Vol. 258, No. 16 The Journal of August 25, 1983

Biological Chemistry Copyright 0 1983 by the American Society of Biological Chemists, Inc., 428 East Preston St., Baltimore, Md. 21202 U.S.A.

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CONTENTS*

COMMUNICATIONS

the &subunit of its own receptor. Somotomedin-C stimulates the phosphorylation of

Steven Jacobs, Frederick C. Kull, Jr., H. Shelton Earp, Marjorie E. Suoboda, Judson J. Van Wyk, and Pedro Cua- trecasas Genetic polymorphisms for a phenobarbital-induci- ble cytochrome P-450 map to the Coh locus in mice.

Protease-activated kinase I1 as the potential media- tor of insulin-stimulated phosphorylation of ribo- somal protein S6.

Expression of human Chinese hamster hypoxanthine- guanine phosphoribosyltransferase cDNA recombi- nants in cultured Lesch-Nyhan and Chinese hamster fibroblasts.

John Brennand, David S. Konecki, and C. Thomas Caskey Degradation of microinjected methylated and un- methylated proteins in hepatoma tissue culture cells.

Seasonal variations in different forms of pokeweed antiviral protein, a potent inactivator of ribosomes.

L. L. Houston, S. Ramakrishmn, and Mark A. Hermodson Insulin receptor down regulation in human erythro- cytes.

Edward F. Murray Scott W. Peterson, Amy L. Miller, Robin S. Kelleher, and

Sites of methyl esterification on the aspartate recep- tor involved in bacterial chemotaxis.

and Daniel E. Koshland, Jr. Thomas C. Terwilliger, E l e m Bogonez, Elizabeth A. Wang,

Daniel L. Simmons and Charles B. Kasper

Olga Perisic and Jolinda A . Traugh

Reuital Katznelson and Richard G. Kulka

The structure of two blood group A-active glyco- sphingolipids with 12 sugars and a branched chain present in the epithelial cells of rat small intestine.

Gunnar C. Hansson Leukotriene Cd binding to rat lung membranes.

Sheng-Shung Pong, Robert N. DeHaven, Frederick A. Kuehl, Jr., and Robert W. Egan

production and excretion by mutant murine T- Biochemical effects of dipyridamole on purine over-

lymphoblasts. Buddy Ullman and Kiran Kaur

9649 The 3'-5' proofreading exonuclease of bacteriophage T4 DNA polymerase is stimulated by other T4 DNA replication proteins.

Patricia Bedinger and Bruce M. Alberts 9657 Protonic inhibition of the mitochondrial oligomycin-

and autolyzing cardiac muscle. Possible mechanism sensitive adenosine 5'-triphosphatase in ischemic

for the mitigation of ATP hydrolysis under nonener- gizing conditions.

William Rouslin 9662 Kinetic studies of calcium release from sarcoplasmic

reticulum in uitro. Do Han Kim, S. Tsuyoshi Ohnishi, and Noriaki Ikemoto

9669 Isolation and functional characterization of the ac- tive light chain of activated human blood coagulation - factor XI.

Bouma, Daniele M. Kerbiriou, and John H. Griffin Fedde van der Graaf, Judith S. Greengard, Bonno N.

9676 The transferrin cycle and iron uptake in rabbit retic- ulocytes. Pulse studies using "Fe, '2"I-labeled trans- ferrin.

Marco-Tulio Nuiwz and Jonathan Gloss 9681 Kinetics of internalization and recycling of transfer-

cell line. Effect of lysosomotropic agents. rin and the transferrin receptor in a human hepatoma

Aaron Ciechanover, Alan L. Schwartz, Alice Dautry-Var- sat, and Harvey F. Lodish

9690 Sugar transport by the bacterial phosphotransferase system. Preparation of a fluorescein derivative of the glucose-specific phosphocarrier protein IIIG" and its binding to the phosphocarrier protein HPr.

Edward G. Jablonski, Ludwig Brand, and Saul Roseman 9700 The reaction of 8-mercaptoflavins and flavoproteins

with sulfite. Evidence for the role of an active site arginine in D-amino acid oxidase.

Paul F. Fitzpatrick and Vincent Massey 9706 Molecular weight of the functional unit of human

leukocyte, fibroblast, and immune interferons. Sidney Pestka, Bruce Kelder, Philip C. Familletti, John

A. Moschera, Robert Crowl, and Ellis S. Kempner 9710 Mixed type inhibition of the renal Na+/H* antiporter

by Li+ and amiloride. Evidence for a modifier site. Harlan E. lues, Victoria J . Yee. and David G. Warnock

Acylation of CDP-monoacylglycerol cannot be con- 9717 Identification by direct photoaffinity labeling of an firmed.

cell. altered phosphodiesterase in a mutant 549 lymphoma William Thompson and Richard T. Zuk

9624 Endogenous phosphates on liver glycogen synthase D and synthase I. Studies on the number and location.

Agnes W. H. Tan and Frank Q. Nuttall 9631 Activation of mouse peritoneal macrophages by lip-

opolysaccharide alters the kinetic parameters of the superoxide-producing NADPH oxidase.

