Vitamin D and Cancer

Dorothy Teegarden, Ph.D.Purdue University

Professor and Associate Head for Research, Department of Nutrition Science

Lead, Cancer Prevention and Control Program, Oncological Sciences Center

Director, Interdisciplinary Cancer Prevention Internship Program

Vitamin D is Metabolized and Acts Like a Hormone

Blood

25 hydroxyvitamin DStatus

Indicator

1, 25 hydroxyvitamin DActive

Hormone

Sunlight

Visit 1 25OHD levelsN = 206

Raw Data SheetsAssay Method: LC/MS-MRMAssay Date: 10/11 - 10/13/10Sample volume: 250 uL

Limitof

Results Key: Quantification

25-(OH) VD310pg/mL (0.025nM)

25-(OH) VD210pg/mL (0.024nM)

Assay Results:

Client Concentration [nM]Sample # 25-(OH) VD3 25-(OH) VD2 25-(OH) VD [Total]0591SE04 151.5 1.8 153.40261SE04 80.8 1.3 82.10391SE04 118.1 35.8 153.8

Assay Notes:

1Total Samples Assayed: 74

2Validation of ResultsQuality Control (QC) samplesNIST Standard Reference Material 972

Name 25-(OH) VD3 25-(OH) VD2  nM nM

SRM 972 Level 3 47.5 63.8SRM 972 Level 3 51.2 66.0SRM 972 Level 3 51.4 66.8SRM 972 Level 3 52.9 65.8SRM 972 Level 3 47.5 62.4

average 50.1 65.0Standard derivation 2.47 1.81

NIST Reference Values; Standard Reference Material 972 Level 3NIST Level 3 25-(OH) VD3 25-(OH) VD2

  nM nMAverage assay

result 50.1 65.0Reference value 46.2±2.8 64.1±4.8

% Difference 8.4% 1.3%

Multiple SheetsDoes not distinguish between first and second visitsExcess information on sheet not needed for analysisSelect data, down load, merge for each analysis

Results in Format for AnalysisPatient Barcode

BD1: FileNo

BD1: AliquotID

BD1: 25-(OH) VD3 (nM)

BD1: 25-(OH) VD2 (nM)

BD1: 25-(OH) VD (nM)

BD2: FileNo

BD2: AliquotID

BD2: 25-(OH) VD3 (nM)

BD2: 25-(OH) VD2 (nM)

BD2: 25-(OH) VD (nM)

1 10011SE04 108.2 4.9 113.2- - - - -

2 10021SE04 69.1 1.7 70.8- - - - -

3 10031SE04 91.1 1.4 92.5- - - - -

4 10041SE04 78 3.7 81.7- - - - -

5 10051SE04 60.3 1.5 61.8- - - - -

6 10061SE04 171.2 1.2 172.4- - - - -

7 10071SE04 134.8 4.5 139.3- - - - -

9 10091SE04 67.7 1.5 69.2 10092SE04 92.9 1.7 94.6

-One excel sheet-Linked to clinical data-Formatted for easy analysis

ResultsData Summary of

the First Visit

Diagnosis25-(OH) VD3

(nM)25-(OH)

VD2 (nM)25-(OH) VD Total (nM)

Sample size

Colorectal Healthy Polyps

87.983.281.4

48.66.7

91.991.888.1

318887

Leveraging CCE HUB and Available Results

• Vitamin D and metabolomics

• Breast CCE

• International Breast Cancer and Nutrition

–Global