Vol. 269, No. 15 The Journal of April 15, 1994

Biological Chemistry Copyright 0 1994 by the American Society for Biochemistry and Molecular Biology, Inc.

428 East Preston St., Baltimore, MD 21202 U.S.A.

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CONTENTS*

MINIREVIEW Peptides containing a D-amino acid from frogs and molluscs. Giinther Kreil

COMMUNICATIONS Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis. Gabriele Fischer uon Mollurd, Bernd Stahl, Andrei Khokh- lutcheu, Thomas C. Sridhof, and Reinhard Jahn

Identification of G I U ~ ' ~ as the active-site nucleophile in human glucocerebrosidase by use of electrospray tan- dem mass spectrometry. Shichang Miao, John D. McCarter, Marie E. Grace, Gregory A. Grabowski, Ruedi Aebersold, and Stephen G. Withers

&cell glucose signaling. lain D. Dukes, Margaret S. Mc- Dependence on NADH produced during glycolysis for

Intyre, Robert J . Mertz, Louis H. Philipson, Michael W. Roe, Ben Spencer, and Jennings F. Worley III

of the cystic fibrosis chloride channel. Valentin K. Grib- The substituted benzimidazolone NS004 is an opener

h//, Guy Champigny, Pascal Barbry, Steven I. Dworetzky, Nicholos A. Meanwell, and Michel Lazdunski

Chemical characterization of AB 17-42 peptide, a com- ponent of diffuse amyloid deposits of Alzheimer disease. Eric Gowing, Alex E. Roher, Amina S. Woods, Robert J . Cotter, Michael Chaney, Sheila P. Little, and Melvyn J . Ball

ARTICLES Oncostatin M binds directly to gp130 and behaves as interleukin-6 antagonist on a cell line expressing gp130 but lacking functional oncostatin M receptors. Elisabetta Sporeno, Giacomo Paonessa, Anna Laura Salvati, Rita Graziani, Paolo Delmastro, Gennaro Ciliberto, and Carlo Toniatti

A soluble exopolyphosphatase of Saccharomyces cere-

and Arthur Kornberg visiae. Purification and characterization. Helmut Wurst

Mechanism of modulation of rat liver fructose-2,6-bis- phosphatase by nucleoside triphosphates. Yong-Hwan Lee, David Okar, Kai Lin, and Simon J. Pilkis

Defective asialoglycoprotein receptor endocytosis me- diated by tyrosine kinase inhibitors. Requirement for

J . Fallon, Maria Danaher, Robert L. Saylors, and Amit Saxena a tyrosine in the receptor internalization signal. Robert

Regulation of phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae by CDP-diacylglyc- erol. Joseph T. Nickels, Jr., Rosa J . Buxeda, and George M. Carman

Nonhomogeneous labeling of liver extra-mitochondrial acetyl-coA. Implications for the probing of lipogenic acetyl-coA via drug acetylation and for the production of acetate. by the liver. Ying Zhnng, Kamlesh C. Agarwal, Michel Beylot, Maxim V. Solouieu, France David, Mitchell W. Reider, Vernon E. Anderson, Kou-Yi Tserng, and Henri Brunengraber

D-type cyclin-binding regions of proliferating cell nu- clear antigen. Shuhei Matsuoka, Masamitsu Yamuguchi, and Akio Matsukage

Sequencing and characterization of the ntp gene cluster for vacuolar-type Na+-translocating ATPase of Enter- ococcus hirae. Kazuma Takase, Shizuko Kakinuma, Ichiro Yamato, Kiyoshi Konishi, Kazuei Igarashi, and Yoshimi Kakinuma

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Isothermal titration calorimetric studies of Saccharo- myces cereuisiae myristoyl-CoA:protein N-myristoyl- transferase. Determinants of binding energy and cata- lytic discrimination among acyl-CoA and peptide li-

A. Mc Wherter, and Jeffrey I. Gordon gands. Rajiv S. Bhutnagar, Emily Jackson-Machelski, Charles

Mutagenic studies of the interaction between the aspar- tate receptor and methyltransferase from Escherichia coli. Michael J . Shapiro and Daniel E. Koshlund, Jr.

Reduction of disulfide bonds in sarcoplasmic reticulum CaZ+-ATPase by dithiothreitol causes inhibition of phosphoenzyme isomerization in catalytic cycle. This reduction requires binding of both purine nucleotide and CaZ+ to enzyme. Takoshi Daiho and Tohru Kanazawa

Structure, genomic organization, and expression of the human interleukin-8 receptor B gene. Hans Sprenger, Andrew R. Lloyd, Laura L. Lautens, Tom I. Bonner, and David J. Kelvin

The complete primary structure for a novel laminin chain, the laminin B l k chain. Donald R. Gerecke, D. Wolfe Wagman, Marie-France Champliaud, and Robert E. Burgeson

A reverse gyrase with an unusual structure. A type I DNA topoisomerase from the hyperthermophile Meth- anopyrue kandleri is a two-subunit protein. Sergei A.

A. Lake, and Alexei I. Slesarev Kozyavkin, Regis Krah, Martin GeUert, Karl 0. Stetter, James

Phospholipids of Rhizobium contain nodE-determined

E. Thomas-Oates, John Glushka, Herman P. Spaink, and Ben highly unsaturated fatty acid moieties. Otto Geiger, Jane

J . J . Lugtenberg

Isolation and sequencing of insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Si- mon). Gary B. Quistad and Wayne S. Skinner

ulin. Yunje Cho, Carl A. Batt, and Lindsay Sawyer Probing the retinol-binding site of bovine &lactoglob-

108 Mutational analysis of the metal sites in an LIM do- main. James W. Michelsen, Andrew K. Sewell, Heather A. Louis, Jay I. Olsen, Darrell R. Davis, Dennis R. Winge, and Mary C. Beckerle

114 &Elimination of phosphate from reaction intermedi-

carboxylaseloxygenase. Frank W. Larimer, Mark R. Har- ates by site-directed mutants of ribulose-bisphosphate

p e l , and Fred C. Hartman

121 Recombinant replication protein A: expression, com- plex formation, and functional characterization. Leigh A. Henricksen, Christopher B. Umbricht, and Marc S. Wold

133 aV integrins mediate the rise in intracellular calcium in endothelial cells on fibronectin even though they play a minor role in adhesion. Martin Alexander Schwartz and Karl Denninghoff

138 Glycosphingolipid receptor function is modified by fatty acid content. Verotoxin 1 and verotoxin 2c pref- erentially recognize different globotriaosyl ceramide fatty acid homologues. Arash Kinrash, Beth Boyd, and Clifford A. Lingwood

11147 Measurements of ATP binding on the large cytoplasmic

expressed in Escherichia coli. Marie-Jo Moutin, Martine loop of the sarcoplasmic reticulum Ca2+-ATPase over-

Cuillel, Catherine Rapin, Roger Miras, Marielle Anger, Anne-Marie Lompre, and Yves Dupont

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Characterization of the protein-protein interactions determining the heat shock protein (hspOO*hsp70. hep66) heterocomplex. Michael J . Czar, Janet K.

