Vivid™ Plasma Separation membrane

P R O T E C T W H A T M A T T E R S - E V E R Y D A Y

Pall’s Vivid Plasma Separation membrane

is produced using a patented process

resulting in a highly asymmetric

membrane. Its asymmetry enables the

capture of whole cells for the generation

of plasma, negating the need for cell

removal by centrifugation.

One Step Plasma Separation From Whole Blood

Vivid™ Plasma Separation membrane

• High plasma yield

Achieve high quality plasma in less than two minutes with ≥ 80% plasma yield.

• Low analyte binding

Low, non-specific binding of common diagnostic biomarkers and target analytes.

• Dependable performance

Efficient removal of whole blood cellular components, including red cells, white cells, and platelets.

• Low hemolysis

Hemolysis levels significantly lower than glass fiber media generated plasma.

• Device integration

Compatible with point of care (POC) and point of use (POU) diagnostic platforms such as lateral flow test strips and microfluidics.

Optimized, highly efficient membrane for one-step plasma separation from whole blood without the need for centrifugation

2 3

The highly asymmetric nature of our Vivid Plasma Separation (PS) membrane allows the cellular components of blood (red cells, white cells, and platelets) to be captured within the larger pores on the upstream side of the membrane. Cells do not lyse. Enabling the plasma to flow through the smaller pores on the downstream side of the membrane. This rapid separation process yields plasma similar in HPLC and SDS-PAGE profiles to traditional centrifuged plasma in less than two minutes.

Non-specific binding of clinically relevant biomarkers is a concern when working with porous materials in diagnostic applications. Whole blood processed through the Vivid PS membrane has shown equivalent 2D SDS-PAGE protein profiles for the cardiac biomarker Troponin I as compared to centrifuged plasma. This data indicates that the protein concentration of clinical biomarkers is not reduced when processed through the membrane,

thus making it an ideal material for diagnostic applications.

The Vivid PS membrane provides high plasma yields, lowering the amount of starting whole blood needed. For POC and POU diagnostic applications, this is advantageous as whole blood volumes can be minimized resulting in smaller amounts of blood needed from patients or animals. The Vivid PS membrane can yield over 80% of the theoretical plasma available, while comparable glass fiber yields are in the region of 30-50%.

Captured Red Cells

Separated Cell-Free Plasma

Standard Deviation

Coefficient of Variation

Performance

Vivid™ Plasma Separation membrane

5

Specifications

Typical Membrane Characteristics

Base Material

GFAysmmetric Polysulfone

Grade Thickness

mils μm

12.99 +/- 0.79 330 +/- 20

Membrane Void Volume

1X

Plasma Separation Time (sec)

≤ 2 mins

Plasma Recovery (%)

≥ 60 %

100

80

60

40

20

01.0 1.5 2.0 2.5 3.0

Low HTC

1.0 1.5 2.0 2.5

Average HTC

1.0 1.5 2.0 2.5

High HTC

Blood Volume Applied (in terms of Void Volumes)

Pla

sma

Rec

ove

ry(%

of

Ava

ilab

le)

20

15

10

5

0

Sep

arat

ion

Tim

e (M

in)

Recovered Plasma Separation Time

Achieve high plasma yields with Vivid PS membrane.

The percent of plasma recovered from different volumes of blood does not depend on the blood volume applied to the media. Graph 1 shows that the separation time increases dramatically with increasing blood volume applied to the media.

Graph 2 shows how Vivid PS membrane does not bind to clinically relevant protein biomarkers from plasma samples. All plasma samples were generated from the same sample of fresh EDTA blood spiked with Troponin I at 1 ng/mL. Protein concentration in each sample was measured in triplicate.

Note: The sample blood volume capacity of Vivid PS membrane is defined as the amount of whole blood per cm2 of medium that is separated in less than 2 minutes by the medium without hemolysis. The blood volume capacity of the medium is directly related to its void volume. The calculated void volume of Vivid PS membrane is ~20 ± 1μL/cm2. With knowledge of the void volume within the Vivid PS membrane, and the

1.6

1.4

1.2

1.0

0.8

0.6

0.4

0.2

0Centr. Control GR (1) GR (2) GF

Tro

po

nin

I Co

ncen

trat

ion,

ng/m

L M

easu

red

by

ELI

SA

Plasma Samples of 1 ng/mL Spiked Blood

specific membrane post-treatments utilized, we express recommended blood volume capacities that generate consistent plasma separation without hemolysis. These blood sample volume recommendations must be followed in order to achieve optimal plasma quality.

