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clxxviii

VIII. ABSTRACT

The efficacy of recombinant DNA, live recombinant BCG and BCG

vaccine was tested in a group of calves selected from herds with known

negative tuberculosis status. To assist in the evaluation, five groups of 8

calves each, aged approximately 3-6 months were injected

subcutaneously with either 1x106 CFU of recombinant BCG,

Conventional BCG vaccine and intramuscularly with 1000µg of

recombinant DNA expressing Rv3881c protein. A control group received

PBS via the same route. The immune status was monitored using the

Interferon-γ assay, Flowcytometry study of CD4 and CD8 cells and

Cytokine IL-4 capture ELISA.

The immune status scores with respect to IFN-γ, IL-4 and T cell

subtypes of individual animals were generally much higher in the calves

injected with recombinant BCG vaccine than what was experienced with

the Conventional BCG, recombinant DNA vaccine and control model and

which were statistically significant. It can be concluded that under the

prevailing conditions the r-BCG vaccines expressing Mycobacterial

protein designated as Rv3881c was able to show higher immune status

in calves than Conventional BCG and rDNA vaccine.

iii