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LETTERS Hydatellaceae are water lilies with gymnospermous tendencies William E. Friedman 1 The flowering plant family Hydatellaceae was recently discovered to be allied to the ancient angiosperm lineage Nymphaeales (water lilies) 1 . Because of its critical phylogenetic position, members of the Hydatellaceae have the potential to provide insights into the origin and early diversification of angiosperms 2 . Here I report that Hydatella expresses several rare embryological features that, in combination, are found only in members of the Nymphaeales. At maturity, the female gametophyte is four-celled, four-nucleate and will produce a diploid endosperm, as is characteristic of most early divergent angiosperm lineages 3,4 . As with all members of the Nymphaeales, endosperm in Hydatella is minimally developed and perisperm is the major embryo-nourishing tissue within the seed 5,6 . Remarkably, Hydatella exhibits a maternal seed- provisioning strategy that is unique among flowering plants, but common to all gymnosperms 7 : pre-fertilization allocation of nutrients to the embryo-nourishing tissue. This exceptional case of pre-fertilization maternal provisioning of a seed in Hydatella may well be an apomorphic feature of Hydatellaceae alone but, given the newly discovered phylogenetic position of this family, potentially represents a plesiomorphic and transitional condition associated with the origin of flowering plants from gymnosper- mous ancestors. For over a century, the flowering plant family Hydatellaceae was thought to belong to the Poales, a highly derived monocot order that includes the grasses 1,2 . New evidence derived from DNA sequencing and phylogenetic analysis shows that this obscure group of minute aquatic plants is closely related to water lilies (Nymphaeales) 1 , which themselves were only recently discovered to be one of the most ancient extant lineages of angiosperms 8–12 . As a result of these sur- prising phylogenetic insights, our understanding of the earliest phases of the radiation of angiosperms continues to be very much in flux 3,13 . Although the data are, at best, ambiguous, the original embryolo- gical studies of Hydatellaceae 5,14,15 suggested that the female gameto- phyte might contain eight nuclei and seven cells, with antipodals that degenerate early (or are absent), and possibly two polar nuclei; as such, Hydatellaceae was thought to produce a triploid genetically biparental endosperm. Recently, however, members of the Nymphaeales and Austrobaileyales (another ancient lineage of flowering plants) have been shown to produce an unusual four-celled, four-nucleate female gametophyte (Nuphar/Schisandra-type) that lacks antipodal cells and a second polar nucleus (Fig. 1). Importantly, the target of the second fertilization event, the central cell of the female gametophyte, is hap- loid in Nymphaeales and Austrobaileyales, and endosperm in these ancient lineages is diploid, not triploid as in most flowering plants 3 . Because of the recent phylogenetic insights into what constitute the most ancient angiosperm lineages 1 , Hydatellaceae might be predicted to share some of the rare and potentially conservative embryological features of water lilies and other ancient lineages of flowering plants. Working with field-collected material of Hydatella inconspicua,I examined female gametophyte development and ontogenetic fea- tures of the formation of embryo-nourishing reserves in the ovule/ seed. The mature female gametophyte of H. inconspicua contains four uninucleate cells: the egg cell, two synergids and a uninucleate central cell (Fig. 2). No antipodal cells are evident, nor is a second polar nucleus present within the central cell. However, in many flowering plants with seven-celled, eight-nucleate female gametophytes (Polygonum-type), the three antipodal cells degenerate and the two haploid nuclei of the central cell fuse before the fertilization process 3 . The result is a female gametophyte that is structurally indis- tinguishable, at maturity, from a ‘true’ four-celled female gameto- phyte (Nuphar/Schisandra-type) that never forms antipodal cells or a second polar nucleus. To distinguish between these two types of female gametophyte, early developmental stages must be examined (Fig. 1). Critically, at the two-nucleate syncytial stage in H. inconspicua, both nuclei are located at the micropylar pole of the female gameto- phyte (Fig. 2a). This is precisely the pattern exhibited by other four-nucleate, four-celled gametophytes found in Nymphaeales and Austrobaileyales, and differs from the comparable ontogenetic stage of Polygonum-type (and Amborella-type 13 ) gametophytes where the nuclei at the two-nucleate syncytial stage are displaced 1 Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado 80309, USA. Nuphar/Schisandra-type Polygonum-type Figure 1 | Schematic of four-celled, four-nucleate (Nuphar/Schisandra- type) female gametophyte development in Nymphaeales and Austrobaileyales and of seven-celled, eight-nucleate (Polygonum-type) development in most other angiosperms. The micropylar pole is towards the top of the figure. At the two-nucleate syncytial stage (blue boxes), water- lily female gametophytes have both nuclei at the micropylar pole; in Polygonum-type female gametophytes, a nuclear migration event leads to placement of a single nucleus at each pole. Red, synergids; yellow, egg cell; brown, antipodals. cc, central cell. pn, polar nucleus. Vol 453 | 1 May 2008 | doi:10.1038/nature06733 94 Nature Publishing Group ©2008