Masataka Sasada, Michael J . Pabst, and Richard B. John- ston, Jr.

9636 Binding of cAMP derivatives to Dictyostelium dis- coideum cells. Activation mechanism of the cell sur- face cAMP receptor.

Peter J . M. Van Haastert and Erik Kien

Vincent E. Groppi, Florence Steinberg, Harvey R. Kaslow,

9724 A T P activation of parathyroid hormone cleavage cat- Naomi Walker, and Henry R. Bourne

alyzcd by cathepsin D from bovine kidney. Sreekumar Pillai, Robert Botti, Jr., and James E. Zull

9729 Reaction of dATP with N-methyl-N-nitrosourea in vitro.

Mary S. Baker and Michuel D. Topal 9733 Purification and properties of a pantetheine-hydro-

lyzing enzyme from pig kidney.

mussen, Jack Brown, Bonita W. Wyse, and R. Gaurth Han- Carl T. Wittwer, Daue Burkhard, Kirk Ririe, Randy Ras-

sen 9643 Binding of cAMP and adenosine derivatives to Dic- 9739 TTpe IC, a novel glycogenosis. Underlying mecha-

Robert C. Nordlie, Katherine A. Sukalski, Juan M. Murioz, tyostelium discoideum cells. Relationships of bind- ing, chemotactic, and antagonistic activities.

nlsm.

Peter J. M. Van Haastert and Jerry J. Baldwin

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in THE JOURNAL, 258, 1 (19831, and reprints may be obtained from the editorial office.

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9878

Kinetic analysis of nicotinate phosphoribosyltrans- ferase from yeast using high pressure liquid chro- matography.

Lewis S. Hanna, Susan L. Hess, and Donald L. Sloan Forskolin-activated adenylate cyclase. Inhibition by guanyl-5"yl imidodiphosphate.

Solid state 13C NMR study of collagen molecular dy- namics in hard and soft tissues.

Susanta K. Sarkar, Catherine E. Sullivan, and Dennis A. Torchia Hormonal regulation of transcription of rDNA. In- hibition of transcription during glucocorticoid-me- diated inhibition of proliferation of lymphosarcoma P1798 cells in culture.

Pyrroline-5-carboxylatestimulates the conversion of purine antimetabolites to their nucleotide forms by a redox-dependent mechanism.

The large subunit of the fatty acid oxidation complex from Escherichia coli is a multifunctional polypep- tide. Evidence for the existence of a fatty acid oxi- dation operon (fad AB) in Escherichia coli.

broblasts. Effect of glucose starvation and role of Regulation of hexose carriers in chicken embryo fi-

protein synthesis. K a z w Yamada, Loyal G. Tillotson, and Kurt J. Isselbacher

Integration of Fl and the membrane sector of the proton-ATPase of Escherichia coli. Role of subunit "b" (uncF protein).

Preparation of DNA polymerase a. ClCz by reconsti- tuting DNA polymerase a with its specific stimula- tory cofactors, ClCZ.

DNA polymerase a cofactors CICz function as primer

Thomas H. Hudson and John N. Fain

Alice H. Cavanaugh and E. Aubrey Thompson, Jr.

Grace Chao Yeh and James M. Phang

Song- Yu Yang and Horst Schulz

David S. Perlin, David N. Cox, and Alan E. Senior

Cynthia G. Pritchard and Melvin L. DePamphilis

recognition proteins.

Melvin L. DePamphilis Cynthia G. Pritchard, David T. Weaver, Earl F. B a d , and

Thermodynamic parameters governing interaction of EcoRI endonuclease with specific and nonspecific DNA sequences.

Paul Modrich Brian J. Terry, William E. Jack, Robert A. Rubin, and

The antithrombin-binding sequence in heparin. Iden- tification of an essential 6-0-sulfate group.

Ulf Lindahl, Gudrun Bdz.strom, and Lennart Thunberg Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme.

Devron R. Averett, Carol Lubbers, Gertrude B . Elion, and Thomas Spector Purification of N-acetyl-L-glutamate synthetase from rat liver mitochondria and substrate and acti- vator specificity of the enzyme.

partial characterization. A DNA primase from mouse cells. Purification and

Changes in (Na+ + K+)-ATPase activity associated with stimulation of thymocytes by concanavalin A.

Titus Narendra N . Tandon, Lynn A. Davidson, and Elwood 0.

Mouse submaxillary gland renin contains a nonco- valently attached fatty acid.