M. Zacharek, Karen L. teach, Martin R. Deibel, Jr., and Owens-Grillo, Kurt D. Dittmar, Kevin A. Hutchison, Anthony

WiUiam B. Pratt

Lipooligosaccharide biosynthesis in pathogenic Neis- seria. Cloning, identification, and characterization of the phosphoglucomutase gene. Daogua Zhou, Dauid S. Stephens, Bradford W. Gibson, Jeffrey J. Engstrom, Carl F. McAUister, Frank K. N. Lee, and Michael A. Apicella

Mutational analysis of the substrate binding pockets of the Rous sarcoma virus and human immunodeficiency virus-1 proteases. Craig E. Cameron, Todd W. Ridky, Sergey

Alexander Wlodawer, Haim Burstein, Diane Bizub-Bender, Shulenin, Jonathan Leis, Irene T. Weber, Terry Copeland,

and Anna Marie Skalka

Functional expression and site-directed mutagenesis of a synthetic gene for a-bungarotosin. Julie A. Rosenthal, Sigmu+ H. Hsu, Dinesh Schneider, Lisa N. Gentile, Norma J. Messrer, Charles A. Vaslet, and Edward Hawrot

A peptide encoding the c-Jun 6 domain inhibits the activity of a c-Jun amino-terminal protein kinase. Vic- tor Adler, Tino Unlqp, and Andrew S. Kraft

Regulation of carboxypeptidase E. Effect of Caz+ on enzyme activity and stability. Srinivas R. Nalamachu, Lixin Song, and Lloyd D. Fricker

Identical mutations at corresponding positions in two homologous proteins with nonidentical effects. A. Joakim Bjorkman, R. Alan Binnie, L. Brent Cole, Huide Zhang, Mark A. Hermodson, and Sherry L. Mowbray

substrates and activation by NADPH. Kasim A. Mookh- Yeast squalene synthase. A mechanism for addition of

tiar, Stephen S. Kalinowski, Donglu Zhang, and C. Dale Poulter

Liver heparan sulfate structure. A novel molecular de- sign. Malcolm Lyon, Jon A. Deakin, and John T. Gallagher

Interaction of hepatocyte growth factor with heparan sulfate. Elucidation of the major heparan sulfate struc- tural determinants. Malcolm Lyon, Jon A. Deakin, Kensaku Mizuno, Toshikazu Nakamura, and John T. Gallagher

External ATP and its analogs activate the cystic fibro- sis transmembrane conductance regulator by a cyclic

Adriana G. Prat, I g m M L. Reisin, Liliana B. Ercole, Edward AMP-independent mechanism. Horacio F. Cantiello,

A. Ausiello H. Abraham, Jane F. Amara, Richard J. Gregory, and Dennis

Zoran RadE, Rosarw Duran, Daniel C. Vellom, Ying Li, Carlos Site of fasciculin interaction with acetylcholinesterase.

Cervenansky, and Palmer Taylor

Kinetic mechanism of phosphatelphosphate and phos- phate/OH- antiports catalyzed by reconstituted phos- phate carrier from beef heart mitochondria. Reiner Stap- pen and Reinhard Kramer

Thimerosal potentiates Ca*+ release mediated by both the inositol 1.4,S-trisphosphate and the ryanodine receptors in sea urchin eggs. Implications for mecha- nistic studies on Ca" signaling. Yuji Tanaka and Armen H. Tashjian, Jr.

Purification and characterization of isoquinoline l-ox- idoreductase from Pseudomonas dirninuta 7, a novel molybdenum-containing hydroxylase. Martin Lehmann, Barbara Tshiswka, Susanne Fetzner, Petra Roger, and Franz Lingens

nucleotide binding in the noncatalytic sites of Esche- Tryptophan fluorescence provides a direct probe of

richia coli F1-ATPase. Joachim Weber, Susan Wilke-Mounts, Ernst Grell, and Alan E. Senior

an arginine aminopeptidase of high efficiency and spec- The bifunctional enzyme leukotriene-A, hydrolase is

ificity. Lars Orning, J. K. Gierse, and F. A. Fitzpatnck

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Dissociation as a result of phosphorylation of an aggre-

Kato, Kaori Hasegawa, Sachiyo Goto, and Yutaka Inaguma gated form of the small stress protein, hsp27. Kanefusa

Critical nucleotides in the interaction of a LysR-type regulator with its target promoter region. catBC pro- moter activation by CatR. Matthew R. Parsek, Rick w. Ye, Pattle Pun, and A. M. Chakrabarty

A family of proteins that stabilize the RanPC4 GTPase

Adrienne L. Beddow, and Ian G. Macara in its GTP-bound conformation. Karen M. Lounsbury,

Interleukin la mediates collagenase synthesis stimu- lated by phorbol 12-myristate 13-acetate. M. Elizabeth Fini, Katherine J . Strissel, Marie T. Girard, Judith West Mays, and William B. Rinehart

The interferon-induced 67-kDa guanylate-binding protein (hGBP1) is a GTPase that converts GTP to GMP. Martin Schwemmle and Peter Staeheli

Primary structure analysis and lamin B and DNA bind- ing of human LBR. an integral protein of the nuclear envelope inner membrane. Qian Ye and Howard J . Worman

Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner mem- brane. Ekkehard Schuler, Feng Lin, and Howard J. Worman

Molecular cloning and biological activity of a novel developmentally regulated gene encoding a protein with 8-transducin-like structure. Sharad Kumar, Tomoko Matsuzaki, Yoko Yoshida, and Makoto Noda

Photoidentification of mannosyltransferases of doli-

characterization of GDP-Man:Ma#?l- 4GlcNAcfll+ chol cycle in the mammary gland. Purification and

Mudgapalli, Samir K. Roy, Eric H. Holmes, and Inder K. VtJay 4GlcNAc-P-P-dolichol mannosyltransferase. As+k

phoephatidylcholine biosynthesis and up-regulation of Free cholesterol loading of macrophages stimulates

CTP: phosphoeholine cytidylyltransferase. Yoshimune Shiratori, Anselm K. Okwu, and Ira Tabas

identified mutation in the catalytic loop of the tyrosine Substitution of glutamine for arginine 1131. A newly

kinase domain of the human insulin receptor. Miyako Kishimoto, Mitsuru Hashiramoto, Kazuyoshi Yonezawa, Kozui Shii, Tsutomu Kazumi, and Masato Kasuga

Reconstitution of recombinant 33-kDa subunit of the clathrin-coated vesicle H+-ATPase. Sheng-Bin Peng,

stone Ying Zhang, Sue Jean Tsai, Xiao-Song Xie, and Dennis K.

A hammerhead ribozyme inhibits the proliferation of an RNA coliphage SP in Escherichia coli. Yoshw Inoku- chi, Noriko Y u y a m , Akikazu Hirashimu, Satoshi Nishikawa, Jun Ohkawa, and Kazunari Taira

The covalent eukaryotic topoisomerase I-DNA inter-

holysis. Kent Christiansen, Birgitta R. Knudsen, and Ole mediate catalyzes pH-dependent hydrolysis and alco-

Westergaard

Characterization of a stable form of human meizo- thrombin derived from recombinant prothrombin

K. Steuens, Laszlo BaJzar, Daw? K. Banfield, Michael E. (RlSSA, R271A, and R284A). Heline C. F. C&e, Willem

Nesherm, and Ross T. A. MacGrllwray

Characterization of type I procollagen N-proteinase from fetal bovine tendon and skin. Purification of the 600-kllodalton form of the enzyme from bovine tendon.

guerite-Marie Boutillon, Michel wan der Rest, JoAnn Mc- Yoshio Hojima, Matthius M. Morgelin, Jurgen Engel, Mar-

Kenzie, Gi-Chung Chen, Nasrin Rafi, Anne M. Romanic, and Darwin J . Prockop

The di- and tripeptide transport protein of Lactococc? lactis. A new type of bacterial peptide transporter. AnJa Hagting, Edmund R. S. Kunji, Kees J. Leenhouts, Bert Pool- man, and Wil N. Konings

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Pennsylvania, and Canada add applicable taxes, unless a tax-exempt certi;ficate number -is shown.) The GST'number for Canadian subscribers is

address changes to THE JOURNAL OF BIOLOGICAL CHEMISTRY at 428 East Preston Street, Baltimore, MD 21202, U.S.A. 130369721. Second class postage paid at Baltimore, MD 21202, U.S.A. and at additional mailing offices. Printed in the U.S.A. POSTMASTER Send

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A partial structural repeat forms the heterodimer self- association site of all B-spectrins. Scott P. Kennedy, Scott A. Weed, Bernard G. Forget, and Jon S. Morrow

ulin on B cells provides an inhibitory signal that closes Cross-linking of Fc-y receptor to surface immunoglob-

the plasma membrane calcium channel. Michael L. Diegel, Bruce M. Rankin, Joseph B. Bolen, Patrice M. Dubois, and Peter A. Kiener