Blood Volume (μL / cm2)

20

Typical Performance Characteristics

GXAysmmetric Polysulfone

12.99 +/- 0.79 330 +/- 20 1.5X ≤ 2 mins ≥ 60 %20 - 30

GRAysmmetric Polysulfone

12.99 +/- 0.79 330 +/- 20 2X ≤ 2 mins ≥ 80 %40 - 50

Note: The product performance characteristics outlined above are determined using EDTA collected whole blood with typical hematocrit content of 45.6 %. For effective sample preparation it is important to ensure that the membrane lies flat, that no end contact occurs, and the edges are sealed by compression. In addition, the underlying material must have enough capillary force to wick the plasma from the Vivid PS membrane.

GF

GX

GR

Grade Dimensions

Small blood volume applications, such as finger sticks in microfluidic and lateral flow format POC devices. This material is untreated and may exhibit higher hemolysis levels than other grades.

Small blood volume applications, such as finger sticks in microfluidic and lateral flow format POC devices. Also compatible with electrochemical analyte detection. Post-treatment helps to minimize hemolysis.

Larger blood volume applications, such as lateral flow immunochromatographic devices. Post-treatment facilitates larger blood volumes with reduced hemolysis.

Graph 1: To show plasma recovery (blue columns) and separation time (blue rhombus) as a function of blood volume applied.

Graph 2: Troponin I concentration after filtration through Vivid PS membrane.

Vivid™ Plasma Separation membrane

6 7

Why do you have three grades of Vivid PS membrane? What is the difference between them?

The three grades are designed specifically for application use:

• GF is not post treated and is designed for small volumes of blood.

• GX grade is designed for small blood sample volumes (eg. fingerstick), compatibility with microfluidic devices and high sensitivity detection regimes (eg. electrochemical).

• GR material is designed for larger volumes of blood typically found in lateral flow applications.

What is the orientation of Vivid PS membrane?

• The asymmetric structure of Vivid is apparent by visual inspection. There is a shiny side and a dull side. To achieve plasma separation, blood must be applied to the dull side.

?Frequently asked questions

• A major problem when using glass is the hemolysis of samples. Vivid PS membrane does not exhibit hemolysis of the blood sample.

• Target analytes can bind non specifically to glass microfibers thereby potentially reducing assay sensitivity. Vivid PS membrane does not bind target analytes such as proteins.

• Vivid PS membrane has a higher plasma product efficiency than glass. – ie. for the same amount of blood the yield of plasma is greater when compared to glass microfiber.

• Glass is difficult to fabricate into devices. Over-compression causes glass fiber cracking which increases the chance of sample hemolysis.

What are the advantages of Vivid PS membrane over glass microfiber?

Why would I use Vivid PS membrane, rather than a centrifuge, for blood separation?

• Centrifugal blood separation is equipment dependent and therefore cannot be utilized in a POC situation. Blood separation using Vivid PS membrane has no equipment dependency and produces plasma of equivalent quality.

?

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International Offices

Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria, Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico, the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden, Switzerland, Taiwan, Thailand, the United Kingdom, the United States and Venezuela. Distributors in all major industrial areas of the world.

The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.

© 2019, Pall Europe. Pall, , Vivid, and Leukosorb are trademarks of Pall Corporation. ® indicates a registered trademark in the USA. Protect What Matters - Every Day is a service mark of Pall Corporation.

Pall Corporate Headquarters 25 Harbor Park Drive Port Washington, NY 11050 USA

(877) 367-7255 phone

Pall European Headquarters Pall International Sàrl Avenue de Tivoli 3 1700 Fribourg Switzerland

+41 (0) 26 350 53 00 phone

Pall Asia-Pacific Headquarters 1 Science Park Road, #05-09/15East Wing, The CapricornSingapore Science Park II Singapore 117528

+65 6389 6500 phone

Ordering Information

Part Number

T9EXPPA0200S00A

T9EXPPA0200S00X

T9EXPPA0200S00R

Vivid GF Plasma Separation membrane

Vivid GX Plasma Separation membrane

Vivid GR Plasma Separation membrane

Description

GF

GX

GR

Grade Dimensions

8” x 11”

8” x 11”

8” x 11”

Pkg

1 Sheet

1 Sheet

1 Sheet

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Custom roll, sheet, and disc sizes available upon request.