Transcript of Vol 453 1 May 2008 doi:10.1038/nature06733 LETTERS

LETTERS

Hydatellaceae are water lilies with gymnospermoustendenciesWilliam E. Friedman1

The flowering plant family Hydatellaceae was recently discoveredto be allied to the ancient angiosperm lineage Nymphaeales (waterlilies)1. Because of its critical phylogenetic position, members ofthe Hydatellaceae have the potential to provide insights into theorigin and early diversification of angiosperms2. Here I report thatHydatella expresses several rare embryological features that, incombination, are found only in members of the Nymphaeales.At maturity, the female gametophyte is four-celled, four-nucleateand will produce a diploid endosperm, as is characteristic ofmost early divergent angiosperm lineages3,4. As with all membersof the Nymphaeales, endosperm in Hydatella is minimallydeveloped and perisperm is the major embryo-nourishing tissuewithin the seed5,6. Remarkably, Hydatella exhibits a maternal seed-provisioning strategy that is unique among flowering plants, butcommon to all gymnosperms7: pre-fertilization allocation ofnutrients to the embryo-nourishing tissue. This exceptional caseof pre-fertilization maternal provisioning of a seed in Hydatellamay well be an apomorphic feature of Hydatellaceae alone but,given the newly discovered phylogenetic position of this family,potentially represents a plesiomorphic and transitional conditionassociated with the origin of flowering plants from gymnosper-mous ancestors.

For over a century, the flowering plant family Hydatellaceae wasthought to belong to the Poales, a highly derived monocot order thatincludes the grasses1,2. New evidence derived from DNA sequencingand phylogenetic analysis shows that this obscure group of minuteaquatic plants is closely related to water lilies (Nymphaeales)1, whichthemselves were only recently discovered to be one of the mostancient extant lineages of angiosperms8–12. As a result of these sur-prising phylogenetic insights, our understanding of the earliestphases of the radiation of angiosperms continues to be very muchin flux3,13.

Although the data are, at best, ambiguous, the original embryolo-gical studies of Hydatellaceae5,14,15 suggested that the female gameto-phyte might contain eight nuclei and seven cells, with antipodals thatdegenerate early (or are absent), and possibly two polar nuclei; as such,Hydatellaceae was thought to produce a triploid genetically biparentalendosperm. Recently, however, members of the Nymphaeales andAustrobaileyales (another ancient lineage of flowering plants) havebeen shown to produce an unusual four-celled, four-nucleate femalegametophyte (Nuphar/Schisandra-type) that lacks antipodal cells anda second polar nucleus (Fig. 1). Importantly, the target of the secondfertilization event, the central cell of the female gametophyte, is hap-loid in Nymphaeales and Austrobaileyales, and endosperm in theseancient lineages is diploid, not triploid as in most flowering plants3.Because of the recent phylogenetic insights into what constitute themost ancient angiosperm lineages1, Hydatellaceae might be predictedto share some of the rare and potentially conservative embryologicalfeatures of water lilies and other ancient lineages of flowering plants.

Working with field-collected material of Hydatella inconspicua, Iexamined female gametophyte development and ontogenetic fea-tures of the formation of embryo-nourishing reserves in the ovule/seed. The mature female gametophyte of H. inconspicua contains fouruninucleate cells: the egg cell, two synergids and a uninucleate centralcell (Fig. 2). No antipodal cells are evident, nor is a second polarnucleus present within the central cell. However, in many floweringplants with seven-celled, eight-nucleate female gametophytes(Polygonum-type), the three antipodal cells degenerate and thetwo haploid nuclei of the central cell fuse before the fertilizationprocess3. The result is a female gametophyte that is structurally indis-tinguishable, at maturity, from a ‘true’ four-celled female gameto-phyte (Nuphar/Schisandra-type) that never forms antipodal cells ora second polar nucleus. To distinguish between these two types offemale gametophyte, early developmental stages must be examined(Fig. 1).

Critically, at the two-nucleate syncytial stage in H. inconspicua,both nuclei are located at the micropylar pole of the female gameto-phyte (Fig. 2a). This is precisely the pattern exhibited by otherfour-nucleate, four-celled gametophytes found in Nymphaealesand Austrobaileyales, and differs from the comparable ontogeneticstage of Polygonum-type (and Amborella-type13) gametophyteswhere the nuclei at the two-nucleate syncytial stage are displaced

1Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado 80309, USA.