Alteration of lipid profiles in plasma of transgenic mice expressing human lipoprotein lipase. Ming-Sun Liu, Frank R. Jirik, Renee C. LeBoeuf, Howard Henderson, Law-

Forsytk, Hanfang Zhang, Elizabeth Kirk, John D. Brunzell, rence W. Castellani, Aldons J . Lusis, Yuanhong Ma, Ian J.

and M ~ h a e l R. Hayden S p l is a critical factor for the monocytic specific expression of human CD14. Dong-Er Zhang, Christopher J . Hetherington, Shencao Tan, Suzan E. Dziennis, Davld A. Gonzalez, Hui-Min Chen, and Daniel G. Tenen

Biochemical characterization of valosin-containing protein, a protein tyrosine kinase substrate in hema- topoietic cells. Mark Egerton and Lawrence E. Samekon

Mammalian AMP-activated protein kinase is homolo- gous to yeast and plant protein kinasea involved in the regulation of carbon metabolism. David Curling, Kripamoy Aguan, Angela Woods, Adrie J. M. Verhoeuen, Raj K. Beri,

and James Scott Caroline H. Brennan, Chris Sidebottom, Matthew D. Dauison,

factor-1 (LGF-1) receptor having the carboxyl-terminal Signal transduction by a chimeric insulin-like growth

Mothe, Aline Kowakki-Chauuel, Jean-Philippe Breittmyer, domain of the insulin receptor. Sophie Tartare, Isabelle

Robert Ballotti, and Emmanuel Van Obberghen

Disulfide mutants of the binding domain of the rat low

N. Baldwin and Eric M. Shooter affinity nerve growth factor receptor ( ~ 7 6 ~ ' ~ ) . Anne

matrix vesicles by binding to type I1 and X collagens. Stimulation of calcification of growth plate cartilage

Thorsten Kirsch and Roy E. Wuthier

Regulation of immunoreactive insulin-like growth fac- tor binding protein-6 in normal and transformed hu- man fibroblasts. Janet L. Martin, Jennifer A. Coverley, and Robert C. Baxter

Catalysis by Syrian hamster 3-hydroxy-3-methylglu- taryl-coenzyme A reductase. Proposed roles of histidine

pong and Victor W. Rodwell 866, glutamate 668, and aspartate 766. Kenneth Frim-

Yolk protein factor 1 is a Drosophila homolog of Ku, the DNA-binding subunit of a DNA-dependent protein kinase from humans. Dough B . Jacoby and Pieter C. Wensink

Molecular characterization of light chain 3. A micro- tubule binding subunit of MAPlA and MAPlB. Suzanne S. Mann and James A. Hammarback Characterization of a 60-kilodalton substrate of the insulin receptor kinase. Yoichi Hosomi, Kozui Shii, Wataru Ogawa, Hiroshi Matsuba, Masaki Yoshida, Yumi O M , Koichi Yokono, Masato Kasuga, Shigeaki Baba, and Rlchard A. Roth

Xylosyl transfer to an endogenous renal acceptor. Characteristics of the reaction and properties of the product. Elks Meezan, Sandya Ananth, Stephen Manzella, Patrick Campbell, Stuart Siegal, Dennis J . Pillion, and k n n a r t Roden Xylosyl transfer to an endogenous renal acceptor. Pu- rification of the transferase and the acceptor and their identification as glycogenin. Lennart Roden, Sandya An- anth, Patrick Campbell, Stephen Manzella, and Elks Meezan Analysis of androgen receptor-DNA interactions with receptor proteins produced in insect cells. Pekka J . Kal- lio, Jorma J . Paluimo, Merja Mehto, and Olli A. Janne

both low and high affinity glucose-stimulated insulin GLUT-2 gene transfer into insulinoma cells confers

release. Relationship to glucokinase activity. Sarah Fer- ber, Hector BeltrandelRio, John H. Johnson, Richard J . Noel,

Hughes, and Christopher B. Newgard Laura E. Cassidy, Samuel Clark, Thomas C. Becker, Steven D.

Human cathepsin S: chromosomal localization, gene structure, and tissue distribution. Guo-Ping Shi, Andrew C. Webb, Karen E. Foster, Joan H. M. Knoll, Cynthla A. Lemere, John S. Munger, and Harold A. Chapman

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Functional role of a cytoplasmic aromatic amino acid in muscarinic receptor-mediated activation of phospho- lipase C. Klaus Bliiml, Ernst Mutschler, and Jurgen Wess

Incorporation of adenovirus into a ligand-based DNA carrier system results in retention of original receptor specificity and enhances targeted gene expression. George Y. Wu, Peili Zhan, Lillian L. Sze, Arielle R. Rosenberg, and Catherine H. W u

Type I phosphatidylinositol 4-phosphate &kinase iso-

Glenn H. Jenkins, Philip L. Fisette, and Richard A. Anderson forms are specifically stimulated by phosphatidic acid.

binding protein of N-glycans presented on neoglyco- Differential recognition by conglutinin and mannan-

lipida and glycoproteins with special reference to com- plement glycoprotein C3 and ribonuclease B. Dolores

Richard A. Harrison, and R. Wendy Loveless Solis, Ten Feizi, Chun-Ting Yuen, Alexander M. Lawson,

DNA distortion and nucleation of local DNA unwinding within sigma-64 (p) holoenzyme closed promoter com- plexes. Lorna Morris, Wendy Cannon, Felix Clauerie-Martin, Sara Austin, and Martin Buck

Hepatic lipase induces the formation of pre-& high density lipoprotein (HDL) from triacylglycerol-nch HDLs. A study comparing liver perfusion to in vitro incubation with lipases. Alain Barrans, Xauier CoUet, Ron- ald Barburas, Beatrice Jaspard, Jeanine Manent, Claude Vieu, Hugues Chap, and Bertrand Perret

porter into an outward facing conformation. Hiroyuki Substitution of tyrosine 293 of GLUT1 locks the trans-

Mori, Mitsuru Hashiramoto, Auril E. Clark, Jing Yang, Akihiro Muraoka, Yoshikazu Tamori, Masato Kasuga, and Geoffrey D. H o l m n

Self-assembly into fibrils of collagen I1 by enzymic cleavage of recombinant procollagen 11. Lag period, critical concentration, and morphology of fibrils differ from collagen I. Andrzej Fertala, Aleksander L. Sieron, Yoshw Hoj im, Arum Ganguly, and Darwin J. Prockop

Analysis of binding to mutated form of protein kinase Zinc finger domains and phorbol ester pharmacophore.

Marcelo G. Kazanietz, Xose R. Bustelo, Mariano Barbacid, C { and the vav and c-raf proto-oncogene products.