Studies on the mechanism of the stimulation of po- lymerase 11-catalyzed RNA synthesis by mercury

Tomoko Sonoda and Masamiti Tatibana

Ben Y. Tseng and Clarence A. Ahlem

Martin Poe and Jerrold M. Liesch

compounds. Eddie Su-En Chao and Gerald D. Frenkel

Biochemical characterization, integrity, and sided- ness of purified skeletal muscle triads.

Sidnev Fleischer Robert D. Mitchell, Pompeo Volpe, Philip Palade, and

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Kinetics of copper(I1) uptake by apoazurin in com- plexing media.

ton, and David L. Tennent J u d i t h A . B h z a k , David R. McMillin, Andrew T. Thorn-

Characterization of UDP-galactose:2-acetamido-2- deoxy-D-glucose 3B-galactosyltransferase from pig trachea. -

-

Bradley T. Sheares and Don M. Carlson Topographic studies of spin-labeled bacteriorhodop- sin. Evidence for buried carboxyl residues and im- mobilization of the COOH-terminal tail.

Jeffrey M. Herr, Rolf J. Mehlhorn, and Lester Packer Comparison of the macroscopic and single channel conductance properties of colicin E l and its COOH- terminal tryptic peptide.

James 0. Bullock, Fredric S. Cohen, John R. Dankert, and William A. Cramer The mechanism of oxidation of nitroalkanes by horseradish peroxidase.

Characterization of initial autophosphorylation events in rabbit skeletal muscle phosphorylase ki- nase.

Marita M. King, Thomas J. Fitzgerald, and Gerald M. Carlson Primary structure of allophycocyanin from the uni- cellular rhodophyte, Cyanidiurn caldariurn. The complete amino acid sequences of the a and B sub- units.

The amino acid sequence of the polypeptide segment which regulates membrane adhesion (grana stacking) in chloroplasts.

John E. Mullet Regeneration of the native bacteriorhodopsin struc- ture from two chymotryptic fragments.

Mei-June Liao, Erwin London, and H. Gobind Khorana

sis at the site between pyruvate and phosphoenolpy- Catecholamine stimulation of hepatic gluconeogene-

ruvate.

overproduction of phosphatidylserine synthase in A trans-acting regulatory mutation that causes

Escherichia coli.

chromatography of alveolysin, a sulfhydryl-acti- Selective purification by thiol-disulfide interchange

vated toxin of Bacillus alvei. Toxin properties and interaction with cholesterol and liposomes.

Calcium-dependent proteolysis occurs during platelet

David J . T. Porter and Harold J. Bright

Gwynneth D. Offner and Robert F. Troxler

Raymond S. Ochs and Henry A. Lardy

Carl P. Sparrow and Christian R. H. Raetz

Christiane Geoffroy and Joseph E. Alouf

aggregation. John E. B. Fox, Clifford C. Reynolds, and David R. Phillips

Enzymes hydrolyzing ApppA and/or AppppA in

diadenosine triphosphatase, diadenosine tetraphos- higher plants. Purification and some properties of

phatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds.

Hieronim Jakubowskt and Andrzej Guranowski Identification of DNA polymerases involved in DNA excision repair in diploid human fibroblasts.

Steven L. Dresler and Michael W . Lieberman Direct effect of insulin on the binding of calmodulin to rat adipocyte plasma membranes.

Robert R. Goewert, Nancy B. Klaven, and Jay M. Mc- Donald

the outer envelope membrane of spinach chloroplasts. Localization of polypeptides to the cytosolic side of

Jacques Joyard, Agnes Billecocq, Sue G. Bartlett, Maryse A. Block, Nam-Hai Chua, and Roland Douce Codon usage and mistranslation. I n vivo basal level misreading of the MS2 coat protein message.

Jack Parker, Timothy C. Johnston, Peter T. Borgia, Greg Holtz, Erik Remaut, and Walter Fiers

Subunit associations of (Na+ + K+)-dependent aden- 10013 Choline acetyltransferase. Purification procedure naine trinhnanhataw. Chemical cross-linking studies. and factors affecting chromatographic properties """ " _r""___- "" - - - ~ ~~~~ ~ ~~~ ~ ~ .

Amir Askari Sankaridrug M. Periyasamy, Wu-Hsiung HLang, and and enzyme stability.

Costantino Cozzari and Boyd K. Hartman

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Biosynthesis and glycosylation of the insulin recep- tor. Evidence for a single polypeptide precursor of the two major subunits.

Jose A. Hedo, C. Ronald Kahn, Masao Hayashi, Kenneth M. Yamada, and Masato Kasuga Genetic determination of sn-glycerol-3-phosphate dehydrogenase synthesis in Drosophila melanogas- ter. A cis-acting controlling element.

proteins associated with acetylcholine receptor-rich Characterization and localization of the M. = 43,000

membranes.

Regulation of the expression of genes encoding types I, 11, and 111 collagen during chick embryonic devel- opment.

brugghe, and Ira Pastan Glenn T. Merlino, Catherine McKeon, Benoit de Crom-

The effect of RNA secondary structure on the action of a nucleolar endoribonuclease.

