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UV-B Radiation, Poplar

Phytochemistry & Herbivory

 Jefrey M. Warren

 John H. Bassman

Washington State University

EPA STAR Graduate Fellowship Program

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Ultraviolet Radiation

• Ozone Loss

• UVB Radiation !n"rease

• #hromo$hores%henoli"s& $roteins& '()

• *fe"ts'ire"t vers+s indire"t

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*fe"ts o, UVB Radiation

UV-B Absorbed

Altered

Growth

AlteredLitter

AlteredSecondaryChemistry

AlteredCarbonAllocation

AlteredMorphology

Shift inCompetitive

Balance

athogen!elations

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O-e"tives

• !denti,y efe"ts o, enhan"ed UVBradiation on Populus tricocarpa ,oliar

-io"hemistry/%S& "hloro$hylls& 0avonoids& n+trients& tannin

• 'o these UVB ind+"ed "hangesafe"t Chrysomela scripta her-ivory/

1ro2th rate& $re,eren"e

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Methodology UVB Lam$s

• *nhan"ed UVB lam$ systems!ndoor nonmod+lated& O+tdoor mod+lated

• !ndoor St+dy 'esign3 treatments 456& 76& 86 UVB ,or %+llman&

W)9: -lo";s

: trees

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Methodology =rees

• Harvest %arameters1ro2th& lea, area& lea, $lasto"hron inde>

• #hemistry%hotosynthesis 7:# 435 se" $+lse9

#hloro$hyll a& - 'M? e>tra"tion

?lavonoids H%L# 4ether& *) $+ri@"ation9

(itrogen& S+l,+r& #ar-on #om-+stion

 =annins #BBBS) -inding assay

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Methodology !nse"ts

• ?eeding $re,eren"e trialsSingle -eetle in $etri dish 2ith lea, dis;s

3 treatments& 35 re$s& 8: ho+rs

• 1ro2th rate trials

Re"ently hat"hed larva gro2n on singlelea, 

3 treatments& A5 re$s& 83 2ee;s

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"#$

"#%

"#&

"#'

(reatment

   S   )   *   *   (  +   !   *   *   (   !  a   t   i  o   ,  g     g

$/ UVB

0/ UVB

%/ UVB

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$

%

&

'

1

0$

0%

0&

L2 0 L2 3 L2 0"

Leaf lastochron 2nde4

      )   *   (   *   S   5   6

   (   )   7   S   2   S

   ,  8  m

  o   l   C   *   %   9  4  e   d  m  -   %  s  -   0

   .

$/-UVB

0/-UVB

%/-UVB

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"#:

:#$

'#:

L2 ;% L2 & L2 '-1 L2

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'

3

L2 " L2 : Mean L2

Leaf lastochron 2nde4

   2   6   S   7   C   (   B   2   *   M   A   S   S   ,  m

  g   .

$/-UVB

0/-UVB

%/-UVB

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$#03

$#0<

$#%0

$#%"

$#%:

$#%3

%3$ %1$ %

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0"

0:

03

L2 ;% L2 @& L2 '-1

Leaf lastochron 2nde4

   =  o   l   i  a  r

   (   A   6   6   2   6

   ,  m  g   (   A   7   

  g      ?  p  o  p   l  a  r   .

0/-UVB

%/-UVB

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$#$$&

$#$$1

$#$0%

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#on"l+sions

• !n"reased UVB radiation/

• !n"reased ,oliar nitrogen and$hotosynthesis

• !n"reased 0avonoids -+t de"reased tannins

• !n"reased #LB $re,eren"e < #LB gro2th rate