Walter Kolch, Harald Mischak, Garry Wong, George R. Pettit, Jay D. Bruns, and Peter M. Blumberg

Control of glycogen synthase and phosphorylase by amylin in rat skeletal muscle. Hormonal effects on the phosphorylation of phosphorylase and on the distribu- tion of phosphate in the synthase subunit. John C. Law- rence, Jr. and Ji-nan Zhang

A role for the C2 domain of factor VI11 in binding to

K. J . Rajahkshmi, and Dorothea Scandella von Willebrand factor. Evgueni L. Saenko, Midori S h i m ,

Conformation of Cas+-ATPase in two crystal forms. Effects of Ca", thapsigargin, adenosine S'-@,y-meth- ylene)triphosphate), and chromium(II1)-ATP on crys- tallization. David L. Stokes and Jean-Jacques Lucapkre

Self-assembly of collagen I from a proband homozygous for a mutation that substituted serine for glycine at position 661 in the a2(I) chain. Possible relationship between the effects of mutations on critical concentra- tion and the severity of the phenotype. Anne M. Romnic, Loretta D. Spotila, Eijiro Adachi, Jurgen Engel, Yoshw Hojim, and Darwin J . Prockop

Fibroblast growth factor receptor (FGFR) 3. Alterna- tive splicing in immunoglobulin-like domain III creates a receptor highly specific for acidic FGFFGF-1. Arasu T. Chellaiah, D o d G. McEwen, Sabine Werner, Jingsong Xu, and David M. Ornitz

Angiotensin I1 inhibits cytokine-stimulated inducible nitric oxide synthase expression in vascular smooth muscle cells. Ichiro Nakayam, Yasuhiro Kawahara, Teru- taka Tsuda, Masanori Okuda, and Mitsuhiro Yokoyam

Class I histocompatibility molecule association with phosphorylated calnexin. Implications for rates of in- tracellular transport. G. George Capps and Martha C. Zuriiga

11640 Purification and characterization of 240-kDa cGMP- dependent protein kinase substrate of vascular smooth muscle. Close resemblance to inositol 1,4,S-trisphos- phate receptor. Takaki Koga, Yutaka Yoshida, Ji-Qun Cui, Md Omedul Islam, and Shoichi I m i

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11648 Ciliary neurotrophic factorfleukemia inhibitory factor/ interleukin 6/oncostatin M family of cytokines induces tyrosine phosphorylation of a common met of proteins overlapping thome induced by other cytokines and growth factors. Teri G. Boulton, Ned Stahl, and George D. Yancopoulos

11656 Negative modulator of the rat Dz dopamine receptor ene. Takashi Minowa, Mari T . Minowa, and M. Maral ouradiun

11663 Characterization of the genomic structure, chromo- somal location, romoter, and developmental expres- sion of the a- login transcription factor CP2. Steven L. Swendemnn, C%arles Spielholz, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, and Michnel Sheffery

I 4

ADDITIONS AND CORRECTIONS 11672 Molecular characterization and expression of a gene

encoding a Staphylococcus awe- collagen adhesin.

son, Bengt Gus, Lech M. Switalski, rzsttnu Wtberg, Martin Vol. 267 (1992) 4766-4772. J0seih.M. Pattl, Hans Jons-

Lindberg, and Magnus H66k

11672 The release of endothelium-derived relaxing factor via az-adrenergic receptor activation is specifically me-

Liao and Charles J. Homey diated by Gw. Vol. 268 (1993) 19628-19533. James K.

11673 Overexpression of human lipoprotein lipase in trans- genic mice. Resistance to diet-induced hypertriglycer- idemia and hypercholesterolemia. Vol. 268 (1993) 17924-17929. Masaka Shimada, Hitoshi Shimno, Taka- nuri Gotoda, Koji Yamnmoto, Masako Kawamura, Toshimori Znabq Yoshw Yazaki, and Nobuhiro Yamada

11673 Long range propagation of conformational changes in

Du, Minyi Gu, John W. Weisel, Chandrasekaran Nagaswami, integrin 4 s . Vol. 268 (1993) 23087-23092. Xiooping

Joel S. Bennett, Ron Bowditch, and Mark H. Ginsberg

11674 Thyroid hormone receptor modulates the expression of the rabbit cardiac sarco(endo)plasmic reticulum Caz*- ATPase gene. Vol. 269 (1994) 1460-1467. Angel Zar- ain-Herzberg, John Marques, Drew Sukovich, and Muthu Periasamy

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The Journal of Biological Chemistry

CONTENTS Arranged by Subject Categories

MINIREVIEW Peptides containing a D-amino acid from frogs and molluscs. Giinther Kreil

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

Phospholipids of Rhizobium contain nodE-determined

E. Thomas-Oates, John Glushka, Herman P. Spaink, and Ben highly unsaturated fatty acid moieties. Otto Geiger, Jane

J . J . Lugtenberg

Glycosphingolipid receptor function is modified by fatty acid content. Verotoxin 1 and verotoxin 2c pref- erentially recognize different globotriaosyl ceramide fatty acid homologues. Arash Kiarash, Beth Boyd, and Clifford A. Lingwood

Lipooligosaccharide biosynthesis in pathogenic Neis- seria. Cloning, identification, and characterization of the phosphoglucomutase gene. Daoguo Zhou, David S.

McAllister, Frank K. N. Lee, and Michael A. Apicella Stephens, Bradford W. Gibson, Jeffrey J . Engstrom, Carl F.

sign. Malcolm Lyon, Jon A. Deakin, and John T. Gallogher Liver heparan sulfate structure. A novel molecular de-

Photoidentification of mannosyltransferases of doli- chol cycle in the mammary gland. Purification and characterization of GDP-Man:Madld 4GlcNAcj31+ 4GlcNAc-P-P-dolichol mannosyltransferase. Ashok Mudgapalli, Samir K. Roy, Eric H. Holmes, and Inder K. Vijay

Free cholesterol loading of macrophages stimulates phosphatidylcholine biosynthesis and up-regulation of CTP: phosphocholine cytidylyltransferase. Yoshimune Shiratori, Anselm K. Okwu, and Ira Tabas

Xylosyl transfer to an endogenous renal acceptor.

product. Elks Meezan, Sandya Anunth, Stephen Manzella, Characteristics of the reaction and properties of the

Patrick Campbell, Stuart Siegal, Dennis J . Pillion, and Lennurt Roden

Xylosyl transfer to an endogenous renal acceptor. Pu- rification of the transferase and the acceptor and their identification as glycogenin. Lennurt Roden, Sandya An- anth, Patrick Campbell, Stephen ManzeUa, and Elias Meezan

Differential recognition by conglutinin and mannan- binding protein of N-glycans presented on neoglyco- lipids and glycoproteins with special reference to com- Plement glycoprotein C3 and ribonuclease B. Dolores

Richard A. Harrison, and R. Wendy Loveless solis, Ten Feizi, Chun-Ting Yuen, Alexander M. Lawson,

Hepatic lipase induces the formation of pre& high density lipoprotein (HDL) from triacylglycerol-rich HDL.. A study comparing liver perfusion to in vitro incubation with lipases. Alnin Barrans, Xavier Collet, Ron-

Hugues Chap, and Bertrand Perret ald Barbaras, Beatrice Jaspard, Jeanine Manent, Claude Vieu,

Overexpression of human lipoprotein lipase in trans- genic mice. Resistance to diet-induced hypertriglycer- idemia and hypercholesterolemia. Vol. 268 (1993) 17924-17929. Masako Shimda, Hitoshi Shimano, Taka- nuri Got&, Koji Yamamoto, Masako Kawamura, Toshimori I d a , Yoshw Yazaki, and Nobuhiro Y a d a

CELL BIOLOGY AND METABOLISM Rab3C is a synaptic vesicle protein that dissociates

Gabriele Fischer uon Mallard, Bernd Stahl, Andrei Khokh- from synaptic vesicles after stimulation of exocytosis.

latchev, Thomas C. Sudhof, and Reinhard Jahn

Dependence on NADH produced during glycolysis for &cell glucose signaling. Iain D. Dukes, Margaret S. Mc- Intyre, Robert J . Mertz, Louis H. Philipson, Michael W. Roe, Ben Spencer, and Jennings F. Worley III

10991

11011

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11349

April 15, 1994

Oncostatin M binds directly to gp130 and behaves as interleukin-6 antagonist on a cell line expressing gp130 but lacking functional oncostatin M receptors. Elisabetta Sporeno, Giaeomo Paonessa, Anna Laura Salvati, Rita Graziani, Paolo Delmastro, Gennuro Ciliberto, and Carlo Toniatti

Defective asialoglycoprotein receptor endocytosis me- diated by tyrosine kinase inhibitors. Requirement for a tyrosine in the receptor internalization signal. Robert J. Fallon, Maria Danuher, Robert L. Saylors, and Amit Saxena

Nonhomogeneous labeling of liver extra-mitochondrial acetyl-coA. Implications for the probing of lipogenic acetyl-coA via drug acetylation and for the production

Michel Beylot, Maxim V. Soloviev, France David, Mitchell W. of acetate by the liver. Ying Zhang, Kamlesh C. Agarwal,

Reider, Vernon E. Anderson, Kou-Yi Tserng, and Henri Brunengraber

clear antigen. Shuhei Matsuoka, Masamitsu Yamaguchi, and D-type cyclin-binding regions of proliferating cell nu-

Akio Matsukage

a, integrins mediate the rise in intracellular calcium in endothelial cells on fibronectin even though they play

Karl Denninghoff a minor role in adhesion. Martin Alexander Schwartz and

Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70. hsp56) heterocomplex. Michuel J . Czar, Janet K.

