QR Code PURPOSE

Red blood cells (RBCs) are a very important part

of every human being.

Besides carrying oxygen, RBCs are playing a

pivotal role in different disorders ranging from

different types of anemia over inherited

membrane disorders to parasitic infections.

Additionally, neurological disorders can alter

membrane elasticity and thereby impairing

oxygen transport. Thus, RBCs are representing a

very interesting and important cell population as

biomarker carrier in several diseases.

Tools, to evaluate the morphology of RBCs as well

as expression and staining patterns of different

biomarkers is highly anticipated.

Our newly designed RBC chips for Chipcytometry

only need a few drops of blood to analyze these

parameters on a high amount of Red Blood Cells.

In parallel.

The iterative process of staining and bleaching

allows the detection of different markers on the

same cell. In addition, storage of RBCs on the chip

is possible for later analyzing of interesting

markers.

CONCLUSIONS

With analyzing and storage of RBCs on a chip we offer an

outstanding new tool for red blood cell research.

The new modified chip surface allows the recovery and storage

of red blood cells. Besides analyzing different RBC specific

surface markers (CD235a and CD238) , the detection of

erythroid precursors is possible. Additionally, other markers –

not necessarily specific for RBCs- are detected. Storage of RBCs

is possible up to 20 weeks, with only slight decrease of marker

expression.

As image generation and analysis of the results can be

performed automatically on a high number of cells there is

nearly no limit of throughput to reach statistical significance in

a study.

METHODS AND RESULTS

ZellSafe Microfluidic Chips were modified to contain a cell-adhesive surface suitable for RBC recovery. Heparinized blood was diluted and RBCs loaded on the chip. Biconcavity – a hallmark of RBCs – was retained after immobilization in the chip.

Expression of several markers was analyzed on fresh RBCs.

ZELLKRAFTWERK GmbH Deutscher Platz 5c · 04103 Leipzig Germany Phone: +49 (441) 3508 2403 Fax: +49 (511) 532 8725 E-Mail: info[at]zellkraftwerk.com

A DIAGNOSTIC TOOL FOR RED BLOOD CELL DISORDERS –

CHIPCYTOMETRY AS A NEW RBC ANALYSIS PLATFORM

Anke Hofmeister-Brix, Christian Hennig

ZELLKRAFTWERK GmbH, Germany

www.zellkraftwerk.com

Marker stability was analyzed after different time points.

Storage of RBCs does not impact bioconcavity. All markers were still detectable even after 25 weeks of storage.

RBCs stored with addition of PBMCs

RBC-Storage test 1 (without addition of PBMCs) M1030 -04-025

M1030 -04-025

W1130 -04-028