Characterization of the receptor for platelet-derived growth factor on human fibroblasts. Demonstration of an intimate relationship with a 185,000-dalton substrate for the platelet-derived growth factor- stimulated kinase.

lation of plasma membrane sheets and identification Coating cells with colloidal silica for high yield iso-

of transmembrane proteins.

Effect of lipid particle size on association of apolipo- proteins with lipid.

Shoji Tajima, Shinji Yokoyama, and Akira Yamamoto Molecular cloning of mRNA from 3T3 adipocytes.

dehydrogenase and other differentiation-dependent Regulation of mRNA content for glycerophosphate

proteins during adipocyte development. Bruce M. Spiegelman, Margaret Frank, and Howard Green

Construction of a cloned library of adenovirus DNA fragments in bacteriophage M13.

Ribosomal protein L1 from Escherichia coli. Its role in the binding of tRNA to the ribosome and in elon- gation factor G-dependent GTP hydrolysis.

Identification of the 3'-ends of the two mouse mito- chondrial ribosomal RNAs. The 3'-end of 16 S ribo- somal RNA contains nucleotides encoded by the gene for transfer R N A ~ .

Clnyton Richard A. Van Etten, Jeffrey W. Bird, and David A .

Ca2+-ATPase membrane crystals in sarcoplasmic re- ticulum. The effect of trypsin digestion.

ATP-dependent calcium transport in plasma mem- brane vesicles from neutrophil leukocytes.

Jacquelin B. Shaffer and Glenn C. Bewley

Seth Porter and Stanley C. Froehner

Duone C. Eichler and Susan J. Eales

Carl-Henrik Heldin, Bo Ek, and Lars Ronnstrand

Larry K. Chancy and Bruce S. Jacobson

Faye A . Eggerding and William C. Pierce

Gernot Sander

Laszlo Dux and Anthony Martonosi

Duane L. Ochs and Peter W. Reed

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10128

10136

10144

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10159

10168

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10192

Age-dependent regulation of the polymorphic forms of a2,-globulin.

Bandana Chatterjee A;un K. Roy, T. Surend Nath, Nalini M. Motwani, and

Analysis of the 3' end of the human pro-a2(I) collagen gene. Utilization of multiple polyadenylation sites in cultured fibroblasts.

Jeanne C. Myers, Leon A. Dickson, Wouter J . de Wet, Michael P. Bernard, Mon-Li Chu, Maurizio Di Liberto, Guglielmina Pepe, Frank 0. Sangtorgt, and Francesco Ra- mzrez Assembly of a functional Fo of the proton-translocat- ing ATPase of Escherichia coli.

Dan-1 J. Klionsky, William S. A. Brusilow, and Robert D. SimonL

Physarumpolycephalum. I. Caz+/actin-dependent in- Ca2+-dependent actin-binding phosphoprotein in

hibition of its phosphorylation. Hiroshi Maruta, Gerhnrd Isenberg, Thomas Schrecken-

bach, Rupert Hallmann, Gunduln Risse, Toshiko Shibayama, and Juliane Hesse Ca2+-dependent actin-binding ghosphoprotein in Physarum polycephalum. 11. Ca +-dependent F-ac-

by phosphorylation of subunit b. tin-capping activity of subunit a and its regulation

Acute adrenocorticotropic hormone stimulation of adrenal corticosteroidogenesis. Discovery of a rap- idly induced protein.

Rick J. Krueger and Nanette R. Orme-Johnson Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii.

Korn John A. Hammer, I I I , Joseph P. Albanesi, and Edward D.

The interaction of F-actin with phosphorylated and unphosphorylated myosins IA and IB from Acan- thamoeba castellanii.

Joseph P. Albanesi, John A. Hammer, I I I , and Edward D. Korn Cloning of DNA complementary to cytochrome P- 450 induced by pregnenolone- 16a-carbonitrile. Characterization of its mRNA, gene, and induction response.

James P. Hardwick, Frank J . Gonzalez, and Charles B. Kasper Evidence for the presence of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin in fresh granulosa cells. Effects of follicle-stimulating hor- mone and cyclic AMP on cholesterol side chain cleav- age cytochrome P-450 synthesis and activity.

Siler Masters, and Evan R. Simpson Bruria Funkenstein, Michael R. Waterman, Bettie Sue

Hiroshi Maruta and Gerhard Isenberg

ADDITIONS AND CORRECTIONS Arginine 45 is a major part of the antigenic deter- minant of human Bz-microglobulin recognized by mause monoclonal antibody BBM.l. Vol. 258 (1983)

Peter Parham, Matthew J. Androlewicz, Nicholas J . 6179-6186.