M. Zachurek, Karen L. Leach, Martin R. Deibel, Jr., and Owens-Grillo, Kurt D. Dittmar, Kevin A. Hutchison, Anthony

William B. Pratt

Interaction of hepatocyte growth factor with heparan sulfate. Elucidation of the major heparan sulfate struc- tural determinants. Malcolm Lyon, Jon A. Deakin, Kensaku Mizuno, Toshikazu Nakamura, and John T. Gallogher

Thimerosal potentiates Caz+ release mediated by both the inositol 1,4,5-trisphosphate and the ryanodine receptors in sea urchin eggs. Implications for mecha- nistic studies on Caz+ signaling. Yuji Tanuka and Arrnen H. Tashjian, Jr.

Dissociation as a result of phosphorylation of an aggre- gated form of the small stress protein, hsp27. Kanefusa Kato, Kaori Hasegawa, Sachiyo Goto, and Yutaka Inoguma

A family of proteins that stabilize the Ran/TC4 GTPase in its GTP-bound conformation. Karen M. Lounsbuv, Adrienne L. Beddow, and Ian G. Macara

Interleukin la mediates collagenase synthesis stimu- lated by phorbol 12-myristate 13-acetate. M. Elizabeth Fini, Katherine J. Strissel, Marie T. Girard, Judith West Mays, and William B. Rinehart

protein (hGBP1) is a GTPase that converts GTP to The interferon-induced 67-kDa guanylate-binding

GMP. Martin Schwemmle and Peter Staeheli

Primary structure analysis and lamin B and DNA bind-

envelope inner membrane. Qian Ye and Howard J . ing of human LBR, an integral protein of the nuclear

Worman

Characterization of the human gene encoding LBR, an

brane. Ekkehard Schuler, Feng Lin, and Howard J. Worman integral protein of the nuclear envelope inner mem-

Molecular cloning and biological activity of a novel developmentally regulated gene encoding a protein

Matsuzaki, Yoko Yoshida, and Makoto Noda with j3-transducin-like structure. Shard Kumar, Tomoko

Substitution of glutamine for arginine 1131. A newly identified mutation in the catalytic loop of the tyrosine

Kishimoto, Mitsuru Hashiramoto, Kazuyoshi Yonezawa, Kozui kinase domain of the human insulin receptor. Miyako

Shii, Tsutomu Kazumi, and Masato Kasuga

Indicates a Communication viii

ix

1409 Cross-linking of Fcy receptor to surface immunoglob- ulin on B cells provides an inhibitory signal that closes

Bruce M, Rankin, Joseph B. Bolen, Patrice M. Dubois, and the plasma membrane calcium channel. Michuel L. Diegel,

Peter A. Kiener

,1435 Biochemical characterization of valosin-containing protein, a protein tyrosine kinase substrate in hema- topoietic cells. Mark Egerton and Lawrence E. Samelson

,1442 Mammalian AMP-activated protein kinase is homolo- gous to yeast and plant protein kinases involved in the regulation of carbon metabolism. David Carling, Kripamoy Aguan, Angela Woods, Adrie J. M. Verhoeven, Raj K. Beri, Caroline H. Brennnn, Chris Sidebottom, Matthew D. Dauison, and James Scott

11449 Signal transduction by a chimeric insulin-like growth factor-1 (IGF-1) receptor having the carboxyl-terminal domain of the insulin receptor. Sophie Tartare, Isabelle

Robert Ballotti, and Emmanuel Van Obberghen Mothe, Aline Kowalski-Chuuvel, Jean-Philippe Breittmayer,

,1470 Regulation of immunoreactive insulin-like growth fac- tor binding protein-6 in normal and transformed hu-

Robert C. Baxter man fibroblasts. Janet L. Martin, Jennifer A. Coverley, and

,1492 Molecular characterization of light chain 3. A micro- tubule binding subunit of MAPlA and MAPlB. Suzanne S. Mann and James A. Hammarback

.1498 Characterization of a 60-kilodalton substrate of the insulin receptor kinase. Yoichi Hosomi, Kozui Shii, Wataru Ogawa, Hiroshi Matsuba, Mvak i Yoshida, Yumi O W a , Koichi Yokono, Masato Kasuga, Shgeakr Baba, and Rrchurd A. Roth

11523 GLUT-2 gene transfer into insulinoma cells confers both low and high affinity glucose-stimulated insulin release. Relationship to glucokinase activity. Sarah Fer- ber, Hector BeltrandelRio, John H. Johnson, Richurd J. Noel, Laura E. Cassidy, Samuel Clark, Thomas C. Becker, Steven D. Hughes, and Christopher B. Newgard

11537 Functional role of a cytoplasmic aromatic amino acid in muscarinic receptor-mediated activation of phospho- lipase C. Klaus Bluml, Ernst Mutschler, and Jurgen Wess

11595 Control of glycogen synthase and phosphorylase by amylin in rat skeletal muscle. Hormonal effects on the phosphorylation of phosphorylase and on the distribu- tion of phosphate in the synthase subunit. John C. Law- rence, Jr. and Ji-nun Zhung

11620 Fibroblast growth factor receptor (FGFR) 3. Alterna- tive splicing in immunoglobulin-like domain III creates a receptor highly specific for acidic FGFPGF-1. Arasu

Xu, and David M. Ornitz T. CheUaiah, Donald G. McEwen, Sabine Werner, Jingsong

11628 Angiotensin I1 inhibits cytokine-stimulated inducible nitric oxide synthase expression in vascular smooth muscle cells. Zchiro Nakayama, Yasuhiro Kawahara, Teru- taka Tsuda, Masanori Okudo, and Mitsuhiro Yokoyama

11634 Class I histocompatibility molecule association with phosphorylated calnexin. Implications for rates of in- tracellular transport. G. George Capps and Marthu C. Zuriiga

11640 Purification and characterization of 240-kDa cGMP- dependent protein kinase substrate of vascular smooth muscle. Close resemblance to inositol l,.i,S-trisphos-

Md Omedut Zshm, and Shoichi Imai phate receptor. Takuki Koga, Yutaka Yoshida, Ji-Qun Cai,

11648 Ciliary neurotrophic factorfleukemia inhibitory factor/ interleukin B/oncostatin M family of cytokines induces tyrosine phosphorylation of a common set of proteins overlapping those induced by other cytokines and growth factors. Teri G. Boulton, Neil Stahl, and George D. Yancopoulos

11672 The release of endothelium-derived relaxing factor via up-adrenergic receptor activation is specifically me- diated by Gm. Vol. 268 (1993) 19628-19533. James K. Liao and Churles J . Homcy

010975

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11114

11192

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11367

11381

11478

11037

11060

11147

ENZYMOLOGY

human glucocerebrosidase by use of electrospray tan- Identification of Glu*'O as the active-site nucleophile in

dem maas spectrometry. Shichang Miao, John D. McCarter, Marie E, Grace, Gregory A. Grabowski, Ruedi Aebersold, and Stephen G. Withers

A soluble exopolyphosphatase of Saccharomyces cere-

and Arthur Kornberg uisiae. Purification and characterization. Helmut Wurst

Mechanism of modulation of rat liver fructose-2.6-bis- phosphatase by nucleoside triphosphates. Yong-Hwan Lee, David Okur, Kai Lin, and Simon J . Pilkis

Regulation of phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae by CDP-diacylglyc- erol. Joseph T. Nickels, Jr., Rosa J . Bureda, and George M. Carman

