IN VITRO ASSAY : DIRECT VISUALIZATION

OF CITREX® MOLECULE ACTION ON MICROORGANISMS

HAROLD EDUARDO DURANGO G.HAROLD EDUARDO DURANGO G.Bacteriologist, Medical Mycology SpecialistProfessor of Microbiology and Parasitology.Medicine Faculty, U. of A.

JOSE IGNACIO BARGUIL P.JOSE IGNACIO BARGUIL P.Bacteriologist, Food Microbiology Specialist.Food Protection Technical Consultant.Consultant for Banco Interamericano de Desarrollo, BID.Technical Director, Continua Consultores.

Pediatrics Infectology Research Laboratory. H.U.S.V.PPediatrics Infectology Research Laboratory. H.U.S.V.P

METHODOLOGY

• Bacterial suspensions containing 106 CFU/ml in a 1,000 ppm CITREX® solution are prepared. Allow to work for 15 and 30 minutes. Both direct and Gram stain smears are prepared. Observations are filmed.

Staphylococcus aureusbefore and after CITREX ® exposure.

before 30 minutes

Salmonella typhimurium

prior to CITREX ® exposure.

Salmonella typhimurium

after a 15-minute CITREX® exposure

Gram negative rod destruction is remarkable, together with increased amorphous protein material resulting from bacterial destruction.

Gram negative rod destruction is remarkable, together with increased amorphous protein material resulting from bacterial destruction.

Salmonella typhimurium

after a 30-minute CITREX® exposure

Blastoconidia from a Candida albicans pure culture, prior to Citrex® exposure.

Yeast structural deterioration after a 15-minute Citrex® exposure.

Yeast structural deterioration after a 30-minute Citrex® exposure.

Aspergillus flavus heads and conidia from a pure culture, prior to

Citrex® exposure.

Aspergillus flavus head structural deterioration after a 15-minute Citrex®

exposure.

Aspergillus flavus head structural deterioration after a 30-minute Citrex® exposure.

DIRECT TECHNIQUE

• Direct tests are performed over a pure organism culture smear (Salmonella typhimurium, Staphylococcus aureus, and Candida albicans). 400 ppm Citrex® solution drops are added. Reading is practiced after 15 and 30 minutes.

Salmonella typhimurium,Salmonella typhimurium, Staphylococcus aureus, Staphylococcus aureus, and Candida albicansand Candida albicans,

prior to Citrex® exposure.

Salmonella typhimurium,Salmonella typhimurium, Staphylococcus aureus, Staphylococcus aureus, and Candida albicansand Candida albicans

after a 15-minute Citrex® exposure.

Salmonella typhimurium,Salmonella typhimurium, Staphylococcus aureus, Staphylococcus aureus, and Candida albicansand Candida albicans

after a 30-minute Citrex® exposure.