analysis and experience sample throughput bottlenecks that prevent you from getting timely answers to your business units?

■■ Do you analyze complex samples that require increased chromatographic resolution?

■■ Do you perform method development, or are you considering method modernization?

■■ Is LC-MS a key analytical tool for identification and/or characterization in your laboratory?

How the ACQUITY UPLC H-Class PLUS and ACQUITY UPLC I-Class PLUS Systems can help you improve your results and workflow:

■■ Run your existing LC methods while developing new UPLC methods for challenging analyses that require increased separation power.

■■ Auto•Blend Plus Technology provides online pH blending of buffers from pure solvents to support method development, method robustness testing, and LC-MS separation tuning (ACQUITY UPLC H-Class PLUS Quaternary System), yielding more efficient and robust methods.

■■ Combine the proven reliability and performance of the ACQUITY UPLC H-Class PLUS System with the precision and lowest system volume offered by the high pressure mixing binary solvent manager for challenging samples and high throughput applications (ACQUITY UPLC H-Class PLUS Binary System).

■■ Software and hardware tools support high-throughput applications, including inject- to-inject cycle times of less than 15 seconds (ACQUITY UPLC I-Class PLUS System).

■■ Optimal inlets for mass spectrometry.

■■ Bio-inert option (ACQUITY UPLC H-Class PLUS Bio) for your most challenging bioseparations and capacity as high as 7296 samples.

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HPLC

UHPLC

UPLC

HPLC<6000 psi

UHPLC6000 –

15,000 psi

UPLC9000 –

15,000 psi

UPLCtypically

<1 mL/min

DISPERSION IMPACT

OPTIMAL PARTICLE SIZE COST PER SAMPLE OPTIMAL COLUMN I.D.

TYPICAL OPERATING PRESSURE FLOW RATE

HPLCtypically

<3 mL/min

HPLC≥3.5 µm

UHPLC1.5–5 µm

UPLC<2 µm

HPLC UHPLC UPLC

HPLC≥4.6 mm

UHPLC2.1– 4.6 mm

UPLC1–3 mm

UHPLCtypically

<2 mL/min

DISPERSION MATTERS: Rethinking LC separation categories

Dispersion – n. Broadening of an analyte band due to both on-column effects (diffusion and mass transfer kinetics which are both dependent on particle size and linear velocity) and system effects (tubing internal diameter [I.D.] and length, connections, detector flow cell volumes, etc.).

True separation performance is governed by the system dispersion, paired with a flow rate or linear velocity that yields the highest possible efficiency for a given analytical column. As the system dispersion increases, the column volume must be proportionately increased, or a loss in chromatographic resolution will occur. Systems can be categorized based upon the column attributes that are best suited to match their dispersion characteristics.

Waters, The Science of What’s Possible, ACQUITY, Arc, Arc Multi-flow path, Alliance, Auto•Blend Plus, CORTECS, Empower, XBridge, and UPLC are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2019 Waters Corporation. Printed in the U.S.A. May 2019 720005394EN LM-PDF

For your local sales office, please visit www.waters.com/contact

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