Isothermal titration calorimetric studies of Saccharo-

transferase. Determinants of binding energy and cata- myces cerevisiae myristoyl-CoAprotein N-myristoyl-

lytic discrimination among acyl-CoA and peptide li-

A. McWherter, and Jeffrey I. Gordon gands. Rajiu S. Bhutnagar, Emily Jackson-Machelski, Charles

&Elimination of phosphate from reaction intermedi- ates by site-directed mutants of ribulose-bisphosphate

pe l , and Fred C. Hartman carboxylase/oxygenase. Frank W. hr imer , Mark R. Har-

Regulation of carboxypeptidase E. Effect of Cap' on enzyme activity and stability. Sriniuas R. Nalamachu, Lixin Song, and Lloyd D. Fricker

substrates and activation by NADPH. Kasim A. Mookh- Yeast squalene synthase. A mechanism for addition of

tiur, Stephen S. Kalimwski, DongluZhng, and C. Dale Poulter

Purification and characterization of isoquinoline l-ox- idoreductase from Pseudomonas diminuta 7, a novel molybdenum-containing hydroxylase. Martin Lehmann,

Lingens Barbara Tshiswku, Susanne Fetzner, Petm Rigger, and Franz

The bifunctional enzyme leukotriene-A, hydrolase is an arginine aminopeptidase of high efficiency and spec- ificity. Lars Orning, J. K. Gierse, and F. A. Fitzpatrick

The covalent eukaryotic topoisomerase I-DNA inter-

holysis. Kent Christiunsen, Birgitta R. Knudsen, and Ole mediate catalyzes pH-dependent hydrolysis and alco-

Westergaurd

Characterization of type I procollagen N-proteinase from fetal bovine tendon and skin. Purification of the 500-kilodalton form of the enzyme from bovine tendon. Yoshw H o j i w Matthias M. Margelin, Jurgen Engel, Mar- guerite-Marie Boutillon, Michel van der Rest, JoAnn Mc-

Darwin J. Prockop Kenzie, Gi-Chung Chen, Nasrin Rafi, Anne M. Romanic, and

Catalysis by Syrian hamster 3-hydroxy-3-methylglu- taryl-coenzyme A reductase. Proposed roles of histidine 866, glutamate 668, and aspartate 766. Kenneth Frim- pong and Victor W. Rodwell

MEMBRANES AND BIOENERGETICS Sequencing and characterization of the ntp gene cluster for vacuolar-type Na+-translocating ATPase of Enter- OCOCCUB hirue. Kazuma T a k e , S h u u k o Kakinuma, Ichiro Yamato, Kiyoshi Konishi, Kazuei Zgarashi, and Yoshimi Kakinuma

Reduction of disulfide bonds in sarcoplasmic reticulum Caa*-ATPase by dithiothreitol causes inhibition of phosphoenzyme isomerization in catalytic cycle. This reduction requires binding of both purine nucleotide and Cap+ to enzyme. T a k h i Daihn and Tohru Kamawa

Measurements of ATP binding on the large cytoplasmic loop of the sarcoplasmic reticulum Caa+-ATPase over- expressed in Escherichia coli. Marie-Jo Moutin, Martine CuiUel, Catherine Rapin, Roger Miras, MarieUe Anger, Anne-Marie Lompre, and Yues Dupont

Indicates a Communication

X

11224

11240

11261

11356

11391

11547

11578

External ATP and ita analogs activate the cystic fibro- sis transmembrane conductance regulator by a cyclic

Adriana G. Prat, Ignacw L. Reisin, Liliana B. Ercole, Edward AMP-independent mechanism. Horacw F. Cantiello,

H. Abraham, Jane F. Amara, Richard J . Gregory, and Dennis A. Ausiello

Kinetic mechanism of phosphate/phosphate and phos- phate/OH- antiports catalyzed by reconstituted phos-

pen and Reinhard Kramer phate carrier from beef heart mitochondria. Reiner Stap-

Tryptophan fluorescence provides a direct probe of nucleotide binding in the noncatalytic sites of Esche- richia coli FI-ATPase. Joachim Weber, Susan Wilke-Mounts, Ernst Grell, and Alan E. Senior

Reconstitution of recombinant 33-kDa subunit of the clathrin-coated vesicle H+-ATPase. Sheng-Bin Peng,

stone Ying Zhang, Sue Jean Tsai, Xino-Song Xie, and Dennis K.

lactis. A new type of bacterial peptide transporter. Anja The di- and tripeptide transport protein of Lactococcus

Hagting, Edmund R. S. Kunji, Kees J . Leenhouts, Bert Pool- man, and Wil N. Konings

Type I phosphatidylinositol 4-phosphate 5-kinase iso- forms are specifically stimulated by phosphatidic acid. Glenn H. Jenkins, Philip L. Fisette, and Richard A. Anderson

porter into an outward facing conformation. Hiroyuki Substitution of tyrosine 293 of GLUT1 locks the trans-

Mori, Mitsuru Hashiramoto, Avril E. Clark, Jing Yang, Akihiro Muraoka, Yoshikuzu Tamori, Masato Kasuga, and Geoffrey D. Holman

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

11065 Structure, genomic organization, and expression of the

Andrew R. Lloyd, Laura L. Luutens, Tom I. Bonner, and David human interleukin-8 receptor B gene. Hans Sprenger,

J . Keluin

11081 A reverse gyrase with an unusual structure. A type I

anopyrue kuder i is a two-subunit protein. Sergei A. DNA topoisomerase. from the hyperthermophile Meth-

Kozyaukin, Regis Krah, Martin Gellert, Karl 0. Stetter, James A. L a k e , and Alexei I. Slesarev

11121 Recombinant replication protein A expression, com- plex formation, and functional characterization. Leigh A. Henricksen, Christopher B. Umbricht, and Marc S. Wold

11170 Mutational analysis of the substrate binding pockets of the Rous sarcoma virus and human immunodeficiency virus- 1 proteases. Craig E. Cameron, Todd W. Ridky, Sergey Shulenin, Jonathan Leis, Irene T. Weber, Terry Copeland, Alexander Wlodawer, Haim Burstein, Diane Bizub-Bender, and Anna Marie Skolka

1279 Critical nucleotides in the interaction of a LysR-type regulator with its target promoter region. catBC pro- moter activation by CatR. Matthew R. Parsek, Rick w. Ye, Pattle Pun, and A. M. Chakrabarty

,1361 A hammerhead ribozyme inhibits the proliferation of an RNA coliphage SP in Escherichia coli. Yoshio Inoku- chi, Noriko Yuyama, Akikazu Hirashima, Satoshi Nishikawa, Jun Ohkawa, and Kazunari Taira

1417 Alteration of lipid profiles in plasma of transgenic mice expressing human lipoprotein lipase. Ming-Sun Liu, Frank R. Jirik, Renee C. LeBoeuf, Howard Henderson, Law- rence W. Castelluni, Aldons J . Luis , Yuunhng Ma, Zan J . Forsythe, Hanfang Zhang, Elizabeth Kirk, John D. BrunzeU, and Michael R. Hayden

1425 S p l is a critical factor for the monocytic specific expression of human CD14. Dong-Er Zhqng, Christopher J. Hetherington, Shencao Tan, Suzan E. Dzzennis, Davld A. Gonzalez, Hui-Min Chen, and Daniel G. Tenen

11514 Analysis of androgen receptor-DNA interactions with receptor proteins produced in insect cells. Pekka J . Kal- lw, Jorma J . Paluimo, Merja Mehto, and OUi A. Janne

11530 Human cathepsin 5: chromosomal localization, gene structure, and tissue distribution. Gw-Ping Shi, Andrew

Lemere, John S. Munger, and Harold A. Chapman C. Webb, Karen E. Foster, Joan H. M. Knoll, Cynthia A.

