Waterborne Norovirus Detection Using a Smartphone App and ......Project evolution- smartphone design...
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UNIVERSITY OFARIZONA Water & Energy Sustainable Technology Center WEST Center Research Waterborne Norovirus Detection Using a Smartphone App and Paper Microfluidics Jeong-Yeol Yoon (UA-BME), Kelly Reynolds (UA-COPH) and Soo Chung (UA-BME) Objectives: • Improve method sensitivity and usability • Field test prototype • Evaluate microfluidic paper types Key Deliverables: • Presented technology at ACS, resulting in ACS press release and broad media attention • Improved design with fabricated 3D printed enclosure • Identified select paper manufacturers • Conducted initial field comparisons Approach: • Fabricate a new 3D printed enclosure for improved usability and consistency of detection • Test multiple paper manufacturers for consistent results- additional calibration efforts underway • Develop an additional prototype for increasing field testing capacity • Duplicate assays for comparison between laboratories with different users/experience levels Project Dates: July 1, 2019-June 30, 2020 Rationale: • Norovirus is a primary cause of food and waterborne infections in the U.S. and the cause of an estimated 20 million acute intestinal infections per year. • Given virus low infectious dose, detection goals are a single viral unit. • Needed are real-time monitoring tools for rapid detection of norovirus in drinking water. Funding Source: Tucson Water and NSF-WET Center
Transcript of Waterborne Norovirus Detection Using a Smartphone App and ......Project evolution- smartphone design...
UNIVERSITY OFARIZONA
Water & Energy Sustainable Technology Center
WEST Center Research
Waterborne Norovirus Detection Using a Smartphone App and Paper Microfluidics
Jeong-Yeol Yoon (UA-BME), Kelly Reynolds (UA-COPH) and Soo Chung (UA-BME)
Objectives:
• Improve method sensitivity and usability• Field test prototype• Evaluate microfluidic paper types
Key Deliverables:
• Presented technology at ACS, resulting in ACS press release and broad media attention
• Improved design with fabricated 3D printed enclosure• Identified select paper manufacturers• Conducted initial field comparisons
Approach:
• Fabricate a new 3D printed enclosure for improved usability and consistency of detection
• Test multiple paper manufacturers for consistent results-additional calibration efforts underway
• Develop an additional prototype for increasing field testing capacity
• Duplicate assays for comparison between laboratories with different users/experience levels
Project Dates: July 1, 2019-June 30, 2020
Rationale:
• Norovirus is a primary cause of food and waterborne infections in the U.S. and the cause of an estimated 20 million acute intestinal infections per year.
• Given virus low infectious dose, detection goals are a single viral unit.
• Needed are real-time monitoring tools for rapid detection of norovirus in drinking water.
Funding Source: Tucson Water and NSF-WET Center
Smartphone Norovirus Detection in Water using Paper Microfluidics
Soo Chung, Jeong-Yeol Yoon,Agriculture and Biosystems Engineering
Kelly A. Reynolds
WEST Center Industry MeetingNovember 18, 2019
Tucson, AZ
Objectives
Study objective: Develop and apply an in-field smartphone technology for the rapid detection of noroviruses in water.
Specific aims:
• Evaluate intact norovirus limit of detection from drinking H2O outbreak samples and feces
• Field test prototype in various water types with utility personnel
• Prototype improvement- one smartphone and increased linear detection range
Project evolution- norovirus stocks
• Initial experiments focused on noninfectious norovirus capsids • Assay sensitivity uncertain relative to real-world samples
• Subsequent studies utilized intact noroviruses from well water samples following an outbreak• Genome copies from environmental samples my have high particle to infectious
particle ratios, compromising assay sensitivity assessment.
• Recent studies utilized fecal samples collected from infected individuals following outbreak• Concentrations ranged from 4.26×105 – 2.77×1010 genome copies per gram of
sample
Project evolution- smartphone design
• Initial design- two smartphones needed
• New design: improved optics, slide-in sample window, & single smartphone
Project evolution- smartphone design
Specificity test
The result with three different concentration of Zika virus tested with norovirus antibody-conjugated particles.