Nuphar/Schisandra-type

Polygonum-type

Figure 1 | Schematic of four-celled, four-nucleate (Nuphar/Schisandra-type) female gametophyte development in Nymphaeales andAustrobaileyales and of seven-celled, eight-nucleate (Polygonum-type)development in most other angiosperms. The micropylar pole is towardsthe top of the figure. At the two-nucleate syncytial stage (blue boxes), water-lily female gametophytes have both nuclei at the micropylar pole; inPolygonum-type female gametophytes, a nuclear migration event leads toplacement of a single nucleus at each pole. Red, synergids; yellow, egg cell;brown, antipodals. cc, central cell. pn, polar nucleus.

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to opposite poles of the female gametophyte (Fig. 1). Thus, Hydatellaproduces a four-celled, four-nucleate female gametophyte thatprecisely matches (developmentally and at maturity) the uniqueand potentially plesiomorphic pattern found in two of the mostancient extant angiosperm lineages (Fig. 2c)16,17. The finding that

Hydatella produces a female gametophyte identical to those foundin the water lilies is clearly concordant with both the phylogeneticplacement of Hydatellaceae as sister to the previously recognizedNymphaeales and its general position among ancient extant angio-sperm clades.

cc

pn

ecsc

a b

Figure 2 | Female gametophyte development in Hydatella inconspicua.a, Two-nucleate syncytial female gametophyte in H. inconspicua, with bothnuclei (red arrowheads) at micropylar pole. Grey box contains digitalsuperposition of second nucleus from adjacent histological section.

b, Mature four-celled female gametophyte in H. inconspicua. Grey boxcontains digital superposition of second synergid nucleus from adjacenthistological section. cc, central cell; ec, egg cell; pn, polar nucleus; sc, synergidcell.

Figure 3 | Pre- and post-fertilization development of perisperm inH. inconspicua. a, Longitudinal section of perisperm in pre-fertilizationovule at two-nucleate stage of female gametophyte development. b, Cross-polarization optical image demonstrating presence of starch from boxedregion in a. Starch grains, which are birefringent, appear as four whitequadrants separated by a black cross. c, Longitudinal section of perisperm inpre-fertilization ovule just before fertilization. d, Cross-polarization opticalimage demonstrating presence of large amounts of starch from boxed region

in c. Starch grains, which are birefringent, appear as four white quadrantsseparated by a black cross. e, Longitudinal section of perisperm in post-fertilization seed with three-celled embryo and minimally developedendosperm. f, Higher magnification from boxed region in e of embryosurrounded by a small endosperm tissue that lacks significant storagereserves. The perisperm below the endosperm contains large amounts ofstarch (bright circular structures). Scale bars, 10mm. em, embryo; es,endosperm; fg, female gametophyte; ps, perisperm.

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Although endosperm is initiated in H. inconspicua (Fig. 3), peri-sperm, a diploid tissue derived from the maternal sporophyte, isthe major embryo-nourishing constituent within the seed5,15,18, as ischaracteristic of all members of the Nymphaeales2,19,20. The sexuallyformed endosperm ultimately occupies a very small portion of thematuring seed (Fig. 3) and does not play a significant role in thenourishment of the embryo.

Remarkably, the embryo-nourishing tissue within the ovule/seedin Hydatella begins to acquire significant carbon resources (starch)from the maternal plant before fertilization. At the two-nucleate stageof pre-fertilization female gametophyte development, the perispermcontains reserves of starch (Fig. 3). At the four-nucleate, four-celledstage of female gametophyte development, just before fertilization,cells of the perisperm are densely packed with starch grains. Afterfertilization (Supplementary Data) the seed continues to develop andthe volume of the perisperm increases (Fig. 3). Overall seed sizeincreases from approximately 450 mm in length by 235 mm in widthat the time of fertilization to 620 mm in length by 420 mm in width atmaturity. Thus in Hydatella a significant portion of the maternalcommitment of embryo-nourishing carbon reserves to ovules/seedsoccurs before fertilization.

Angiosperms (with the exception of Hydatellaceae) differ fromextant gymnosperms (conifers, Ginkgo, cycads, Gnetales) in thatmaternal commitment of embryo-nourishing resources to seeds onlyoccurs after fertilization21,22. This ontogenetic delay in seed provi-sioning (compared with ancestral non-flowering seed plants) haslong been viewed as an evolved and adaptive mechanism to allowthe maternal plant to allocate limited resources efficiently onlyto those seeds that have been successfully fertilized. As such, post-fertilization maternal resource allocation has been hypothesized to bea key innovation associated with the origin and subsequent radiationof flowering plants22.