11542

11563

11656

11663

11672

11674

*lo983

010987

11054

11073

11098

11102

11 108

11178

11186

11196

11233

374

400

Incorporation of adenovirus into a ligand-based DNA carrier system results in retention of original receptor specificity and enhances targeted gene expression. George Y. Wu, Peili Zhan, Lillian L. Sze, Arielle R. Rosenberg, and Catherine H. Wu DNA distortion and nucleation of local DNA unwinding within sigma-54 (P) holoenzyme closed promoter com- plexes. Lorna Morris, Wendy Cannon, Felix Claverie-Martin, Sara Austin, and Martin Buck

Negative modulator of the ,rat DZ dopamine receptor gene. Takashi Minowa, Mart T. Minowa, and M. Mural Mouradian

Characterization of the genomic structure, chromo- somal location, promoter, and developmental expres- sion of the u-globin transcription factor CP2. Steven L. Swendeman, Charles Spielholz, Nancy A. Jenkins, Debra J . Gilbert, Neal G. Copeland, and Michael Sheffery

Molecular characterization and expression of a gene encoding a Staphylococcus aureus collagen adhesin.

son, Bengt Gus , Lech M. Switalski, Kristina Wiberg, Martin Vol. 267 (1992) 4766-4772. Joseph M. Patti, Hans Jons-

Lindberg, and Magnus HMk

Thyroid hormone receptor modulates the expression of the rabbit cardiac sarco(endo)plasmic reticulum Ca'+- ATPase gene. Vol. 269 (1994) 1460-1467. Angel Zar- ain-Herzberg, John Marques, Drew Sukouich, and Muthu Periasamy

PROTEIN CHEMISTRY AND STRUCTURE The substituted benzimidazolone NS004 is an opener of the cystic fibrosis chloride channel. Valentin K. Grib-

Nicholas A. MeanweU, and Michel Lazdunski koff, Guy Champigny, Pascal Barbry, Steven I. Dworetzky,

Chemical characterization of Aj3 17-42 peptide, a com- ponent of diffuse amyloid deposita of Alzheimer disease. Eric Gowing, Alex E. Roher, Amina S. Woods, Robert J . Cotter, Michael Chaney, Sheila P. Little, and Melvyn J . BaU

Mutagenic studies of the interaction between the aspar- tate receptor and methyltransferase from Escherichia coli. Michael J . Shupiro and Daniel E. Koshland, Jr.

The complete primary structure for a novel laminin chain, the laminin B l k chain. Donald R. Gerecke, D. Wove Wagman, Marie-France Champliaud, and Robert E. Burgeson

Isolation and sequencing of insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Si- mon). Gary B. Quistad and Wayne S. Skinner

Probing the retinol-binding site of bovine @-lactoglob- ulin. Yunje Cho, Carl A. Butt, and Lindsay Sawyer

Mutational analysis of the metal sites in an LIM do-

Louis, Jay I. Olsen, Darrell R. Davis, Dennis R. Winge, and main. James W. Michelsen, Andrew K. Sewell, Heather A.

Mary C. Beckerle

Functional expression and site-directed mutagenesis of a synthetic gene for u-bungarotoxin. Julie A. Rosenthal, Sigmund H. Hsu, Dinesh Schneider, Lisa N. Gentile, Norma J. Messier, Charles A. Vaslet, and Edward Hawrot

A peptide encoding the c-Jun 6 domain inhibits the activity of a c-Jun amino-terminal protein kinase. Vic- tor Adler, Tino Unlap, and Andrew s. Kraft

Identical mutations at corresponding positions in two

Joakim Bjorkman, R. Alan Binnie, L. Brent Cole, Huide Zhang, homologous proteins with nonidentical effects. A.

Mark A. Hermodson, and Sherry L. Mowbray

Site of fasciculin interaction with acetylcholinesterase. Zoran Radii, Rosarw Duran, Daniel C. VeUom, Ying Li, Carlos Cervenunsky, and Palmer Taylor

Characterization of a stable form of human meizo- thrombin derived from recombinant prothrombin (R156A. R271A, and R284A). Hekne C. F. C&, WiUem K. Stevens. Laszlo Baizar. David K. Banfield. Michael E. Nesheim, and Ross f 2. MacGibivray

,~ I

A partial structural repeat forms the heterodimer self-

A. Weed, Bernard G. Forget, and Jon S. Morrow association site of all 6-spectrins. Scott P. Kennedy, Scott

Indicates a Communication

xi

11601 A role for the C2 domain of factor VIII in binding to von Willebrand factor. Evgueni L. Saenko, Midori S h i m K. J. Rajalokshmi, and Dorothea Scandella

11606 Conformation of Ca2+-ATPase in two crystal forms.

y1ene)triphmphate). 'and chromium(III)-ATP on crys- Effects of Cap+, thapsigargin, adenosine S'-@,r-meth-

tallization. David L. Stokes and Jean-Jacques hap&

11614 Self-assembly of collagen I from a proband homozygous for a mutation that substituted eerine for glycine at position 661 in the a2(I) chain. Possible relationship between the effects of mutations on critical concentra- tion and the severity of the phenotype. Anne M. Romanic, Loretta D. Spotila, E&roAdachi, Jurgen Engel, Yoshw Hojirna, and Darwin J . Proekop

11673 Long range propagation of conformational changes in integrin u d 8 . Vol. 268 (1993) 23087-23092. Xiooping Du, Minyi Gu, John W. Weisel, Chandrasekuran Nagaswami, Joel S. Bennett, Ron Bowditch, and Mark H. Ginsberg

11456 Disullide mutants of the binding domain of the rat low affinit nervefowth factor receptor (p7SN(IPR). Anne N. Badrwin and m M Shooter

11462 Stimulation of calcification of growth plate cartilage matrix vesicles by bindin to type I1 and X collagens. Thorsten Kirsch and Roy E. $uthier

11484 Yolk rotein factor 1 is a Droeo hila homolog of Ku, the DKA-binding subunit of a DgA-de ndent protein kin- from humans. Dough B. J a c o r and Pwter C. Wensrnk

11584 Self-assembly into fibrile of collagen I1 by enzymic cleav e of recombmant procoll en II. Lapi pernod, critic3concentration aqd mo hzo of fibrils differ from collagen 1. Andrzel F e r t z Ak%ander L. Sieron, Yoshw Hojima, A r q Ganguly, and barwin J. Prockop

11590 Zinc finger domains and phorbol ester harmaco hore. Analysis of binding to mutated form of protein gin- Marcelo G. Kazanietz, Xose R. Bustelo, Marion0 Barbae@, C I and the vav and e-rat proto-oncogene products. Walter Kolch, H a d M i s c h Garry Wong, George R. Petttt, Jay D. Bruns, and Peter M. Bkumberg

Abraham, Edward H., 11224 Adachi, Eijiro, 11614 Adler, Victor, 11186 Aebersold, Ruedi, 10975 Agarwal, Kamlesh C., 11025 Aguan, Kripamoy, 11442 Amara, Jane F., 11224 Ananth, Sandya, 11503,

Anderson, Richard A., 11547 Anderson, Vernon E., 11025

Apicella, Michael A., 11162 Anger, Marielle, 11147

Ausiello, Dennis A., 11224 Austin, Sara, 11563 Baba, Shigeaki, 11498 Bajzar, Laszlo, 11374 Baldwin, Anne N., 11456 Ball, Melvyn J., 10987 Ballotti, Robert, 11449 Banfield, David K., 11374 Barbacid, Mariano, 11590 Barharas, Ronald, 11572 Barbry, Pascal, 10983 Barrans, Alain, 11572 Batt, Carl A,, 11102 Barter, Robert C., 11470

Beckerle, Mary C., 11108 Becker, Thomas C., 11523

Beddow, Adrienne L., 11285 BeltrandelRio, Hector, 11523

Ben, Raj K., 11442 Bennett, Joel S., 11673

Beylot, Michel, 11025 Bhatnagar, Rajiv S., 11045 Binnie, R. Alan, 11196 Bizub-Bender, Diane, 11170 Bjorkman, A. Joakim, 11196 Blumberg, Peter M., 11590 Bliiml, Klaus, 11537 Bolen, Joseph B., 11409 Bonner, Tom I., 11065 Boulton, Ten G., 11648 Boutillon. Marmerite-Marie.