Norovirus in DI water
Norovirus- tap water
Norovirus- wastewater
ACS presentation and Peer-reviewed publication
A media storm
1. Arizona Daily Star. UA researchers create smartphone microscope to test for virus in water. September 16, 2019.
2. Food Safety News. Researchers develop method to detect low levels of norovirus. September 13, 2019
3. Bio5 Institute News. Can a smartphone stop a norovirus outbreak? September 13, 2019.
4. Hackaday. Norovirus smartphone: using megapixels and microfluidics to fight the other kind of virus infection. September 10, 2019
5. Medical News Today. Using a smartphone to detect a highly contagious virus. September 4, 2019
6. Forbes. Is norovirus in the water? Can a smartphone help? September 1, 2019
7. Biospace. Research roundup: IDing norovirus using a smartphone app. August 30, 2019
8. Futurity. Phone and paper detect norovirus with tiny beads. August 30, 2019
9. Interlochen Public Radio. A speedy test for norovirus could help water supplies check for contamination. August 30, 2019.
10. KVOA Tucson News. UA researchers use smartphone to detect noroviruses. August 30, 2019
11. Texas Public Radio. August 30, 2019
12. Physics World. Smartphone-based device detects norovirus. August 30, 2019
13. NPR Health. A speedy test for norovirus could help water supplies check for contamination. August 30, 2019
14. UANow. Science and Technology. Using a smartphone to detect norovirus. August 29, 2019
15. Chemistry World. Can a smartphone stop a norovirus outbreak? August 29, 2019.
16. International Business Times. Cruise Ship News: Scientists develop smartphone-based norovirus detector for cruise vacations. August 28, 2019
17. European Hospital. Using smartphones to detect norovirus. August 28, 2019
18. Medical Device Network. Smartphone device uses fluorescent beads to detect norovirus. August 28, 2019
19. Innovators Magazine. Quick detection stops norovirus. August 28, 2019
20. EurekaAlert! Using a smartphone to detect norovirus. AAAS
21. New Atlas. Simple smartphone-based system detects norovirus in water. August 27, 2019
22. UANews. Using a smartphone to detect norovirus August 27, 2019
23. ContagionLive. Norovirus detection goes mobile with new portable smartphone-based device August 27, 2019
24. SciTechDaily. Using a smartphone to detect norovirus (winter vomiting bug). August 27, 2019
25. MedGadget. Smartphone and paper microfluidic device for norovirus detection. August 27, 2019
26. New Hampshire Public Radio (NHPR). A newer, faster way to detect norovirus in water supplies. August 27, 2019
27. MedIndia. Smartphone enables detection of norovirus: here’s how. August 27, 2019
28. New Scientist. A smartphone app can detect tiny amounts of norovirus in water. August 27, 2019
29. Daily Mail. Smartphone app and attachable microscope can detect outbreaks of norovirus for just £40. August 27, 2019
30. NPR News. Special Series: Joe’s Big Idea. A newer, faster way to detect norovirus in water.. August 27, 2019
31. Phys.org. American Chemical Society. Smartphone-based device for detecting norovirus, the ‘cruise ship’ microbe. August 27, 2019
32. Water & Energy Sustainable Technology Center Newsletter. New research uses novel smartphone-based technology to detect waterborne pathogens. July 8, 2019
Next steps
• Re-calibrate new microfluidic paper characteristics
• Test the newly designed platform for ease of use and detection sensitivity
• Evaluate blinded (seeded and non-seeded) water samples in a controlled field trial with UA public health staff and students on a variety of seeded water samples
• Test feasibility and efficacy of method with Tucson Water Utility personnel
• Other applications:• Tucson Water/NSF-WET PFAS• Government: USAID, US Army, CDC• Non-profit: Engineers without Borders• Industry: Water Utilities, Food Safety, Cruise Lines
Acknowledgements
• Jeong-Yeol Yoon, PhD
• Soo Chung, PhD
• Tucson Water
• NSF-WET Center
• Lane Breshears
• Walter Bentancourt, PhD
• Charles Gerba, PhD
• Christina Morrison
• Sean Perea
Kelly A. Reynolds, PhD