Holmes, and Barry E. Rothenberg

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Vol. 258, No. 16 The Journal of Biological Chemistry August 25,1983

CONTENTS Arranged by Subject Categories

CARBOHYDRATES. LIPIDS. AND OTHER NATURAL 9850 Changes in (Na+ + K+I-ATPase activitv associated

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' PRODUCTS The structure of two blood group A-active glyco- sphinogolipids with 12 sugars and a branched chain present in the epithelial cells of rat small intestine.

Acylation of CDP-monoacylglycerol cannot be con- firmed.

The antithrombin-binding sequence in heparin. Identification of an essential 6-0-sulfate group.

Ulf Lindahl, Gudrun Btickstram, and Lennart Thunberg Characterization of UDP-galactose:2-acetamido-2- deoxy-D-glucose 3@-galactosyltransferase from pig trachea.

Effect of lipid particle size on association of apoli- poproteins with lipid.

Shoji Tajima, Shinji Yokoyama, and Akira Yamamoto

Gunnar C. Hansson

William Thompson and Richard T. Zuk

Bradley T. Sheares and Don M. Carlson

CELL BIOLOGY AND METABOLISM Somotomedin-C stimulates the phosphorylation of the @-subunit of its own receptor.

Marjorie E. Svoboda, Judson J . Van Wyk , and Pedro Steven Jacobs, Frederick C. Kull, Jr., H. Shelton Earp,

Cuatrecasas Protease-activated kinase I1 as the potential media- tor of insulin-stimulated phosphorylation of ribo- somal protein S6.

Degradation of microinjected methylated and un- methylated proteins in hepatoma tissue culture cells.

Revital Katznelson and Richard G. Kulka Leukotriene C, binding to rat lung membranes.

Sheng-Shung Pong, Robert N. DeHaven, Frederick A. Kuehl, Jr., and Robert W. Egan

overproduction and excretion by mutant murine T- Biochemical effects of dipyridamole on purine

lymphoblasts.

Binding of CAMP and adenosine derivatives to Dic- tyostelium discoideum cells. Relationships of bind- ing, chemotactic, and antagonistic activities.

ticulocytes. Pulse studies using 68Fe,'2"I-labeled The transferrin cycle and iron uptake in rabbit re-

transferrin.

Kinetics of internalization and recycling of trans- ferrin and the transferrin receptor in a human hep- atoma cell line. Effect of lysosomotropic agents.

Aaron Ciechanover, Alan L. Schwartz, Alice Dautry- Varsat, and Harvey F. Lodish Sugar transport by the bacterial phosphotransferase system. Preparation of a fluorescein derivative of the glucose-specific phosphocarrier protein IIIG*" and its binding to the phosphocarrier protein HPr.

Edward G. Jablonski, Ludwig Brand, and Saul Roseman

altered phosphodiesterase in a mutant S49 lym- Identification by direct photoaffinity labeling of an

phoma cell.

Naomi Walker, and Henry R. Bourne Vincent E. Groppi, Florence Steinberg, Harvey R. Kaslow,

ATP activation of parathyroid hormone cleavage catalyzed by cathepsin D from bovine kidney.

Sreekumar Pillai, Robert Botti, Jr., and James E. Zull

of purine antimetabolites to their nucleotide forms Pyrroline-5-carboxylate stimulates the conversion

by a redox-dependent mechanism.

Olga Perisic and Jolinda A. Traugh

Buddy Ullman and Kiran Kaur

Peter J. M. Van Haastert

Marco-Tulio N u k and Jonathan Gloss

Grace Chao Yeh and James M. Phang

with %timulation of thymocytes by concanavalin A.

Titus Narendra N. Tandon, Lynn A. Dauidson, and Elwood 0.

9956 Catecholamine stimulation of hepatic gluconeo-

olpyruvate. genesis at the site between pyruvate and phosphoen-

Raymond S. Ochs and Henry A . Lardy 9973 Calcium-dependent proteolysis occurs during plate-

Joan E. B. Fox, Clifford C. Reynolds, and David R.

9995 Direct effect of insulin on the binding of calmodulin

Robert R. Goewert, Nancy B. Klauen, and Jay M. Mc-

10020 Biosynthesis and glycosylation of the insulin recep- tor. Evidence for a single polypeptide precursor of the two major subunits.

Jose A. Hedo, C. Ronald Kahn, Masao Hayashi, Kenneth M. Yamoda, and Masato Kasuga

10159 Acute adrenocorticotropic hormone stimulation of adrenal corticosteroidogenesis. Discovery of a rap- idly induced protein.

let aggregation.

Phillips

to rat adipocyte plasma membranes.

Donald

Rick J. Krueger and Nanette R. Orme-Johnson 10187 Evidence for the presence of cholesterol side chain

cleavage cytochrome P-450 and adrenodoxin in fresh granulosa cells. Effects of follicle-stimulating hormone and cyclic AMP on cholesterol side chain cleavage cytochrome P-450 synthesis and activity.