11509

. - 11381

Boyd, Beth, 11138 Bowditch, Ron, 11673

Breittmayer, Jean-Philippe, 11449

Brennan, Caroline H., 11442 Bmnengraber, Henri, 11025 Bruns, Jay D., 11590 Brunzell, John D., 11417 Buck, Martin, 11563

Burgeson, Robert E., 11073 Burstein, Haim, 11170 Bustelo, X086 R., 11590 Bureda, Rosa J., 11018 Cai, Ji-Qun, 11640 Cameron, Craig E., 11 170 Campbell, Patrick, 11503,

Cannon, Wendy, 11563 Cantiello, Horacio F., 11224 Capps, G. George, 11634 Carling, David, 11442 Carman, George M., 11018 Cassidy, Laura E., 11523 Castellani, Lawrence W.,

Cervenansky, Carlos, 11233 Chakrabarty, A. M., 11279 Champigny, Guy, 1098.3 Champliaud, Marie-France,

Chaney, Michael, 10987 Chap, Hugues, 11572 Chapman, Harold A,, 11530 Chellaiah, Arasu T., 11620 Chen, Gi-Chung, 11381 Chen, Hui-Mia, 11425

Christiansen, Kent, 11367 Cho, Yunje, 11102

Ciliberto, Gennaro, 10991 Clark, Avril E., 11578 Clark, Samuel, 11523 Clavene-Martin, Felix, 11563 Cole, L. Brent, 11196 Collet, Xavier, 11572

Copeland, Terry, 11170 Copeland, Neal G., 11663

C&, HBlbne C. F., 11374 Cotter, Robert J., 10987 Coverley, Jennifer A., 11470 Cuillel, Martine, 11147 Czar, Michael J., 11155 Daiho, Takashi, 11060

David, France, 11025 Danaher, Maria, 11011

Davis, Darrell R., 11108 Davison, Matthew D., 11442 Deakin, Jon A,, 11208, 11216 Deibel, Martin R., Jr., 11155 Delmastro, Paolo, 10991 Denninghoff, Karl, 11133

Dittmar, Kurt D., 11155 Diegel, Michael L., 11409

Du. Xiaoping, 11673

11509

11417

11073

AUTHOR INDEX

Dubois, Patrice M., 11409 Dukes, Iain D., 10979 Dupont, Yves, 11147

Dworetzky, Steven I., 10983 Duran, Rosario, 11233

Dziennis, Suzan E., 11425 Egerton, Mark, 11435

Engstrom, Jeffrey J., 11162 Engel, Jurgen, 11381, 11614

Ercole, Liliana B., 11224 Fallon, Robert J., 11011 Feizi, Ten, 11555 Ferber, Sarah, 11523

Fetzner, Susanne, 11254 Fertala, Andrzej, 11584

Fischer von Mollard, Fini, M. Elizabeth, 11291

Gabriele, 10971 Fisette, Philip L., 11547 Fitzpatrick, F. A., 11269 Forget, Bernard G., 11400 Forsythe, Ian J., 11417 Foster, Karen E., 11530 Fricker, Lloyd D., 11192 Frimpong, Kenneth, 11478 Gallagher, John T., 11208,

Ganguly, Arupa, 11584 Geiger, Otto, 11090 Gellert, Martin, 11081 Gentile, Lisa N., 11178 Gerecke, Donald R., 11073 Gibson, Bradford W., 11162 Gierse, J. K., 11269 Gilbert, Debra J., 11663 Ginsberg, Mark H., 11673 Girard, Marie T., 11291 Glushka, John, 11090 Gonzalez, David A., 11425

Goto, Sachiyo, 11274 Gordon, Jeffrey I., 11045

Gotoda, Takanari, 11673 Gowing, Eric, 10987 Grabowski, Gregory A,, 10975 Grace, Marie E., 10975 Graziani, Rita, 10991 Gregory, Richard J., 11224 Grell, Ernst, 11261 Gribkoff, Valentin K., 10983 Gu, Minyi, 11673 Guss, Bengt, 11672 Hagting, Anja, 11391

11216

Hammarback, James A,,

Harpel, Mark R., 11114

Hartman, Fred C., 11114 Harrison, Richard A., 11555

Hasegawa, Kaori, 11274 Hashiramoto, Mitsuru,

Hawrot, Edward, 11178 Hayden, Michael R., 11417 Henderson, Howard, 11417 Henricksen, Leigh A,, 11121 Hermohn, Mark A,, 11196 Hetherington, Christopher J.,

Hirashima, Akikazu, 11361 Hojima, Yoshio, 11381,

Holmes, Eric H., 11327 Holman, Geoffrey D., 11578

Homcy, Charles J., 11672

Hosomi, Yoichi, 11498 Hwk, Magnus, 11672

Hsu, Sigmund H., 11178 Hughes, Steven D., 11523 Hutchison, Kevin A., 11155

Igarashi, Kazuei, 11037 Imai, Shoichi, 11640 Inaba, Toshimori, 11673

Inokuchi, Yoshio, 11361 Inaguma, Yutaka, 11274

Islam, Md Omedul, 11640

Jackson-Machelski, Emily,

Jacoby, Douglas B., 11484 Jahn, Reinhard, 10971 Jiinne, Olli A,, 11514

Jenkins, Glenn H., 11547 Jaspard, Beatrice, 11572

Jenkins, Nancy A., 11663 Jirik, Frank R., 11417 Johnson, John H., 11523 Jonsson, Hans, 11672

Kakinuma, Shizuko, 11037 Kakinuma, Yoshimi, 11037 Kalinowski, Stephen S.,

Kallio, Pekka J., 11514 Kanazawa, Tohru, 11060 Kasuga, Masato, 11349,

Kato, Kanefusa, 11274 Kawahara, Yasuhiro, 11628

11492

11349,11578

11425

11584,11614

11045

11201

11498,11578

Kawamura, Masako, 11673 Kazanietz, Marcelo G., 11590 Kazumi, Tsutomu, 11349

Kennedy, Scott P., 11400 Kelvin, David J., 11065

Kiarash, Arash, 11138 Khokhlatchev, Andrei, 10971

Kiener, Peter A., 11409 Kirk, Elizabeth, 11417 Kirsch, Thorsten, 11462 Kbhimoto, Miyako, 11349

Knudsen, Birgitta R., 11367 Knoll, Joan H. M., 11530

Koga, Takaki, 11640 Kolch, Walter, 11590 Konings, Wil N., 11391 Konishi, Kiyoshi, 11037 Kornberg, Arthur, 10996 Koshland, Daniel E., Jr.,

Kowalski-Chauvel, Aline,

Kozyavkin, Sergei A., 11081 Kraft, Andrew S., 11186 Krah, Regis, 11081 Kriimer, Reinhard, 11240 Kreil, Giinther, 10967

Kunji, Edmund R. S., 11391 Kumar, Sharad, 11318

Lacapire, Jean-Jacques,

Larimer, Frank W., 11114 Lake, James A., 11081

Lautens, Laura L., 11065 Lawrence, John C., Jr., 11595 Lawson, Alexander M., 11555 Lazdunski, Michel, 10983

LeBoeuf, Renee C., 11417 Leach, Karen L., 11155

Lee, Frank K. N., 11162 Lee, Yong-Hwan, 11002 Leenhouts, Kees J., 11391 Lehmann, Martin, 11254

Lemere, Cynthia A,, 11530 Lei, Jonathan, 11170

Liao, James K., 11672 Li, Ying, 11233

Lin, Feng, 11312 Lin, Kai, 11002 Lindberg, Martin, 11672 Lingens, Franz, 11254

Little, Sheila P., 10987 Lingwood, Clifford A., 11138

Liu, Ming-Sun, 11417

11054

11449

11606