WATCH THIS EXPERIMENT LIVE! · mix 2 teaspoons of detergent, 1 teaspoon 3 of salt, and 1/2 cup of...

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Cells are the basic unit of life and make up all plants, animals, and bacteria. Deoxyribonucleic acid, or DNA, is the molecule that controls everything that happens in the cell. DNA contains instructions that direct the activities of cells and, ultimately, the body. This activity will demonstrate how DNA can be isolated from a strawberry using common household materials. What you will need: ‘Protocol’ is a fancy word for ‘Experiment’ STRAWBERRY DNA EXTRACTION PROTOCOL Pour rubbing alcohol down the side of the cup. It should be the same amount as the strawberry liquid. Do not mix or stir. You have just isolated the DNA from the rest of the material contained in the cells of the strawberry. 8 Pull off any leaves on the strawberries that have not been removed yet. 1 Put the strawberries into the plastic bag, seal it, and gently smash it for about 2 minutes. Completely crush the strawberries. This starts to break open the cells and release the DNA. 2 In a plastic cup, make your DNA extraction liquid: mix 2 teaspoons of detergent, 1 teaspoon of salt, and 1/2 cup of water. 3 Add 2 tsp of the DNA extraction liquid into the bag with the strawberries. This will further break open the cells. 4 Reseal the bag and gently smash for another minute (avoid making too many soap bubbles). 5 Place the coffee filter inside the other plastic cup. Open the bag and pour the strawberry liquid into the filter. You can twist the filter just above the liquid and gentlly squeeze the remaining liquid into the cup. 6-7 Within a few seconds, watch the development of a white cloudy substance (DNA) in the top layer the above strawberry extract layer. 9 Tilt the cup and pick up the DNA using a plastic coffee stirrer or wooden stick. 10 Visit: https://www.youtube.com/watch?v=hOpu4iN5Bh4 What you will do: WATCH THIS EXPERIMENT LIVE! 1 re-sealable plastic bag 2 strawberries (fresh or frozen) 2 tsp dish detergent 1/2 cup water 2 plastic cups 1 coffee filter 1/2 cup COLD rubbing alcohol 1 coffee stirrer 1 scientist 1 tsp of salt Learn more at www.illumina.com/stem

Transcript of WATCH THIS EXPERIMENT LIVE! · mix 2 teaspoons of detergent, 1 teaspoon 3 of salt, and 1/2 cup of...

Page 1: WATCH THIS EXPERIMENT LIVE! · mix 2 teaspoons of detergent, 1 teaspoon 3 of salt, and 1/2 cup of water. Add 2 tsp of the DNA extraction liquid into the bag with the strawberries.

Cellsare the basic unit of life and make up all plants,animals, and bacteria.Deoxyribonucleic acid, or DNA,is the molecule that controls everything that happens in the cell. DNA contains instructions that direct the activities of cells and, ultimately, the body.This activity will demonstrate how DNA can beisolated from a strawberry using commonhousehold materials.

What you will need:

‘Protocol’ is a fancy word for ‘Experiment’

STRAWBERRYDNA EXTRACTIONPROTOCOL

Pour rubbing alcohol down the side of the cup. It should be the same amount as the strawberry liquid. Do not mix or stir. You have just isolated the DNA from the rest of the material contained in the cells of the strawberry.

8

Pull off any leaves on the strawberriesthat have not been removed yet.1

Put the strawberries into the plastic bag, seal it, and gently smash it for about 2 minutes. Completely crush the strawberries. This starts to break open the cells and release the DNA.2

In a plastic cup, make yourDNA extraction liquid:mix 2 teaspoons of detergent, 1 teaspoonof salt, and 1/2 cup of water.3

Add 2 tsp of the DNA extraction liquid into the bag with the strawberries. This will further break open the cells.4

Reseal the bag and gently smashfor another minute(avoid making too many soap bubbles).5

Place the coffee filter inside the other plastic cup. Open the bag and pour the strawberry liquid into the filter. You can twist the filter just above the liquid and gentlly squeeze the remaining liquid into the cup.

6-7

Within a few seconds, watch the development of a white cloudy substance (DNA) in the top layer the above strawberry extract layer.9

Tilt the cup and pick up the DNA usinga plastic coffee stirrer or wooden stick.10

Visit:https://www.youtube.com/watch?v=hOpu4iN5Bh4

What you will do:

WATCH THISEXPERIMENT LIVE!

1 re-sealableplastic bag

2 strawberries(fresh or frozen)

2 tspdish detergent

1/2 cupwater

2 plastic cups 1 coffee filter 1/2 cup COLDrubbing alcohol

1 coffee stirrer

1 scientist1 tsp of salt

Learn more at www.illumina.com/stem

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