Bruria Funkenstein, Michael R. Waterman, Bettie Sue Siler Masters, and Evan R. Simpson

ENZYMOLOGY

9624 Endogenous phosphates on liver glycogen synthase D and synthase I. Studies on the number and loca- tion.

Agnes W . H . Tan and Frank Q. Nuttall

9631 Activation of mouse peritoneal macrophages by lip- opolysaccharide alters the kinetic parameters of the superoxide-producing NADPH oxidase.

Masataka Sasada, Michael J. Pabst, and Richard B. Johnston, Jr.

9669 Isolation and functional characterization of the ac- tive light chain of activated human blood coagula- tion factor XI.

Fedde van, der Granf, Judi th S. Greengard, Bonno N. Bouma, Danleb M. Kerbrriou, and John H. Griffin

9700 The reaction of 8-mercaptoflavins and flavoproteins with sulfite. Evidence for the role of an active site arginine in D-amino acid oxidase.

Paul F. Fitzpatrick and Vincent Massey 9733 Purification and properties of a pantetheine-hydro-

lyzing enzyme from pig kidney.

Rasmussen, Jack Brown, Bonita W . Wyse, and R. Gaurth Carl T. Wittwer, Dave Burkhard, Kirk Ririe, Randy

Hansen 9739 TTpe IC, a novel glycogenosis. Underlying mecha-

nism. Robert C. Nordlie, Katherine A. Sukalski, Juan M. Mu-

iwz, and Jerry J. Baldwin 9745 Kinetic analysis of nicotinate phosphoribosyltrans-

ferase from yeast using high pressure liquid chro- matography.

Lewis S. Hanna, S w a n L. Hess, and Donald L. Sloan

9755 Forskolin-activated adenylate cyclase. Inhibition by guanyl-5'-yl imidodiphosphate.

Thomas H. Hudson and John N. Fain

5 Indicates a Communication

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The large subunit of the fatty acid oxidation complex from Escherichia coli is a multifunctional polypep- tide. Evidence for the existence of a fatty acid oxi- dation operon (fad AB) in Escherichia coli.

Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme.

Devron R. Averett, Carol Lubbers, Gertrude B. Elion, and Thomas Spector Purification of N-acetyl-L-glutamate synthetase from rat liver mitochondria and substrate and acti- vator specificity of the enzyme.

The mechanism of oxidation of nitroalkanes by horseradish peroxidase.

Characterization of initial autophosphorylation events in rabbit skeletal muscle phosphoylase ki- nase.

Marita M. King, Thomas J. Fitzgerald. and Gerald M. Carlson Enzymes hydrolyzing ApppA and/or AppppA in higher plants. Purification and some properties of diadenosine triphosphatase, diadenosine tetraphos- phatase, and phosphodiesterase from yellow lupin (Lupin? futeus) seeds.

Choline acetyltransferase. Purification procedure and factors affecting chromatographic properties and enzyme stability.

Purification and characterization of a myosin I heavy chain kinase from Acantharnoeba castellanii.

John A. Hammer, III, Joseph P. Albanesi, and Edward D. Korn The interaction of F-actin with phosphorylated and unphosphorylated myosins IA and IB from Acun- tharnoeba castellanii.

D. Korn Joseph P, Albanesi, John A. Hammer, I l l , and Edward

Song- Yu Yang and Horst Schulz

Tomoko Sonoda and Masamiti Tatibana

David J . T. Porter and Harold J . Bright

Hieronrm Jakubowski and Andrzej Guranowski

Costantino Cozzari and Boyd K. Hartman

MEMBRANES AND BIOENERGETICS Insulin receptor down regulation in human eryth- rocytes.

Scott W. Peterson, A m y L. Miller, Robin S. Kelleher, and Edward F. Murray Protonic inhibition of the mitochondrial oligomycin- sensitive adenosine 5‘-triphosphatase in ischemic and autolyzing cardiac muscle. Possible mechanism for the mitigation of ATP hydrolysis under non- energizing conditions.

William Rouslin

reticulum in uitro. Kinetic studies of calcium release from sarcoplasmic

Do Han Kim, S. Tsuyoshi Ohnishi, and Noriaki lkemoto Mixed type inhibition of the renal Na+/H+ antiporter by Li+ and amiloride. Evidence for a modifier site.

Harlan E. Ives, Vzctorla J. Yee, and Davzd G. Warnock Regulation of hexose carriers in chicken embryo fibroblasts. Effect of glucose starvation and role of protein synthesis.

K a z w Yanurda, Loyal G. Tillotson, and Kurt J. Issel- bacher Integration of F1 and the membrane sector of the proton-ATPase of Escherichia coli. Role of subunit “b” (uncF protein).

Biochemical characterization, integrity, and sided- ness of purified skeletal muscle triads.