The exceptional case of pre-fertilization maternal provisioningof a seed in Hydatella may well be an apomorphic feature ofHydatellaceae alone. If so, Hydatellaceae stands as an essentiallyunique angiosperm clade that has reverted to allocating resourcesto an ovule/seed before the initiation of an embryo (albeit to theperisperm and not to the female gametophyte, as in gymnosperms).Because most taxa of flowering plants use a sexually formedendosperm to nourish an embryo, pre-fertilization allocation ofembryo-nourishing reserves to the seed is ontogenetically precluded.However, members of a large number of disparate clades of angio-sperms, including many basal lineages (Nymphaeaceae, Cabombaceae,Trimeniaceae, Acoraceae, Ceratophyllaceae, Saururaceae, Piperaceae,Hydnoraceae), form an embryo-nourishing perisperm23–28. None ofthese diverse perisperm-forming lineages (with the possible exceptionof Acorus) allocates significant carbon resources to the ovule/seedbefore fertilization23–29. Importantly, if pre-fertilization provisioningof embryo-nourishing reserves should prove to be an apomorphy ofHydatellaceae, it is more strong proof that the earliest phases offlowering plant evolution were marked by a tremendous diversifica-tion of reproductive features.

Alternatively, pre-fertilization maternal resource allocation toovules/seeds, and specifically to a maternally derived perisperm, inHydatella could represent a plesiomorphic and transitional con-dition associated with the origin of flowering plants. If so, the ‘under-developed’ endosperm of Hydatellaceae and Nymphaeales is notreduced, but rather represents an intermediate condition (betweengymnosperms and other angiosperms) in which the endosperm hasnot yet achieved its fully fledged role as the primary source of nutri-ents for the developing embryo. It is important to note that Amborellaand most members of the Austrobaileyales contain a well-developedendosperm20 and do not form a perisperm (Trimenia being thereported exception27). Nevertheless, the prospect that early angios-perms might have used both a perisperm and an endosperm to nour-ish the embryo within a seed (as previously hypothesized24,30), andthat the maternal plant allocated reserves to this perisperm before

fertilization, is thoroughly congruent with the data derived fromHydatella and its new-found phylogenetic position.

Charles Darwin was among the first to recognize the immensechasm between gymnosperms and angiosperms with respect to theirbiological characteristics (letters to Oswald Heer in 1875 and toJoseph Hooker in 1879 and 1882). Since then, inferring the vegeta-tive, floral and reproductive features that defined the first angios-perms, as well as their evolutionary (transformational) links to agymnospermous ancestor, has proved to be fraught with difficulties.Indeed, the past five years have witnessed the near-global collapse of acentury-old set of paradigms concerning the embryological featuresof the earliest angiosperms3,13,17,20. With the present finding thatmaternal plants of Hydatella provision ovules/seeds with embryo-nourishing reserves before fertilization, yet another long-standinghypothesis for a presumed angiosperm-defining biological featureappears poised to be overturned.

METHODS SUMMARY

Hydatella inconspicua was collected on 6 December 2006 at Kai Iwi Lake,

Northland, New Zealand, by P. Champion, New Zealand National Institute of

Water and Atmospheric Research.

Plants were chemically fixed in 4% glutaraldehyde, washed in phosphate

buffer and stored in water. Specimens were dehydrated through an ethanol

series, then infiltrated and embedded in glycol methacrylate (JB-4 embedding

kit, Electron Microscopy Sciences). Embedded flowers were serially sectioned

into 4-mm thick ribbons. Sectioned flowers were stained with 0.1% toluidine blue

and examined under brightfield and cross-polarization conditions. Digital

imaging was on a Zeiss Axiocam digital camera using brightfield and cross

polarization optics. Images were processed with Adobe Photoshop CS2. Image

manipulations were restricted to operations applied to the entire image, except

as noted in specific figure legends.

Received 19 November 2007; accepted 15 January 2008.Published online 19 March 2008.

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Supplementary Information is linked to the online version of the paper atwww.nature.com/nature.

Acknowledgements I thank: P. Champion and A. Drinnan for collecting plantmaterials; S. Holloway for histological work; S. Renner for translation of theembryological studies of U. Hamaan; and P. Diggle, L. Hufford, J. Williams andR. Robichaux for feedback on this manuscript. This work was supported by aNational Science Foundation Research Grant.

Author Information Reprints and permissions information is available atwww.nature.com/reprints. Correspondence and requests for materials should beaddressed to W.E.F. (e-mail: [email protected]).

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