Sidney Fleischer Robert D. Mitchell, Pompeo Volpe, Philip Palade, and

Subunit associations of (Na+ + K+)-dependent aden- osine triphosphatase. Chemical cross-linking stud- les.

Sankaridrug M. Periyasamy, Wu-Hsiung Huang, and Amir Askari

David S. Perlin, David N. Cox, and Alan E. Senior

9908 Comparison of the macroscopic and single channel conductance properties of colicin E l and its COOH- terminal tryptic peptide.

James 0. Bullock, Fredric S. Cohen, John R. Dankert, and William A. Cramer

9941 The amino acid sequence of the polypeptide segment which regulates membrane adhesion (grana stack- ing) in chloroplasts.

John E. Mullet 9949 Regeneration of the native bacteriorhodopsin struc-

Mei-June Liao, Erwin London, and H. Gobind Khorann 9963 A trans-acting regulatory mutation that causes

overproduction of phosphatidylserine synthase in Escherichia coli.

ture from two chymotryptic fragments.

Carl P. Sparrow and Christian R. H. Raetz 10000 Localization of polypeptides to the cytosolic side of

the outer envelope membrane of spinach chloro- plasts.

A. Block, Nam-Hai Chua, and Roland Douce Jacques Joyard, Agnes Bilkcocq, Sue G. Bartlett, Maryse

10034 Characterization and localization of the M, = 43,000 proteins associated with acetylcholine receptor-rich membranes.

Seth Porter and Stanley C. Froehner 10062 Coating cells with colloidal silica for high yield iso-

lation of plasma membrane sheets and identification of transmembrane proteins.

Larry K. Chaney and Bruce S. Jacobson

ticulum. The effect of trypsin digestion. 10111 Ca*+-ATPase membrane crystals in sarcoplasmic re-

Laszlo Dux and Anthony Martonosi 10116 ATP-dependent calcium transport in plasma mem-

brane vesicles from neutrophil leukocytes. Duane L. Ochs and Peter W. Reed

cating ATPase of Escherzchia coli.

D. Simoni

10136 Assembly of a functional, FO of the proton-translo-

Daniel J. Klionsky, WLlltam S. A . Brusilow, and Robert

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

99585

-9593

9649

9729

9768

9801

9810

9820

Genetic polymorphisms for a phenobarbital-induci- ble cytochrome P-450 map to the Coh locus in mice.

Expression of human Chinese hamster hypoxan- thine-guanine phosphoribosyltransferase cDNA re- combinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts.

John Brennand, David S. Konecki, and C. Thomas Cas- key The 3’-5’ proofreading exonuclease of bacterio- phage T4 DNA polymerase is stimulated by other T4 DNA replication proteins.

Reaction of dATP with N-methyl-N-nitrosourea in vitro.

Hormonal regulation of transcription of rDNA. In-

diated inhibition of proliferation of lymphosarcoma hibition of transcription during glucocorticoid-me-

P1798 cells in culture.

Preparation of DNA polymerase (I. C ~ C Z by recon- stituting DNA polymerase CY with its specific stimu- latory cofactors, CIC2.

DNA polymerase CY cofactors C1C2 function as primer recognition proteins.

Cynthia G. Pritchurd, David T. Weaver, Earl F. B a d , and Melvin L. DePamphilis

of EcoRI endonuclease with specific and nonspecific Thermodynamic parameters governing interaction

Daniel L. Simmons and Charles B. Kasper

Patricia Bedinger and Bruce M. Alberts

Mary S. Baker and Michael D. Topal

Atice H. Cauanaugh and E. Aubrey Thompson, Jr.

Cynthia G. Pritchurd and Melvin L. DePamphilis

DNA sequences.

Paul Modrrch Brian J . ,Terry, William E . Jack, Robert A . Rubin, and

Indicates a Communication

9845

9861

9990

10007

10027

10041

10049

10083

10090

10098

10104

10123

10128

10182

A DNA primase from mouse cells. Purification and partial characterization.

lymerase 11-catalyzed RNA synthesis by mercury Studies on the mechanism of the stimulation of po-

compounds.

Identification of DNA polymerases involved in DNA excision repair in diploid human fibroblasts.

Steven L. Dresler and Michael W. Lieberman Codon usage and mistranslation. In vivo basal level misreading of MS2 coat protein message.

Holtz, Erik Remaut, and Walter Fiers Jack Parker, T i m t h y C. Johnston, Peter T. Borgia, Greg

Genetic determination of an-glycerol-3-phosphate dehydrogenase synthesis in Drosophila melanogas- ter. A cis-acting controlling element.

Regulation of the expression of genes encoding types I, 11, and I11 collagen during chick embryonic devel- opment.

Glenn T. Merlino, Catherine McKeon, Benoit de Crom- brugghe, and Ira Pastan The effect of RNA secondary structure on the action of a nucleolar endoribonuclease.

Molecular cloning of mRNA from 3T3 adipocytes. Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation-dependent proteins during adipocyte development.

Green Bruce M. Spiegelman, Margaret Frank, and Howard

Construction of a cloned library of adenovirus DNA fragments in bacteriophage M13.

Ribosomal protein L1 from Escherichia coli. Its role in the binding of tRNA to the ribosome and in elon- gation factor G-dependent GTP hydrolysis.

chondrial ribosomal RNAs. The 3'-end of 16 S ri- Identification of the 3'-ends of the two mouse mito-

bosomal RNA contains nucleotides encoded by the gene for transfer R N A ~ & .

Richard A. Van Etten, Jeffrey W. Bird, and David A. Clayton

of a2,-globulin. Age-dependent regulation of the polymorphic forms

Arun K. Roy, T. Surend Nath, Nalini M. Motwani, and Bandana Chatterjee Analysis of the 3' end of the human pro-a2(I) colla- gen gene. Utilization of multipIe polyadenylation sites in cultured fibroblasts.

Michael P. Bernard, Mon-Li Chu, Maurizio Dz Lzberto, Jeanne C. Myers, Leon A. Dickson, Wouter J.. de- Wet,

G@ielmina Pepe, Frank 0. Sangiorgi, and Francesco Ra- mzrez Cloning of DNA complementary to cytochrome P- 450 induced by pregnenolone- 16a-carbonitrile. Characterization of its mRNA, gene, and induction

Ben Y. Tseng and Clarence A. Ahlem

Eddie Su-En Chno and Gerald D. Frenkel

Jacquelin B. Shnffer and Glenn C. Bewley

Duane C. Eichler and Susan J. Eales

Faye A. Eggerding and William C. Pierce

Gernot Sander

response.

Kasper James P. Hardwick, Frank J . Gonzalez, and Charles B.

*9601

-9608

9636

9706

9762

9856

9886

9899

9931

9968

10054

10144

10151

PROTEIN CHEMISTRY AND STRUCTURE

antiviral protein, a potent inactivator of ribosomes. Seasonal variations in different forms of pokeweed

L. L. Houston, S. Ramakrishnan, and Mark A. Hermod- son Sites of methyl esterification on the asparate recep- tor involved in bacterial chemotaxis.

Wang, and Daniel E. Koshland, Jr. Thomas C. Terwilliger, E l e m Bogonez, Elizabeth A .

coideum cells. Activation mechanism of the cell sur- Binding of CAMP derivatives to Dictyostelium dis-

face CAMP receptor.

leukocyte, fibroblast, and immune interferons. Molecular weight of the functional unit of human

Sidney Pestka, Bruce Kelder, Philip C. Familletti, John A. Moschera, Robert Crowl, and Ellis S. Kempner Solid state ''C NMR study of collagen molecular dynamics in hard and soft tissue.

Susanta K. Sarkar, Catherine E. Sullivan, and Dennis A . Torchia

Mouse submaxillary gland renin contains a nonco- valently attached fatty acid.

Kinetics of copper(I1) uptake by apoazurin in com-

Peter J. M. Van Haastert and Erik Kien

Martin Poe and Jerrold M. Liesch

plexing media. Judith A. Bloszak. David R. McMillin, Andrew T. Thorn-

ton, and David L. Tennent

Topographic studies of spin-labeled bacteriorhodop- sin. Evidence for buried carboxyl residues and im- mobilization of the COOH-terminal tail.

Jeffrey M. Herz, Rolf J . Mehlhorn, and Lester Packer

unicellular rhodophyte, Cyanidium caldarium. The Primary structure of allophycocyanin from the

complete amino acid sequences of the a and @ sub- units.

Gwynneth D. Offner and Robert F. Troxler

Selective purification by thiol-disulfide interchange chromatography of alveolysin, a sulfhydryl-acti- vated toxin of Bacillus alvei. Toxin properties and interaction with cholesterol and liposomes.

growth factor on human fibroblasts. Demonstration Characterization of the receptor for platelet-derived

of an intimate relationship with a 186,000-dalton substrate for the platelet-derived growth factor- stimulated kinase.

Physarum polycephalum. I. Ca2+/actin-dependent Cas+-dependent actin-binding phosphoprotein in

inhibition of its phosphorylation. Hiroshi Maruta, Gerhard Isenberg, Thomas Schrecken-

bach, Rupert Hallmann, Gundula Risse, Toshiko Shibay- ama, and Juliane Hesse

Physarum polycephalum. 11. Ca *-dependent F-ac- Cas+-dependent actin-binding phosphoprotein in

tin-capping activity of subunit a and its regulation by phosphorylation of subunit b.

Christiane Geoffroy and Joseph E. Alouf

Carl-Henrik HeMin, Bo Ek, and Lars RZtnnstrand

Hiroshi Maruta and Gerhard Isenbeg

Indicates a Communication